Abstract
Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained growth of callus tissue. Cultures produced anticancer lignans podophyllotoxin, 4′-demethylpodophyllotoxin and podophyllotoxin 4-O-glucoside at levels similar to those found in the expiant material as assayed by high performance liquid chromatography. The relative proportions of podophyllotoxin and 4′-demethyl-podophyllotoxin were markedly influenced by the presence of plant growth regulators. Particularly high levels of podophyllotoxin were associated with growth regulator induced tissue differentiation.
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Communicated by W. Barz
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Heyenga, A.G., Lucas, J.A. & Dewick, P.M. Production of tumour-inhibitory lignans in callus cultures of Podophyllum hexandrum . Plant Cell Reports 9, 382–385 (1990). https://doi.org/10.1007/BF00232404
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DOI: https://doi.org/10.1007/BF00232404