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Heat-induced changes in photosystem I activity as measured with different electron donors in isolated spinach thylakoid membranes

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Abstract

Heat-induced changes in photosystem I ( PSI) have been studied in terms of rates of oxygen consumption using various donors (DCPIPH2, TMPDred and DADred), formation of photo-oxidized P700 and changes in Chl a fluorescence emission at 77 K. Linear heating of thylakoid membranes from 35 °C to 70 °C caused an enhancement in PSI-mediated electron transfer rates (DCPIPH2→MV) up to 55 °C. However, no change was observed in PSI rates when other electron donors were used (TMPDred and DADred). Similarly, Chl a fluorescence emission spectra at 77 K of heat-treated thylakoid membranes did not show any increase in peak at 735 nm, however, a significant decrease was observed as a function of temperature in the peaks at 685 and 694 nm. In DCMU-treated control thylakoid membranes maximum photo-oxidized P700 was generated at g = 2.0025. In heat-treated thylakoid membranes maximum intensity of photo-oxidized P700 signal was observed at ∼50–55 °C without DCMU treatment. The steady-state signal of the photo-oxidized P700 was studied in the presence of DCPIPH2 and TMPDred as electron donors in DCMU-treated control and in 50 °C treated thylakoid membranes. We present here the first of such comparative study of PSI activity in terms of the rates of oxygen consumption and re-reduction kinetics of photo-oxidized P700 in the presence of different electron donors. It appears that the formation of the P700+ signal in heat-treated thylakoid membranes is due to an inhibited electron supply from PSII and not due to spillover or antenna migration.

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Authors and Affiliations

  1. School of Life Sciences, Life Science Annexe Building, Devi Ahilya University, Khandwa Road, Indore, 452017, M.P., India

    Arjun Tiwari, Anjana Jajoo & Sudhakar Bharti

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  1. Arjun Tiwari
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  2. Anjana Jajoo
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  3. Sudhakar Bharti
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Correspondence to Anjana Jajoo.

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Tiwari, A., Jajoo, A. & Bharti, S. Heat-induced changes in photosystem I activity as measured with different electron donors in isolated spinach thylakoid membranes. Photochem Photobiol Sci 7, 485–491 (2008). https://doi.org/10.1039/b719335a

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