Abstract
DDR1 has been linked to schizophrenia (SCZ) and bipolar disorder (BD) in association studies. DDR1 encodes 58 distinct transcripts, which can be translated into five isoforms (DDR1a-e) and are expressed in the brain. However, the transcripts expressed in each brain cell type, their functions and their involvement in SCZ and BD remain unknown. Here, to infer the processes in which DDR1 transcripts are involved, we used transcriptomic data from the human brain dorsolateral prefrontal cortex of healthy controls (N = 936) and performed weighted gene coexpression network analysis followed by enrichment analyses. Then, to explore the involvement of DDR1 transcripts in SCZ (N = 563) and BD (N = 222), we studied the association of coexpression modules with disease and performed differential expression and transcript significance analyses. Some DDR1 transcripts were distributed across five coexpression modules identified in healthy controls (MHC). MHC1 and MHC2 were enriched in the cell cycle and proliferation of astrocytes and OPCs; MHC3 and MHC4 were enriched in oligodendrocyte differentiation and myelination; and MHC5 was enriched in neurons and synaptic transmission. Most of the DDR1 transcripts associated with SCZ and BD pertained to MHC1 and MHC2. Altogether, our results suggest that DDR1 expression might be altered in SCZ and BD via the proliferation of astrocytes and OPCs, suggesting that these processes are relevant in psychiatric disorders.
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Introduction
DDR1 is a pleiotropic membrane-anchored tyrosine kinase receptor that is expressed in multiple tissues, including the brain [1, 2]. DDR1 gene variants were found to be associated with schizophrenia (SCZ) in candidate gene studies [3,4,5]. In addition, genome-wide association studies found associations between DDR1 SNPs and SCZ [6, 7] and bipolar disorder (BD) [8]. However, they did not include the DDR1 locus in the final analyses because it falls inside of a linkage disequilibrium region with the highest SCZ-associated locus; therefore, these results can be found only in the supplementary materials.
In situ hybridization studies in mice demonstrated that DDR1 is expressed in areas of neurogenesis prenatally, while it overlaps with the dynamics of the myelination process during the postnatal period [9]. In the adult mouse brain, DDR1 mRNA expression was first detected in glia [10], and later, single-cell transcriptome profile analysis showed that DDR1 is mainly expressed in cells of the oligodendrocyte lineage and to a lesser extent in neurons and astrocytes, while its expression in microglia and endothelial cells was almost undetectable [11]. In the adult human brain, the presence of DDR1 is also detected in myelin and in the soma of oligodendrocytes, astrocytes and endothelial cells [12].
Alternative splicing of DDR1 produces 58 transcripts, some of which encode one of the five isoforms known to date, named DDR1a-e [13,14,15]. Each isoform may have different functions. For instance, the upregulation of two different transcripts (encoding DDR1a and DDR1b) after irradiation of in vitro cultured astrocytes suggests their involvement in DNA repair, checkpoint signaling pathways and apoptosis [16, 17]. Additionally, DDR1a was shown to promote the migration of leukocytes [18] and cell invasion and adhesion in gliomas [19]. DDR1c mRNA expression in the human brain correlates with the expression of OLIG2 and MAG, two oligodendrocyte protein markers [20], and is upregulated in brain dorsolateral prefrontal cortex (DLPFC) tissue from patients with SCZ compared to healthy controls (HCs) [21].
Studies of DDR1 expression in the human brain are scarce [22], but data from public repositories show that DDR1 expression decreases with age [23]. Studying coexpression networks during human brain development, we previously described that DDR1 was coexpressed mainly with oligodendrocyte-related genes in the postnatal and early adulthood periods (between 0 and 40 years old) and with astrocyte- and type 2 microglia-related genes in the prenatal period and late adulthood ( > 40 years old) [23]. However, a comprehensive description of each DDR1 transcript coexpression network in human brain tissue and, especially, in psychiatric disorders has not yet been performed.
Here, we used bulk expression data from the human DLPFC to (1) build gene coexpression modules and assess the cell type and biological process enrichments for modules containing DDR1 transcripts, (2) evaluate the association of these coexpression modules with SCZ and BD and (3) test the differential expression of DDR1 transcripts in patients with SCZ or BD with respect to HCs.
Materials and methods
Sample data
Data for this publication were obtained from the NIMH Repository and Genomics Resource, a centralized national biorepository for genetic studies of psychiatric disorders. We retrieved the gene and transcript-level counts from DLPFC samples obtained by RNA-Seq by Expectation-Maximization (RSEM) and corresponding clinical information from HCs, patients with SCZ and patients with BD from 6 different collections of the PsychENCODE project: BrainGVEX, BrainSpan, CMC, BipSeq, LIBD and CMC-HBCC. We removed samples with a diagnosis other than SCZ or BD (N = 9) or without age data (N = 23) to ultimately include 936 HCs, 563 patients with SCZ and 222 patients with BD in the analyses. We tested for differences in sex, age of death and ethnicity among diagnostic groups in each collection and in the whole sample. The chi-squared test was used to compare categorical variables, the Mann‒Whitney test was used to compare continuous variables between two different diagnostic groups, and the Kruskal‒Wallis test was used to compare continuous variables among three different diagnostic groups (Table 1). Lowly expressed genes and transcripts were filtered out using the filterByExpr() function of the edgeR package v3.40.1 [24] to retain genes and transcripts with at least 10 counts per million in 70% of the samples of the smallest group, as previously described [25], and counts from 22548 genes and 81950 transcripts per individual remained. Of note, 43 DDR1 transcripts were excluded from the analysis, and 15 were included: ENST00000376567, ENST00000376570, ENST00000376569 and ENST00000418800 encode DDR1a; ENST00000324771, ENST00000376568 and ENST00000452441 encode DDR1b; ENST00000513240 encodes DDR1c; ENST00000376567 encodes DDR1d; ENST00000508312 encodes DDR1e; ENST00000428153, ENST00000460944, ENST00000484556 and ENST00000446312 encode other DDR1 protein isoforms (not DDR1a-e); and ENST00000508472 is a noncoding transcript. We downloaded genotype data (7.5 million variants) for 68 HC samples from the LIBD collection (Table 1). More detailed information about each collection is reported in the Expression data section of the Supplementary Material.
Expression matrix building
Using the readDGE() function of the edgeR package v3.40.1 [24], we built expression matrices from the RSEM files for HCs, patients with SCZ, patients with BD and the whole sample using the gene-level counts of all genes except for DDR1, for which we used the transcript-level counts. Counts were normalized to counts per million using the cpm() function of the edgeR package v3.40.1 [24] and log2 transformed (Fig. 1). We extracted the principal components (PCs) of the whole sample, plotted PC1 versus PC2 and observed a structure driven mainly by collection (Supplementary Fig. 1A). Furthermore, to remove any variance due to unwanted sources of variation (i.e batches and confounding factors), we used the DaMiRseq R package v2.2.0 [26]. Briefly, the whole-sample expression matrix and clinical information were used to create a summarized object, and the object served as input for DaMiR.SV() function to identify the surrogate variables (SVs) needed to explain 95% of the variance. The number of SVs identified was 4, and all of them correlated with known variables (batch, sex, age of death and ethnicity) but not with diagnostic group (Supplementary Fig. 2), which is a requirement for the use of SVs as covariates [26]. We used the four SVs to adjust the expression matrices using the empiricalBayesLM() function of the WGCNA R package v1.72 [27], which uses Empirical bayes-moderated linear regression to remove unwanted variables (Fig. 1). We plotted PC1 versus PC2 for the new adjusted matrices and observed no sign of structure (Supplementary Fig. 1B).
Quantification of DDR1 transcripts
We retrieved the log2 transformed counts per million (log2CPM) values for each DDR1 transcript from the adjusted matrices and calculated the mean expression of each DDR1 transcript independently for HCs, patients with SCZ and patients with BD.
Expression quantitative trait loci analysis
To study whether SNPs previously reported to explain part of the variation in DDR1 mRNA expression levels (expression quantitative trait loci (eQTLs) for DDR1) in the GTEx portal (https://gtexportal.org/) were eQTLs for individual DDR1 transcripts, we gathered the whole list of SNPs, performed quality control and filtered out variants that were in high linkage disequilibrium (R2 > 0.5) using Plink software v1.9. Ultimately, 146 SNP variants remained. We then selected the corresponding normalized expression data from samples with genotype data available (68 HC samples of the LIBD collection) and performed an eQTL analysis using the GenomicTools R package v0.2.9.7 [28].
Gene network construction and module detection
Adjusted matrices for HCs and the whole sample were used to generate corresponding unsigned coexpression networks using the WGCNA R package v1.72 (Fig. 1). We chose a soft-threshold power (β) of 9 for both networks to ensure that they were comparable, which allowed us to achieve a scale-free topology fitting index ≥ 0.80 (Supplementary Fig. 3). We set the minimum module size at 30, selected a deep split of 4 and merged highly correlated modules (r2 > 0.8). The resulting modules, defined as branches of the network dendrogram, were assigned number labels. We labeled those modules containing DDR1 transcripts first, and genes or transcripts that did not correspond to any coexpression module were assigned the label M0. To evaluate the importance of each DDR1 transcript in a module, we calculated their module membership (MM). Briefly, we extracted the principal component of each module, named the module eigengene (ME), which serves as a summary expression measure of the expression profile within a module, and calculated the correlation of the expression of each DDR1 transcript with the ME of the module in which it was located. DDR1 transcripts with MM > 0.4 were considered hub transcripts. To enable comparisons between coexpression networks, labels of the whole-sample network were reassigned to match those of the modules with significant overlap in the HC network using the matchLabels() function of the WGCNA R package v1.72, which calculates the overlap of the labels in the whole-sample and HC networks using Fisher’s exact test and relabels whole-sample modules so that each one receives the same label as the control module that it overlaps most with. The HC network (whose modules are named MHC) was used to infer the biological importance of each DDR1 transcript and to test whether the connectivity of the HC network is preserved in SCZ and BD (Fig. 1). The whole sample network (whose modules are named MWS) was used to study the correlation of the principal component of each module and the expression of each DDR1 transcript with the diagnosis of SCZ and BD (Fig. 1). Additionally, the whole sample network was used to study whether the variance explained by the cell type composition of the samples was completely removed by the SVs when building the network. With this aim, we calculated the cell type proportion estimates of the normalized whole sample matrix by using the brainCells() function of the BRETIGEA R package [29] and studied the correlation between the resulting cell type proportion estimates and the MEs of whole sample network.
Cell-type enrichment analysis and correlation of DDR1 transcripts with cell proportions
We performed a cell type enrichment analysis of each module containing DDR1 transcripts in the HC network using the hyper() function of the hypeR package v1.14.0 [30] (Fig. 1), which performs a hypergeometric test between a reference and input lists of genes. As a reference, we retrieved a list of the top 1000 marker genes from each of the six major brain cell types (neurons, oligodendrocyte precursor cells (OPCs), oligodendrocytes, astrocytes, endothelial cells and microglia) from a meta-analysis of brain cell expression data available in the markers_df_brain() function of the BRETIGEA R package v1.0.3 [29]. The input lists corresponded to the list of the coding genes of each module. P values were adjusted for multiple testing [31], and an adjusted p-value < 0.01 was considered statistically significant. Additionally, to complement the cell type enrichment analysis, we computed the BRETIGEA cell type proportion estimates for the same cell types using the brainCells() function, setting the number of genes to the top 50 cell type-specific genes for each cell type and using the singular value decomposition approach [32]. Then, we explored the linear correlation between each relative cell type proportion estimate and the expression of individual DDR1 transcripts (Fig. 1).
GO enrichment and neighbor analysis of DDR1 transcripts
We performed nonredundant Gene Ontology (GO) enrichment analyses of the biological process, molecular function and cellular components of the modules containing DDR1 transcripts in the HC network using WebGestalt [33] (Fig. 1). All coding genes included in the study (N = 18163) were used as background, since they are the only genes for which GO annotation is available. P values were adjusted for multiple testing [31], and an adjusted p-value < 0.01 was considered statistically significant. Furthermore, to complement the GO enrichment analysis, we exported the topological overlap matrix of each module containing DDR1 transcripts in the HC network, which reflects the relative interconnectedness between a pair of genes [34], into the interface of Cytoscape software to visualize the ten most correlated neighbors of each DDR1 transcript. Nodes were colored based on their biological process as annotated in UniProt [35].
Module preservation
We assessed the preservation of the connectivity of the HC network in SCZ and BD using the modulePreservation() function of the WGCNA R package v1.72 (Fig. 1). The degree of preservation was evaluated by means of the Zsummary, a statistic that assesses whether the connectivity level and pattern of a module in one dataset is preserved in another [27].
Module-trait association and transcript significance
We evaluated the association of each module containing DDR1 transcripts in the whole sample network and disease by testing the correlation between each ME and a categorical variable where HCs were assigned the value 0 and patients (SCZ and BD) were assigned a value of 1 (Fig. 1). The correlation was tested individually for patients with SCZ and patients with BD. To evaluate whether any DDR1 transcript was independently associated with SCZ or BD, we calculated its transcript significance (Fig. 1), defined as the correlation between transcript expression and the categorical variable representing the diagnosis of SCZ or BD. P values were adjusted for multiple testing [31], and an adjusted p-value < 0.05 was considered statistically significant.
Differential expression analysis
We performed a differential transcript expression analysis between patients and HCs. First, we built an expression matrix with the transcript-level counts of all genes using the readDGE() function of the edgeR package v3.40.1 [24], fitted a general linear model for each transcript and extracted the log2 fold change (log2FC) between patients and HCs (Fig. 1). SVs were included as covariates in the model. Later, to test whether each model coefficient differed significantly from zero, we performed a Wald test. The Wald test p values were subjected to independent filtering to increase the detection power [36] and were adjusted for multiple testing [31]. An adjusted p-value < 0.05 was considered statistically significant. The analysis was performed separately for patients with SCZ and patients with BD using the DESeq2 package v1.38.3 [37].
Results
Relative DDR1 transcript expression and eQTLs
The quantification in TPM of each DDR1 transcript included in the study is shown in Supplementary Fig. 4. ENST00000376569 (DDR1a), ENST00000542441 (DDR1b) and ENST00000376568 (DDR1b) were the 3 most highly expressed transcripts. After performing the eQTL analysis in the subsample of HCs (N = 68), we observed that some SNPs were nominally associated with the expression of DDR1 transcripts. However, none of the associations were significant after multiple test correction; therefore, SNPs were not included as covariables in the subsequent analyses. A complete list of the nominal p values for each SNP is shown in Supplementary Table 1.
Biological importance of DDR1 transcripts
We performed WGCNA with data from HCs and detected 27 modules (Supplementary Table 2), five of which contained DDR1 transcripts (MHC1- MHC5). The MM of DDR1 transcripts ranged from 0.19 to 0.72 in absolute values and is shown in Table 2. Of note, five DDR1 transcripts did not show any pattern of coexpression with other genes and were assigned to MHC0. To determine which cells are more likely to express specific DDR1 transcripts, we performed a cell type enrichment analysis of each module. Three modules were enriched in astrocytes; 3 modules, in neurons; 3 modules, in oligodendrocytes; 4 modules, in endothelial cells; 4 modules, in microglia; and 6 modules, in OPCs (Supplementary Table 3). Regarding DDR1-containing modules, MHC1 was enriched in astrocytes (adjP=0.011) and OPCs (adjP=0.011); MHC2 was enriched in OPCs (adjP=1.3E-10); MHC3 was enriched in OPCs (adjP=0.0019) and oligodendrocytes (adjP=2.8E-51); MHC4 was enriched in OPCs (adjP=5.7E-05) and oligodendrocytes (adjP=1.7E-160); and MHC5 was enriched in neurons (adjP=2.3E-146). No module was enriched in microglia or endothelial cells (Table 2).
The correlation between DDR1 transcript expression and the cell type proportion estimates revealed that individual DDR1 transcripts were correlated with the cell type proportion estimates for astrocytes, OPCs, oligodendrocytes and neurons but also microglia and endothelial cells (Supplementary Fig. 5). Of note, the strongest correlations (r2 > 0.4 and adjP<2.2E-16) were found between DDR1 transcripts of MHC2 and astrocytes, OPCs and oligodendrocytes (with the exception of ENST00000484556); of MHC4 and OPCs and oligodendrocytes; and of MHC5 and neurons. DDR1 transcripts in MHC0, which did not show any pattern of coexpression with other genes and thus did not receive any cell type enrichment calculation, correlated with different cell type proportion estimates. ENST00000418800 (DDR1a), ENST00000508312 (DDR1e) and ENST00000446312 correlated mostly with neurons (adjusted p-values of < 2.2E-16, 1.03E-05 and 6.6E-10, respectively), and ENST00000513240 (DDR1c) and ENST00000376575 (DDR1d) correlated mostly with endothelial cells (adjusted p values of 5.6E-06 and 4.4E-07, respectively).
To elucidate the mechanism by which DDR1 transcripts may be involved in these diseases, we performed a GO enrichment analysis of the modules containing DDR1 transcripts. The results of the biological process term enrichment are provided in Table 2, and a complete list of the results is provided in Supplementary Table 4. Briefly, MHC1, MHC2 and MHC3 were enriched in processes that take place during cell division, MHC4 in gliogenesis and axon ensheathment, and MHC5 in synaptic signaling. To complement the GO enrichment analysis, we visualized the ten more associated neighbors of each DDR1 transcript with Cytoscape and observed that a large proportion of them are involved in the cell cycle and processes such as regulation of transcription, chromatin remodeling and regulation of cell morphology (MHC1, MHC2, MHC3); oligodendrocyte differentiation and myelination (MHC4); and synaptic transmission (MHC5) (Fig. 2).
DDR1 transcript module preservation and association with SCZ and BD
We assessed whether there was any gene coexpression disruption in the SCZ and BD networks with respect to the HC network by calculating the Zsummary of each module containing DDR1 transcripts. All modules containing DDR1 transcripts were preserved in both SCZ and BD (Fig. 3A, B), so we used the whole dataset to evaluate the association of each module with SCZ and BD [38]. Twenty-nine modules were detected in the whole dataset. Eight DDR1 transcripts were scattered across three modules (Mws4, Mws13 and Mws32), and seven DDR1 transcripts did not show any pattern of coexpression with other genes and were assigned to Mws0. The MM of DDR1 transcripts ranged from 0.11 to 0.68 and is detailed in Supplementary Table 5. Mws4 contained ENST00000376575 (DDR1d) and ENST00000452441 (DDR1b) and was associated with SCZ and BD. Mws13 contained ENST00000460944 and was associated with SCZ. Mws32 contained ENST00000376567 (DDR1a), ENST00000376570 (DDR1a), ENST00000324771 (DDR1b), ENST00000428153 and ENST00000446312 and was associated with SCZ and BD (Fig. 3C and Supplementary Table 5). Of note, the MEs of Mws4, Mws13 and Mws32 were correlated with the cell type proportion estimates of the normalized whole sample matrix (Supplementary Table 6), which indicates that the variance explained by the cell type composition of the samples was not completely removed when building the network.
DDR1 transcript differential expression analysis and transcript significance in SCZ and BD
We observed that some DDR1 transcripts present in Mws4, Mws13 and Mws32 were differentially expressed in SCZ or BD (Fig. 4). ENST00000376575 (DDR1d) was downregulated in SCZ (log2FC = −0.21; adjP = 0.031), ENST00000460944 was downregulated in SCZ (log2FC = −0.25; adjP = 0.0012) and BD (log2FC = −0.28; adjP = 0.028), ENST00000376567 (DDR1a) was downregulated in SCZ (log2FC = −0.23; adjP=0.048), ENST00000376570 (DDR1a) was downregulated in SCZ (log2FC = −0.32; adjP=0.037) and BD (log2FC = −1.23; adjP = 3.2E-08), ENST00000418800 (DDR1a) was downregulated in BD (log2FC = −0.37; adjP = 0.015) and ENST00000513240 (DDR1c) was upregulated in SCZ (log2FC = 0.34; adjP = 0.0062). These associations were further validated by the transcript significance test, which showed that all transcripts differentially expressed in SCZ or BD were significantly correlated in the same direction with the same diagnosis (Supplementary Table 5). Although ENST00000376567 (DDR1a), ENST00000376575 (DDR1d) and ENST00000513240 (DDR1c) did not reach statistical significance in the differential expression analysis for BD after adjusting for multiple testing, they followed the same trend as in SCZ (Fig. 4), and they were significantly correlated with BD in the transcript significance test (Supplementary Table 5).
Discussion
Here, we studied DDR1 transcript coexpression in the adult human brain using transcriptomic data for the first time. Using expression data from HCs, we observed that coexpression modules including DDR1 transcripts were enriched in marker genes for astrocytes, OPCs, oligodendrocytes and neurons. Using expression data from patients with SCZ, patients with BD and HCs, we observed that all coexpression modules containing DDR1 transcripts were associated with SCZ or BD and that six DDR1 transcripts were differentially expressed in SCZ or BD (Fig. 5).
DDR1 transcripts in MHC1 (ENST00000376567 (DDR1a) and ENST00000452441 (DDR1b)) and MHC2 (ENST00000376570 (DDR1a), ENST00000324771 (DDR1b), ENST00000460944, ENST00000428153 and ENST00000484556) were coexpressed with genes involved in processes linked to the cell cycle, DNA repair and RNA processing, as revealed by GO enrichment analysis of the modules, which suggests that they may be expressed during cell division and proliferation. Cell enrichment analysis of the modules and correlation of DDR1 transcripts with cell type proportion estimates suggested that the expression of DDR1 transcripts in MHC1 is not restricted to a single cell type and that they are probably expressed in astrocytes and OPCs. Conversely, DDR1 transcripts in MHC2 are hub transcripts of the module and therefore could be specifically expressed in OPCs. An exception in MHC2 is ENST00000484556, which does not represent a hub transcript, forms a different cluster in the network visualization analysis and correlates mostly with neuron proportion estimates. Regarding the possible role of DDR1 transcripts in cell division and proliferation, previous research showed that DDR1 is recognized and upregulated by the p53 protein [17, 39]. p53 activates the checkpoint needed to avoid DNA damage accumulation during the cell cycle G1/S phase transition [40] and mediates cell prosurvival effects [39]. Accordingly, MHC1 and MHC2 are enriched in mitotic and/or cell cycle G1/S phase transition genes and include genes that induce the p53 cascade, such as TP53INP1, TP53BP1, TP53I3, TP53RK and TP53I11. Moreover, DDR1 has been reported to be involved in actin cytoskeleton remodeling [41], and DDR1 transcripts in MHC1 and MHC2 were coexpressed with genes involved in cell morphology and cytoskeleton organization in the network visualization analysis. It is thus possible that p53 upregulates these DDR1 transcripts during the cell cycle to ensure the maintenance of cell morphology in different cell types.
DDR1 transcripts in MHC3 (ENST00000508472) and MHC4 (ENST00000376568 (DDR1b)) could be expressed from late stages of OPC differentiation to early stages of oligodendrocyte differentiation/maturation, since they are hub transcripts of MHC3 and MHC4, which are enriched in OPCs and oligodendrocytes. Additionally, ENST00000376568 (DDR1b) correlates mostly with the OPC and oligodendrocyte proportion estimates. ENST00000508472 could be involved in the establishment of cell polarity during OPC migration, given the enrichment in the establishment or maintenance of cell polarity of MHC3, the coexpression with genes devoted to cell morphology and cytoskeleton organization in the network visualization analysis and the fact that ENST00000508472 is expressed at low levels, consistent with the low rate of OPC migration in adulthood [42]. ENST00000376568 (DDR1b) is coexpressed with genes upregulated in the final stage of newly formed oligodendrocytes and early stages of mature oligodendrocytes [43] and was previously observed to be locally translated in oligodendrocyte processes to synthesize myelin (MBP, MOBP, BCAS1 and PLEKHB1 [44, 45]). We previously demonstrated that DDR1 has an A2 response element (A2RE) sequence that is recognized by heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1, and silencing the hnRNP A2/B1 gene in an oligodendroglial cell line resulted in a downregulation of DDR1c concomitant with an upregulation of DDR1b [20]. Since ribonucleoproteins are involved in RNA‒protein packaging and transport from the nucleus to the cytoplasm periphery, we can speculate that ENST00000376568 (DDR1b) is the main isoform that is involved in myelination. Accordingly, previous studies have shown that DDR1 parallels the dynamics of myelination [9], is important for the compactness of the myelin sheath [46] and regulates the ensheathment, survival and caliber of peripheral axons [47]. Furthermore, DDR1 stabilizes cadherins in cell membranes [48, 49]. Here, module MHC4 contains the cadherin CDH19, a marker of myelin-forming cells [50]. Altogether, our results suggest that ENST00000376568 (DDR1b) can stabilize cadherin contacts between oligodendrocyte and neuron membranes or, alternatively, between concentric oligodendrocyte membranes required for compacting the myelin sheath, as previously suggested [22, 46]. In addition, the expression of ENST00000376568 (DDR1b) in oligodendrocytes is supported by single-cell transcriptome studies showing that DDR1 is mainly expressed in oligodendrocytes in the human brain (www.proteinatlas.org).
The DDR1 transcript of MHC5 (ENST00000376569 (DDR1a)) is a hub transcript that may be expressed in interneurons and contribute to synapse stabilization, since it is highly enriched in neurons and processes related to synapses and contains most of the classical interneuron genes, namely, SST, CCK, GAD1, PVALB, CALB1, VIP, LAMP5, RELN and NOS1 [51]. Accordingly, the expression of DDR1 is higher in inhibitory than in excitatory neurons (www.proteinatlas.com). Recent research reported the expression of DDR1 in mature GABA neurons of the mouse adult brain [52], and some studies aimed at the characterization of the expression profile of the different types of interneurons have detected a slight expression of DDR1 across all types of interneurons, especially in those expressing SST or CCK [53, 54]. Interneurons are enfolded by perineuronal nets, a specialization of the extracellular matrix required for controlling the plasticity of the central nervous system [55]. Interestingly, DDR1 binds some types of collagens, important constituents of the extracellular matrix [56], and collagen XIX has been demonstrated to be pivotal for the stability of perineuronal nets and synapse formation [57, 58]. To the best of our knowledge, the binding of DDR1 to collagen XIX has not yet been studied, but the collagen XIX gene (COL19A1) is located in MHC5. Altogether, we hypothesize that ENST00000376569 (DDR1a) may bind to some components of the perineuronal net, leading to the stabilization of newly formed synapses.
DDR1 transcripts of MHC0 could not be assigned a function by enrichment analyses. Among them, we observed that the expression of ENST00000418800 (DDR1a), ENST00000508312 (DDR1e) and ENST00000446312 correlated mostly and negatively with the cell proportion estimates of neurons and that ENST00000513240 (DDR1c) and ENST00000508312 (DDR1e) correlated mostly with the cell proportion estimates of endothelial cells. However, the correlation coefficients are low ( < 0.4), which precludes the assignment of these transcripts to a specific cell type. According to the present results, we previously reported that the average methylation level of DDR1 in isolated neuronal nuclei correlated negatively with the average methylation level of neuron markers such as MAP2 and MAPT [59]. In addition, in a previous study, we detected the expression of DDR1 in brain tissue endothelial cells by means of in situ hybridization and immunohistochemistry. Specifically, we detected signals using antibodies against the DDR1 extracellular domain but not with antibodies against the intracellular domain [12]. This observation is consistent with the fact that ENST00000376575 (DDR1d), which lacks the intracellular domain, correlates with the cell proportion estimates of endothelial cells.
The expression of DDR1 in activated microglia has been reported in some studies [60, 61]. Using coexpression modules in different stages of human brain development, we observed that the expression of DDR1 in microglia was relevant in the prenatal period, the first years of life ( < 6 years) and late adulthood ( ≥ 40 years), but not the period between 7 and 40 years of age [23]. Here, we found that some transcripts correlated with the cell proportion estimates of microglia, especially ENST00000376570 (DDR1a), which showed a negative correlation, in agreement with our previous observation that the average methylation level of DDR1 in brain tissue correlated negatively with the average methylation level of the microglia marker CX3CL1 [59]. However, in the present study the correlation coefficient was not sufficiently high ( < 0.4) to draw conclusions about the relevance of this transcript in microglial function.
Regarding the involvement of DDR1 in psychiatric disorders, our results indicate that DDR1 transcript expression is more altered in SCZ than in BD. All modules containing DDR1 transcripts in the whole sample network (Mws4, Mws13 and Mws32) were associated with SCZ, while only Mws32 was associated with BD. However, it is worth noticing that the MEs of Mws4, Mws13 and Mws32 were correlated with cell-type proportion estimates of many cell types, which could partly explain the associations of Mws4, Mws13 and Mws32 with the diagnoses of SCZ and BD. On the other hand, he expression of 6 transcripts was altered in the differential expression and transcript significance analyses in SCZ, while that of only 3 transcripts was altered in the transcript significance test in BD (Fig. 5). According to our interpretation, some transcripts downregulated in psychiatric disorders (ENST00000376567 (DDR1a), ENST00000376570 (DDR1a), ENST00000460944) may be involved in the maintenance of the cell morphology of astrocytes and OPCs during the cell cycle. The only transcript that was upregulated in disease was ENST00000513240 (DDR1c), in agreement with previously published evidence that DDR1c is upregulated in patients with SCZ [21]. In addition, the transcript ENST00000418800 (DDR1a) was downregulated only in BD. Whether alterations in the expression of DDR1 transcripts are a cause or a consequence of the disease cannot be inferred from our results. However, according to previous evidence, the upregulation of ENST00000513240 (DDR1c) may be at least partly attributable to genetic variation, since minor alleles of rs2267641 and rs1264323 are associated with SCZ diagnostic and cognition speed processing [4, 62] and with higher levels of ENST00000513240 (DDR1c) in SCZ [22]. Overall, our study suggests that patients with SCZ and BD present a downregulation of DDR1a during the cell cycle of astrocytes and OPCs and proposes that the expression of other DDR1 transcripts is also altered in other cell types. This evidence supports the growing awareness that the cell type-specific regulation system should be considered in order to disentangle the complex physiopathology of SCZ and BD [63, 64], which could help the development of more specific therapies based upon the targeting of specific cells, a promising strategy currently in development in other fields [65]. We hope that future research using single-cell transcriptomic data will validate the results presented here.
Limitations
The present study has some limitations that are worth mentioning. First, our results do not represent the direct measurement of mRNA in single cells but rather the indirect inference of single-cell mRNA expression based on the principle of “guilt by association” [27]. Second, a shared pattern of expression does not necessarily mean physical proximity, and we did not perform in situ experiments to confirm the pattern of DDR1 isoform expression across cell types. Third, our analyses only considered the six major types of brain cells, while there is a growing trend toward cell subtype classification of brain cells [43, 52, 66]. Fourth, our results are based on samples from the dorsolateral prefrontal cortex, and different results may be found in other brain regions [67]. Fifth, many DDR1 transcripts were filtered out at the beginning of the analysis, and thus, the landscape of DDR1 expression and function was not fully addressed. Sixth, the results of the differential expression analysis should be replicated in independent samples.
Data availability
Data used in this manuscript was retrieved from PsychENCODE and no new data was generated.
Code availability
Code is available upon request from the corresponding author.
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Acknowledgements
The study was supported by grants from the Brain and Behavior Research Foundation (2017 NARSAD Independent grant, #25811 to EV), Instituto de Salud Carlos III (Research Project PI18/00954 to EV), and Agència de Gestió d’Ajuts Universitaris i de Recerca (2021SGR01065 to EV). Selena Aranda was the recipient of a predoctoral fellowship granted by the Instituto de Salud Carlos III (PFIS FI19/00268). Data were generated as part of the PsychENCODE Consortium, supported by: U01DA048279, U01MH103339, U01MH103340, U01MH103346, U01MH103365, U01MH103392, U01MH116438, U01MH116441, U01MH116442, U01MH116488, U01MH116489, U01MH116492, U01MH122590, U01MH122591, U01MH122592, U01MH122849, U01MH122678, U01MH122681, U01MH116487, U01MH122509, R01MH094714, R01MH105472, R01MH105898, R01MH109677, R01MH109715, R01MH110905, R01MH110920, R01MH110921, R01MH110926, R01MH110927, R01MH110928, R01MH111721, R01MH117291, R01MH117292, R01MH117293, R21MH102791, R21MH103877, R21MH105853, R21MH105881, R21MH109956, R56MH114899, R56MH114901, R56MH114911, R01MH125516, and P50MH106934 awarded to: Alexej Abyzov, Nadav Ahituv, Schahram Akbarian, Alexander Arguello, Lora Bingaman, Kristin Brennand, Andrew Chess, Gregory Cooper, Gregory Crawford, Stella Dracheva, Peggy Farnham, Mark Gerstein, Daniel Geschwind, Fernando Goes, Vahram Haroutunian, Thomas M. Hyde, Andrew Jaffe, Peng Jin, Manolis Kellis, Joel Kleinman, James A. Knowles, Arnold Kriegstein, Chunyu Liu, Keri Martinowich, Eran Mukamel, Richard Myers, Charles Nemeroff, Mette Peters, Dalila Pinto, Katherine Pollard, Kerry Ressler, Panos Roussos, Stephan Sanders, Nenad Sestan, Pamela Sklar, Nick Sokol, Matthew State, Jason Stein, Patrick Sullivan, Flora Vaccarino, Stephen Warren, Daniel Weinberger, Sherman Weissman, Zhiping Weng, Kevin White, A. Jeremy Willsey, Hyejung Won, and Peter Zandi. The Genotype-Tissue Expression (GTEx) Project was supported by the Common Fund of the Office of the Director of the National Institutes of Health and by NCI, NHGRI, NHLBI, NIDA, NIMH, and NINDS. The data used for the analyses described in this manuscript were obtained from the GTEx Portal on 05/05/2022.
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SA carried out the analyses with the guidance of GM. EV and GM designed the study. SA prepared the first draft of the manuscript and all authors critically revised and approved the final version.
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Aranda, S., Muntané, G. & Vilella, E. Coexpression network analysis of the adult brain sheds light on the pathogenic mechanism of DDR1 in schizophrenia and bipolar disorder. Transl Psychiatry 14, 112 (2024). https://doi.org/10.1038/s41398-024-02823-0
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DOI: https://doi.org/10.1038/s41398-024-02823-0
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