Abstract
An obligatory step in the complex life cycle of the malaria parasite is sporogony, which occurs during the oocyst stage in adult female Anopheles mosquitoes. Sporogony is metabolically demanding, and successful oocyst maturation is dependent on host lipids. In insects, lipid energy reserves are mobilized by adipokinetic hormones (AKHs). We hypothesized that Plasmodium falciparum infection activates Anopheles gambiae AKH signaling and lipid mobilization. We profiled the expression patterns of AKH pathway genes and AgAkh1 peptide levels in An. gambiae during starvation, after blood feeding, and following infection and observed a significant time-dependent up-regulation of AKH pathway genes and peptide levels during infection. Depletion of AgAkh1 and AgAkhR by RNAi reduced salivary gland sporozoite production, while synthetic AgAkh1 peptide supplementation rescued sporozoite numbers. Inoculation of uninfected female mosquitoes with supernatant from P. falciparum-infected midguts activated AKH signaling. Clearly, identifying the parasite molecules mediating AKH signaling in P. falciparum sporogony is paramount.
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Introduction
Malaria is an important vector-borne disease caused by Plasmodium parasites, with more than 400,000 deaths and nearly 200 million cases annually1. Anopheles mosquitoes transmit Plasmodium parasites from human to human via an infectious bite. The obligatory mosquito stages of the malaria parasite represent a critical step in the complex life cycle of this pathogen. When adult female Anopheles mosquitoes ingest sexual stage Plasmodium gametocytes during blood feeding, the fully mature gametocytes undergo gametogenesis within the mosquito midgut to generate male and female gametes that fuse to form zygotes2. The zygotes then mature into motile ookinetes that migrate from the bloodmeal bolus to the periphery, traversing the midgut epithelial cells to the basal compartment where they form oocysts3. Each oocyst develops over a period of 12 to 17 days, expanding and undergoing massive mitotic divisions and morphological differentiation (a process called sporogony). These oocysts eventually rupture, releasing thousands of sporozoites that invade the salivary glands and are transmitted to the next vertebrate host via mosquito saliva deposition and parasite inoculation in the skin during a subsequent bloodmeal3. Sporogony is metabolically demanding4, and the parasite relies on its host vector as a source for nutrients5. Studies have shown that mosquitoes with larger teneral reserves are able to support higher parasite loads and survive longer than those grown with lower teneral reserves (from poor larval diet conditions), clearly indicating that mosquito host resources directly influence vector competence6,7,8. The magnitude and viability of the salivary gland sporozoite load is of great interest in malaria epidemiology as it impacts the success of malaria parasite transmission in a community and is a critical factor in the production of whole sporozoite-based malaria vaccines9,10.
Several studies have explored mosquito immune responses and nutrient allocation during the early stages of Plasmodium infection11,12,13,14,15. However, the underlying mechanism driving resource mobilization during sporogonic development remains poorly understood. It has been theorized that the malaria parasite has co-evolved with its Anopheles mosquito vectors to ensure maximum transmission potential for the parasite while minimizing vector fitness costs. Various studies have suggested potential parasite-driven manipulation of vector nutritional reserves and immune pathways in a developmental, stage-dependent manner to facilitate sporogony and enhance transmission potential11,16,17,18. Emerging evidence suggests that the malaria parasite taps into lipid reserves in the mosquito fat body for both its energy and structural needs5,11,12,13,19. Atella et al.13 described sequestration of the lipid transport protein, lipophorin, by oocyst stages of the avian malaria parasite Plasmodium gallinaceum in Aedes aegypti midguts ex vivo. Depletion of lipophorin has been shown to negatively impact the number and size of developing oocysts, the number of salivary gland sporozoites, sporozoite mitochondrial activity, and their mammalian infectivity11,12,18,20. A study by Costa et al.11 also showed that lipids are taken up by developing P. berghei oocysts in An. gambiae. Nutrient allocation in Plasmodium-infected An. gambiae has been suggested to be directed towards egg development at early timepoints following bloodmeal ingestion, with the parasite using the remaining non-egg shuttled nutrients in non-competitive vector-parasite interactions5,11,12. Together, these studies emphasize the central role of vector host stored lipids in Plasmodium sporogony.
Hormonal involvement has also been implicated in these stored lipid parasite-vector interactions. Depletion of 20-hydroxyecdysone (20E), which is an ecdysteroid hormone that controls metamorphosis, was shown to increase the expression of lipophorin, causing a subsequent accumulation of lipids in the midgut tissues and accelerating P. falciparum sporogony12. Through a non-competitive process, 20E has also been suggested to regulate nutrient allocation between mosquito reproduction and Plasmodium parasite development immediately following an infectious bloodmeal12. At the molecular level, nutrient allocation between developing mosquito eggs and parasite development is regulated by microRNAs, with miR-276 acting as a molecular switch in nutrient allocation by fine-tuning amino acid catabolism5. Insulin-like peptides (ILPs), which are involved in the regulation of hemolymph nutrient homeostasis21, have also been linked to a reduction in the An. stephensi innate immune response to P. berghei or P. falciparum infection14,15.
To gain more insight into Anopheles neuropeptide-mediated mobilization of stored fuels on P. falciparum sporogony, we investigated the role that mosquito adipokinetic hormones (AKHs) play in lipid-related resource allocation and lipid metabolism. AKHs are neuropeptide hormones that mobilize stored lipids and glycogen from the insect fat body during extended flight activity, starvation, pupation, and diapause22. AKHs belong to a family of short neuropeptides that consist of 7-10 amino acids with a conserved pyro-glutamate at the amino-terminus, two aromatic amino acid residues at position 4 (typically phenylalanine) and position 8 (tryptophan), and an amidated carboxy-terminus23,24. In An. gambiae two isoforms of AKHs are present, AgAkh1 (pQLFTPAWa) and AgAkh2 (pQVTFSRDWNAa)23. They are synthesized as prohormones and stored in the corpora cardiaca until needed25. Prior to secretion, the pro-protein is enzymatically cleaved to a single 8–10 amino acid peptide followed by modification at the amino terminus to pyroglutamic acid and amidation of the carboxy terminus26. Both peptides signal through a G protein-coupled receptor protein, AgAkhR23,27. Although the AKH pathway is known to mobilize stored lipids, it has not been studied in the context of An. gambiae – P. falciparum interactions. We hypothesized that P. falciparum infection influences An. gambiae AKH signaling, lipid metabolism, and mobilization of energy reserves to support the parasite’s metabolic and structural needs. Here we show that AKH signaling pathway transcripts and peptides are differentially expressed in a time-dependent manner during P. falciparum sporogony. We then employed RNAi to knockdown the AgAkh1 transcript, and exogenously injected synthetic AgAkh1 peptide to tease out the role of the mosquito AKH pathway in P. falciparum sporogony. We further demonstrated the activation of the AKH signaling pathway in uninfected mosquitoes following inoculation of ex vivo supernatant from P. falciparum oocyst-infected midguts.
Results
Starvation and blood feeding induces time-dependent AKH signaling in An. gambiae
Lipid mobilization in insects is known to be mediated by AKHs, which activates triglyceride lipase (TGL) through cAMP-dependent protein kinase A (PKA, Fig. 1a). PKA also phosphorylates lipid-associated proteins such as lipid storage droplet protein 128 (Lsdp1, Fig. 1a). To establish a baseline for the regulation of AKH signaling in malaria vectors, we subjected female An. gambiae (Keele strain) to varying lengths of starvation (Fig. 1b (i)) and quantified five transcripts (AgAkh1, AgAkh2, AgAkhR, AgLsdp1, and lipophorin (AgLp)) in the AKH pathway after each period of starvation (in comparison to their sugar-fed counterparts). The three time points (i.e., 3, 6, and 24 h of starvation) were selected to capture different levels of energy mobilization within a short time frame. AgLsdp1 was chosen as a proxy for lipid reserve mobilization based on previous studies showing it plays a key role in determining the rate of AKH-mediated lipolysis in several insects, such as Manduca sexta29, while AgLp was chosen to detect lipid trafficking from the fat body30. The experimental approach is summarized in Fig. 2a.
As expected, a significant upregulation of AgAkh1, AgAkh2, and AgAkhR transcripts were detected during starvation (Fig. 2b and Supplementary Fig. S1a); with starved females expressing greater than 50-fold more AgAkh1 transcripts than their sugar-fed counterparts after 3 h of starvation across the three replicates (W = 81, P < 0.001; Fig. 2b and Supplementary Fig. S1A). Variable fold-change was observed in the expression of AgAkh1, AgAkh2, AgAkhR, AgLsdp1, and AgLp between starved and sugar-fed mosquitoes in the three replicates at different time points (Fig. 2b and Supplementary Fig. S1a). The activation of AKH signaling was further corroborated by an AgAkh1 peptide enzyme-linked immunosorbent assay (peptide ELISA). We observed a significant increase in AgAkh1 peptide levels after 3 h (5.1-fold; t = 4.61, df = 5, P = 0.003) and 24 h (516-fold; t = 48.56, df = 5, P < 0.001) of starvation (Fig. 2c). Similar increases in peptide levels after 3 and 6 h of starvation were detected in the second and third replicates, although variation in the level of peptide expression was noted across the replicates, owing to the difficulty in controlling for variation in teneral reserves of individual mosquitoes in a pool (Supplementary Fig. S1b). In insects, AKH activity has been shown to be antagonized by the insulin/insulin-like growth factor (IIS) signaling pathway, which regulates the level of trehalose in hemolymph through the induction of lipogenesis31. AKH and IIS pathways communicate through liver kinase B1 (LKB1) – Salt-inducible kinase 3 (SIK3) pathways (Fig. 1a). In An. gambiae, seven insulin-like peptide (ILP) genes in the IIS pathway have been annotated32. However, ILP6 and ILP7 were shown to be duplicate genes of ILP3 (98.7% amino acid sequence identity) and ILP1 (95.5% amino acid sequence identity), respectively33, and as such, there are five ILPs including ILP1/7, ILP2, ILP3/6, ILP4, and ILP532,33. We profiled all five An. gambiae ILP transcripts and their receptor tyrosine kinase homodimer (AgInR; Fig. 1a). We observed a significant decrease in transcript levels of AgILP2 after 6 and 24 h (2.0-fold, t = 6.59, df = 17, P = 0.006; and 2.6-fold, W = 0, P < 0.001, respectively), AgILP3 after 3 h (1.7-fold, W = 0, P < 0.001) and 24 h (2.2-fold, W = 4, P = 0.014), and AgInR after 3 h (1.9-fold, W = 0, P = 0.004) of starvation (Fig. 2b and Supplementary Fig. S1a). AgILP3 transcripts were 5.7-fold higher in starved than sugar-fed mosquitoes after 6 h of starvation (W = 81, P < 0.001), while AgInR transcripts were 2-fold higher at 6 and 24 h of starvation (P = 0.004 and 0.005, respectively; Fig. 2b and Supplementary Fig. S1a). No difference was detected in the transcript levels of AgILP1, AgILP4, AgILP5, AgILP6 and AgILP7 (Supplementary Table S2).
To establish a baseline for the regulation of the AKH signaling pathway following a bloodmeal, we investigated the expression of the same transcripts as above (Supplementary Table S1) in non-infectious blood fed An. gambiae at 6-, 24- and 48 h post bloodmeal (hpbm) feeding (spanning the physiological window of blood feeding from full engorgement with blood until the bloodmeal is almost fully digested), with age-matched sugar-fed females as controls (Fig. 1b (ii)). There was a significant upregulation of AgAkh1 at 6 (1.9-fold, W = 36, P = 0.005), and 48 (5-fold, W = 36, P = 0.008) hpbm; AgAkh2 at 24 (1.5-fold, W = 32 P = 0.030), and 48 (3.9-fold, W = 36, P = 0.001) hpbm; AgAkhR at 48 hpbm (2.4-fold, P = 0.009); AgLsdp1 at 24 (2.4-fold, P = 0.019) and 48 hpbm (4.5-fold, W = 36, P = 0.005); and AgLp at 6 (19-fold, W = 36, P = 0.002) and 24 (7.9-fold, W = 36, P = 0.005) hpbm (Fig. 2d). Similar upregulation patterns in AKH pathway transcript levels were observed in replicates 2 and 3 (Supplementary Fig. S1c). The activation of AKH signaling was further confirmed by peptide ELISA, which revealed a significant increase in AgAkh1 peptide levels at 6 and 24 hpbm (>1,947-fold change, t = 46, df = 5, P < 0.001; t = 47.06, df = 5, P < 0.001, respectively) but not at 48 hpbm in blood-fed females, compared to sugar-fed females (Fig. 2e and Supplementary Fig. S1d). In the IIS pathway, only AgILP2 and AgInR transcripts were significantly upregulated at 24 (3.7-fold, W = 36, P = 0.006), and 48 (3.2-fold, t = 5.89, df = 11, P = 0.005) hpbm, respectively (Fig. 2d and Supplementary Fig. S1c). Akin to what we observed for expression patterns in starved mosquitoes, no significant difference was detected in the transcript levels of AgILP1, AgILP4, AgILP5, AgILP6 and AgILP7 (Supplementary Table S2).
Plasmodium falciparum infection induces AKH signaling in An. gambiae
To understand how malaria parasite sporogony affects AKH signaling, we infected female An. gambiae (Keele strain) with P. falciparum (NF54 strain) and sampled at 1-, 2-, 3-, 4-, 5-, and 8-days postinfectious bloodmeal (dpib) to quantify AKH pathway transcripts (Fig. 1b (iii)). The time points were chosen to capture early stages of midgut invasion by the P. falciparum ookinetes through mid-to-late stages of oocyst development. Controls, dissected at the same time points, included age-matched females fed a non-infectious bloodmeal. A subset of mosquitoes was dissected from each replicate at 8 dpib to determine oocyst intensity and prevalence as a proxy for infection success. A high oocyst prevalence (86%, 95%, and 90%) and high oocyst intensity (median number of oocysts per midgut = 35, 55.5, and 67) were observed in three replicate experiments. There was a significant increase in the expression of AgAkh1 (2.3-fold, t = 5.83, df = 7, P = 0.009; 2.1-fold, t = 5.12, df = 7, P = 0.001, respectively), AgAkh2 (2.6-fold, t = 6.16, df = 7, P < 0.001; 2.3-fold, t = 3.54, df = 7, P = 0.004, respectively), and AgAkhR (2.1-fold, t = 3.56, df = 7, P = 0.019; 2.9-fold, t = 4.36, df = 7, P = 0.001, respectively) in P. falciparum-infectious bloodfed mosquitoes compared to non-infected females at 3 and 4 dpib (Fig. 2f and Supplementary Fig. S1E). In addition, AgLsdp1 transcripts were significantly increased at 4 dpi (2.8-fold, t = 2.85, df 7, P = 0.004), while AgLp transcripts were significantly increased at 3 and 4 dpib (2.6-fold, t = 2.79, df = 7, P = 0.019; 4.1-fold, t = 13.29, df = 7, P = 0.002, respectively), compared to uninfected females (Fig. 2f and Supplementary Fig. S1e). No difference in AgAkh1 peptide levels were observed between mosquitoes fed on uninfected and P. falciparum-infected blood at 1 and 2 dpib across the three replicates (Fig. 2g and Supplementary Fig. S1f). However, AgAkh1 peptide levels were significantly elevated in P. falciparum infectious bloodfed An. gambiae 3 dpib (1.3-fold; t = 2.574, df = 11, P = 0.030; Cohen’s d = 5.423), 4 dpib (1.3-fold; t = 5.467, df = 11, P = 0.002; Cohen’s d = 6.856), 5 dpib (3.6-fold; t = 7.246, df = 11, P < 0.001; Cohen’s d = 10.175), and 8 dpib (1.8-fold; t = 9.580, df = 11, P < 0.001; Cohen’s d = 19.174) compared to uninfected An. gambiae in replicate 1 (Fig. 2c). A significant increase in AgAkh1 peptide levels was also detected in P. falciparum-infected mosquitoes at 3-, 4-, and 5 dpib in replicate 3 and at 8 dpib in both replicates 2 and 3 (Supplementary Fig. S1f). A significant increase in transcript levels in P. falciparum-infectious bloodfed mosquitoes were also observed for AgILP2 at 8 dpib (2.9-fold, t = 8.02, df = 7, P = 0.003); AgILP3 at 3, 4, and 8 dpib (4.6-fold, W = 16, P = 0.017; 1.6-fold, t = 2.83, df = 7, P = 0.040; 3.2-fold, t = 4.62, df = 7, P = 0.016, respectively); and AgInR at 4 dpib (3.4-fold, t = 4.81, df = 7, P = 0.007), compared to uninfected blood fed mosquitoes (Fig. 2f and Supplementary Fig. S1e). Variable fold change in gene expression of AgILP1, AgILP4, AgILP5, AgILP6, and AgILP7 between infected and uninfected An. gambiae at different time points was also observed (Supplementary Table S2).
Adipokinetic hormone signaling promotes P. falciparum sporogony in An. gambiae
To establish the role of AKH signaling in P. falciparum sporogony, we used RNA interference (RNAi) to reduce AKH transcript levels (Fig. 3a). First, we attempted knockdown of AgAkh1, AgAkh2 and AgAkhR transcripts in uninfected females through intrathoracic injection of corresponding double-stranded RNAs (dsRNA). Whereas knockdown of AgAkh2 was unsuccessful, we obtained > 90% knockdown of AgAkh1 (W = 0, P < 0.001) and 73% knockdown of AgAkhR (W = 0, P < 0.001) transcripts compared to dsGFP (control)-treated mosquitoes (Fig. 3b). Injection of mosquitoes with a mixture of dsAgAkh1 and dsAgAkhR depleted AgAkh1 transcripts by 78% (W = 0, P < 0.001, Fig. 3b) and AgAkhR by 74% (W = 0, P < 0.001, Fig. 3b). We further evaluated the duration of dsAgAkh1, dsAgAkhR, and dsAgAkh1 + dsAgAkhR activity by quantifying transcript levels at 1-, 3-, and 5-days post dsRNA treatment in non-infected mosquitoes. AgAkh1 transcript levels were significantly reduced during time points that would correspond to early oocyst development at day 1 (92%, W = 0, P < 0.001, Fig. 3c) and day 3 (87%, W = 0, P < 0.001, Fig. 3c) and mid-oocyst development at day 5 (61%, t = −10.64, df = 7, P < 0.001, Fig. 3c) post dsAgAkh1 treatment, as compared to dsGFP controls, suggesting that the duration of knockdown is sufficient to study the impact of AgAkh1 on Plasmodium oocyst development (Fig. 3c). Similar patterns in depletion of AgAkh1 transcripts at days 1 (77%, W = 0, P = 0.002), 3 (78%, t = −9.71, df = 11, P < 0.001) and 5 (88%, W = 0, P = 0.002) post dsRNA treatment was observed in mosquitoes injected with dsAgAkh1 + dsAgAkhR (Fig. 3c). However, AgAkhR transcripts was only significantly depleted on day 1 after treatment with dsAgAkh1, dsAgAkhR and dsAgAkh1 + dsAgAkhR (t = −7.07, df = 11, P < 0.001) (Fig. 3d). No significant difference was detected in the AgAkhR transcript on day 3 and 5 (Fig. 3d). Treatment with dsAgAkh1 and dsAgAkh1 + dsAgAkhR significantly reduced the transcript levels of AgLsdp1 (t = 3.74, df = 11, P = 0.005 and t = 4.16, df = 11, P = 0.002, respectively) 1 day post injection compared to dsGFP treated mosquitoes (Table 1). Treatment with dsAgAkh1 + dsAgAkhR also significantly reduced the transcript levels of AgLsdp1 (t = 4.20, df = 11, P = 0.002) 5 days post injection compared to dsGFP treated mosquitoes (Table 1). No significant difference was detected in AgLp transcript levels between the dsRNA treatments on day 1, 3 and 5 (Table 1).
To disrupt AKH regulation during the sporogonic stages of the malaria parasite, we injected female An. gambiae with dsAgAkh1, dsAgAkh1 + dsAgAkhR or dsGFP three days postinfectious bloodmeal. Since treatment with dsAgAkhR alone did not significantly reduce the AgAkhR transcript levels beyond day 1 post-treatment, its effect on P. falciparum sporogony was not evaluated further. We confirmed knockdown efficiency by quantifying AgAkh1 and AgAkhR transcripts and validating the observed reduction at the peptide level at 24 h post dsRNA injection. We detected a knockdown of AgAkh1 (95%, W = 0, P < 0.001; and 95% W = 0, P < 0.001) and AgAkhR (35%, t = 6.24, df = 17, P = 0.001; and 80%, W = 0, P < 0.001) transcripts following treatment with dsAgAkh1 and dsAgAkh1 + dsAgAkhR, respectively (Fig. 3e), compared to the dsGFP-treated group. The AgAkh1 peptide levels were also significantly reduced in dsAgAkh1 (W = 0, P < 0.001; Fig. 3f) -treated mosquitoes compared to the dsGFP-treated group. The oocyst prevalence was 74%, 85%, and 91% for dsGFP, dsAgAkh1, and dsAgAkh1 + dsAgAkhR treated mosquitoes 8 dpib, respectively (Table 2). No significant difference was detected in oocyst intensity or prevalence across the three dsRNA treatments (χ2(2, 102) = 1.710, P = 0.425; χ2 = 4.112, df = 2, P = 0.128; Table 2). RNAi treatment with dsAgAkh1 treatment resulted in 1.2-fold reduction in oocyst diameter (Z = −1.863, P = 0.082, Cohen’s d = 0.478, Fig. 3h) while dsAgAkh1 + dsAgAkhR treatment reduced oocyst diameter by 1.2-fold (Z = −2.420, P = 0.047, Cohen’s d = 0.655, Fig. 3h). Similarly, treatment with dsAgAkh1 and dsAgAkh1 + dsAgAkhR resulted in significant reduction in salivary gland sporozoite infection intensity. Treatment with dsAgAkh1 reduced the sporozoite intensity by 70% (Z = − 3.485, P = 0.001; Cohen’s d = 0.502, Fig. 3i) at 14 dpib while treatment with dsAgAkh1 + dsAgAkhR reduced the salivary gland sporozoite intensity by 65% (Z = −2.951, P = 0.008, Cohen’s d = 0.430, Fig. 3i). The sporozoite prevalence for dsGFP, dsAgAkh1, and dsAgAkh1 + dsAgAkhR treatment was 83%, 80%, and 83%, respectively. There was no significant difference in sporozoite infection prevalence between dsAgAkh1-, dsAgAkh1 + dsAgAkhR-, and dsGFP-treated mosquitoes (χ2 = 0.589, df = 2, p value = 0.745).
Recombinant adipokinetic peptide hormone increases P. falciparum sporozoite infection intensity in An. gambiae salivary glands
To provide further orthogonal evidence for the role of AKH signaling in P. falciparum sporogony, we injected synthetic AgAkh1 (SAgAkh1) and AgAkh2 (SAgAkh2) peptides into mosquitoes (Fig. 4a). First, we tested the potential of these synthetic peptides to mimic natural peptides by micro-injecting sugar-fed female An. gambiae with SAgAkh1 and SAgAkh2 peptides and evaluating their effect on the downstream genes AgAkhR, AgLsdp1, and AgLp. Inoculation of SAgAkh1 did not elicit a significant change in AgAkhR transcripts (t = 1.82, df = 11, P = 0.191, Fig. 4b), with only SAgAkh1 inducing a significant upregulation of AgLsdp1 (2.2-fold, W = 0, P = 0.049, Fig. 4b) and AgLp (2.8-fold, W = 0, P = 0.017, Fig. 4b) three hours post-injection compared to sterile PBS-injected controls; like the patterns observed in starved versus sugar-fed females. Next, we micro-injected P. falciparum-infectious blood-fed female mosquitoes with SAgAkh1 and SAgAkh2 peptides at 3 dpib and 5 dpib. Oocyst diameter and salivary gland sporozoite infection intensity was used to gauge the impact of the synthetic peptides on sporogonic development, while oocyst prevalence and intensity were used as a proxy for successful infection.
Like the RNAi studies, no significant difference was detected in 8 dpib oocyst intensity or prevalence when mosquitoes were injected with both peptides at 3 dpib (χ2(2, 106) = 1.906 P = 0.386; χ2 = 2.814, df = 2, P = 0.245, respectively, Table 2) and 5 dpib (χ2(2, 102) = 0.793, P = 0.0.673;; χ2 = 0.423, df = 2, P = 0.809, respectively, Table 2). Mosquitoes injected with SAgAkh1 at 3 and 5 dpib had 1.2-fold (Z = −4.045, P < 0.001; Cohen’s d = 1.340) and 1.1-fold (Z = −2.598; P = 0.024; Cohen’s d = 0.342) larger oocyst diameters compared to the sterile PBS (control)-treated group, respectively (Fig. 4c). Treatment with SAgAkh1 peptide at 3 dpib resulted in a 165% increase in salivary gland sporozoite numbers (Z = −3.579, P = 0.001; Cohen’s d = 0.969; Fig. 4d). Treatment with SAgAkh2 at 3 dpib led to a 1.2-fold (Z = 1.254, P = 0.004; Cohen’s d = 1.192) increase in oocyst diameter (Fig. 4c) but did not result in a significant increase in salivary gland sporozoite numbers (Fig. 4d). The 14 dpib sporozoite prevalence for PBS-, SAgAkh1-, and SAgAkh2-treated mosquitoes was 79%, 83% and 78%, respectively, 3 dpib, and 88%, 91% and 87%, 5 dpib, and these results were not significantly different (X-squared = 0.39745, df = 2, p value = 0.8198, 3dpib, X-squared = 0.6273, df = 2, p value = 0.7308, 5dpib).
Plasmodium falciparum oocyst supernatant stimulates AKH signaling
The blood stages of Plasmodium parasites have been estimated to export more than 400 proteins (known as the secretome or exportome) into the host erythrocytes or plasma that can modulate host cell physiology and immune response34,35,36,37. We posited that the rapidly developing oocyst stages of the parasite secrete proteins into the mosquito hemolymph that modulate lipid reserve mobilization. To test this, we dissected midguts from mosquitoes fed on P. falciparum-infectious blood at 3-, 4-, and 5 dpib and incubated them overnight in incomplete medium, which corresponds to the different oocyst developmental stages and our previously observed differences in transcript profiles. The midgut supernatants were collected after a 24 h incubation, concentrated, and injected into uninfected 5- to 6-day-old females. Day 8 median oocyst intensity and prevalence were 8.5 oocysts/midgut and 90%, 39 oocysts/midgut and 90%, and 60 oocysts/midgut and 90% for replicates 1, 2, and 3, respectively. Midgut supernatant from infectious blood-fed mosquitoes from 3 dpib did not elicit any change in the expression levels of AgAkh1, AgAkh2, AgAkhR, AgLsdp1, and AgLp transcripts, while the expression levels of AgAkh2 and AgLp were downregulated 1.7-fold (t = 2.49, df 11, P = 0.035) and 4.6-fold (t = −6.34, df = 11, P < 0.001), respectively, in mosquitoes injected with day 5 dpib midgut supernatant from infectious blood-fed mosquitoes (Fig. 4e). On the other hand, 4 dpib midgut supernatant from infectious blood-fed mosquitoes elicited significant upregulation of AgAkh1 (8.5-fold, W = 36, P = 0.001), AgAkh2 (2.7-fold, t = 6.35, df = 11, P < 0.001), and AgLp (3.6-fold, W = 36, P = 0.002) transcripts (Fig. 4e). The observed activation of AKH pathway genes by 4 dpib with midgut supernatant from infectious blood-fed mosquitoes suggested that signaling to mobilize lipid reserves occurred due to an increased metabolic demand imposed by the rapidly growing parasites.
To test this, we repeated the previous study but only incubated 4 dpib P. falciparum-infected midguts in two sets of media, RPMI only and RPMI plus heat-inactivated fetal bovine serum (FBS). Midguts of age-matched females fed on a non-infectious bloodmeal and collected at the same time intervals, as well as RPMI + FBS with no midguts were used as controls. Day 8 median oocyst intensity and prevalence were 60.5 oocysts/midgut and 100%, 44.5 oocysts/midgut and 80%, and 139 oocysts/midgut and 90% for replicates 1, 2 and 3, respectively. As expected, there was no significant difference in the expression levels of AgAkh1, AgAkh2, AgAkhR, AgLsdp1, and AgLp when lipid-rich FBS was added, as compared to non-infectious blood-fed controls (Fig. 4f). Injection of female mosquitoes with supernatant from 4 dpib midguts infectious blood-fed mosquitoes without FBS resulted in a significant upregulation of AgAkh1 (3.2-fold, t = 2.64, df = 11, P = 0.029), AgAkh2 (1.7-fold, 4.89, df = 11, P = 0.001), and AgLsdp1 (2-fold, t = 3.48, df = 11, P < 0.001) compared to those inoculated with supernatant from uninfected midguts (Fig. 4f). A significant difference in AgAkh1 peptide concentration was detected between mosquitoes treated with RPMI media and 4 dpib midgut supernatant from infectious blood-fed mosquitoes (W = 147.5, P = 0.004; Cohen’s d = 0.515), but not between those treated with uninfected and infected midgut supernatants (W = 228.5, P = 0.068, Cohen’s d = 0.202) (Fig. 4g).
Discussion
The role of AKH signaling is poorly understood in the malaria vector An. gambiae. Using starvation and blood-feeding assays, we explored the role of the AKH signaling pathway in this mosquito species and its involvement in lipid metabolism and utilization of energy reserves (Fig. 5). During starvation, AKHs mobilize glycogen and lipids from the fat body of several insect species to sustain metabolic processes vital for survival38,39,40. AKHs have been shown to induce lipolysis in the fat body of Manduca sexta and Drosophila melanogaster through PKA-mediated phosphorylation of Lsdp1, thus allowing triglycerides (TAG) to access the hydrophobic lipid droplet surface and hydrolyze TAG to diglycerides and free fatty acids28,29,41. Our study shows an upregulation of AKH pathway genes including AgAkh1, AgAkh2, AgAkhR, and AgLsdp1 in starved female mosquitoes within a 48 h period. AgLp, which is involved in trafficking of mobilized lipids, was also upregulated. In addition, AgAkh1 peptide levels were significantly elevated, pointing to the possible involvement of this pathway in energy reserve mobilization. On the other hand, blood-feeding induces a massive metabolic shift geared towards egg production that is accompanied by mobilization and deposition of lipids in developing oocytes30. Our study shows a time-dependent increase in the transcript levels of the AKH pathway genes over 48 h post blood feeding; data that are congruent with previous findings by Kaufmann and Brown23 describing the expression of AgAkh1, AgAkh2, and AgAkhR transcripts in different tissues of blood-fed female An. gambiae. The robust expression of AgAkh1 peptide within 24 h post blood feeding indicates a relatively rapid and sustained process, putatively involving enzymatic cleavage of the prohormone triggered by a bloodmeal signal. Our results also revealed time-based downregulation of AgILP2, AgILP3, and AgInR during starvation and upregulation following a bloodmeal, suggesting active involvement of the IIS pathway in hemolymph nutrient homeostasis in the malaria vectors31.
Plasmodium parasites require nutrients for metabolic processes that support both parasite schizogony (human) and sporogony (mosquito). Whereas nutrient utilization has been extensively studied in the blood stages of the parasite42, nutrient utilization during sporogony is poorly understood. 20E and microRNAs have been shown to regulate nutrient allocation between developing eggs and early stages of malaria parasite development in the mosquito following an infectious bloodmeal5,12. Here, we demonstrate the activation of mosquito AKH signaling during P. falciparum sporogony between day 3 and 8 postinfectious bloodmeal (Fig. 5). These findings underpin the significance of these time points in the biology of Plasmodium sporogony. The parasite completes its transition into oocysts within 36 hours of ingestion by Anopheles, and initiates the process of sporoblast formation, extensive DNA replication, cell division, and maturation resulting in the production of sporozoites3. Unlike the asexual blood stages that rely on glycolysis, the mosquito stages of Plasmodium are dependent on the tricarboxylic acid (TCA) cycle, with one input being acetyl-CoA derived from catabolism of free fatty acids43. The increased AKH pathway signaling between day 3 and 4 postinfection is, in part, suggestive of the response in the mosquito vector to increased demand of lipids for energy and structural components such as cell membranes by the oocyst. We observed an initial spike in AgAkh1 peptide levels in females fed a non-infectious and infectious bloodmeal within the first 24 hours, in support of the logical notion that mobilization of stored lipids at this time is to support egg development. AgAkh1 peptide levels begin to rise again after 3 dpib, becoming significantly different from the uninfected group at 3-, 4-, 5-, and 8 dpib, suggesting that the developing parasite can influence AKH signaling during sporogony, long after blood feeding and blood digestion. These data suggest that even if the mosquito vector is not able to take another bloodmeal, the maturing oocyst has evolved a mechanism to trigger lipid mobilization.
Studies by Werling et al.12 demonstrated increased accumulation of glycerolipids and phospholipids in the midguts of nuclear receptor EcR/USP depleted An. gambiae, which is associated with accelerated P. falciparum development. Although this was attributed to possible downregulation of An. gambiae hepatocyte nuclear factor 4 that controls lipolytic genes12, the involvement of other pathways was not ruled out. We demonstrate the involvement of An. gambiae AKH signaling in the regulation of P. falciparum sporogony. Specifically, depletion of AgAkh1 and AgAkhR transcripts through RNAi resulted in a decrease in oocyst diameter and lower salivary gland sporozoite infection intensity, suggesting an arrest in the development of oocysts. On the other hand, supplementing infected female An. gambiae with synthetic AgAkh1 peptide resulted in larger oocyst diameters and higher sporozoite numbers in the salivary glands. Previous studies have shown that giving mosquitoes a second bloodmeal a few days after the first infectious bloodmeal accelerates the rate of parasite development and total salivary gland sporozoite load, possibly due to additional nutrients acquired from the second bloodmeal44,45. Therefore, our findings indicate activation of AKH signaling pathway has the potential to increase sporozoite production even in the absence of multiple bloodmeals. Although we were unable to deplete AgAkh2 transcripts, the addition of synthetic AgAkh2 peptide did not elicit any change in parasite development or sporozoite infection intensity, suggesting its presumed non-involvement. Together, these data highlight a vector host-pathogen dynamic in the use of mosquito lipid energy reserves during sporogonic development, likely a result of a long evolutionary relationship between the malaria parasite and its primary vector (Fig. 5). While our study demonstrates that AKH signaling plays a significant role in P. falciparum sporogony, additional studies evaluating the effect of RNAi and synthetic peptide injections at additional time points in the parasite sporogony is necessary to tease out the kinetics of its involvement.
The blood stages of the malaria parasites have been shown to secrete a plethora of proteins into the host erythrocytes and plasma during development27,28. These proteins serve as virulence factors that can modulate or evade immune responses, as well as mobilize key resources to support parasite development. For example, P. falciparum-secreted rhoptry protein RhopH2 was shown to increase erythrocyte membrane permeability thus allowing uptake of nutrients46. In addition, P. falciparum Niemann type C like 1 (NPC1L1) protein has been shown to import sterols and maintain Plasmodium plasma membrane lipid homeostasis47. During sporogony, we show the activation of AKH pathway genes in uninfected mosquitoes following injection of supernatant from infected midguts, similar to patterns of activation of AKH pathway genes that were observed in infected mosquitoes. The fact that the AKH pathway genes were not activated when mosquitoes were injected with supernatant from midguts incubated in FBS-containing media further alludes to the central role played by lipids in parasite sporogony. This could have been caused by the fact that FBS has ample lipid content, hence the parasite did not need to stimulate lipid mobilization. In addition, the activation of the AKH pathway by supernatant from 4 dpib midguts is intriguing. It suggests that the window within which the parasite passively or actively triggers resource mobilization in mosquito vectors is small. This can be attributed to the fact that around this time, sporoblast formation is complete and the sporozoites are rapidly differentiating and forming circumsporozoite protein3.
Our data also suggest that lipid mobilization may be a result of heretofore uncharacterized interactions between mosquito tissues and parasite-secreted molecules, or increased lipid mobilization by mosquito in response to parasite infection and represents a significant step towards understanding the potential manipulation of mosquito physiology by the parasite. In the future, it will be important to determine if this interaction is active, i.e., the parasite secretes specific molecules to mobilize vector host lipid stores, or if the molecules (some of which may not be normally secreted) are passively exported or expelled as part of a remodeling process as one oocyst becomes thousands of sporozoites. In the latter scenario, the molecules are then “sensed” by the mosquito, signaling through the AKH pathway. Further studies will be needed to elucidate the identity and role of these molecules in parasite transmission biology. Furthermore, the current study cannot rule out the potential role of mosquito-secreted molecules in response to oocyst maturation.
Our study presents insights into P. falciparum sporogony and opportunities to increase sporozoite yield for whole sporozoite-based vaccine development and controlled human challenge studies, which are impacted by the challenge of routinely and cost-effectively producing large numbers of sporozoites at the requisite scale48. However, we anticipate that a downside of manipulating this system through the AKH pathway, is that the delicate balance of the natural system is perturbed, which may result in reductions in mosquito fitness and viability, i.e., requiring large numbers of mosquitoes or reducing adult mosquito output in the insectary to ensure large, robust females are produced. This study also lays the foundation for the development of new malaria control interventions, i.e., drugs that can clear a mosquito of oocyst infections or arrest the development of sporozoites within intact oocysts by modulating nutrient mobilization via AKH signaling49.
Methods
Mosquito colony maintenance
An. gambiae (Keele) was used in all experiments. Adults were reared at a temperature of 27 °C with a relative humidity of 75–80% and a 12-h light/dark photoperiod. Larvae were maintained on Koi fish food pellets and adults were offered 10% sugar solution ad libitum.
Starvation and blood feeding assays
Two separate feeding assays were carried out with An. gambiae mosquitoes. In the first assay, 5- to 6-day-old adult female mosquitoes with no prior bloodmeal were starved of sucrose solution for 3, 6, or 24 h. Controls included age-matched females drawn from the same mosquito cohort but given 10% sucrose solution ad libitum. At each time point, (3-, 6-, and 24 h) nine mosquitoes were sampled. Mosquitoes were either i) pooled into groups of three and deposited in 1.5 mL microcentrifuge tubes containing TRIzol reagent, and immediately stored at −80 °C (for RT-qPCR assays) or ii) pooled into groups of three, snap-frozen in liquid nitrogen and stored at −80 °C (for enzyme-linked immunosorbent assays (ELISAs)). In the second assay, 5- to 6-day-old adult female An. gambiae were starved for 6 h, then given either 10% sucrose solution (control) or a human bloodmeal (50% hematocrit in heat-inactivated human serum) offered through membrane feeding assays for 30 min. Similarly, nine females were sampled from each feeding condition at 6-, 24-, and 48 h post bloodmeal and pooled into groups of three. Again, mosquitoes were either stored in TRIzol reagent or snap-frozen in liquid nitrogen. For each feeding assay, we performed three independent biological replicate experiments.
Plasmodium falciparum infection assays
A culture of P. falciparum (NF54) gametocytes was produced as follows49. Gametocyte cultures were seeded at 0.5% asexual parasitemia and 4% human hematocrit and were grown under hypoxic conditions in RPMI culture media supplemented with HEPES, hypoxanthine, glutamine, and 10% heat-inactivated human serum. The RPMI media was changed daily during culturing and gametocytes were harvested at day 17. Infected erythrocytes were brought up in heat-inactivated human serum (Interstate Blood Bank, Memphis, TN) plus human RBCs (blood type O+) at 1% gametocytemia and 50% hematocrit. Infective blood was delivered directly into glass, water-jacketed membrane feeders warmed to 37 °C. Mosquitoes (6–8 day old) were allowed to feed for 20 min. Fully blood-engorged female mosquitoes were separated and maintained for 14 days on 10% sucrose solution. A sub-set of whole mosquitoes were collected at 1-, 2-, 3-, 4-, 5-, and 8-days postinfectious bloodmeal (dpib) and either preserved in TRIzol for RT-qPCR or snap frozen in liquid nitrogen for ELISA. A separate subset of mosquitoes was collected at 8 dpi for midgut oocyst enumeration. Controls included age-matched females fed on a non-infectious bloodmeal collected under the same sampling regime. At each sampling point, nine females were sampled and processed in pools of three. A total of three biological replicates were obtained for each time point analyzed. The dissected midguts were stained with 0.1% mercurochrome to visualize and quantify oocyst burden. For sporozoite quantification, salivary glands were dissected in 1X phosphate buffered saline (PBS) and transferred individually into 1.5 mL tubes containing 100 µL DNAzol.
Quantification of vector host gene expression profiles by quantitative PCR
Total RNA was extracted from the starved, sugar-fed, blood-fed, and P. falciparum-infected mosquito pools in TRIzol reagent (Invitrogen) according to the manufacturer’s instructions, including a DNase I (Turbo DNase kitTM, ThermoFisher) step. cDNA was synthesized from 1 µg of RNA using Superscript IV Reverse Transcriptase and Oligo(dTs) (ThermoFisher).
The expression of AKH pathway genes (AgAkh1, AgAkh2, AgAkhR, AgLsdp1, and AgLp) and insulin/insulin-like growth factor genes (AgILP1, AgILP2, AgILP3, AgILP4, AgILP5, AgILP6, AgILP7, and AgInR) were quantified from the synthesized cDNA via qPCR, using An. gambiae ribosomal protein transcripts AgRPL32 as internal controls. Primer pairs were designed using Integrated DNA Technology freeware (https://www.idtdna.com/scitools/Applications/RealTimePCR/) so that at least one oligonucleotide spanned an exon – exon boundary to prevent genomic DNA amplification. Amplicons were designed to be no longer than 150 base pairs, to promote amplification efficiency (Supplementary Table S1). Transcript sequence and exon boundary information were retrieved from VectorBase50. Primer specificity was first verified in silico by BLAST comparisons of the oligonucleotide sequences against all sequences hosted by National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/). Optimal amplifying and annealing conditions were then tested by gradient PCR for each set of primers. In addition, the primers were tested for amplification efficiency using qPCR. Each qPCR reaction contained 0.75 µL cDNA template, and a reaction master mix containing 6.25 µL SYBR Green Master Mix (Thermo Fisher Scientific) and 0.3 µM of each primer in a total reaction volume of 12.5 µL. The thermal profile used for qPCR was: 1 min at 95 °C; 40 cycles of 15 s at 95 °C and 1 min 60 °C. All qPCR assays were run as technical duplicates and each plate included a no template control. All assays were run on an Eco™ Real-Time PCR System (Illumina).
Quantification of sporozoite prevalence and abundance by quantitative PCR
To quantify sporozoite burden, DNA was first extracted from parasite-infected salivary glands macerated using sterile plastic pestles. Each tube was then spun down at 12,000 x g for 10 min to remove tissue debris and DNA was extracted following DNAzol’s manufactures’ instructions, with the addition of 6 µg of glycogen before ethanol precipitation (used as a DNA carrier).
For sporozoite quantification, the primers and hydrolysis probe targeting the P. falciparum single copy gene glutathione reductase (PfGR, PF3D7_1419800)51 were designed using Integrated DNA Technologies’ Real Time PCR tool (https://www.idtdna.com/scitools/Applications/RealTimePCR). Forward and reverse primer sequences were PfGR_F 5’-GCTGCCTCAGTTCATGATATTT-3’ and PfGR_R 5’-CTTATCCCTTCTCTCTACCAACAG-3’. The sequence for the fluorophore labelled probe was /56-FAM/TC CAG GCA T/ZEN/T ACG GAT TTG ACA CCA /3IABkFQ/. PfGR was amplified from NF54 genomic DNA and cloned into a pUC19 backbone to generate standard curves of known copy number. Each qPCR reaction contained 3 µL of template DNA, and a reaction master mix containing 5 µL PrimeTime Gene Expression Master Mix without ROX dye (QIAGEN, USA), 0.3 µM of each primer, and 0.1 µM of each probe. The thermal profile used for qPCR was: 1 min at 95 °C; 45 cycles of 15 s at 95 °C and 1 min 60 °C. All qPCR assays were run as technical duplicates and each plate included a no template control, a negative control (uninfected An. gambiae DNA) and a positive control (a linearized plasmid containing the PfGR amplicon sequence). All assays were run on an Eco™ Real-Time PCR System (Illumina).
RNAi gene silencing
Double strand RNA (dsRNA) was produced from a fragment of AgAkh1, AgAkh2, AgAkhR and GFP (as a negative control) with the universal T7 polymerase promoter sequence, 5’-TAATACGACTCACTATAGGG-3’, appended to the 5’ of each primer, and cloned into a pJET1.2 plasmid. Primer sequences are given for reference in Supplementary Table S1. The dsRNA was produced using a MEGAscript™ RNAi Kit and purified using a MEGAclear Transcription Clean-Up kit (Ambion). To confirm knockdown efficiency, 5- to 6-day old sugar-fed adult An. gambiae females were cold-anesthetized and injected with 69 nL of 5 µg/µL of dsGFP, dsAgAkh1, dsAgAkh2, dsAgAkhR, or dsAgAkh1 + dsAgAkhR using a Nanoject II injector (Drummond). Nine females from each treatment were sampled 1-, 3-, and 5-days post dsRNA injection, starved for 3 h (to induce AgAkh expression) and preserved in pools of 3 in TRIzol for further analysis. The silencing efficiencies of each dsRNA was determined using RT-qPCR and an AgAkh1 peptide ELISA. To determine the role of AKH signaling in P. falciparum sporogony, An. gambiae females were treated with dsAgAkh1, dsAgAkh1 + dsAgAkhR and dsGFP 3 dpib. A subset of mosquitoes was sampled 24 h post dsRNA injection to confirm knockdown using RT-qPCR and AgAkh1 ELISA analyses. The rest were sampled at 8 dpib for midgut oocyst enumeration and diameter measurements, and 14 dpib for salivary gland sporozoite enumeration. For oocyst diameter measurements, each midgut was imaged using a Keyence BZ-X700, ensuring oocysts in all focal planes were visible. ImageJ and Progress Capture Pro software were used to measure the diameter of the first 25 oocysts from the anterior end of each midgut. Diameters of all the oocysts in midguts with less than 25 oocysts were measured. Three biological replicates representing three independent infection assays were performed.
Peptide hormone injections
Synthetic AgAkh1 (pE-LTFTPAW-amide, purity >95%) and AgAkh2 (pE-VTFSRDWNA-amide, purity >95%) were purchased from New England PeptideTM. Dilutions of 1.69 pmol/µL (for a final dilution of 116.61 fmol/ mosquito) and 1.16 pmol/µL (for a final dilution of 80.04 fmol/ mosquito) of each peptide were prepared in 1X PBS from stocks of AgAkh1 and AgAkh2, respectively. Each female An. gambiae was intrathoracically injected with 69 nL of one of the two peptides using a Nanoject II injector (Drummond). Control groups were injected with 69 nL of PBS. To confirm the effect of the synthetic peptides on downstream genes in the AKH pathway (AgAkhR, AgLsdp1, and AgLp), 9 females from each treatment and control group were sampled 3 hours postinjection, preserved in pools of 3 in TRIzol and analyzed by RT-qPCR. To determine the effect of the synthetic AKH peptides on P. falciparum sporogony, infected An. gambiae were intrathoracically injected with synthetic AgAkh1 and AgAkh2 at 3 or 5 dpi. Controls included P. falciparum-infected mosquitoes injected with sterile PBS. Mosquitoes were dissected 8 dpib for oocyst enumeration and diameter measurements, and 14 dpib for salivary gland sporozoite quantification. Three biological replicates representing three independent infection assays were performed.
Harvesting P. falciparum-infected midgut supernatant
Anopheles gambiae were infected with P. falciparum at 1% gametocytemia and 50% hematocrit via SMFA as described above. Mosquitoes were then maintained on 10% sugar solution until day 3, 4, or 5 dpib when midguts were dissected in RPMI media (RPMI 1640 Medium, no glutamine, no phenol red, GibcoTM) and pooled as groups of three. Midgut pools were incubated for 24 h in 96-well cell culture plates (CELLTREAT Scientific) with either: (a) 200 µL of media comprising RPMI + 1.25% penicillin-streptomycin + 0.125% amphotericin, or (b) 200 µL of media comprising RPMI + 25% heat-inactivated lipid-rich FBS + 1.25% penicillin-streptomycin + 0.125% amphotericin. Controls included RPMI media only and supernatant from pooled midguts of age/time point-matched non-infectious blood-fed females incubated in both sets of media. Supernatant was carefully pipetted from each cell culture well into 1.5 mL low-bind Eppendorf tubes containing 10 µL protease inhibitor cocktail, snap-frozen in liquid nitrogen, and stored at −80 °C. The midguts from the P. falciparum-infected pools were analyzed for parasite burden using the PfGR DNA procedure as described above. Only pools that were positive for P. falciparum infection were used in the subsequent assays. Another subset was dissected at 8 dpi for oocyst enumeration by microscopy. To increase the supernatant concentration for injection, pools were further combined (n = 9 midgut supernatants total) and concentrated to a volume of 200 µL via vacuum centrifuge. Sixty-nine nanoliters of each concentrate was then injected into 5- to 6-day old sugar-fed female An. gambiae using a Nanoject II. Mosquitoes were sampled 1 h postinjection, pooled into groups of three and extracted for total RNA as aforementioned. An aliquot of the extracted RNA was subjected to RT-qPCR analysis to detect AgAkh1, AgAkh2, AgAkhR, AgLsdp1, and AgLp transcripts as per the conditions described above. Another subset of 9 supernatant-injected females were snap frozen in pools of 3 in liquid nitrogen for ELISA analysis. Three biological replicates representing supernatants from 3 independent infectious feeds injected into 3 different cohorts of sugar-fed mosquitoes was conducted.
Sample preparation for measuring AgAkh1 peptide levels
Peptide extraction
Mosquito samples stored from starvation, non-infectious blood feeding, P. falciparum blood feeding time points as well as RNAi assays, and midgut supernatant treatments were homogenized in 100 µL acidified methanol (90/9/1 MeOH: CH3OOH: H2O), each group included three biological replicates with a pool of 3 female mosquitoes. The homogenate was sonicated for 10 min and centrifuged at 16,110 x g at 4 °C for 20 min. The supernatant was transferred to a new 1.5 mL Eppendorf tube. The resultant tissue pellet was rinsed two more times with acidified methanol and combined with the collected supernatant. The combined supernatant was desiccated in a SpeedVac Concentrator and redissolved in 400 µL 1X PBS. Total peptide content was estimated using a Nanodrop 2000 spectrophotometer and used to normalize across the samples.
Generation and purification of Anti-AgAkh1 antibodies
AgAkh1 peptide ({pGlu}LTFTPAW) conjugated to KLH via the C-terminus was used to immunize two rabbits to generate polyclonal antibodies. The antibodies were generated by GenScript (Piscataway, NJ, USA), following their standard polyclonal antibody production protocol. Due to low antigenicity of this epitope, a total of five boosts were carried out. Anti-AgAkh1 specific antibodies were purified from the rabbit anti-sera using {pGlu}LTFTPAWCOOH peptide (New England Peptide, Gardner, MA, USA) conjugated to Dynabeads™ M-270 Epoxy (Thermo Fisher Scientific) following manufacturer’s instructions. The IgG titers of the purified Anti-AgAkh1 antibodies was determined using Easy-Titer™ Rabbit IgG assay kit (Thermo Fisher Scientific).
AgAkh1 competitive peptide Enzyme-Linked Immunosorbent Assay
Maxisorp 96-well ELISA plates (Nunc, Fisher Scientific) were incubated and covered overnight at 4 °C with 100 µL AgAkh1 peptide at 1 μg/mL in 1X PBS. Plates were washed three times with PBS-Tween 20 (0.1%) (PBST20) and blocked with 1% bovine serum albumin (BSA)/1X PBS for 1 h at room temperature. For each ELISA, standard curves were generated using ten-fold dilutions of the AgAkh1 peptide (0, 0.001, 0.01, 0.1, 1, 10, 100 µg/mL). The standards and the mosquito samples (described above) were spiked with Anti-AgAkh1 Abs at 5 µg/mL and incubated for 10 min at room temperature, and 100 µL of each standard/sample was then added into wells in triplicates, covered, and incubated at room temperature for 1 h. Plates were then washed three times with PBST20 and incubated with 100 μL of a horseradish peroxidase (HRP)-conjugated anti-Rabbit IgG (Thermo Scientific) diluted to 1:5,000 in 0.5% BSA/ 1X PBS, covered and incubated for 1 h at room temperature. Plates were then washed again three times with PBST20 and 100 μL of TMB (3,3′,5,5′-tetramethylbenzidine) microwell peroxidase substrate (Kirkegaard & Perry Laboratories) was added to each well. The reaction was stopped after five min by adding 100 μL of 1 M phosphoric acid. The optical density was read at 450 nm and 570 nm using SynergyMx Multi-Mode Microplate Reader. Background was corrected for by subtracting the OD value at 450 nm from the OD value at 570 nm. A standard curve was generated and used to estimate the amount of AgAkh1 peptide under each different treatment condition.
Statistics and reproducibility
The statistical methods used to analyze each experiment are detailed in the figure legends. The cycles to threshold (Ct) values of each sample were used to generate relative gene expression levels and normalized to AgRPL32 which does not change with starvation, blood feeding and infection. Fold change in expression levels between different treatments was determined by double delta Ct (−∆∆Ct). Resultant transcriptomic expression data was subjected to a Shapiro test for normality and Levine’s test of homogeneity, and transformations to generate normality before non-parametric tests were attempted. The lme4 (v 1.1)52, and multcomp (v 1.4)53 packages were used to run linear mixed-effects models analyzing the differences between sporozoite genome copy number and oocyst diameter data, using treatment (PBS, SAgAkh1, SAgAkh2, dsGFP, dsAgAkh1) as a fixed effect and biological replicate as a random effect. These packages were also used to analyze oocyst intensities between treatments and their respective controls using a zero-inflated generalized linear model at a 95% confidence interval. Differences in peptide titers were detected by a Kruskal-Wallis test and two-sample Wilcoxon test. Midgut oocyst and salivary gland sporozoite data were analyzed using Chi-square test. Effect size of dsRNA and synthetic peptide treatments on oocyst diameter and sporozoite genome copy number was estimated using Cohen’s d effect size. Similarly, the effect size of midgut supernatant treatment on peptide levels were estimated using Cohen’s d effect size. Statistical analyses were completed in GraphPad Prism 7 and R version 3.6.354. All statistical tests were analyzed at an α = 0.05.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
The source data for all graphs and charts are provided in Supplementary Data 1 and Figshare (https://doi.org/10.6084/m9.figshare.21771071.v1).
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Acknowledgements
The authors thank Professors P. Eggleston and H. Hurd for the An. gambiae (Keele) strain and Dr. Megan Miller for editorial assistance. This study was funded in part by the University of Florida Preeminence Initiative through the College of Veterinary Medicine and the Emerging Pathogens Institute (R.R.D). All images were created in Biorender.com.
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V.O.N., T.H. and R.R.D. designed research; V.O.N., A.L-V, and B.L-G performed research and generated data; H.C. and T.H. contributed on the analyses/tools; V.O.N. and H.C. analyzed data; and V.O.N, T.H., H.C. and R.R.D. wrote the paper. All authors contributed to the drafting of the manuscript.
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The human blood and serum used for mosquito bloodmeals, and parasite cultures was obtained commercially (LifeSouth Community Blood Center).
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Nyasembe, V.O., Hamerly, T., López-Gutiérrez, B. et al. Adipokinetic hormone signaling in the malaria vector Anopheles gambiae facilitates Plasmodium falciparum sporogony. Commun Biol 6, 171 (2023). https://doi.org/10.1038/s42003-023-04518-6
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DOI: https://doi.org/10.1038/s42003-023-04518-6
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