Abstract
Nexilin (NEXN) plays a crucial role in stabilizing the sarcomeric Z-disk of striated muscle fibers and, when mutated, leads to dilated cardiomyopathy in humans. Due to its early neonatal lethality in mice, the detailed impact of the constitutive homozygous NEXN knockout on heart and skeletal muscle morphology and function is insufficiently investigated. Here, we characterized a constitutive homozygous CRISPR/Cas9-mediated nexn knockout zebrafish model. We found that Nexn deficient embryos developed significantly reduced cardiac contractility and under stressed conditions also impaired skeletal muscle organization whereas skeletal muscle function seemed not to be affected. Remarkably, in contrast to nexn morphants, CRISPR/Cas9 nexn−/− knockout embryos showed a milder phenotype without the development of a pronounced pericardial edema or blood congestion. nexn-specific expression analysis as well as whole transcriptome profiling suggest some degree of compensatory mechanisms. Transcripts of numerous essential sarcomeric proteins were massively induced and may mediate a sarcomere stabilizing function in nexn−/− knockout embryos. Our findings demonstrate the successful generation and characterization of a constitutive homozygous nexn knockout line enabling the detailed investigation of the role of nexn on heart and skeletal muscle development and function as well as to assess putative compensatory mechanisms induced by the loss of Nexn.
Similar content being viewed by others
Introduction
According to the World Health Organization (WHO), cardiovascular disease is the leading cause of death worldwide, accounting for approximately 17.9 million deaths per year1. One of the main causes of heart failure is dilated cardiomyopathy (DCM), which leads to the dilation of the heart muscle and thus to impaired contractile function that can cause heart failure and sudden cardiac death2,3. DCMs are frequently caused by mutations in genes encoding for proteins such as Titin, α-Actin, Myosin Heavy Chain or Nexilin (NEXN) that make up the sarcomere, the basic contractile unit of striated muscle2,4,5.
NEXN was first identified in a screen for cardiac proteins associated with DCM in humans6. As a F-actin-binding protein, NEXN appears to play a role in linking the actin cytoskeleton to the plasma membrane and is predominantly expressed in cardiomyocytes and skeletal muscle cells7. We have shown that Morpholino (MO)-induced knockdown of nexn disrupts sarcomeric integrity and Z-disc architecture leading to DCM in zebrafish. Similar structural Z-disk alterations were observed in patients carrying NEXN mutations6. Furthermore, NEXN deficiency in mice was shown to be associated with the development of DCM8,9, endomyocardial fibroelastosis, characterized by increased deposition of collagen and elastin, as well as the intrauterine or early postnatal lethality6,10. Recent studies suggest that NEXN is also involved in the initiation and formation of T-tubules, which are required for Ca2+ signaling from the sarcoplasmic reticulum in cardiomyocytes11. Interestingly, although NEXN is also highly expressed in skeletal muscle, loss of NEXN appears to have more deleterious effects on cardiac muscle6. Patients with NEXN mutations suffer primarily from cardiac dysfunction and do not show skeletal muscle impairments. In zebrafish, defective skeletal muscle structure and function were also found only after a drastic increase in workload6.
In the study presented here, we established and characterized a CRISPR/Cas9-mediated homozygous zebrafish model of constitutive Nexn deficiency. The ability of zebrafish to survive up to 5 days post fertilization without a functioning cardiovascular system allows the detailed analysis of the impact of loss of the important sarcomeric protein Nexn. We found that Nexn-deficient embryos develop a progressive form of DCM even under basal conditions and additional skeletal muscle defects under stress conditions. Interestingly, the muscular defects observed here are milder than those seen after MO-mediated nexn knockdown. Analysis of transcriptional profiles suggests that there are certain compensatory transcriptional changes in CRISPR/Cas9-mediated Nexn-deficient zebrafish embryos, namely the induction of expression of essential sarcomeric proteins, which may mitigate the detrimental loss of Nexn. This zebrafish line will allow to study the role of nexn in cardiac and skeletal muscle development and function in more detail and the possible compensatory mechanisms triggered by mutation of nexn.
Results
CRISPR/Cas9-induced homozygous knockout of nexn causes progressive cardiac dysfunction without affecting skeletal muscle function in zebrafish
Several studies in different animal models and cardiomyopathy patients have shown that loss of NEXN is leading to DCM which is characterized by an impaired contractility of the heart6,10,12. Homozygous loss of NEXN mostly causes intrauterine or early postnatal lethality12,13, impeding the assessment of effects on early muscle development as well as cardiac and skeletal muscle function. In addition to previous studies demonstrating the effects of MO-mediated knockdown of nexn in zebrafish, we here generated a constitutive homozygous CRISPR/Cas9-induced nexn knockout zebrafish line. A 32 nucleotide deletion within exon 2 leads to the establishment of a premature stop codon causing the termination of protein translation at position 49 within the first actin-binding-domain of Nexn (Fig. 1a). Nexn loss was confirmed via immunoblot analysis where we found a significant reduction of Nexn protein levels in homozygous nexn−/− embryos (N = 4, p = 0.0286) (Fig. 1b,c and Supplementary Fig. S1). Nevertheless, quantitative RT-PCR (qPCR) analysis revealed that nexn mRNA levels were unaltered in nexn−/− embryos (N = 4, p = 0.1429) (Fig. 1d). Additionally, RNA in situ hybridization showed similar nexn expression patterns and quantity in skeletal muscle and heart in both, nexn+/+ and nexn−/− embryos at 24 h post fertilization (hpf) (Fig. 1e). Furthermore, the Kaplan–Meier curve did not show increased mortality of nexn−/− compared to nexn+/+ embryos between 24 and 120 hpf (Fig. 1f). In fact, nexn−/− fish survive to adulthood, are fertile and could be kept for several years. Nevertheless, adult fish showed a tendency towards increased heart weight/body weight ratio in nexn−/− compared to nexn+/+ fish (N = 3, p = 0.1000) (Supplementary Fig. S2), suggesting a putative late-onset of cardiomyopathy.
Generation of zebrafish nexn knockout by CRISPR/Cas9 gene editing. (a) Structure of the nexn gene and protein. Nexn consists of a central coiled coil domain (CC) flanked by two actin-binding domains (ABD) and a C-terminal immunoglobulin superfamily class (IGcam). The deletion of 32 nucleotides in exon 2 by CRISPR/Cas9 editing leads to a frameshift and premature stop codon, and thereby to a termination of the Nexn translation after 49 amino acids within the first actin-binding domain. (b,c) Immunoblot and quantification of protein lysates of nexn−/− embryos showing reduced Nexn protein levels compared to nexn+/+ embryos at 72 hpf (N = 4, mean ± SD, p < 0.0286 using Wilcoxon test); original blot shown in Supplementary Fig. S1. (d) Quantitative real-time PCR of nexn−/− embryos showing similar nexn mRNA levels compared to nexn+/+ embryos at 72 hpf (N = 4, mean ± SD, p = 0.149 using Wilcoxon test). (e) in situ hybridization shows nexn expression in heart and skeletal muscle at a similar level in nexn−/− and nexn+/+ embryos at 24 hpf (N = 3, n = 15). (f) nexn+/+ and nexn−/− embryos do not show differences regarding survival rate at any developmental stage (N = 3 with a total number of 87 and 62 embryos, respectively, mean). ns not significant, *p < 0.05.
Interestingly, in contrast to the MO-mediated knockdown of nexn which led to the development of a pronounced pericardial edema, reduced ventricular fractional shortening (FS) and impaired skeletal muscle organization (Supplementary Fig. S3), overall morphology of nexn+/+ and nexn−/− embryos did not differ significantly (Fig. 2a,b and Supplementary Fig. S4). To assess skeletal muscle function, birefringence analysis was performed. Signal intensity was at the same level in nexn−/− and nexn+/+ embryos at 48 and 72 hpf (48 hpf: N = 3, n = 15, p = 0.1301); (72 hpf: N = 3, n = 14/15, p = 0.1201) but significantly reduced in nexn−/− embryos at 120 hpf (N = 3, n = 15, p < 0.0001) (Fig. 2a,c). Nevertheless, muscle structure of nexn−/− embryos, assessed by the immunostaining of the sarcomeric protein Titin, was indistinguishable from nexn+/+ embryos at 48, 72 and 120 hpf (N = 3, n = 15) (Fig. 2d). Also responsiveness to mechanical stimuli seemed not to be affected at any stage (N = 3, p > 0.9999 for all) (Fig. 3a). For detailed analysis of the touch-evoked flight response, we calculated the velocity and acceleration of nexn+/+ and nexn−/− embryos. Movement is illustrated representative for 48, 72 and 120 hpf (Supplementary Fig. S5). Velocity appeared increased in nexn−/− compared to nexn+/+ embryos at 48 hpf (N = 3, n = 15, p = 0.0017) but not at later time points (72 hpf: N = 3, n = 14/15, p = 0.8709); (120 hpf N = 3, n = 15, p = 0.3610). Acceleration was unaffected in nexn−/− compared to nexn+/+ embryos at all analyzed stages (48 hpf: N = 3, n = 15, p = 0.2121); (72 hpf: N = 3, n = 14/13, p = 0.8709); (120 hpf N = 3, n = 15, p = 0.3610) (Fig. 3b,c). To analyze heart function in nexn−/− embryos, heart rate, enddiastolic ventricular diameter (EVD) and FS were assessed at 48, 72 and 120 hpf. Heart rate was significantly increased in nexn−/− embryos at 48 hpf (N = 3, n = 15, p = 0.0404) but did not differ at later developmental stages (72 hpf: N = 3, n = 15, p = 0.6927); (120 hpf: N = 3, n = 15, p = 0.2300) (Fig. 3d). EVD was significantly reduced in 48 hpf nexn−/− embryos (N = 3, n = 15, p = 0.0350) but at similar levels as in nexn+/+ embryos at 72 and 120 hpf (72 hpf: N = 3, n = 15, p = 0.6099); (120 hpf: N = 3, n = 15, p = 8075) (Fig. 3e). FS was not significantly affected at 48 hpf (N = 3, n = 15, p = 0.2315) but significantly decreased at 72 as well as 120 hpf in nexn−/− compared to nexn+/+ embryos (72 hpf: N = 3, n = 15, p = 0.0300); (120 hpf: N = 3, n = 14/15, p = 0.0141) (Fig. 3f). Immunostaining against Meromyosin (MF20, red) and atrial Myosin Heavy Chain (S46, green) showed proper cardiac differentiation and specification into ventricle and atrium in nexn−/− embryos (N = 3, n = 15) (Fig. 3g). Taken together, these findings are in line with already published results of Nexn loss leading to cardiac dysfunction without significantly interfering with skeletal muscle structure and function6.
nexn knockout does not lead to severe skeletal muscle deficits. (a) Brightfield and birefringence images do not reveal phenotypical differences between nexn+/+ and nexn−/− embryos at 72 hpf. (b) Percentage of embryos showing phenotypical abnormalities does not differ between nexn+/+ and nexn−/− embryos at 48, 72 or 120 hpf (N = 3, mean ± SD, 48 hpf: p > 0.9999, 72 hpf: p = 0.6000, 120 hpf: p > 0.9999 using Mann–Whitney test). (c) Densitometric quantification of birefringence signals of nexn−/− and nexn+/+ embryos reveals significant differences at 120 (N = 3, n = 15, mean ± SD, p < 0.0001 using two-tailed t-test) but not 48 or 72 hpf (N = 3, n = 14/15, mean ± SD, 48 hpf: p = 0.1301, 72 hpf: p = 0.1201 using two-tailed t-test). (d) Immunostaining of nexn+/+ and nexn−/− embryos at 48, 72 and 120 hpf against Titin does not show muscle disruption. Scale bar (1 µm) refers to all images. ns not significant, ****p < 0.0001. Exact values (mean ± SD) are shown in Supplementary Table 1.
nexn knockout causes reduced cardiac function in zebrafish. (a) nexn+/+ and nexn−/− embryos do not show differences regarding responsiveness to mechanical stimuli at any developmental stage (N = 3, mean ± SD, p > 0.9999 for all using Mann–Whitney test). (b) nexn−/− embryos showing increased velocity at 48 hpf (N = 3, n = 15, mean ± SD, p = 0.0017 using two-tailed t-test) but not at later time points (N = 3, n = 14/15, mean ± SD, 72 hpf: p = 0.8709, 120 hpf: p = 0.3610 using two-tailed t-test). (c) Acceleration is at a similar level in nexn+/+ and nexn−/− embryos at 48, 72 and 120 hpf (N = 3, n = 14/15, mean ± SD, 48 hpf: p = 0.2121, 72 hpf: p = 0.9312, 120 hpf: p = 0.7324 using two-tailed t-test). (d) Heart rate was increased in nexn−/− embryos at 48 hpf (N = 3, n = 15, mean ± SD, p = 0.0404 using two-tailed t-test) but at a similar level as in nexn+/+ embryos at 72 and 120 hpf (N = 3, n = 15, mean ± SD, 72 hpf: p = 0.6927, p = 0.2300 using two-tailed t-test). (e) Whereas enddiastolic ventricular diameter was significantly decreased in nexn−/− embryos at 48 hpf (N = 3, n = 15, mean ± SD, p = 0.0350 using two-tailed t-test), it was not affected at 72 or 120 hpf (N = 3, n = 15, mean ± SD, 72 hpf: p = 0.6099, 120 hpf: p = 0.8075 using two-tailed t-test). (f) Analysis of ventricular fractional shortening does not show altered heart contractility in nexn−/− at 48 hpf (N = 3, n = 15, mean ± SD, p = 0.2315 using two-tailed t-test) but reduced contractility at 72 and 120 hpf (N = 3, n = 15, mean ± SD, 72 hpf: p = 0.0300, 120 hpf: p = 0.0141 using two-tailed t-test). (g) MF20/S46 staining shows properly differentiated ventricle and atrium in nexn−/− and nexn+/+ embryos at 72 hpf. Scale bar (20 µM) refers to both images. FS fractional shortening, ns not significant, *p < 0.05, **p < 0.01. Exact values (mean ± SD) are shown in Supplementary Table 1.
Increased workload results in impaired skeletal muscle function in Nexn deficient zebrafish embryos
In nexn morphant embryos, it was shown that skeletal muscle dysfunction can be provoked by increasing their workload via electrical stimulation6. Here, we aimed to mimic this approach by increasing the viscosity of the medium by adding 1% methylcellulose and incubating embryos for 2 h14,15. Stressing the embryos did not lead to morphological alterations of nexn−/− embryos compared to nexn+/+ at 48, 72 or 120 hpf (Fig. 4a,b and Supplementary Fig. S4). Similar to the unstressed embryos, skeletal muscle organization and function was tested. Birefringence analysis did not reveal altered signal intensity in nexn−/− embryos at 48 and 72 hpf (48 hpf: N = 3; n = 14/15, p = 0.6516); (72 hpf: N = 3, n = 15, p = 0.2193). Again, 120 hpf nexn−/−embryos showed a significant reduction in birefringence signal, also under stress conditions (N = 3, n = 15, p = 0.0046) (Fig. 4c). Immunostaining of Titin showed organized myofibrils at 48 and 72 hpf nexn−/− embryos after methylcellulose stress. Surprisingly, 120 hpf nexn−/− embryos seemed to have unaltered myofibrillar assembling in caudal areas (data now shown) of their skeletal muscles, whereas they showed unorganized myofibrils in more cranial regions (exact location shown in schematic illustration) (Fig. 4d). Touch-evoked flight response was unaltered in nexn−/− compared to nexn+/+ embryos independently of their developmental stage (48 hpf: N = 3, p > 0.9999 for all) (Fig. 5a). A more detailed look at the motility of the embryos under stress conditions revealed no significant changes regarding velocity (48 hpf: N = 3, n = 15, p = 0.7875); (72 hpf: N = 3, n = 13, p = 0.5478); (120 hpf: N = 3, n = 15, p = 0.5202) or acceleration (48 hpf: N = 3, n = 15, p = 0.2460); (72 hpf: N = 3, n = 13, p = 0.1754); (120 hpf: N = 3, n = 15, p = 0.9284) in 48, 72 or 120 hpf nexn−/− embryos. (Fig. 5b,c). Movement of nexn+/+ and nexn−/− embryos is illustrated representatively (Supplementary Fig. S5). Moreover, cardiac function under stress conditions was assessed. Heart rate of nexn−/− embryos was at a similar level when compared to nexn+/+ embryos independently of their developmental stage (48 hpf: N = 3, n = 15, p = 0.1738); (72 hpf: N = 3, n = 15, p = 0.3121); (120 hpf: N = 3, n = 15, p = 0.8520) (Fig. 5d). Whereas EVD was not affected at 48 and 72 hpf (48 hpf: N = 3, n = 15, p = 0.1807); (72 hpf: N = 3, n = 15, p = 0.4152), 120 hpf nexn−/− embryos had a significantly increased EVD (N = 3, n = 14/15, p = 0.0117) (Fig. 5e). Similar to unstressed conditions, stressed nexn−/− embryos developed a significant reduction of FS starting from 72 hpf (72 hpf: N = 3, n = 15, p = 0.0379); (120 hpf: N = 3, n = 14/15, p = 0.0020) whereas FS was unaltered at 48 hpf (N = 3, n = 15, p = 0.1807) (Fig. 5f). These effects are in line with previous studies showing myofibrillar disruption in skeletal muscle tissue under stress conditions6.
Increased muscular workload causes skeletal muscle disruption in later developmental stages of nexn knockout embryos. (a) Brightfield and birefringence images do not reveal phenotypical differences between nexn+/+ and nexn−/− embryos at 72 hpf. (b) Percentage of embryos showing phenotypical abnormalities does not differ between nexn+/+ and nexn−/− embryos at 48, 72 or 120 hpf (N = 3, mean ± SD, 48 hpf: p > 0.9999, 72 hpf: p = 0.8000, 120 hpf: p > 0.9999 using Mann–Whitney test). (c) Densitometric quantification of birefringence signals of nexn−/− and nexn+/+ embryos after stress reveals significant differences at 120 (N = 3, n = 15, mean ± SD, p < 0.0046 using two-tailed t-test) but not 48 or 72 hpf (N = 3, n = 14/15, mean ± SD, 48 hpf: p = 0.6516, 72 hpf: p = 0.2193 using two-tailed t-test). (d) Immunostaining of nexn+/+ and nexn−/− embryos after stress against Titin does not show altered muscle fibers at 48 and 72 hpf but severe muscle disruption in cranial regions (location shown in schematic illustration; created with biorender.com) at 120 hpf. Scale bar (1 µm) refers to all images. ns not significant, **p < 0.01. Exact values (mean ± SD) are shown in Supplementary Table 1.
Increased muscular workload does not lead to aggravated cardiac dysfunction in nexn knockout zebrafish. (a) nexn+/+ and nexn−/− embryos do not show significant differences regarding responsiveness to mechanical stimuli at 48, 72 or 120 hpf (N = 3, mean ± SD, p > 0.9999 for all using Mann–Whitney test). (b) Maximal velocity was not affected at any developmental stage after increasing workload (N = 3, n = 15, mean ± SD, 48 hpf: p = 0.7875, 72 hpf: p = 0.5428, 120 hpf: p = 0.5202 using two-tailed t-test). (c) Maximal acceleration is at a similar level in nexn+/+ and nexn−/− embryos at 48, 72 and 120 hpf (N = 3, n = 15, mean ± SD, 48 hpf: p = 0.2460, 72 hpf: p = 0.1754, 120 hpf: p = 0.9284 using two-tailed t-test). (d) Heart rate of nexn−/− embryos was at a similar level as in nexn+/+ embryos at 48, 72 and 120 hpf (N = 3, n = 15, mean ± SD, 48 hpf: p = 0.1738, 72 hpf: p = 0.3121, 120 hpf: p = 0.8520 using two-tailed t-test). (e) Increased workload does not affect enddiastolic ventricular diameter at 48 and 72 hpf (N = 3, n = 15, mean ± SD, 48 hpf: p = 0.1807, 72 hpf: p = 0.4152 using two-tailed t-test) but leads to increased enddiastolic diameter at 120 hpf (N = 3, n = 15, mean ± SD, p = 0.0017 using two-tailed t-test). (f) Analysis of ventricular fractional shortening does not show altered heart contractility in nexn−/− compared to nexn+/+ embryos at 48 hpf (N = 3, n = 15, mean ± SD, p = 0.1538 using two-tailed t-test) but reduced fractional shortening at 72 and 120 hpf (N = 3, n = 15, mean ± SD, 72 hpf: p = 0.0379, 120 hpf: p = 0.0020 using two-tailed t-test). FS fractional shortening, ns not significant, *p < 0.05, **p < 0.01. Exact values (mean ± SD) are shown in Supplementary Table 1.
Isoproterenol-induced heart rate increase does not cause aggravating symptoms in Nexn deficient zebrafish embryos
As we observed an increased heart rate in unstressed nexn−/− compared to unstressed nexn+/+ embryos at 48 hpf but not at later time points or under stressed conditions, we were interested if some of the observed effects may be dependent on elevated heart rate (and thus higher workload) at certain stages of development. For increasing heart rate, zebrafish were treated with Isoproterenol16 (N = 3, n = 15 for all; (48 hpf nexn+/+: p < 0.0001); (48 hpf nexn−/−: p = 0.0085); (72 hpf nexn+/+: p < 0.0001); (72 hpf nexn−/−: p < 0.0001); (120 hpf nexn+/+: p = 0.0002); (120 hpf nexn−/−: p = 0.0001) (Fig. 6a). Isoproterenol-induced increase of heart rate did not lead to morphological changes at any developmental stage (Fig. 6b,c and Supplementary Fig. S4). A difference in heart rate between nexn+/+ and nexn−/− embryos could not be observed at 48 and 72 hpf (48 hpf: N = 3, n = 15, p = 0.3112); (72 hpf: N = 3, n = 15, p = 0.3690) but heart rate was slightly increased in nexn−/− embryos at 120 hpf (N = 3, n = 14/15, p = 0.0350) (Fig. 6d). EVD was not affected at any developmental stage (48 hpf: N = 3, n = 15, p > 0.9999); (72 hpf: N = 3, n = 15, p = 0.7580); (120 hpf: N = 3, n = 14/15, p = 0.0701) (Fig. 6e). Similar to unstressed nexn−/− embryos and nexn−/− embryos being stressed with methylcellulose, also nexn−/− embryos being treated with Isoproterenol did not show reduced FS compared to nexn+/+ embryos at 48 hpf (N = 3, n = 15, p = 0.3284). FS was significantly reduced starting from 72 hpf (72 hpf: N = 3, n = 15, p = 0.0099); (120 hpf: N = 3, n = 14/15, p = 0.0498) (Fig. 6f). Taken together, increasing heart rate by Isoproterenol did augment cardiac contractile dysfunction in Nexn deficient embryos.
Increasing heart rate does not cause severe changes in cardiac functionality. (a) Isoproterenol treatment increases heart rate in nexn+/+ and nexn−/− embryos at all developmental stages (N = 3, n = 14/15, mean ± SD, 48 hpf nexn+/+: p < 0.0001, 48 hpf nexn−/−: p = 0.0085, 72 hpf nexn+/+: p < 0.0001, 72 hpf nexn−/−: p < 0.0001, 120 hpf nexn+/+: p = 0.0002, 120 hpf nexn−/−: p = 0.0001 using two-tailed t-test). (b) Brightfield images do not reveal phenotypical differences between nexn+/+ and nexn−/− embryos at 72 hpf. (c) Percentage of embryos showing phenotypical abnormalities does not differ between nexn+/+ and nexn−/− embryos at 48, 72 or 120 hpf (N = 3, mean ± SD, p > 0.9999 for all using Mann–Whitney test). (d) Heart rate of nexn−/− embryos is at a similar level as in nexn+/+ embryos at 48, and 72 hpf but increased at 120 hpf (N = 3, n = 15, mean ± SD, 48 hpf: p = 0.3112, 72 hpf: p = 0.3690, 120 hpf: p = 0.0350 using two-tailed t-test). (e) Increased heart rate does not affect enddiastolic ventricular diameter at 48, 72 and 120 hpf (N = 3, n = 15, mean ± SD, 48 hpf: p > 0.9999, 72 hpf: p = 0.7588, 120 hpf: p = 0.0701 using two-tailed t-test). (f) Analysis of ventricular fractional shortening does not show altered heart contractility in nexn−/− compared to nexn+/+ embryos at 48 hpf (N = 3, n = 15, mean ± SD, p = 0.3284 using two-tailed t-test) but reduced fractional shortening at 72 and 120 hpf (N = 3, n = 15, mean ± SD, 72 hpf: p = 0.0099, 120 hpf: p = 0.0498 using two-tailed t-test). FS fractional shortening, ns not significant, *p < 0.05, **p < 0.01. Exact values (mean ± SD) are shown in Supplementary Table 1.
The fact that the cardiac phenotype in nexn−/− embryos was not as severe as the phenotype observed in nexn morphants, indicates that CRISPR/Cas9-induced nexn knockout might trigger compensatory mechanisms. As shown by qPCR and nexn-specific in situ hybridization, we found that nexn mRNA was not downregulated in nexn−/− embryos (Fig. 1), implying that the nonsense-mediated mRNA decay (NMD) pathway was not induced by the 32 nucleotide deletion within the nexn gene and thereby classical genetic compensation mechanisms, as for example observed in CRISPR-mediated Bag3 mutants15, might not be triggered in nexn knockout embryos. Nevertheless, to assess whether compensatory transcriptional mechanisms were present in nexn−/− embryos, we conducted RNA-sequencing analysis. The PCA showed nexn+/+ and nexn−/− clusters that were clearly separated by the first principal component indicating that variances in the data set were most likely caused by the mutation (Fig. 7a). Using differential expression analysis, we found 2094 genes being significantly up- (log2(FC) > 0.5) and 968 genes being significantly downregulated (log2(FC) < -0.5), (adjusted p < 0.05) in nexn−/− embryos (Supplementary Table 2). Concordant to our qRT-PCR results (Fig. 1d), nexn mRNA levels were also unaltered according to the RNA-sequencing data set (adjusted p = 1.14E−05, log2(FC) = −0.896133743). To identify significantly enriched GO-terms, we performed Gene Set Enrichment Analysis using the biological processed terms of the GO database and found muscle structure development to be activated in nexn−/− embryos (Supplementary Fig. S5) and showing an overrepresentation of upregulated genes in nexn−/− embryos (normalized ES = 1.84, adjusted p = 1.14E−07) (Fig. 7b). Having a closer look at this GO-term, we found several genes encoding for sarcomeric proteins being significantly upregulated in nexn−/− embryos including different members of the Myosin, Tropomyosin and Troponin family which are highlighted in the volcano plot and heat map (Fig. 7c,d). In addition to RNASeq, qPCR analysis confirmed the significant upregulation of myhb, tnni2b.1, cmlc1, tnnt2c, tnnt2a and tpm4b transcript levels in nexn−/− embryos (N = 4, p = 0.0286 for all) (Fig. 7e). In contrast, transcript levels of those genes were unaltered in nexn MO-injected embryos (N = 3; p = 0.7000 for all) (Fig. 7f).
Nexn deficiency leads to upregulation of several genes encoding for sarcomeric proteins. (a) Principal component analysis (PCA) plot showing nexn+/+ and nexn−/− embryos (72 hpf) being clearly separated by PC1 (N = 2, n = 25). (b) Gene set enrichment analysis of the Gene Ontology term “muscle structure development” showing an overrepresentation of upregulated genes in nexn−/− embryos (normalized ES = 1.84; adjusted p = 1.14E−07). (c) Volcano plot of differentially expressed genes in nexn−/− compared to nexn+/+ embryos. Upregulated genes shown in red and downregulated genes shown in blue (adjusted p-value < 0.05 and |log2(FC)| > 0.5). Selected genes are labeled. (d) Heatmap showing selected genes of interest with clear difference regarding expression between nexn+/+ and nexn−/− embryos. High read counts shown in red and low read counts in blue. (e) Quantitative real-time PCR showing significantly increased myhb, tnni2b.1, cmlc1, tnnt2c, tnnt2c, tnnt2a and tpm4b transcript levels in nexn−/− compared to nexn+/+ embryos at 72 hpf. (f) Quantitative real-time PCR showing unaltered levels of myhb, tnni2b.1, cmlc1, tnnt2c, tnnt2c, tnnt2a and tpm4b in nexn MO-injected embryos compared to Ctrl MO-injected embryos at 72 hpf. ns not significant, *p < 0.05. Exact values (mean ± SD) are shown in Supplementary Table 1.
These findings suggest that the compensatory transcriptional induction of the expression of essential sarcomeric proteins might mitigate the detrimental loss of Nexn in nexn−/− embryos.
Discussion
In humans, mutations in the NEXN gene were described to cause DCM which is characterized by a dilation of the myocardium and therefore an impaired cardiac contractility6. Similar pathological cardiac alterations were observed after Nexn knockout in mice and the MO-mediated knockdown of nexn in zebrafish6,8,9. Nevertheless, although the critical function of NEXN in the heart appears to be conserved across vertebrate species, its detailed structural or molecular role is still insufficiently understood. Here, we established and characterized for the first time a CRISPR/Cas9-induced homozygous zebrafish model of constitutive Nexn deficiency to assess the detailed role of Nexn loss in the development and function of the heart but also skeletal muscle. We found Nexn deficient embryos to develop a progressive form of DCM without involvement of skeletal muscles under physiological conditions. After increasing workload, also skeletal muscle developed a myopathic phenotype. Remarkably, the observed pathologies in the CRISPR/Cas9 mutants were milder than the defects evoked by the MO-mediated knockdown of nexn, suggesting compensatory mechanisms to be induced in nexn−/− mutants. nexn mRNA was not found degraded by the NMD pathway in the CRISPR/Cas9 mutants, likely excluding classical genetic compensation as compensatory route. Nevertheless, by transcriptional profiling, the massive transcriptional activation of important sarcomeric proteins was found, implying their upregulation to contribute to the protection of the sarcomere from Nexn deficiency induced Z-disk destabilization.
In line with already published data of MO-mediated Nexn deficiency in zebrafish, CRISPR/Cas9-induced nexn−/− knockout embryos showed a reduced cardiac contractility6. This effect appeared starting from 72 hpf, suggesting a progressive impairment of the heart muscle. Indeed, it’s also known from patients with NEXN mutations that their medical conditions are deteriorating over time17. Interestingly, in contrast to one of the main symptoms of DCM, we did not observe an enlarged and dilated ventricle in nexn−/− embryos under physiological conditions. In detail, at 48 hpf the EVD of Nexn deficient embryos was even smaller compared to nexn+/+ embryos which was accompanied by an increased heart rate. The abolishment of this phenotype at later developmental stages could indicate a progressive dilation of the ventricle. This hypothesis is supported by the finding that nexn−/− embryos developed an enlarged ventricle at 120 hpf when increasing the workload on the heart. Additionally, this could also be caused by different functions of nexn during different stages of development which may provide a basis for further impairments at later stages. Whereas the most studied function of nexn is the stabilization of the sarcomeric Z-disk6, it was also shown that nexn seems to be involved in the formation of cardiac T-tubules. Thus, nexn obviously plays a role in Ca2+-signaling as T-tubules are critical for the coordinated Ca2+-flux from the sarcoplasmic reticulum into cardiomyocytes11. Recent studies suggest high serum Ca2+ levels being associated with a reduced EVD18. In this context, the further investigation of the role of nexn in Ca2+-signaling would be interesting. Furthermore, it remained unclear if reduced EVD is resulting in an increased heart rate in nexn−/− embryos or vice versa. It is known that a smaller ventricle is leading to an elevated heart rate to compensate for the reduced ejection fraction19. In contrast, also increased heart rates appear to be able to lead to the development of smaller ventricles20,21. Interestingly, heart rate reduction in children suffering from DCM seems to be able to improve cardiac contractility and ejection fraction22. In this context, we induced tachycardia in nexn+/+ and nexn−/− embryos by incubating them with Isoproterenol at 48, 72 and 120 hpf. In contrast to literature, an increase of heart rate could already be seen at 48 hpf16. At 72 and 120 hpf, Isoproterenol-mediated induction of tachycardia did not lead to any additional negative effects on contractile performance compared to unstressed embryos. Strikingly, after isoproterenol treatment, EVDs of nexn+/+ and nexn−/− embryos were indistinguishable suggesting that rather reduced EVD in unstressed nexn−/− embryos was influencing heart rate than vice versa.
Although strongly expressed in skeletal muscle, Nexn deficiency appeared not to have severe effects on skeletal muscle organization, function or motility in unstressed nexn−/− embryos. As shown in previous studies, we were able to provoke sarcomeric disarray and impaired skeletal muscle function in nexn−/− embryos by increasing their workload6. Interestingly, this effect was exclusively seen in cranial regions but not in more caudal areas of the skeletal muscle of the trunk. The cranial somites are the first ones to form during development and thus are the oldest sarcomeres. Together with the fact that this effect can only be recognized under stressed conditions, it implies that skeletal muscle disorganization may be progressive as observed in other diseases affecting the skeletal muscle such as Duchenne Muscular Dystrophy23. Nevertheless, this effect did not seem to be severe enough to impair the motility of the nexn−/− embryos and also patients, to the best of our knowledge, did not report of skeletal muscle involvement6. Whereas NEXN is predominantly described to be involved in sarcomeric stabilization and T-Tubule formation in cardiac tissue, the current knowledge about the role of NEXN in the skeletal muscle includes a role in the glucose uptake of skeletal muscle cells via regulation of insulin receptor substrate 1-signaling24. This could be a hint that NEXN is having differing functions in heart and skeletal muscle as it is already described for other Z-disk proteins such as α-actinin-225,26.
It is remarkable that CRISPR/Cas9-generated nexn knockout embryos in contrast to MO-induced nexn knockdown embryos show a milder cardiac phenotype without a pronounced pericardial edema and blood congestion. This phenomenon has already been observed in previous studies regarding other CRISPR/Cas9-mediated gene knockouts and could be induced by NMD which seems to be not present in MO-induced knockdowns15,27,28. Briefly, the degradation of mutated mRNA by NMD triggers the transcriptional adaptation of a gene or several genes to potentially compensate for the protein loss and suppress or attenuate the phenotype, a process called genetic compensation. The lacking NMD-mediated degradation of nexn mRNA in the CRISPR/Cas9-mediated nexn−/− embryos opposes this mechanism. Nevertheless, recent studies report a similar mechanism called nonsense-mediated translational repression. This mechanism for example seems to get activated after mRNA degradation failure or if certain complexes needed for NMD cannot be recruited and does not lead to altered mRNA levels29,30. As Nexn is a F-actin binding protein, we would suppose another protein with functional homology being upregulated and at least partially compensating for the loss of Nexn. Indeed, by profiling the transcriptome in CRISPR/Cas9-mediated nexn−/− embryos, we found several genes encoding for important sarcomeric proteins being upregulated in nexn mutant embryos, including members of the Troponin, Tropomyosin and Myosin family which are known to interact with Actin31 and may, at least to some extent, take over the function of Nexn by stabilizing the sarcomeric Z-disk. Nevertheless, this hypothesis has to be assessed and needs further detailed elucidation.
In summary, we were able to establish and characterize for the first time a homozygous zebrafish model of constitutive Nexn deficiency, enabling the further investigation of the role of nexn on heart and skeletal muscle development and function as well as the possible compensatory mechanisms triggered by mutation of nexn.
Methods
Zebrafish strains and injection procedures
The present study was performed according to institutional approvals (Regierungspräsidium Tübingen No. 1243 and No. 1530, Tierforschungszentrum (TFZ) Ulm University, No. z.183 and o.183-14) coinciding with EU Directive 2010/63/EU. Experiments were in accordance with ARRIVE guidelines and performed according to relevant guidelines and regulations.
Care and breeding of zebrafish, Danio rerio, were conducted as described previously6.
The lines were generated using CRISPR/Cas9 technology. 400 ng/µl Cas9 protein (Euphoria GmbH, Germany) were injected together with 130 ng/µl tracrRNA (Eurofins Genomics, Germany) and 70 ng/µl of gene-specific crRNA (Eurofins Genomics, Germany) against nexn diluted in 200 mM KCl, resulting in the Nexn deficient line nexn−/−. If not stated differently, MO injections were conducted with the TüAB wildtype strain. Sequences of tracrRNA and CRISPR RNA oligonucleotides can be found summarized in Fig. S7 in the Supplementary Materials.
MO (Gene Tools, LLC, Oregon, USA) was directed against a splice site of nexn6. Standard Control (Std Ctrl) MO was used in the respective MO concentration as negative control. Injections were performed during one-cell stage of zebrafish embryos. MO sequences can be found summarized in Fig. S7 in the Supplementary Materials.
Assessment of heart weight in adult fish
Adult nexn+/+ and nexn−/− fish (2 years old) were sacrificed and the heart was extracted. The heart weight was assessed in proportion to the body weight and the heart weight/body weight ratio calculated.
Birefringence analysis and heart and skeletal muscle functional assessment
Images were taken with a Zeiss Axio Zoom V.16 microscope and movies were recorded with a Leica DM IL LED microscope. Cardiac contractility was analyzed by assessing ventricular FS at 48, 72 and 120 hpf as described previously32. Instead of the diameter of the ventricle, the diastolic and systolic lumen were measured. EVD was assessed with ImageJ.
Birefringence analysis was performed as previously described15.
To assess the responsiveness to mechanical stimuli, zebrafish embryos were touched with the tip of a needle at 48, 72 and 120 hpf. A straightforward flight response was considered as adequate. For detailed analysis, the velocity and acceleration of the embryos were calculated. Briefly, the embryo was put into the center of a petri dish and touched with the needle. Flight response was recorded with 60 frames per second and the video was analyzed using Tracker Video Analysis and Modeling Tool open-source software (physlets.org, accessed on June 07th 2023).
Stressing the embryos was performed in two different approaches. To increase the workload on the heart and skeletal muscles, embryos at 48, 72 and 120 hpf, respectively were incubated in 1% methylcellulose for 2 h as previously described15. In a second approach, to increase the workload on the heart, embryos were treated with 10 µM Isoproterenol (Sigma, USA) for 100 min at the stages mentioned above.
Immunoblotting
Protein lysates were gained from 72 hpf nexn+/+ and nexn−/− embryos as previously described33. For immunoblot analysis, 10 µg of protein were denatured in 5 × Laemmli buffer and separated on 8–16% Tris–glycine gels (BIO-RAD, Hercules, CA, USA). Blotting was performed on polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo System (BIO-RAD, USA). Membranes were blocked in SuperBlock (TBS) Blocking Buffer (Thermo Scientific, Waltham, MA, USA) for 15 min at room temperature (RT). Afterwards, they were incubated with primary antibody diluted 1:1000 in SuperBlock at 4 °C overnight (ON). After washing, the membranes were incubated with the corresponding secondary antibody conjugated to horseradish peroxidase (dilution 1:2500 in TBST (Tris-buffered saline, 0.05% Tween 20)) and developed using Pierce ECL Western Blotting Substrate (Thermo Scientific, USA) and a luminescent image analyzer (Image Quant Las4000 mini)33.
Antibodies used: β-Actin (mouse; #A5441; Sigma, Burlington, MA, USA), Nexn (rabbit; designed by Dieter Fürst lab, Bonn, Germany against epitope position 151-581).
RNA extraction and quantitative real-time PCR
For RNA extraction, 25 nexn+/+ and nexn−/− embryos were collected at 72 hpf. Extraction was done with the RNeasy® Mini Kit (Qiagen, Hilden, Germany) according to the provided manual. For cDNA synthesis, Superscript III reverse transcriptase (Life Technologies, Carlsbad, CA, USA) was used to reverse transcribe 1 µg of RNA. Quantitative real-time PCR was performed using SYBR-Green master mix (Roche, Basel, Switzerland) according to standard protocols on a Roche Light Cycler 480 II. Sequences of the used primers can be found in Fig. S7 in the Supplementary Materials.
RNA sequencing and bioinformatic analysis
For RNA sequencing, pooled RNA of 25 embryos of 72 hpf nexn+/+ or nexn−/− was used. Embryos were obtained by incrossing adult nexn+/+ and nexn−/− fish which are offspring of a heterozygous pair and kept in separate tanks for easier handling. Quality control, library preparation and sequencing were performed by Eurofins Germany (Ebersberg, Germany) using the “INVIEW Transcriptome Discover” pipeline. 35 million 150 bp paired reads were sequenced and mapped to the reference genome GRCz11.
During bioinformatic analysis using R, low read counts were removed from the dataset. Differently expressed genes were calculated with the Bioconductor package DESeq2 and annotated with org. Dr.eg.db and AnnotationDbi34,35. The volcano plot was visualized using the package ggplot236. The read counts of selected genes were color-coded in heatmaps using gplots. GSEA was performed using the clusterProfiler package37 and biological processes terms of the GO database38. Single GO-Terms were further analyzed using the gseaplot2 method of the enrichplot package39.
Immunostaining
For sarcomeric Titin staining, nexn+/+ and nexn−/− embryos (48, 72 and 120 hpf) were fixed in 4% paraformaldehyde (PFA) for 20 min at RT and washed in 0.5% Triton X-100 in PBS three times for 5 min. After incubation in primary antibody (Titin T11, 1:500 in 0.5% Triton X-100 in PBS; Sigma, USA; #T9030) for 1 h at RT, the embryos were washed three times for 5 min in 0.5% Triton X-100 in PBS. Incubation in secondary antibody (mouse IgG2b, 1:1000 in 0.5% Triton X-100 in PBS; Thermo Scientific, USA; #A-21147 ) was performed for 1 h at RT.
For co-staining of MF20 and S46, nexn+/+ and nexn−/− embryos (72) were fixed in Dent’s Fixative (20% DMSO in MeOH) ON at RT. After bleaching in Dent’s Bleach (10% H2O2, 20% DMSO in MeOH) ON at RT, the embryos were rehydrated using a descending MeOH row (75% MeOH, 50% MeOH and 25% MeOH in PBT (PBS + 0.1% Tween)) for 20 min each at RT. After washing 3 times for 2 min in PBDT (1% DMSO, 0.1% Tween in PBS), embryos were blocked using 10% fetal bovine serum (FBS) in PBDT for 1.5 h at 4 °C. Incubation in MF20 antibody (1:10 in 1.5% FBS in PBDT; MF20 concentrate, Developmental Studies Hybridoma Bank, Iowa City, IA, USA) was performed ON at 4 °C. After washing the embryos 4 times for 30 min in 1.5% FBS in PBDT, they were incubated in S46 antibody under the same conditions (S46 concentrate, Developmental Studies Hybridoma Bank, Iowa City, IA, USA). Incubation in the corresponding secondary antibodies (goat anti-mouse IgG1 Alexa Fluor 488 and Alexa 555 goat anti-mouse IgG2b (both Invitrogen, Waltham, MA, USA; #A-21121 and #A-21147)) was performed in 1.5% FBS in PBDT for 3 h at RT.
Images were acquired using a Leica DMi8 microscope (Leica Mikrosysteme Vertrieb GmbH, Wetzlar, Germany).
In situ hybridization
In situ hybridization was performed essentially according to previous publications using a full-length digoxigenin-labeled nexn antisense probe40.
Statistical analysis
Statistical analysis was performed using GraphPad Prism9. qRT-PCR results were analyzed on the mean ΔCT-values using Wilcoxon matched-pairs signed-rank test. The same test was used for Western Blot analysis. All other statistical analyses were performed either using Mann–Whitney test or t-test as stated in the corresponding figure legend with statistical significance at p < 0.05. All results are expressed as mean ± standard (SD) deviation.
Data availability
The dataset generated and analyzed during the current study is available in the Gene Expression Omnibus (GEO) repository (accession number GSE239788).
References
World Health Organization. Cardiovascular diseases. https://www.who.int/health-topics/cardiovascular-diseases#tab=tab_1 (2023).
Ciarambino, T., Menna, G., Sansone, G. & Giordano, M. Cardiomyopathies: An overview. Int. J. Mol. Sci. 22, 7722 (2021).
Richardson, P. et al. Report of the 1995 World Health Organization/International Society and Federation of Cardiology Task Force on the Definition and Classification of cardiomyopathies. Circulation 93, 841–842 (1996).
Japp, A. G., Gulati, A., Cook, S. A., Cowie, M. R. & Prasad, S. K. The diagnosis and evaluation of dilated cardiomyopathy. J. Am. Coll. Cardiol. 67, 2996–3010 (2016).
McNally, E. M., Golbus, J. R. & Puckelwartz, M. J. Genetic mutations and mechanisms in dilated cardiomyopathy. J. Clin. Investig. 123, 19–26 (2013).
Hassel, D. et al. Nexilin mutations destabilize cardiac Z-disks and lead to dilated cardiomyopathy. Nat. Med. 15, 1281–1288 (2009).
Ohtsuka, T. et al. Nexilin: A novel actin filament-binding protein localized at cell-matrix adherens junction. J. Cell Biol. 143, 1227–1238 (1998).
Li, H. et al. Quantification of biventricular myocardial strain using CMR feature tracking: Reproducibility in small animals. BioMed Res. Int. 2021, 8492705 (2021).
Li, H. et al. Feasibility of real-time cardiac MRI in mice using tiny golden angle radial sparse. NMR Biomed. 33, e4300 (2020).
Johansson, J. et al. Loss of Nexilin function leads to a recessive lethal fetal cardiomyopathy characterized by cardiomegaly and endocardial fibroelastosis. Am. J. Med. Genet. A 188, 1676–1687 (2022).
Liu, C. et al. Nexilin is a new component of junctional membrane complexes required for cardiac T-tubule formation. Circulation 140, 55–66 (2019).
Bruyndonckx, L. et al. Childhood onset nexilin dilated cardiomyopathy: A heterozygous and a homozygous case. Am. J. Med. Genet. A 185, 2464–2470 (2021).
Aherrahrou, Z. et al. Knock-out of nexilin in mice leads to dilated cardiomyopathy and endomyocardial fibroelastosis. Basic Res. Cardiol. 111, 6 (2016).
Ruparelia, A. A., Oorschot, V., Vaz, R., Ramm, G. & Bryson-Richardson, R. J. Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Acta Neuropathol. 128, 821–833 (2014).
Diofano, F. et al. Genetic compensation prevents myopathy and heart failure in an in vivo model of Bag3 deficiency. PLoS Genet. 16, e1009088 (2020).
Kossack, M. et al. Induction of cardiac dysfunction in developing and adult zebrafish by chronic isoproterenol stimulation. J. Mol. Cell. Cardiol. 108, 95–105 (2017).
Caviedes Bottner, P., Córdova Fernández, T., Larraín Valenzuela, M. & Cruces Romero Presentación de Casos Clínicos, P. Miocardiopatía dilatada e insuficiencia cardíaca grave. Puesta al día para el médico pediatra. Archivos argentinos de pediatria 116, e421–e428 (2018).
Poudel, K. et al. Association of serum calcium and phosphorus with measures of left ventricular structure and function: The ARIC study. Nutr. Metab. Cardiovasc. Dis. NMCD 30, 758–767 (2020).
Vincent, J.-L. Understanding cardiac output. Crit. Care 12, 174 (2008).
Kenny, J., Plappert, T., Doubilet, P., Salzman, D. & Sutton, M. G. Effects of heart rate on ventricular size, stroke volume, and output in the normal human fetus: A prospective Doppler echocardiographic study. Circulation 76, 52–58 (1987).
de Simone, G. et al. Relation of heart rate to left ventricular dimensions in normotensive, normal-weight children, adolescents and adults. Ital. Heart J. 2, 599–604 (2001).
Adorisio, R. et al. Heart rate reduction as a marker to optimize carvedilol treatment and enhance myocardial recovery in pediatric dilated cardiomyopathy. Front. Physiol. 13, 1001752 (2022).
Annexstad, E. J., Lund-Petersen, I. & Rasmussen, M. Duchenne muscular dystrophy. Tidsskrift for den Norske laegeforening: tidsskrift for praktisk medicin, ny raekke 134, 1361–1364 (2014).
Lee, A., Hakuno, F., Northcott, P., Pessin, J. E. & Rozakis Adcock, M. Nexilin, a cardiomyopathy-associated F-actin binding protein, binds and regulates IRS1 signaling in skeletal muscle cells. PLoS One 8, e55634 (2013).
Bendig, G. et al. Integrin-linked kinase, a novel component of the cardiac mechanical stretch sensor, controls contractility in the zebrafish heart. Genes Dev. 20, 2361–2372 (2006).
Mohapatra, B. et al. Mutations in the muscle LIM protein and alpha-actinin-2 genes in dilated cardiomyopathy and endocardial fibroelastosis. Mol. Genet. Metab. 80, 207–215 (2003).
El-Brolosy, M. A. et al. Genetic compensation triggered by mutant mRNA degradation. Nature 568, 193–197 (2019).
Ma, Z. et al. PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. Nature 568, 259–263 (2019).
Lee, H. C., Oh, N., Cho, H., Choe, J. & Kim, Y. K. Nonsense-mediated translational repression involves exon junction complex downstream of premature translation termination codon. FEBS Lett. 584, 795–800 (2010).
You, K. T. et al. Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. PLoS Biol. 5, e109 (2007).
Gordon, A. M., Homsher, E. & Regnier, M. Regulation of contraction in striated muscle. Physiol. Rev. 80, 853–924 (2000).
Hoage, T., Ding, Y. & Xu, X. Quantifying cardiac functions in embryonic and adult zebrafish. Methods Mol. Biol. 843, 11–20 (2012).
Hirth, S. et al. Paxillin and focal adhesion kinase (FAK) regulate cardiac contractility in the zebrafish heart. PLoS One 11, e0150323 (2016).
Carlson, M. org.Dr.eg.db: Genome wide annotation for Zebrafish. R package version 3.8.2. (Bioconductor, 2019).
Pagès, H., Carlson, M., Falcon, S. & Li, N. AnnotationDbi (Bioconductor, 2017).
Wickham, H. ggplot2: Elegant Graphics for Data Analysis (Springer, 2009).
Wu, T. et al. clusterProfiler 4.0: A universal enrichment tool for interpreting omics data. Innovation 2, 100141 (2021).
Ashburner, M. et al. Gene ontology: Tool for the unification of biology The Gene Ontology Consortium. Nat. Genet. 25, 25–29 (2000).
Yu, G. enrichplot (Bioconductor, 2018).
Jowett, T. & Lettice, L. Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes. Trends Genet. TIG 10, 73–74 (1994).
Acknowledgements
We would like to thank Regine Baur, Renate Durst, Karin Strele, Katrin Vogt, Sabrina Diebold, Jessica Hofmiller and Denise Mann for their excellent technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) JU2859/7-1 and JU2859/9-1 (SJ) and the German Federal Ministry of Education and Research (BMBF) (e:Med-SYMBOL-HF grant #01ZX1407A; e:Med-coNfirm grant #01ZX1708C) (SJ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding
Open Access funding enabled and organized by Projekt DEAL.
Author information
Authors and Affiliations
Contributions
J.H.: Conceptualization, Methodology, Formal analysis, Investigation, Writing, Visualization; B.M.G.: Investigation, Writing; M.H.: Investigation; A.B.: Data analysis, Visualization; W.R.: Supervision; S.J.: Resources; Writing, Supervision, Project administration, Funding acquisition.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Hofeichner, J., Gahr, B.M., Huber, M. et al. CRISPR/Cas9-mediated nexilin deficiency interferes with cardiac contractile function in zebrafish in vivo. Sci Rep 13, 22679 (2023). https://doi.org/10.1038/s41598-023-50065-9
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-023-50065-9
- Springer Nature Limited
This article is cited by
-
Nexilin in cardiomyopathy: unveiling its diverse roles with special focus on endocardial fibroelastosis
Heart Failure Reviews (2024)