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In vitro culture studies on Stevia rebaudiana


Shoot apex, nodal, and leaf explants of Stevia rebaudiana Bertoni can regenerate shoots when cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA; 8.87 μM) and indole-3-acetic acid (5.71 μM). Rooting of the in vitro-derived shoots could be achieved following subculture onto auxin-containing medium. A survival rate of 70% was recorded at the hardening phase on the substrate cocopeat. The presence of the sweet diterpene glycosides, viz. stevioside and rebaudioside, was confirmed in the in vitro-derived tissues of Stevia using HPTLC techniques. Callus cultured on agar-solidified MS medium supplemented with BA (8.87 μM) and indole-3-butyric acid (9.80 μM) showed the highest sweetener content.

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Correspondence to Usha Mukundan.

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Sivaram, L., Mukundan, U. In vitro culture studies on Stevia rebaudiana . In Vitro Cell.Dev.Biol.-Plant 39, 520–523 (2003).

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Key words

  • Stevia rebaudiana
  • stevioside
  • micropropagation
  • tissue culture