Abstract
Insect-pollinated plants often release complex mixtures of floral scents to attract their pollinators. Yet scent compounds eliciting physiological or behavioural responses in pollinators have only been identified in few plant species. The sapromyiophilous aroid Arum maculatum releases a highly diverse dung-like scent with overall more than 300 different compounds recorded so far to attract its psychodid and other fly pollinators. The volatiles’ role in pollinator attraction is mostly unknown. To identify potential behaviourally active compounds, we recorded electroantennographic responses of four Psychodidae and one Sphaeroceridae species to (1) inflorescence scents of A. maculatum and (2) the scents released by cow dung, likely imitated by the plant species. Here we show that these flies are sensitive to 78 floral volatiles of various chemical classes, 18 of which were also found in cow dung. Our study, which for the first time determined physiologically active compounds in the antennae of Psychoda spp. and Sphaeroceridae, identified various volatiles not known to be biologically active in any floral visitors so far. The obtained results help deciphering the chemical basis that enables A. maculatum and other plants, pollinated by psychodids and sphaerocerids, to attract and deceive their pollinators.
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Introduction
Floral scents are important mediators of plant–animal interactions1. Typically, a floral scent bouquet is composed of 20–60 volatiles per plant species2, but some species emit highly complex bouquets consisting of more than 100 volatiles3,4,5. In generalist pollination systems, widespread compounds (e.g., 2-phenylethanol, phenylacetaldehyde, benzaldehyde6,7,8) are typically involved in pollinator attractions, whereas in specialised systems9, the specificity in pollinator attraction is often reached by the emission of either specific blends composed of common compounds10 or highly specific compounds. Examples of such particular compounds are diacetin, which attracts highly specialised oil-collecting bees11,12, 4-methyl-5-vinylthiazole, which attracts cyclocephaline beetles13,14,15, or p-cresol as well as dimethyl trisulphide, which attract flies seeking oviposition sites16,17. Although the number of compounds known to be involved in pollinator attraction is increasing, the biological role of the more than 2,000 floral volatile organic compounds (VOCs) that have already been described2,18 is largely unknown.
In plant species with highly complex bouquets, it is often not possible to obtain all floral compounds as pure substances and to disentangle their individual behavioural effects. Additionally, many scent compounds are often not identifiable because characterised references are missing and are then listed as unknowns, which complicates investigations of their biological role4,5. In consequence, there are large gaps in our understanding of the chemical communication, especially between plants with complex scents and their pollinators.
The fly-pollinated and brood-site deceptive Arum maculatum L. (Araceae, Fig. 1), a widespread European perennial herb, emits a remarkably diverse scent. A single individual releases up to 150 different scent compounds5, and overall more than 300 compounds have been recorded for this plant species so far5,19,20,21,22,23. However, most of these compounds have not yet been identified5. Among the identified compounds, some are widespread floral scents (e.g., methyl salicylate, germacrene D) and/or well-known from brood-site deceptive plants (e.g., indole, p-cresol, 1-octen-3-ol)16, while others are rarely found as floral scent compounds (e.g., β-lutidine, p-cresyl butyrate)2,18.
In Central and much of Western Europe, A. maculatum is predominantly visited and pollinated by female Psychoda phalaenoides L. (Psychodidae, Diptera)24,25. Yet, in Western France and the Mediterranean region, the most abundant visitors are often other psychodid species (e.g., P. grisescens tonn., P. trinodulosa tonn., P. zetterstedti ježek) as well as non-psychodid Diptera, such as Sphaeroceridae24,26,27. Psychodidae and Sphaeroceridae are rare pollinators of angiosperms, but apart from A. maculatum also pollinate other species of Arum (e.g., A. concinnatum, A. cylindraceum, A. cyrenaicum, A. italicum,) and other species/genera of Araceae (e.g., Typhonium eliosurum, Arisaema heterocephalum)28,29,30,31,32. In A. maculatum, insects are attracted on the first day of anthesis (female stage) to the pitfall-trap inflorescence (Fig. 1). They slip and fall into the floral chamber (i.e., the lower part of the inflorescence where the fertile flowers are situated), where they are trapped overnight and are only released on the second and final day of flowering, after being dusted with fresh pollen19,32,33.
The strong, dung-like inflorescence scent of A. maculatum attracts its fly-pollinators21,34 which breed in (and mate on) a range of different decaying organic matter, such as moist leaf litter, mushrooms, cow or horse dung35,36,37,38. Although the pollination biology of A. maculatum is well-studied31,32,33, the role of the individual floral volatiles for pollinator attraction remains largely unknown. To date, only five compounds of A. maculatum (i.e., indole, p-cresol, 2-heptanone, α-humulene, and skatole), all also released by cow dung39,40, have been tested for attractiveness to potential pollinators, and their attractive function in (female) Psychodidae has been proven28,34,41. However, preliminary bioassays at two natural sites of A. maculatum (Salzburg/Austria; Daone/North Italy) could not verify that these compounds, alone or as a blend, are attractive to psychodid (and sphaerocerid) flies, suggesting that other compounds are more likely to be responsible for the main attractiveness of the inflorescence (Gfrerer et al., unpubl.).
Compounds potentially behaviourally active in plants with complex scents, such as A. maculatum, can be pre-selected by measuring the insects’ peripheral olfactory detection of floral scents, using gas chromatography coupled to electroantennographic detection (GC–EAD)42,43. In previous studies testing scent of various plant species (e.g., Ceropegia sandersonii44, Encephalartos villosus45), such measurements frequently pointed to candidate attractants and subsequently allowed the identification of behaviourally active compounds of (complex) scent blends44,45,46. A comparison of the physiologically active floral compounds with the physiologically active compounds of the imitated substrate (or element) also helped to identify the key compounds of the plant–pollinator interactions47,48. Here, we investigated the antennal responses of four psychodid and one sphaerocerid species to the inflorescence scents of A. maculatum and to scent released by cow dung. Specifically, we asked (1) which of the many compounds recorded from this plant species elicit antennal responses in inflorescence visitors, (2) whether antennal responses differ among insect species, and between sexes within species, and (3) how many of the EAD-active scent compounds are shared between A. maculatum and cow dung. The study overall aimed to identify potential scent candidates responsible for attracting and deceiving different pollinators of A. maculatum.
Material and methods
Insect sampling
During 2017–2020, we obtained insects at two natural sites of A. maculatum, one located in Salzburg (Austria, 47°46′59"N 13°04′30"E) and the other in Marktschellenberg (Germany, 47°41′05"N 13°03′30"E). In 2017 and 2018, containers (5 L microboxes, Model: TP5000 + TPD5000–18.5 cm × 18.5 cm × 19.1 cm; Combiness nv, Nevele, Belgium) filled with fresh cow dung (c. 2 L) were offered to insects for three consecutive days as oviposition substrate at the Salzburg site (April–October). Afterwards, the containers were brought to the lab. Once psychodids and sphaerocerids started to hatch in the boxes, the flies were transferred to a small outdoor flight cage (60 cm × 60 cm × 60 cm; BugDorm, Talchung, Taiwan), in which they were offered different breeding substrates (soil, leaf litter, mushrooms, cow or horse dung). Insects reproduced in the cage and were available for electrophysiological measurements for four to six weeks, depending on the species. In spring 2019 and 2020, all insects used for measurements were obtained by bagging A. maculatum individuals with mesh bags at both sites in the morning of the second day of anthesis, prior to the release of trapped insect visitors. Once released by the plant and trapped in the bag, the flies were transferred to a flight cage and bred as described above.
Floral scent collection, electrophysiological analyses (GC-EAD), and identification of EAD-active compounds
To acquire solvent scent samples for electroantennographic analyses, we collected inflorescence scent of A. maculatum and volatiles released by cow dung using dynamic headspace methods, following44,49. Plant volatile samples were collected from a total of eight populations (see Supplementary information Table S1), covering most of the observed scent diversity of A. maculatum5, whereas dung volatiles were obtained from cow dung samples (fresh or 1-day old) used for the rearing of flies (see above). Each inflorescence was enclosed in a plastic oven bag (c. 30 cm × 12 cm; Toppits, Melitta, Germany) on the first day of anthesis between 17:30 and 20:00, when scent emission is strongest5,22. Circa 60 mL dung was placed into 250 mL glass jars, covered with a plastic oven bag (see above). Volatiles were collected on adsorbent tubes (length: 8 cm, diameter: 2 mm), filled with a mixture of Tenax-TA (mesh 60–80) and Carbotrap B (mesh 20–40; 10 mg each; both Supelco, Germany), that were inserted through small holes into the headspace of the inflorescence and dung each. Samples were collected for 0.5–1.5 h with a flow of 100 mL min−1, generated by a battery-operated vacuum pump (rotary vane pump G12/01 EB, Gardner Denver Austria GmbH, Vienna, Austria). Due to the thermogenic activity of the inflorescence22,50, we partly opened the plastic oven bag at the top to avoid strong condensation of water inside the bag. Samples were eluted from each adsorbent tube using 80–100 µL acetone (SupraSolv, Merck KgaA, Germany; following49,51). Then, samples were pooled per population (plant) or per age (dung, i.e., fresh or 1-day-old) to be used for the physiological measurements. To confirm physiological responses to specific compounds, we recorded antennal responses to mixtures of synthetic compounds for a subset of compounds found in A. maculatum (Table 1, Supplementary information Table S1).
Electrophysiological measurements were performed with a gas chromatograph (Agilent 7890A, Santa Clara, California, USA) equipped with a flame ionization detector (FID) and an electroantennographic detection system (GC–EAD)44,49. One microliter of a solvent scent sample was injected in splitless mode (250 °C), with hydrogen as the carrier gas (column flow: 3 mL min−1). During the period of testing (2017–2020), the GC was equipped with three different columns. In 2017, it was a ZB-5 fused silica column (5% phenyl polysiloxane; 30 × 0.32 mm, 0.25 µm film thickness; Phenomenex, Torrance, CA, USA), which was replaced in 2018 by a chiral fused silica capillary (30 m × 0.23 mm I.D.), coated with a 0.23 μm film of 0.4% heptakis (2,3-di-O-methyl-6-O-tert-butyldimethylsilyl)-β-cyclodextrin (DIME-β-CD) (30%) in SE-52 (70%), the same as described in52 and53. From mid-2019 onwards, the GC was equipped with another DIME-β-CD chiral column (MEGA-DEX DMT Beta SE, 30 m × 0.25 mm ID, 0.23 µm film thickness, MEGA S.r.l., Legnano, Italy). The end of each column was split into two capillaries by a μFlow splitter (Gerstel, Mühlheim, Germany), with nitrogen (N2) as make-up gas (flow rate 25 mL min−1). One of the capillaries (2 m × 0.15 µm inner diameter) led to the FID and the other (1 m × 0.2 µm inner diameter) to the EAD setup. The EAD was set up by a transfer line, heated at 220 °C, and a 2-channel USB acquisition controller (Syntech, Kirchzarten, Germany). The outlet of the EAD was placed in a cleaned, humidified airflow, directed onto the mounted antenna. Prior to measurements, each fly was anaesthetised with CO2, and the head and last antennomere (apical-tip) were removed. Subsequently, the head and one randomly selected antenna were each connected to a glass micropipette electrode, filled with 95% insect Ringer’s solution (8.0 g L−1 NaCl, 0.4 g L−1 KCl, 4.0 g L−1 CaCl2) and 5% Tween 20 (Sigma Aldrich, Vienna, Austria), and connected to silver wires. The recording electrode was attached to the tip of the antenna, while the reference electrode was connected to the caudal side of the head44,54.
Solvent scent samples of A. maculatum were tested on the antennae of five Diptera species: the Sphaeroceridae Coproica ferruginata stenh. (one female) and four Psychodidae species, i.e., Psychoda phalaenoides (12 females and eight males), P. zetterstedti (two females and two males), P. trinodulosa (one male), and P. cinerea banks (two females) (Table 1, Supplementary information Table S1). All these fly species are visitors of A. maculatum24,25;27, except for P. cinerea, which is a pollinator of A. hygrophilum and A. italicum29, with the latter sharing several floral compounds with A. maculatum21,32. Two additional female Psychoda individuals (collected directly from A. maculatum) could not be determined to species level, as their abdomens were too damaged, and might belong to the four above mentioned species or (an)other species.
For identification of EAD-active compounds, scent samples were run on a gas chromatograph/mass spectrometer (GC/MS, model QP2010 Ultra EI, Shimadzu, Tokyo, Japan), equipped with either a non-chiral ZB-5 column (in 2017; see above) or a chiral column (2018–2020; MEGA-DEX DMT Beta SE, see above). Helium was used as carrier gas (flow: 3 mL min−1) and samples (injection volume: 1 µL) were run with a split ratio of 1:144,49. Obtained data were handled using GCMSolution v.4.41 (Shimadzu Corporation, Kyoto, Japan). We tentatively identified components by comparison of Kováts’ retention indices55 (KRIs; based on commercially available n-alkanes C7–C20) and mass spectra available in the libraries of Adams56, FFNSC 2, Wiley9, NIST11, and ESSENTIAL OILS (available in MassFinder 3, Hochmuth Scientific Consulting, Hamburg, Germany). The identity of some of the components was verified by authentic reference standards, available in the collection of the Plant Ecology Lab of Salzburg University (see Table S1). Compounds were classified as inflorescence-specific or as vegetative compounds, according to Gfrerer et al.5. Absolute amounts of compounds tested in the GC/EAD measurements (Table 1) were quantified by injecting known amounts of various aliphatics and terpenoids and the resulting mean peak areas were used for quantification5.
Results
Across all tested dipterans, we found a total of 78 volatile organic compounds (VOCs) from A. maculatum (together c. 88% of the relative inflorescence scent emission of this species5) that were electroantennographically active. The majority of these compounds were inflorescence-specific, but five of them were vegetative compounds [i.e., (Z)-3-hexenyl acetate, nonanal, limonene, linalool, α-pinene; Table 1]. Overall, 55 of the EAD-active VOCs could be (tentatively) identified (Table 1). They represented several chemical classes, including monoterpenoids (n = 25 VOCs), aliphatic compounds (15), sesquiterpenoids (eight), irregular terpenoids (two), nitrogen-bearing compounds (three), and aromatic compounds (two). Seven of these VOCs elicited antennal responses in all tested insect species: the monoterpenoid 2,6-dimethylocta-2,6-diene (isomer 2), the aliphatic compound 1-octen-3-ol, the sesquiterpene (E)-β-caryophyllene, the nitrogen-containing component indole, the aromatic components methyl salicylate and p-cresol, and the unknown UNK1415 (Table 1, Fig. 2). All other VOCs elicited responses only in a subset of insect species.
Representative physiological responses (gas chromatography coupled to electroantennographic detection) of a female Psychoda sp. (Psychodidae; red) and a female Coproica ferruginata (Sphaeroceridae; violet) to scent samples of (A) inflorescences of Arum maculatum and (B) cow dung (fresh and 1-day old). EAD-active compounds (see also Table 1): (1) UNK883; (2) hexanal; (3) 1-hexanol; (4) 3,7-dimethyloct-1-ene; (5) ( +)-α-citronellene; (6) ( +)-β-citronellene; (7) 3,7-dimethyl-2-octene isomer 1; (8) 3,7-dimethyl-2-octene isomer 2; (9) 2,6-dimethyl-2,6-octadiene isomer 2; (10) 6-methyl-5-hepten-2-one; (11) UNK1030; (12) octanal; (13) p-cymene; (14) 6-methyl-5-heptene-2-ol; (15) 1-octen-3-ol; (16) 2-methylbutanoic acid; (17) 2-nonanone; (18) nonanal; (19) 2,6-dimethyl-7-octen-2-ol; (20) (E)-2-octen-1-ol; (21) (S)-linalool; (22) 2-decanone; (23) UNK1135; (24) decanal; (25) p-cresol; (26) 3,7-dimethyl-1-octanol; (27) α-copaene; (28) UNK1378/81; (29) UNK1394; (30) UNK1415; (31) indole; (32) UNK1503/UNK1492; (33) UNK 1658. Numbers in bold (top line) are VOCs eliciting signals in (A) and (B); numbers in italics (bottom line) are VOCs that do not occur in A. maculatum. FID1 A. maculatum, population Josefiau; FID2 A. maculatum, population Murnau; FID3 cow dung fresh; FID4 cow dung 1-day-old. See Supplementary Table S1 for detailed population information. All samples shown were run on a chiral fused silica capillary column (30% DIME-β-CD in 70% SE-52, see methods) and measurements lasted either 8 (Coproica ferruginata) or 10 min (Psychoda sp.).
On average, 38 scent compounds yielded a response per species (and sex), with a minimum of 13 volatiles in male P. trinodulosa (Psychodidae; one individual tested on one scent sample), and a maximum of 60 VOCs in female P. phalaenoides (12 individuals tested on nine scent samples). When considering both sexes of P. phalaenoides (total of 20 individuals tested on 16 scent samples), 61 VOCs were EAD-active, and in both sexes of P. zetterstedti 49 VOCs (four individuals on eight scent samples; Table 1, Supplementary information Table S1). For the female C. ferruginata (Sphaeroceridae; one individual tested on three samples), 36 VOCs resulted in an antennal response. Notably, the aliphatic compound 2-nonanone was perceived by all psychodid species, but did not elicit signals in C. ferruginata, even though this compound was present in all three scent samples tested on the latter species. In contrast, the monoterpene γ-terpinene was EAD-active only in C. ferruginata, but not in the two psychodid species tested on this compound, i.e., P. phalaenoides and P. sp.
Some compounds elicited specific responses in certain Psychoda taxa. For instance, the aliphatic compound nonanal induced responses in P. phalaenoides and P. zetterstedti, but not in P. cinerea and P. trinodulosa. The nitrogen-bearing compound skatole was EAD-active in P. phalaenoides and P. cinerea, but not in P. zetterstedti and P. sp. (Table 1). The sesquiterpene α-humulene resulted in responses in most individuals of P. zetterstedti and in a few individuals of P. phalaenoides, but not in P. cinerea and P. sp. A number of the unknown volatiles (e.g., UNK883, UNK1394, UNK1492) elicited responses in P. phalaenoides and P. zetterstedti, but not in P. cinerea.
For P. phalaenoides and P. zetterstedti, the two species for which we tested male and female individuals, the analyses revealed some sex-specific responses. In P. phalaenoides, 24 compounds were EAD-active in both sexes, but one compound elicited responses only in females (unknown UNK1030; n = 12 individuals). In P. zetterstedti, 21 compounds were EAD-active in both sexes, but four compounds [2,6-dimethylocta-2,6-diene isomer 1, (Z)-β-ocimene, α-copaene, 6-methyl-5-hepten-2-one] and two [( +)-α-citronellene, unknown UNK1378/81] elicited responses only in females (n = 2) and males (n = 2), respectively.
Of the 78 EAD-active floral compounds, 18 were also physiologically active in cow dung samples (Fig. 2, Table 1), representing all different chemical classes recorded in A. maculatum. More specifically, six of the seven VOCs that elicited antennal responses in all tested insect species were among those 18 compounds [i.e., 2,6-dimethylocta-2,6-diene isomer 1, 1-octen-3-ol, (E)-β-caryophyllene, indole, p-cresol, and UNK1415; Fig. 2]. Among those 18 were also VOCs that elicited different responses among fly families (2-nonanone), fly species (nonanal, skatole), and between sexes within species [i.e., 2,6-dimethylocta-2,6-diene isomer 1, 6-methyl-5-hepten-2-one, (E)-β-caryophyllene].
Discussion
Our study is the first to identify electroantennographically active compounds in Psychoda spp. (Psychodidae) and a Sphaeroceridae (Coproica ferruginata). It shows that these insect visitors of deceptive Arum maculatum are sensitive to a high number of the plants’ inflorescence scent compounds. The EAD-active compounds identified represent various chemical classes, including mono- and sesquiterpenoids, aliphatic, aromatic, nitrogen-bearing, and unknown compounds. Antennal responses differed among insect species and between sexes within species. More than a fifth of the physiologically active scent compounds were also released by cow dung, linking insect breeding/mating sites, floral VOCs of A. maculatum, and its floral visitors.
A few of the compounds recorded as physiologically active in the antennae of Psychoda spp. (1-octen-3-ol, butanoic acid, α-pinene, and α-terpinene) were also found to elicit electrophysiological responses in females of the phlebotomine sandfly Lutzomyia longipalpis (Psychodidae, Diptera)56,57, the only other psychodid used so far for physiological measurements in the olfactory circuitry. This sandfly, which was tested on faeces from vertebrates and canid host odours, additionally responded to several other volatiles that do not occur in A. maculatum (including different isomers of monoterpenoids), but we provide the first evidence that psychodids are able to perceive sesquiterpenoids. Physiological measurements on antennae of Sphaeroceridae were not available before our measurements, and thus overall, our study increases the knowledge about the peripheral olfactory circuitry of psychodids and Sphaeroceridae.
Among the EAD-active volatiles recorded in the present study are the most abundant inflorescence scents of A. maculatum included (i.e., indole, p-cresol, ( +)-β-citronellene, 2-heptanone, 3,7-dimethyloct-2-ene, UNK14155,19,21,22,23,32), but also numerous compounds emitted only in small relative amounts by the plant (e.g., 3-octanone, 2,6-dimethyl-7-octen-2-ol; Fig. 2). Several of these abundant and less abundant compounds are also released from cow dung (Table 1, Fig. 2) or other breeding/mating sites of the tested insect species. These are, for instance, p-cresol, 2-heptanone, terpinen-4-ol, α-citronellene, and 2,6-dimethylocta-2,6-diene (cow dung; Table 140), 2-octanone (horse dung39), β-citronellene and α-humulene (both cow and horse dung39,40,59), or 3-octanone and (E)-2-octen-1-ol (fungi60). Some of the EAD-active compounds have not been detected in ours or others’ scent samples from (cow) dung and have not been described elsewhere. We speculate that those volatiles are released from various other, likely differently scented breeding and/or mating substrates of A. maculatum pollinators, such as mud-flats or leaf litter. It has been suggested that the hyperdiverse floral scent of A. maculatum might result from the imitation of various differently scented breeding substrates5. The sensitivity of the tested flies to a variety of different compounds would support this idea, although other possible roles for those volatiles cannot be excluded (e.g., repellence of florivores61, defence against pathogens62).
Altogether five inflorescence scent compounds of A. maculatum have previously been reported as attractive to psychodids. In detail, indole and p-cresol, together with α-humulene or 2-heptanone, were found to attract female P. phalaenoides41 and Psychoda spp.34. In Kite et al.34, the attracted psychodids have not been identified to the species level; hence, it is unknown whether they are flower visitors of A. maculatum or not. A synthetic mixture of skatole, indole, and p-cresol, together with VOCs not occurring in A. maculatum (geranyl acetone, dihydro-, and β-ionone), was shown to attract psychodid and sphaerocerid pollinators of Typhonium eliosurum, a dung-mimicking aroid endemic to Australia28. In preliminary bioassays in the field, we tested the above five compounds, using the same composition and concentration as released by the inflorescences of A. maculatum. Yet those volatiles did not attract psychodid or sphaerocerid flies. This suggests that other, not yet tested scent compounds (additionally) contribute to pollinator attraction in A. maculatum. Potential candidates are other odours known also from cow or horse dung (e.g., 2,6-dimethylocta-2,6-diene, unknown UNK1415; Table 1) or compounds known from other breeding substrates (e.g., fungi: 3-octanone60). The unknown UNK1415, one of the main scent compounds of A. maculatum5, yielded antennal responses in all insect taxa and in nearly all individuals tested in the present study. Interestingly, this unknown volatile is possibly identical to unknown “RI 1531” found in T. eliosurum28, as both volatiles have the same mass spectra (Supplementary information Fig. S1).
Our study shows that some of the antennal responses to scent differ among insect species, and some also between males and females within species. This finding is in agreement with results obtained by physiological measurements in other insects43,63. Some of the species-level effects described in the present study might have been influenced by sex-specific effects, because for some species (P. cinerea and P. trinodulosa) we only tested males or females. Hence, differences in antennal responses among these species need to be interpreted with caution. Nonetheless, species- and sex-specific differences in the peripheral olfactory circuitry of insects can result in different behaviours64,65. Interestingly, antennae of P. cinerea, the only non-pollinating species of A. maculatum (but of other Arum spp.) we tested in this study, did not respond to some abundant compounds emitted by A. maculatum (e.g., UNK1394, UNK1492), which otherwise elicited responses in the other pollinating species tested (e.g., P. phalaenoides and P. zetterstedti). The lack of antennal sensitivity to (some of) those compounds might explain why P. cinerea does not visit A. maculatum, while close relatives including P. phalaenoides and P. zetterstedti are (important) pollinators24,25.
Conclusions
Until now, it was not known which (and how many) volatile compounds of the complex floral scent of Arum maculatum can be perceived by its floral visitors. Our study identified 78 physiologically active compounds from hundreds of potentially behaviourally active VOCs, which is still a rather high number the psychodid and sphaerocerid flies are sensitive to. Our results thus provide a basis for future studies that aim to understand the floral volatiles of A. maculatum involved in the chemical attraction and deception of its pollinators, and which VOCs guide the flies to their breeding/mating substrates. Some of the EAD-active VOCs (4-terpinenol, α-terpinene, 2-heptanol, 2-nonanol, UNK1503) have recently been shown to be under phenotypic selection in A. maculatum5. These compounds and those EAD-active ones shared with the pollinators’ breeding substrates (e.g., UNK1415, 3-octanone) are the most promising candidates for future behavioural assays. As the tested Diptera species (Psychodidae, Sphaeroceridae) are also known pollinators of other (similarly-scented) species of Arum (e.g., A. italicum, A. concinnatum31, 66) as well as other species/genera of Araceae (e.g., Typhonium eliosurum28), our study should also help to elucidate the chemical interactions between these plants and their fly pollinators. Future research is now needed to test the behavioural function of physiologically active floral volatiles, which is crucial for a better understanding of olfactory cues mediating plant–animal interactions in general, and in sapromyiophilous species, in particular.
Experimental research and field studies on plants
All samplings were carried out in compliance with the current laws of the respective countries.
Data availability
All data that support the findings of this study are included in this published article (and its supplementary information files).
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Acknowledgements
We are grateful to RNDr. Jindřich Roháček for identifying the tested Sphaeroceridae individual to the species level. We would further like to thank Dr. Irmgard Schäffler and Dr. Roman Fuchs for methodological support, Prof. Dr. Robert R. Junker for the microboxes, and family Bruckbauer (Lienbacher farm) for continuously providing breeding substrates for insect rearing. We are also thankful to members of the Plant Ecology research group at the University of Salzburg for their helpful comments on an earlier version of the manuscript, and to two anonymous reviewers for their suggestions that improved the final manuscript. This study was funded by a grant from the Austrian Science Fund (FWF; P30175-B29) to ACH, HPC, and SD (PI).
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S.D., M.G., A.C.H. and H.P.C. conceived the study. S.D., M.G., and E.G. conceptualised the study. E.G. and D.L. collected the scent samples. D.L. and R.W. identified the Psychodidae to species level. E.G. reared the insects, performed all measurements, analysed all data, prepared all figures, and wrote the first draft of the manuscript. All authors contributed to the final manuscript and approved the submitted version.
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Gfrerer, E., Laina, D., Wagner, R. et al. Antennae of psychodid and sphaerocerid flies respond to a high variety of floral scent compounds of deceptive Arum maculatum L.. Sci Rep 12, 5086 (2022). https://doi.org/10.1038/s41598-022-08196-y
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DOI: https://doi.org/10.1038/s41598-022-08196-y
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