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Probing the adverse temperature dependence in the static fluorescence quenching of BSA induced by a novel anticancer hydrazone

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Abstract

A novel hydrazone, 2-hydroxy-N′-(3-hydroxybenzylidene) benzohydrazide (HHB), has been designed, synthesized and characterized. HHB was designed to be an analogue of 311 and PIH with potential anticancer activity, and the IC50 towards HeLa cell was about 3.46 × 10−5 mol−1 L. The interactions of HHB with bovine serum albumin (BSA) had been investigated systematically by spectroscopy, electrochemistry, and molecular modeling under simulative physiological conditions. HHB bound BSA in the sub-domains IIA to form a ground-state complex, inducing the quenching of the intrinsic fluorescence emission, the change of absorption spectrum and the increase of electrical resistance of BSA. An adverse temperature dependence in the fluorescence quenching was detected and discussed to be a reasonable consequence of the big Ea requirement to overcome the obstructive amino acid residues in the entrance to the binding site, which were closely related to the natural structure of BSA and the molecular shape of HHB. The impact of metal ions, including Fe2+, Fe3+, Cu2+, Mg2+, Zn2+, Ca2+ and Al3+, towards the interactions of HHB and BSA has been investigated and they were found to affect the HHB-BSA interactions in a mild way.

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Authors and Affiliations

  1. College of Chemical and Environmental Engineering, Yangtze University, Jingzhou, 434023, P. R. China

    Jin-Qiang Tong, Qing Li, Yi Liu & Jie Dai

  2. State key laboratory of Virology and Key laboratory of Analytical Chemistry for Biology and Medicine (MOE), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, P. R. China

    Fang-Fang Tian, Li-Li Li, Chen Xiang, Yi Liu & Feng-Lei Jiang

Authors
  1. Jin-Qiang Tong
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  2. Fang-Fang Tian
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  3. Qing Li
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  4. Li-Li Li
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  5. Chen Xiang
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  6. Yi Liu
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  7. Jie Dai
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  8. Feng-Lei Jiang
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Corresponding authors

Correspondence to Yi Liu or Feng-Lei Jiang.

Additional information

Electronic supplementary information (ESI) available: The characterization data of HHB, containing the IR spectrum, 1H NMR and the MS of HHB. See DOI: 10.1039/c2pp25162k

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Tong, JQ., Tian, FF., Li, Q. et al. Probing the adverse temperature dependence in the static fluorescence quenching of BSA induced by a novel anticancer hydrazone. Photochem Photobiol Sci 11, 1868–1879 (2012). https://doi.org/10.1039/c2pp25162k

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  • DOI: https://doi.org/10.1039/c2pp25162k

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