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Spectroscopic properties and bacteriochlorophyll c isomer composition of extramembranous light-harvesting complexes in the green sulfur photosynthetic bacterium Chlorobium tepidum and its CT0388-deleted mutant under vitamin B12-limited conditions

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Abstract

The effects of exogenous vitamin B12 on the green sulfur photosynthetic bacterium Chlorobium (Chl.) tepidum were examined. Wild-type cells and mutant cells lacking a gene CT0388 (denoted as VB0388) of Chl.tepidum were grown in liquid cultures containing different concentrations of vitamin B12. The VB0388 cells hardly grew in vitamin B12-limited media, indicating that the product of CT0388 actually played an important role in vitamin B12 biosynthesis in Chl. tepidum. Both wild-type and VB0388 cells in vitamin B12-limited media exhibited absorption bands and CD signals at the Qy region that were shifted to a shorter wavelength than those of cells grown in normal media. BChl c isomers that had S-stereochemistry at the 31-position tended to increase in Chl. tepidum grown in vitamin B12-limited media.

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References

  1. R. E. Blankenship, J. M. Olson and M. Miller, Antenna complexes from green photosynthetic bacteria, in Anoxygenic Photosynthetic Bacteria, ed. R. E. Blankenship, M. T. Madigan and C. E. Bauer, Kluwer Academic Publishing, Dordrecht, 1995, p. 399

    Chapter  Google Scholar 

  2. J. M., Olson, Chlorophyll organization and function in green photosynthetic bacteria Photochem. Photobiol. 1998 67 61–75

    Article  CAS  Google Scholar 

  3. T. Miyatake and H., Tamiaki, Self-aggregates of bacteriochlorophylls-c, d and e in a light-harvesting antenna system of green photosynthetic bacteria: effect of stereochemistry at the chiral 3-(1-hydroxyethyl) group on the supramolecular arrangement of chlorophyllous pigments J. Photochem. Photobiol., C 2005 6 89–107

    Article  CAS  Google Scholar 

  4. T. S. Balaban, H. Tamiaki and A. R., Holzwarth, Chlorins programmed for self-assembly Top. Curr. Chem. 2005 258 1–38

    Article  CAS  Google Scholar 

  5. H., Tamiaki, Self-aggregates of natural and modified chlorophylls as photosynthetic light-harvesting antenna systems: substituent effect on the B-ring Photochem. Photobiol. Sci. 2005 4 675–680

    Article  CAS  Google Scholar 

  6. H. Tamiaki, R. Shibata and T., Mizoguchi, The 17-propionate function of (bacterio)chlorophylls: biological implication of their long esterifying chains in photosynthetic systems Photochem. Photobiol. 2007 83 152–162

    CAS  PubMed  Google Scholar 

  7. D. C. Hodgkin, J. Kamper, M. Mackay, J. Pickworth, K. N. Trueblood and J. G., White, Structure of Vitamin B12Nature 1956 178 64–66

    Article  CAS  Google Scholar 

  8. J., Halpern, Mechanism of coenzyme B12-dependent rearrangements Science 1985 227 869–875

    Article  CAS  Google Scholar 

  9. M. L. Ludwig and R. G., Matthews, Structure-based perspectives on B12-dependent enzymes Annu. Rev. Biochem. 1997 66 269–313

    Article  CAS  Google Scholar 

  10. R. Banerjee and S. W., Ragsdale, The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes Annu. Rev. Biochem. 2003 72 209–247

    Article  CAS  Google Scholar 

  11. H. G. Trüper and N. Pfennig, The family Chlorobiaceae, in The Prokaryotes, ed. A. Balows, H. G. Trüper, M. Dworkin, W. Harder and K.-H. Robin, Springer-Verlag, New York, 1992, p. 3583

    Google Scholar 

  12. T. M. Wahlund, C. R. Woese, R. W. Castenholz and M. T., Madigan, A thermophilic green sulfur bacterium from New Zealand hot springs, Chlorobium tepidum sp. nov. Arch. Microbiol. 1991 156 81–90

    Article  CAS  Google Scholar 

  13. J. A. Eisen, K. E. Nelson, I. T. Paulsen, J. F. Heidelberg, M. Wu, R. J. Dodson, R. Deboy, M. L. Gwinn, W. C. Nelson, D. H. Haft, E. K. Hickey, J. D. Peterson, A. S. Durkin, J. L. Kolonay, F. Yang, I. Holt, L. A. Umayam, T. Mason, M. Brenner, T. P. Shea, D. Parksey, W. C. Nierman, T. V. Feldblyum, C. L. Hansen, M. B. Craven, D. Radune, J. Vamathevan, H. Khouri, O. White, T. M. Gruber, K. A. Ketchum, J. C. Venter, H. Tettelin, D. A. Bryant and C. M., Fraser, The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium Proc. Natl. Acad. Sci. USA 2002 99 9509–9514

    Article  CAS  Google Scholar 

  14. N.-U. Frigaard, A. G. M. Chew, H. Li, J. A. Maresca and D. A., Bryant, Chlorobium tepidum: insights into the structure, physiology, and metabolism of a green sulfur bacterium derived from the complete genome sequence Photosynth. Res. 2003 78 93–117

    Article  CAS  Google Scholar 

  15. C. A. Roessner, M. J. Warren, P. J. Santander, B. P. Atshaves, S.-I. Ozaki, N. J. Stolowich, K. Iida and A. I., Scott, Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis FEBS Lett. 1992 301 73–78

    Article  CAS  Google Scholar 

  16. P. Spencer, N. J. Stolowich, L. W. Sumner and A. I., Scott, Definition of the redox states of cobalt-precorrinoids: investigation of the substrate and redox specificity of CbiL from Salmonella typhimurium Biochemistry 1998 37 14917–14927

    Article  CAS  Google Scholar 

  17. A. I., Scott, Reflections on the discovery of nature’s pathways to vitamin B12Chem. Rec. 2001 1 212–227

    Article  CAS  Google Scholar 

  18. S. Frank, A. A. Brindley, E. Deery, P. Heathcote, A. D. Lawrence, H. K. Leech, R. W. Pickersgill and M. J., Warren, Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway Biochem. Soc. Trans. 2005 33 811–814

    Article  CAS  Google Scholar 

  19. C. M. Borrego, L. J. Garcia-Gil Rearrangement of light harvesting bacteriochlorophyll homologues as a response of green sulfur bacteria to low light intensities Photosynth. Res. 1995 45 2–30

    Article  Google Scholar 

  20. C. M. Borrego, P. A. Gerola, M. Miller and R. P., Cox, Light intensity effects on pigment composition and organisation in the green sulfur bacterium Chlorobium tepidum Photosynth. Res. 1999 59 159–166

    Article  CAS  Google Scholar 

  21. R. Guyoneaud, C. M. Borrego, A. Mertinez-Planells, E. T. Buitenhuis, L. J. Garcia-Gil Light responses in the green sulfur bacterium Prosthecochloris aestuarii: changes in prosthecae length, ultrastructure, and antenna pigment composition Arch. Microbiol. 2001 176 278–284

    Article  CAS  Google Scholar 

  22. Y. Saga, S. Osumi, H. Higuchi and H., Tamiaki, Bacteriochlorophyll-c homolog composition in green sulfur photosynthetic bacterium Chlorobium vibrioforme dependent on the concentration of sodium sulfide in liquid cultures Photosynth. Res. 2005 86 123–130

    Article  CAS  Google Scholar 

  23. S. Fuhrmann, J. Overmann, N. Pfennig and U., Fischer, Influence of vitamin B12 and light on the formation of chlorosomes in green- and brown-colored Chlorobium species Arch. Microbiol. 1993 160 193–198

    CAS  Google Scholar 

  24. T. M. Wahlund and M. T., Madigan, Genetic transfer by conjugation in the thermophilic green sulfur bacterium Chlorobium tepidum J. Bacteriol. 1995 177 2583–2588

    Article  CAS  Google Scholar 

  25. Y. Saga, H. Oh-oka, T. Hayashi and H., Tamiaki, Presence of exclusively bacteriochlorophyll-c containing substrain in the culture of green sulfur photosynthetic bacterium Chlorobium vibrioforme strain NCIB 8327 producing bacteriochlorophyll-d Anal. Sci. 2003 19 1575–1579

    Article  CAS  Google Scholar 

  26. N.-U. Frigaard and D. A., Bryant, Chromosomal gene inactivation in the green sulfur bacterium Chlorobium tepidum by natural transformation Appl. Environ. Microbiol. 2001 67 2538–2544

    Article  CAS  Google Scholar 

  27. N.-U. Frigaard, Y. Sakuragi and D. A., Bryant, Gene inactivation in the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation Methods Mol. Biol. 2004 274 325–340

    CAS  PubMed  Google Scholar 

  28. H. D., Schweizer, Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis Biotechniques 1993 15 831–834

    CAS  PubMed  Google Scholar 

  29. Y. Saga, T. Wazawa, T. Mizoguchi, Y. Ishii, T. Yanagida and H., Tamiaki, Spectral heterogeneity in single light-harvesting chlorosomes from green sulfur photosynthetic bacterium Chlorobium tepidum Photochem. Photobiol. 2002 75 433–436

    Article  CAS  Google Scholar 

  30. Y. Saga and H., Tamiaki, Comparison between chlorosomes containing bacteriochlorophyll-c and chlorosomes containing bacteriochlorophyll-d isolated from two substrains of green sulfur photosynthetic bacterium Chlorobium vibrioforme NCIB 8327 J. Photochem. Photobiol., B 2004 75 89–97

    Article  CAS  Google Scholar 

  31. K. Wada, H. Yamaguchi, J. Harada, K. Niimi, S. Osumi, Y. Saga, H. Oh-oka, H. Tamiaki and K., Fukuyama, Crystal structures of BchU, a methyltransferase involved in bacteriochlorophyll c biosynthesis, and its complex with S-adenosylhomocysteine: implications for reaction mechanism J. Mol. Biol. 2006 360 839–849

    Article  CAS  Google Scholar 

  32. T. Nozawa, K. Ohtomo, N. Takeshita, Y. Morishita, M. Osawa and M. T., Madigan, Substituent effects on the aggregation of bacteriochlorophyll d homologues purified from Chlorobium limicola Bull. Chem. Soc. Jpn. 1992 65 3493–3494

    Article  CAS  Google Scholar 

  33. K. Uehara, M. Mimuro, Y. Ozaki and J. M., Olson, The formation and characterization of the in vitro polymeric aggregates of bacteriochlorophyll c homologs from Chlorobium limicola in aqueous suspension in the presence of monogalactosyl diglyceride Photosynth. Res. 1994 41 235–243

    Article  CAS  Google Scholar 

  34. Y. Saga, K. Matsuura and H., Tamiaki, Spectroscopic studies on self-aggregation of bacteriochlorophyll-e in non-polar organic solvents: effects of stereoisomeric configuration at the 31-position and alkyl substituents at the 81-position Photochem. Photobiol. 2001 74 72–80

    Article  CAS  Google Scholar 

  35. T. Mizoguchi, Y. Saga and H., Tamiaki, Isolation and structure determination of a complete set of bacteriochlorophyll-d homologs and epimers from a green sulfur bacterium Chlorobium vibrioforme and their aggregation properties in hydrophobic solvents Photochem. Photobiol. Sci. 2002 1 780–787

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Engineering, Kinki University, Higashi-Osaka, Osaka, 577-8502, Japan

    Yoshitaka Saga, Hiromitsu Hattori, Kanako Kaihara & Yuki Hirai

  2. Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga, 525-8577, Japan

    Jiro Harada & Hitoshi Tamiaki

  3. Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan

    Hirozo Oh-Oka

Authors
  1. Yoshitaka Saga
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  2. Jiro Harada
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  3. Hiromitsu Hattori
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  4. Kanako Kaihara
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Correspondence to Yoshitaka Saga.

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This paper was published as part of the themed issue of contributions from the 7th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms held in Kyoto, December 2007.

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Saga, Y., Harada, J., Hattori, H. et al. Spectroscopic properties and bacteriochlorophyll c isomer composition of extramembranous light-harvesting complexes in the green sulfur photosynthetic bacterium Chlorobium tepidum and its CT0388-deleted mutant under vitamin B12-limited conditions. Photochem Photobiol Sci 7, 1210–1215 (2008). https://doi.org/10.1039/b802354a

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