Abstract
Synaptic abnormalities have been described in individuals with autism spectrum disorders (ASD). The cell-adhesion molecule Neuroligin-3 (Nlgn3) has an essential role in the function and maturation of synapses and NLGN3 ASD-associated mutations disrupt hippocampal and cortical function. Here we show that Wnt/β-catenin signaling increases Nlgn3 mRNA and protein levels in HT22 mouse hippocampal cells and primary cultures of rat hippocampal neurons. We characterized the activity of mouse and rat Nlgn3 promoter constructs containing conserved putative T-cell factor/lymphoid enhancing factor (TCF/LEF)-binding elements (TBE) and found that their activity is significantly augmented in Wnt/β-catenin cell reporter assays. Chromatin immunoprecipitation (ChIP) assays and site-directed mutagenesis experiments revealed that endogenous β-catenin binds to novel TBE consensus sequences in the Nlgn3 promoter. Moreover, activation of the signaling cascade increased Nlgn3 clustering and co- localization with the scaffold PSD-95 protein in dendritic processes of primary neurons. Our results directly link Wnt/β-catenin signaling to the transcription of the Nlgn3 gene and support a functional role for the signaling pathway in the dysregulation of excitatory/inhibitory neuronal activity, as is observed in animal models of ASD.
Similar content being viewed by others
Introduction
Autism spectrum disorders (ASD) are highly heterogeneous, pervasive neurodevelopmental disorders characterized by persistent impairments in reciprocal social communication and repetitive patterns of behaviors.1 Although its etiology is unknown, ASD prevalence appears to be increasing.2,3,4,5 Family and twin studies have established ASD as highly inheritable diseases with a 90% phenotypic concordance among monozygotic twins.6 ASD mutations range from either common or de novo single nucleotide to copy number variants, to large-scale DNA deletions, duplications or translocations.7,8 According to SFARI Gene9 there are more than 800 genes implicated in ASD, with at least 50 high-ranking candidate risk genes, including the Neuroligin-3 gene (NLGN3: Gene ID: 54413, ChrXq13.1), which is strongly enriched with variants that affect ASD risk.10,11,12,13
Neuroligins (Nlgns) are postsynaptic cell-adhesion molecules that act as ligands for presynaptic Neurexins (Nrxns).14,15,16 Five different NLGN genes have been described in humans (NLGN1-3/4X/4Y), and since the first description of NLGN3 and NLGN4 as candidate genes for ASD10 all other NLGN genes have been associated with the disorder.17,18,19 Moreover, genes coding for Nlgn-interacting partners in the postsynaptic density, such as some NRXN20,21,22 and SHANK family members, have also been implicated with ASD,23,24,25 supporting an essential role for Nlgn complexes in the onset or development of ASD. At the functional level, Nlgns enhance the formation and maturation of specific types of synapses. For instance, while Nlgn1 is found preferentially in excitatory and Nlgn2 in inhibitory and cholinergic synapses,26,27 Nlgn3 localizes at both excitatory and inhibitory synapses.28,29,30,31,32 Nlgn4X has been observed in inhibitory glycinergic synapses.33 Currently, mice expressing Nlgn3 mutated forms display autism-like behaviors and are highly sought as experimental models for the study of ASD pathology.34,35
We predicted earlier that sustained gain-of-function of Wnt/β-catenin signaling in the developing brain could be responsible for the onset/development of ASD and that this effect involves the additive effect of genetic variants within components and/or genes whose products modulate its functional activity.36 This hypothesis has received considerable attention recently,37,38,39,40 mainly since it has been observed that 39% of the more disruptive de novo mutations in ASD family trios were found within interconnected networks containing chromatin remodeling, synaptic, and Wnt/β-catenin signaling genes.41,42 Likewise, integration of RNA-seq expression profiles during brain development with protein–protein interaction networks have identified highly modules enriched of connected Wnt signaling genes associated with ASD.43 At the genetic level, several Wnt/β-catenin components have been associated with ASD, including the canonical Wnt2 ligand,44 Wnt/β-catenin target genes Engrailed 2 (EN2)45 and the hepatocyte growth factor receptor (MET),46,47,48 and cadherins encoding genes such as CDH5, CDH8, CDH9, CDH10, CDH13, CDH15, PCDH10, PCDH19, and PCDHb4,49 some of which interact with β-catenin in cell-cell adhesion complexes. More recently, the chromo-helicase domain protein 8 (CHD8),41,50,51 which inhibits β-catenin through direct binding,52 and DYRK1A that modulates Wnt/β-catenin signaling through interaction with the p120 catenin,53 have been found to be associated with ASD. Interestingly, CHD8 and DYRK1A harbor recurrent disruptive mutations and are highly correlated with head size abnormalities,51 a feature commonly observed in ASD. Moreover, rare de novo genetic variants in the β-catenin (CTNNB1) gene itself have been implicated in severe intellectual disability.54
Further support for a role of Wnt/β-catenin signaling in ASD comes from pharmacological studies, research in ASD comorbidities such as Tuberous sclerosis or in the recognition that an abnormal immune response plays an important role in the onset or development of the disorder.55,56,57 First, in utero exposure to teratogens such as valproic acid (valproate; VPA) causes a higher incidence of ASD in the offspring58,59 and VPA is known to increase cytosolic and nuclear β-catenin levels and activate Wnt/β-catenin-dependent gene expression, by a complex mechanism involving inhibition of histone deacetylase (HDAC) and glycogen synthase kinase-3α/β (GSK3α/β) activities.60,61 Second, in Tuberous sclerosis the tumor suppressor complex formed by hamartin (TSC1 in Chr 9) and tuberin (TSC2 in Chr 16) interact with the β-catenin degradation complex and thus modulates the action of Wnt/β-catenin signaling.62,63 Finally, several inflammatory cytokines, including interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1) and interferon γ (IFN-γ), are elevated in peripheral blood cells, serum, plasma, cerebrospinal fluid, or in the brains of ASD children.64,65,66,67,68,69,70,71,72 In this context, recent evidence indicates that Wnt/β-catenin and non-canonical Wnt signaling have both pro- and anti-inflammatory activity73 and that the signaling cascade is involved in inflammation-driven brain damage and inflammation-directed brain repair.74 Wnt3a selectively increases the expression of proinflammatory immune response genes in microglia and enhances the release of de novo IL-6, IL-12, and TNF-α.75 Nevertheless, the transcriptional program elicited by Wnt/β-catenin signaling in different types of brain cells has received little attention.76,77,78,79
Therefore, considering that Wnt/β-catenin signaling has an essential role in ASD affected regions such as the frontal cortex and the hippocampal formation,80,81 that others and we have observed that Wnt/β-catenin signaling enhances excitatory neurotransmission in hippocampal neurons,82,83,84,85 and that the transcriptional program controlling Nlgn3 expression and function is currently unknown, here we investigated the functional effects of the signaling cascade on Nlgn3 expression and synaptic function in hippocampal neurons.
Materials and methods
Primary cultures of rat hippocampal neurons
Primary hippocampal neurons were obtained from E18 Sprague-Dawley rat embryos (randomly selected; male/female in equal proportion) as described.84,86 Cells were maintained for 14 days in vitro (DIV) on culture plates (3.5 × 105 cells/plate) coated with poly-l-lysine (Sigma-Aldrich, St. Louis, MO, USA). Primary neurons were grown on Neurobasal medium supplemented with B27 (Gibco BRL, Thermo Fisher Scientific, Waltham, MA, USA) and 50% media was replaced every 3 days. Cells were kept at 37 °C in 5% CO2 incubator and saturated humidity. The study protocol with rat embryos was approved by the Bioethical Committee of Universidad Andres Bello, Chile (026/2013) and was conducted in accordance with the Ethical Guidelines for Treatment of Laboratory Animals of the National Commission on Science and Technology (CONICYT- Chile).
Cell lines
HT22 mouse hippocampal cells87,88,89,90 (a gift from Dr. Randall T. Moon, University of Washington, WA, USA), HEK293T, Wnt3a-L-cells mouse fibroblasts and control-L-cells (CRL-3216, CRL-2647, and CRL-2648, respectively; ATTC, Rockville, MD, USA), were maintained in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin (Gibco BRL, Thermo Fisher Scientific), 1% P/S (Invitrogen, Thermo Fisher Scientific) and kept at 37 °C in 5% CO2 incubator with saturated humidity.
Wnt3a purification
Wnt3a is a specific Wnt/β-catenin signaling agonist that can be efficiently recovered from conditioned medium from Wnt3a-secreting L-cells (Wnt3a-CM). Wnt3a purification was carried out, as previously described.84,91,92 The presence of the Wnt3a protein was detected with an anti- Wnt3a antibody (R & D Systems, Minneapolis, MN, USA). Purity was analyzed by SDS–PAGE (8%), stained with Coomassie Blue G250, and analyzed through densitometry by using software ImageJ.93
Semi- and quantitative-PCR (q-PCR) analysis
Primary cultures of hippocampal neurons or HT22 cells were seeded in 6 well culture plates (5 × 105 cells per ml) and stimulated with 200–400 ng/ml of purified Wnt3a protein or with 10–20 mM LiCl (Sigma-Aldrich) and collected for mRNA or protein analyses, as described.94,95
Briefly, total RNA was extracted in RNase free conditions using TRIzol reagent (Thermo Fisher Scientific) and 2 µg of RNA was reverse transcribed with Affinity Script QPCR cDNA synthesis kit (Agilent Technologies, Santa Clara, CA, USA). q-PCR was performed in a Stratagene Mx3005P thermal cycler using 40 ng of cDNA, Brilliant II SYBR Green qPCR Master Mix (Agilent Technologies), and 200–400 nM of primers targeting known Nlgn3 mouse and rat mRNA isoforms (Supplementary Table S1). Thermal cycling conditions included an initial activation step at 95 °C for 10 min and 40 cycles of denaturing at 95 °C, annealing at 60 °C, and amplification at 72 °C for 15 s. Amplification was checked for a single product by analyzing the melting curve, and the sizes of each product were confirmed by gel electrophoresis using GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA). The expression levels of Nlgn3 and cMyc were normalized to Rpl13a expression, using the delta–delta Cq method (2−ΔΔCq), as described.96
shRNA experiments
Lentiviral constructs expressing shRNAs against mouse β-catenin (β-catenin-pLKO.1, #SHCLND-NM_007614) or control non-targeting shRNA (control-pLKO.1, #SHC002) were obtained from MISSION® (MISSION shRNA Library, Sigma-Aldrich). Lentiviral particles were produced in HEK293T cells co-transfected with the pLKO.1 construct, pCMV-dR8.91 (Delta 8.9) plasmid (containing gag, pol and rev genes) and pVSV.G plasmid (at a 3:2:1 ratio). Transfections were performed using Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific). Lentiviral particles were harvested 48 h post transfection, filtered through a cellulose acetate filter (0.45 µm) and concentrated by centrifugation (3,800 rpm at 4 °C for 30 min) with a 100 kDa Ultra15 Amicon filter (EMD Millipore, Billerica, MA, USA). Infections were carried out in HT22 cells plated in 60 mm diameter with 80–90 % confluence for 48 h using 100 µl concentrated virus.
Western blot
Nlgn3, β-catenin and β-actin proteins were analyzed by western blot using the H-55 anti-Nlgn3 antibody (SC-50395; 1:1000; Santa Cruz Biotechnology, TX, USA), the monoclonal E-5 β- catenin antibody (SC-7963; 1:2000; Santa Cruz Biotechnology) or with the anti-β-actin antibodies (SC-47778; 1:5000; Santa Cruz Biotechnology). Membranes were washed (3×) and incubated with appropriate secondary antibodies conjugated to horseradish peroxidase for 1 h at room temperature, washed and incubated for 2 min with enhanced chemiluminescence solution (Pierce ECL, Thermo Scientific, IL, USA) and exposed for 1–3 min on Carestream Kodak BioMax films (Sigma-Aldrich). Secondary antibodies were: goat-anti rabbit IgG-HRP (SC-2004 for Nlgn3, 1:5000; Santa Cruz Biotechnology), goat-anti mouse IgG-HRP (SC-2055, for β- catenin and β-actin; 1:5000; Santa Cruz Biotechnology).
Transcriptional activity of Nlgn3 promoter constructs
Nlgn3 promoter-luciferase fragments were generated by conventional PCR from genomic DNA extracted from either mouse HT22 hippocampal cells or Sprague-Dawley primary neurons using specific primers containing restriction sites (Supplementary Table S1) and subsequently inserted into the pGL3-Basic vector (Promega, Madison, WI, USA), as described.94,95 Activity of Nlgn3 promoters was measured in 80–90% confluent HEK293 cells seeded in 24 well culture plates. Cells were co-transfected using Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific) for 24 h with either pNL3 or pSUPERTOPFlash (STF) luciferase constructs,97 in the absence or presence of constitutively active β-catenin S33Y or the dominant negative ΔTCF4 constructs.98
The pRL-TK Renilla luciferase plasmid (1 ng) was used as an internal control. Firefly and Renilla luciferase activities were determined using the Dual-Luciferase Reporter Assay (Promega) in a Victor-3 multiplate reader (Perkin Elmer, Waltham, MA, USA). Promoter activity was normalized as the ratio between Firefly and Renilla luciferase units. Site-directed mutagenesis in TBE Sites II and III were generated using the pNL3Mm1.4 construct as background with M1 or M2 primers (Supplementary Table S1) with the QuickChange Site- Directed Mutagenesis kit (Stratagene, Agilent Technologies). All constructs were verified through direct sequencing (ABI-3130 Genetic Analyzer, Applied Biosystems, Foster City, CA, USA).
Chromatin immunoprecipitation (ChIP) assays
ChIP studies were performed in HT22 neurons, as described.94 Briefly, 2.0 × 107 cells were incubated with cell-conditioned medium containing Wnt3a (Wnt3a-CM; Wnt3a-CM 50% plus 50% of fresh HT22 incubation media), LiCl or CHIR 98014 (Sigma-Aldrich), as Wnt/β-catenin pharmacological agonists.99,100 Cells were cross-linked with 1% formaldehyde (Sigma-Aldrich) for 10 min at room temperature and then the reaction was stopped with 0.125 M glycine. Samples were sonicated with the M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA) using milliTube caps (Covaris), following the high cell chromatin shearing protocol suggested by the manufacturer. In total 25 µg of chromatin was used in each ChIP assay. Endogenous β-catenin bound to TBE Sites within the mouse Nlgn3 promoter was immunoprecipitated with rabbit anti β-catenin antibody (SC-7199; 4 µg; Santa Cruz Biotechnology). Cross-linked chromatin fragments averaging 200–300 bp were assessed by q-PCR as described above. Antibody specificity was assayed with normal rabbit-IgG (12–370; 4 µg; EMD Millipore).
Immunofluorescence and image processing
Control and Wnt3a-treated rat hippocampal neurons (14 DIV) cultured in poly-l-lysine (Sigma- Aldrich) coated coverslips (5.0 × 105 cells/plate) were fixed using 4% formaldehyde-4% sucrose (pH: 7.0) solution for 20 min at room temperature. Cells were permeabilized with Triton X-100 0.2 % for 5 min and treated with blocking solution (PBS 1 × /BSA 2%, pH: 7,4) for 30 min. Primary antibodies against Nlgn3 (H-55; SC-50395; 1:100), PSD-95 (N-18; SC-6926; 1:100), and MAP2 (H-300; SC-20172; 1:100) (Santa Cruz Biotechnology, USA) were used and Alexa Fluor Antibodies (1:400; Molecular Probes, Eugene, OR, USA) for secondary staining. Samples were examined on a LSM780 confocal microscopy (Carl Zeiss, Jena, Germany). Images were deconvolved using the ImageJ/FIJI101 plugin DeconvolutionLab102 using theoretical point spread functions. Total Nlgn3 fluorescence intensity was measured as arbitrary units in all dendritic processes with clear signal of MAP2 in 15 µm segments that consistently covered an area between 40 and 200 µm2. To illustrate colocalization between Nlgn3 and PSD-95 proteins, three-dimensional isosurfaces of dendritic processes were created using Imaris v8.2 (Bitplane, Concord, MA, USA) and by applying the ImarisSurface tool (smoothness, 0.2 µm; quality level, 5) on the MAP2 fluorescent signal. Nlgn3 and PSD-95 clusters inside of dendritic processes were rendered using the ImarisSpots tool. Co-localization coefficients of Nlgn3 and PSD-95 (Pearson and Manders) were determined using the ImarisColoc tool in an average of four dendrites per neuron, which were selected based on brightness103 and length (~15 µm). Briefly, while Pearson’s correlation coefficient is an adimensional parameter that establishes whether colocalization exists independent of signal strength, thus avoiding the possibility of false positives, the Manders coefficient represents directly the percentage of colocalization between two signals.
Statistical analysis
Data are presented as mean ± s.e.m. Each experiment was repeated three times with three replicates. All data processing and analysis were completed before unblinding of the analyzer. Sample size was chosen according to previous reports and our pre-experiments. A minimum number of three biological replicates were performed to ensure reproducible and robust changes. No cell samples or animals were excluded from the analysis. Data were analyzed with Prism v5 (GraphPad Software, La Jolla, CA, USA). For the comparison of more than two groups, we used one-way ANOVA test followed by Kruskal–Wallis post hoc test. To compare two groups, we performed a two-tailed Mann–Whitney test. Statistically significant p-values are shown as *P < 0.05, **P < 0.01 and ***P < 0.001.
Results
Enhancement of Nlgn3 expression via Wnt/β-catenin signaling in hippocampal cells
According to genome wide data on β-catenin and TCF/LEF chromatin occupancy,78,104,105,106 most Wnt/β-catenin target genes have regulatory sequences within 2.5 kb clustering around transcriptional start sites (TSS). We searched for core TCF/LEF-binding elements (TBE: CTTTG) known to mediate β-catenin transcriptional activation of Wnt target genes, including a 3000 bp region upstream of the predicted transcriptional start site (TSS) in human, mouse and rat Nlgn3 gene sequences (Fig. 1a). We found multiple TBE sites in evolutionary conserved regions (ECR browser),107 suggesting a conserved role for the signaling cascade as a transcriptional regulator of Nlgn3 genes.
a Top: Genomic context of human NLGN3 in the long arm of chromosome X and schematic exon-intron boundaries of the gene. White and gray boxes: 5′ and 3′ UTR and exons, respectively. Middle: Conservation profile of the human NLGN3 promoter sequence compared with similar genomic regions in Mus musculus and Rattus norvegicus (50–100%). Bottom: Schematic representation of potential TCF/LEF sites (TBE: CTTTG, circles) found in these species. ECR: Evolutionary conserved region. b Early expression levels of Nlgn3 and cMyc genes after 2 h treatment with increasing doses of either purified Wnt3a protein or LiCl in HT22 hippocampal cells or rat primary hippocampal neurons (RHN). Rpl13a was used as a reference gene. c Quantitative determination of Nlgn3 and cMyc mRNA levels after 2 h treatment with Wnt3a (200–400 ng/ml) protein or LiCl (10–20 mM) in HT22 hippocampal cells and RHNs. d TOP: protein levels of β-catenin after 48 h treatment with β-catenin-shRNA. shRNA of GFP was used as control and β-actin as a loading control. Bottom: expression levels of Nlgn3 and β- catenin. e Nlgn3 and β-catenin protein levels in HT22 cells and RHNs after 6 h treatment with 200 and 400 ng/ml of purified Wnt3a protein. β-actin was used as a loading control. In (c) and (d), data represent mean ± s.e.m., *P < 0.05, **P < 0.01, two-tailed Mann–Whitney test
We have previously shown that Wnt/β-catenin target gene expression is rapidly induced (2–4 h) upon activation of the signaling cascade in different cell lines and primary cultures of hematopoietic cells.94,95,108 We therefore stimulated mouse HT22 hippocampal cells for 2 h with increasing concentrations of purified Wnt3a protein (200 and 400 ng/ml) or with LiCl (10 and 20 mM), which acts as a pharmacological inducer of Wnt/β-catenin signaling.86,99 We observed that both treatments increased Nlgn3 expression (Fig. 1b) and that the effect was paralleled by induction of the known Wnt/β-catenin target gene cMyc.109 The increase in Nlgn3 expression was consistently replicated in 14 DIV primary cultures of hippocampal neurons following short- term Wnt3a or LiCl treatments. Quantitative determination of mRNA levels in cells similarly treated confirmed that there was a significant increase in Nlgn3 and cMyc expression in response to Wnt/β-catenin activation (>2.5 and >3-fold induction, respectively; *P < 0.05; n = 3) (Fig. 1c) and that the transcriptional effect was clearly observed after 4 h treatment. Conversely, infection of HT22 cells with shRNA against mouse β-catenin for 48 h significantly decreased endogenous Nlgn3 expression (up to 35%; Fig. 1d). In addition, augmented protein levels of β-catenin and Nlgn3 were readily observed in HT22 cells and hippocampal neurons after 6 h Wnt3a treatment (200 and 400 ng/ml; Fig. 1e), at a time when most β-catenin is found within the nucleus of these cells (Supplementary Figure S1). We concluded that Wnt/β-catenin signaling is involved in the transcriptional program that controls Nlgn3 expression.
Contribution of TCF/LEF binding elements in mammalian Nlgn3 promoter activity
To investigate the contribution of Wnt/β-catenin responsive TBE sites on the transcriptional activity of the mouse Nlgn3 promoter, we initially cloned a 2849 bp genomic segment upstream of the luciferase gene in the pGL3-basic vector (pNL3Mm2.8) and assayed its activity through transient transfections in HEK293 cells. This promoter region included 2448 bp upstream of the TSS (Nlgn3-001 transcript, ENSMUST00000065858 used as reference), 87 bp of the unstranslated exon 1 and 314 bp of intron 1, contained nine putative TBE sites (Fig. 2a) and displayed significant basal transcriptional activity (Fig. 2b), indicating that the pNL3Mm2.8 reporter maintains necessary elements to support Nlgn3 gene expression. As suspected, a significant enhancement in pNL3Mm2.8 reporter activity occurred when HEK293 cells were co-transfected for 24 h with increasing concentrations of a construct coding for a constitutively active β-catenin (S33Y)98 protein (ca. 5 fold promoter activity; P < 0.05, n = 3; Fig. 2c).
a Genomic context of mouse Nlgn3 promoter and different Nlgn3 chimeric promoter constructs (pNL3Mm). Circles indicate potential TBE consensus sequences in the predicted regulatory sequence of Nlgn3 gene. Distances are in reference to the transcription start site (TSS). b Transient transfection of increasing doses (30, 60, and 90 ng) of Nlgn3 chimeric promoter constructs pNL3Mm2.8, pNL3Mm1.4, and pNL3Mm0.3 in HEK293 cells for 24 h. c Transient co-transfection of pNL3Mm2.8 kb or pNL3Mm1.4 with increasing doses of constitutively active β-catenin (S33Y) for 24 h. d Effects of dominant negative delta-TCF4 (ΔTCF4) on β-catenin induced pNL3Mm1.4 promoter activity. In (c, d) SuperTOPFLASH (STF) activity was measured as a positive control for Wnt/β-catenin pathway activation. RLU: relative luciferase units. In (b, c) Data represent mean ± s.e.m., *P < 0.05, one-way ANOVA and Kruskal–Wallis post hoc test. In (d) Data represent mean ± s.e.m., **P < 0.01, two-tailed Mann–Whitney test. ns not significant
To identify the minimal promoter with maximal response to Wnt/β-catenin signaling, we serially-deleted potential TBE sites, using the pNL3Mm2.8 construct as background. We observed that the activity of pNL3Mm1.4 (1,362 bp, −961/+401), containing TBE sites I–IV and basal elements, was significantly augmented in the presence of constitutively active β-catenin (S33Y) co-transfected for 24 h (>3-fold; p < 0.05; Fig. 2c). In addition, we observed that a third 244 bp construct (pNL3Mm0.3) containing only intron 1 and TBE site I (+157/+401) did not support basal reporter transcription (Fig. 2b) and thus it was discarded for subsequent experiments. Next, the transcriptional activity pNL3Mm1.4 was explored in loss-of-function assays with a dominant-negative TCF4 (ΔTCF4) construct, which codes for a transcription factor lacking 30 residues from its amino-terminus and that is unable to bind β-catenin.98 We found that the enhancement induced by β-catenin on pNL3Mm1.4 promoter activity was significantly antagonized ( > 50%) by co-expressing ΔTCF4 for 24 h (Fig. 2d), corroborating that β-catenin- mediated transcriptional activation involves association with TCF/LEF family members. Finally, these gain and loss-of-function experiments were consistently replicated using four additional rat Nlgn3 promoter constructs (pNL3Rn2.8: 2098 bp, −1786/+312; pNL3Rn1.4: 1377 bp, −1065/+312, pNL3Rn1.1: 1116 bp, −1786/−671; and pNL3Rn0.6: 566 bp, −2098/−1221; Supplementary Figure S2), indicating that pNL3-1.4 represents an evolutionary conserved promoter region, which exhibits basal activity and a significant induction in response to Wnt/β-catenin modulation.
Novel and functional TBE site in the promoter of the Nlgn3 gene
The binding of endogenous β-catenin to conserved TBE sites on the Nlgn3 promoter was analyzed through chromatin immunoprecipitation (ChIP) assays in HT22 cells. We observed that a significant binding of β-catenin to TBE sites II and III within the mouse Nlgn3 promoter was induced after treatment with either cell-conditioned media containing Wnt3a (Wnt3a-CM, see Methods) for 4 h or 20 mM LiCl for 24 h (rapid or persistent response, respectively) (Fig. 2a). In addition, direct binding of β-catenin to TBE sites II–III was similarly observed in HT22 cells incubated for 4 h with CHIR 98014 (Fig. 2b), which acts as a specific inhibitor of GSK3β activity and thus enhances nuclear and cytosolic levels of β-catenin.100 Finally, since these experiments did not resolve whether β-catenin binds directly to Nlgn3 TBE Site II and/or TBE Site III (mainly due to their close spatial proximity), we introduced through site-directed mutagenesis two-nucleotide changes in each TBE sequence, using pNL3Mm1.4 as the template (Fig. 3c). Remarkably, transient transfection of these constructs in HEK293 cells for 24 h revealed that mutations affecting only the consensus sequence of TBE site III (pNL3Mm1.4-MIII) completely abrogated β-catenin activation of the pNL3Mm1.4 reporter construct. From these experiments, we conclude that TBE site III (−378/−382) is a functional Wnt/β-catenin transcriptional element within the Nlgn3 promoter.
a Top panel: Representation of the 1.4 kb mouse Nlng3 promoter construct depicting the five TBE sites analyzed by ChIP assays. Bottom panel: Endogenous β-catenin binding to TBE Sites in the mouse Nlgn3 promoter examined in HT22 cells after treatment with either Wnt3a-CM for 4 h (left) or 20 mM LiCl for 24 h (right). Data represent mean ± s.e.m., **P < 0.01, ***P < 0.001, two-tailed Mann–Whitney test. b TBE sites (II–VI) were similarly examined by ChIP assays in HT22 cells under control conditions or treated with 1 µM CHIR 98014 for 4 h. In (a and b) immunoglobulin G (IgG) was used as a control. c Top panel: Representation of changes introduced by site-directed mutagenesis in TBE Sites II and III in the context of the 1.4 kb mouse Nlng3 promoter construct. TBE consensus sequence: CTTTG; Mutated TBE sequence: CCTCG. Bottom panel: Transient transfection of increasing doses of mutant plasmids in the presence of constitutively active β-catenin (S33Y) in HEK293 cells for 24 h. RLU: relative luciferase units. Data represent mean ± s.e.m., *P < 0.05, one-way ANOVA and Kruskal–Wallis post hoc test. ns not significant
Wnt/β-catenin signaling enhances Nlgn3 clustering
Nlgn3 promotes synapse formation and localizes at excitatory or inhibitory synapses, where it interacts with postsynaptic proteins PSD-95 or gephyrin, respectively.29,30,32 Given that we reported that purified Wnt3a modulated intracellular calcium and enhanced excitatory neurotransmission in hippocampal neurons,84 we hypothesized that the signaling cascade may enhance synaptic function by increasing Nlgn3 levels in dendritic processes, where it would interact with PSD-95 in the postsynaptic terminal. Therefore, we incubated 14 DIV rat hippocampal neurons in the absence or presence of purified Wnt3a (400 ng/ml) for 2 or 24 h and performed immunofluorescence analyses to examine whether activation of the signaling cascade affects the differential recruitment of Nlgn3 to dendritic processes. First, Nlgn3 was distributed along dendrites in a punctate manner, in agreement to previous reports,32 where it regularly colocalized with PSD-95 protein (Fig. 4a). Notably, after 24 h Wnt3a incubation we observed that the average Nlgn3 intensity fluorescence was significantly increased in dendrites compared to control primary neurons (Fig. 4b). We were not able to detect differences in Nlgn3 fluorescence after short-term Wnt3a treatment (2 h). Second, we examined the effect of the Wnt3a ligand in Nlgn3 and PSD-95 clustering by measuring the intensity and surface area of individual clusters in dendritic processes. We observed that both Nlgn3 and PSD-95 cluster intensity and surface area increased after 24 h Wnt3a treatment (Supplementary Figure S3a), indicating that these excitatory synaptic proteins are differentially recruited to potential synaptic sites. We further measured the area covered by these proteins along dendrites and observed that Nlgn3 surface area, but not PSD-95 surface area, was significantly increased after 24 h Wnt3a treatment (Supplementary Figure S3b). Finally, three-dimensional volume rendering of Nlgn3 and PSD-95 clusters further revealed a significant overlap of Nlgn3 and PSD-95 signals (Fig. 4c), and confirmed that Wnt3a signaling induces Nlgn3 clustering and colocalization with PSD-95 protein, likely participating in excitatory postsynaptic assembly.
a Left panel: Representative confocal images of dendritic processes showing MAP2 (blue), Nlgn3 (red), and PSD-95 (green) immunofluorescence signals in primary hippocampal neurons under control conditions or treated with 400 ng/ml of purified Wnt3a protein for 2 h. White bar represents 2.5 µm. Right panel: Total Nlgn3 intensity fluorescence. b Same as in (a) but after 24 h Wnt3a treatment. c Left panel: Three-dimensional isosurface rendering of dendritic processes (smoothness, 0.2 µm; quality level, 5) and florescence intensity of Nlgn3 and PSD-95 clusters. Right panel: Colocalization coefficients for Nlgn3–PSD-95 interaction in control versus Wnt3a-treated neurons (Pearson and Manders, respectively). Each figure corresponds to three independents experiments (a ctrl n = 44 and Wnt3a n = 21; b ctrl n = 44 and Wnt3a n = 22; c ctrl n = 16 and Wnt3a n = 12). Data represent mean, *P < 0.05, **P < 0.01, two- tailed Mann–Whitney test. ns not significant
DISCUSSION
An excess of synapses in frontal, temporal and parietal lobes has been found in children with ASD compared to age-matched controls110,111 and changes in synaptic structure are detected in multiple mouse models of ASD.112 In this context, due to its participation in synaptic structure and maturation since early development, postsynaptic cell-adhesion molecules such as Nlgns and associated partners are major candidate genes to understand ASD. Nevertheless, very little is known regarding the transcriptional program that controls the expression of these molecules. Here we have shown that Wnt/β-catenin signaling induces a dose-dependent enhancement of Nlgn3 mRNA and protein levels, which is observed soon after induction of the signaling cascade and within the time frame observed for several other Wnt/β-catenin target genes whose promoters contain TBE sites.77,113 We detected that endogenous β-catenin was predominantly associated with the Nlgn3 promoter region spanning TBE site III (−378/−382) under control conditions, and that such interaction was clearly enhanced when cells were incubated in presence of signaling agonists. While our results represent the first functional characterization of the Nlgn3 promoter, we note that Nlgn1 and Nlgn2 have already been predicted by bioinformatics approaches as potential β-catenin target genes.78 Accordingly, activation of the signaling cascade by the chemical compound curcumin has been shown to effectively enhance Nlgn1 expression in endogenous neural stem cells.114 Interestingly, curcumin has shown anti- inflammatory and antioxidant properties and is being considered as a therapeutic option in cancer and various prevalent neurological disorders, including ASD.115,116,117
It is widely accepted that Wnt/β-catenin signaling is essential for brain development and function and is increasingly recognized that the cascade has a central role in ASD neurodevelopmental pathology. Indeed, Wnt/β-catenin signaling is involved in neurogenesis,118 axonal remodeling,119 patterning and maturation of functional synapses120,121,122 and excitatory neurotransmission.82,83,84,85 Likewise, increased β-catenin levels are critical for synaptic structure and dendritic arborization.123,124,125 In this regard, increased Nlgn3 intensity and Nlgn3 co-localization with PSD-95 after 24 h Wnt3a treatment supports the idea that the signaling cascade is involved in excitatory synaptogenesis through recruiting and clustering of proteins in dendritic processes. For instance, while large clusters of PSD-95 are indicative of spine stabilization126 it has been observed that similar treatment of hippocampal neurons with canonical Wnt7a, consistently increased PSD-95 puncta in a time-dependent manner and in a calcium/calmodulin-dependent protein kinase II (CAMKII) dependent mechanism.122 Interestingly, CAMKII activation is dependent of calcium entrance into the synaptic terminal and our group has already shown that Wnt3a increases transient calcium currents.84 Altogether, these data support a regulatory role for this kinase in ASD and other neurodevelopmental diseases.127,128
Cortical neuronal activity exerts mitogenic effects on neural and oligodendroglial precursor cells129 and recent experiments have shown that this neuronal activity involves the shedding of the Nlgn3-extracellular-domain from the postsynaptic surface.130 It would be interesting to examine whether Nlgn3 expression and clustering promoted by the Wnt/β-catenin signaling cascade leads to Nlgn3 shedding and how this process relates to neuroinflammation and defects in excitatory/inhibitory neurotransmission.131,132
References
Ozonoff, S. Editorial perspective: autism spectrum disorders in DSM‐5–An historical perspective and the need for change. J. Child. Psychol. Psychiatry 53, 1092–1094 (2012).
Kim, Y. S. et al. Prevalence of autism spectrum disorders in a total population sample. Am. J. Psychiatry 168, 904–912 (2011).
Baio, J. Prevalence of autism spectrum disorders: autism and developmental disabilities monitoring network, 14 sites, United States, 2008. Morbidity and mortality weekly report surveillance summaries. Cent. Dis. Control Prev. 61, 1–24 (2012).
Elsabbagh, M. et al. Global prevalence of autism and other pervasive developmental disorders. Autism Res 5, 160–179 (2012).
Home, C. Prevalence of autism spectrum disorder among children aged 8 years—autism and developmental disabilities monitoring network, 11 sites, United States, 2010. Morbidity and mortality weekly report surveillance summaries. Cent. Dis. Control Prev. 63, 1–24 (2014).
Rosenberg, R. E. et al. Characteristics and concordance of autism spectrum disorders among 277 twin pairs. Arch. Pediatr. Adolesc. Med. 163, 907–914 (2009).
Gaugler, T. et al. Most genetic risk for autism resides with common variation. Nat. Genet. 46, 881–885 (2014).
de la Torre-Ubieta, L., Won, H., Stein, J. L. & Geschwind, D. H. Advancing the understanding of autism disease mechanisms through genetics. Nat. Med. 22, 345–361 (2016).
Basu, S. N., Kollu, R. & Banerjee-Basu, S. AutDB: a gene reference resource for autism research. Nucleic Acids Res. 37, D832–836 (2009).
Jamain, S. et al. Mutations of the X- linked genes encoding neuroligins NLGN3 and NLGN4 are associated with autism. Nat. Genet. 34, 27–29 (2003).
Levy, D. et al. Rare de novo and transmitted copy-number variation in autistic spectrum disorders. Neuron 70, 886–897 (2011).
Földy, C., Malenka, R. C. & Südhof, T. C. Autism-associated neuroligin-3 mutations commonly disrupt tonic endocannabinoid signaling. Neuron 78, 498–509 (2013).
Sanders, S. J. et al. Insights into autism spectrum disorder genomic architecture and biology from 71 risk loci. Neuron 87, 1215–1233 (2015).
Ichtchenko, K. et al. Neuroligin 1: a splice site-specific ligand for β-neurexins. Cell 81, 435–443 (1995).
Ichtchenko, K., Nguyen, T. & Südhof, T. C. Structures, alternative splicing, and neurexin binding of multiple neuroligins. J. Biol. Chem. 271, 2676–2682 (1996).
Südhof, T. C. Neuroligins and neurexins link synaptic function to cognitive disease. Nature 455, 903–911 (2008).
Ylisaukko-oja, T. et al. Analysis of four neuroligin genes as candidates for autism. Eur. J. Human. Genet 13, 1285–1292 (2005).
Glessner, J. T. et al. Autism genome-wide copy number variation reveals ubiquitin and neuronal genes. Nature 459, 569–573 (2009).
Wöhr, M. et al. Developmental delays and reduced pup ultrasonic vocalizations but normal sociability in mice lacking the postsynaptic cell adhesion protein neuroligin2. Behav. Brain. Res. 251, 50–64 (2013).
Feng, J. et al. High frequency of neurexin 1β signal peptide structural variants in patients with autism. Neurosci. Lett. 409, 10–13 (2006).
Gauthier, J. et al. Truncating mutations in NRXN2 and NRXN1 in autism spectrum disorders and schizophrenia. Hum. Genet. 130, 563–573 (2011).
Vaags, A. K. et al. Rare deletions at the neurexin 3 locus in autism spectrum disorder. Am. J. Hum. Genet. 90, 133–141 (2012).
Durand, C. M. et al. Mutations in the gene encoding the synaptic scaffolding protein SHANK3 are associated with autism spectrum disorders. Nat. Genet. 39, 25–27 (2007).
Peça, J. et al. Shank3 mutant mice display autistic-like behaviours and striatal dysfunction. Nature 472, 437–442 (2011).
Sato, D. et al. SHANK1 deletions in males with autism spectrum disorder. Am. J. Hum. Genet. 90, 879–887 (2012).
Song, J.-Y., Ichtchenko, K., Südhof, T. C. & Brose, N. Neuroligin 1 is a postsynaptic cell-adhesion molecule of excitatory synapses. Proc. Natl Acad. Sci. USA 96, 1100–1105 (1999).
Varoqueaux, F., Jamain, S. & Brose, N. Neuroligin 2 is exclusively localized to inhibitory synapses. Eur. J. Cell. Biol. 83, 449–456 (2004).
Graf, E. R., Zhang, X., Jin, S.-X., Linhoff, M. W. & Craig, A. M. Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins. Cell 119, 1013–1026 (2004).
Levinson, J. N. et al. Neuroligins mediate excitatory and inhibitory synapse formation: involvement of PSD-95 and neurexin-1β in neuroligin-induced synaptic specificity. J. Biol. Chem. 280, 17312–17319 (2005).
Chih, B., Engelman, H. & Scheiffele, P. Control of excitatory and inhibitory synapse formation by neuroligins. Science 307, 1324–1328 (2005).
Chubykin, A. A. et al. Dissection of synapse induction by neuroligins: effect of a neuroligin mutation associated with autism. J. Biol. Chem. 280, 22365–22374 (2005).
Budreck, E. C. & Scheiffele, P. Neuroligin‐3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses. Eur. J. Neurosci. 26, 1738–1748 (2007).
Hoon, M. et al. Neuroligin-4 is localized to glycinergic postsynapses and regulates inhibition in the retina. Proc. Natl Acad. Sci. USA 108, 3053–3058 (2011).
Tabuchi, K. et al. A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice. Science 318, 71–76 (2007).
Etherton, M. et al. Autism-linked neuroligin-3 R451C mutation differentially alters hippocampal and cortical synaptic function. Proc. Natl Acad. Sci. USA 108, 13764–13769 (2011).
De Ferrari, G. V. & Moon, R. T. The ups and downs of Wnt signaling in prevalent neurological disorders. Oncogene 25, 7545–7553 (2006).
Okerlund, N. D. & Cheyette, B. N. Synaptic Wnt signaling—a contributor to major psychiatric disorders? J. Neurodev. Disord. 3, 162–174 (2011).
Kalkman, H. O. A review of the evidence for the canonical Wnt pathway in autism spectrum disorders. Mol. Autism 3, 10 (2012).
Krumm, N., O’Roak, B. J., Shendure, J. & Eichler, E. E. A de novo convergence of autism genetics and molecular neuroscience. Trends Neurosci. 37, 95–105 (2014).
Caracci, M. O., Ávila, M. E. & De Ferrari, G. V. Synaptic Wnt/GSK3β signaling hub in autism. Neural Plast. 2016, 9603751 (2016).
O’Roak, B. J. et al. Sporadic autism exomes reveal a highly interconnected protein network of de novo mutations. Nature 485, 246–250 (2012).
De Rubeis, S. et al. Synaptic, transcriptional and chromatin genes disrupted in autism. Nature 515, 209–215 (2014).
Hormozdiari, F., Penn, O., Borenstein, E. & Eichler, E. E. The discovery of integrated gene networks for autism and related disorders. Genome Res. 25, 142–154 (2015).
Wassink, T. H. et al. Evidence supporting WNT2 as an autism susceptibility gene. Am. J. Med. Genet. 105, 406–413 (2001).
Gharani, N., Benayed, R., Mancuso, V., Brzustowicz, L. M. & Millonig, J. H. Association of the homeobox transcription factor, ENGRAILED 2, 3, with autism spectrum disorder. Mol. Psychiatry 9, 474–484 (2004).
Boon, E. M., van der Neut, R., van de Wetering, M., Clevers, H. & Pals, S. T. Wnt signaling regulates expression of the receptor tyrosine kinase met in colorectal cancer. Cancer Res. 62, 5126–5128 (2002).
Campbell, D. B. et al. A genetic variant that disrupts MET transcription is associated with autism. Proc. Natl Acad. Sci. USA 103, 16834–16839 (2006).
Tuynman, J. B. et al. Cyclooxygenase-2 inhibition inhibits c-Met kinase activity and Wnt activity in colon cancer. Cancer Res. 68, 1213–1220 (2008).
Redies, C., Hertel, N. & Hübner, C. A. Cadherins and neuropsychiatric disorders. Brain. Res. 1470, 130–144 (2012).
O’Roak, B. J. et al. Exome sequencing in sporadic autism spectrum disorders identifies severe de novo mutations. Nat. Genet. 43, 585–589 (2011).
O’Roak, B. J. et al. Multiplex targeted sequencing identifies recurrently mutated genes in autism spectrum disorders. Science 338, 1619–1622 (2012).
Thompson, B. A., Tremblay, V., Lin, G. & Bochar, D. A. CHD8 is an ATP-dependent chromatin remodeling factor that regulates β-catenin target genes. Mol. Cell. Biol. 28, 3894–3904 (2008).
Hong, J. Y. et al. Down’s-syndrome-related kinase Dyrk1A modulates the p120-catenin–Kaiso trajectory of the Wnt signaling pathway. J. Cell. Sci. 125, 561–569 (2012).
de Ligt, J. et al. Diagnostic exome sequencing in persons with severe intellectual disability. N. Engl. J. Med. 367, 1921–1929 (2012).
Ashwood, P., Wills, S., Van & de Water, J. The immune response in autism: a new frontier for autism research. J. Leukoc. Biol. 80, 1–15 (2006).
Petrelli, F., Pucci, L. & Bezzi, P. Astrocytes and microglia and their potential link with autism spectrum disorders. Front Cell Neurosci. 10, 21 (2016).
Wei, H. et al. Brain IL-6 elevation causes neuronal circuitry imbalances and mediates autism-like behaviors. Biochim. Biophys. Acta 1822, 831–842 (2012).
Christensen, J. et al. Prenatal valproate exposure and risk of autism spectrum disorders and childhood autism. J. Assoc. Med. Assoc. 309, 1696–1703 (2013).
Moore, S. J. et al. A clinical study of 57 children with fetal anticonvulsant syndromes. J. Med. Genet. 37, 489–497 (2000).
Chen, G., Huang, L. D., Jiang, Y. M. & Manji, H. K. The mood-stabilizing agent valproate inhibits the activity of glycogen synthase kinase-3. J. Neurochem. 72, 1327–1330 (1999).
Phiel, C. J. et al. Histone deacetylase is a direct target of valproic acid, a potent anticonvulsant, mood stabilizer, and teratogen. J. Biol. Chem. 276, 36734–36741 (2001).
Mak, B. C., Takemaru, K.-I., Kenerson, H. L., Moon, R. T. & Yeung, R. S. The tuberin-hamartin complex negatively regulates β-catenin signaling activity. J. Biol. Chem. 278, 5947–5951 (2003).
Mak, B. C., Kenerson, H. L., Aicher, L. D., Barnes, E. A. & Yeung, R. S. Aberrant β-catenin signaling in tuberous sclerosis. Am. J. Pathol. 167, 107–116 (2005).
Ashwood, P. & Wakefield, A. J. Immune activation of peripheral blood and mucosal CD3+ lymphocyte cytokine profiles in children with autism and gastrointestinal symptoms. J. Neuroimmunol. 173, 126–134 (2006).
Chez, M. G., Dowling, T., Patel, P. B., Khanna, P. & Kominsky, M. Elevation of tumor necrosis factor-alpha in cerebrospinal fluid of autistic children. Pediatr. Neurol. 36, 361–365 (2007).
Croonenberghs, J., Bosmans, E., Deboutte, D., Kenis, G. & Maes, M. Activation of the inflammatory response system in autism. Neuropsychobiology 45, 1–6 (2002).
Jyonouchi, H., Sun, S. & Le, H. Proinflammatory and regulatory cytokine production associated with innate and adaptive immune responses in children with autism spectrum disorders and developmental regression. J. Neuroimmunol. 120, 170–179 (2001).
Molloy, C. A. et al. Elevated cytokine levels in children with autism spectrum disorder. J. Neuroimmunol. 172, 198–205 (2006).
Singh, V. K. Plasma increase of interleukin-12 and interferon-gamma. Pathological significance in autism. J. Neuroimmunol. 66, 143–145 (1996).
Tsilioni, I., Taliou, A., Francis, K. & Theoharides, T. C. Children with autism spectrum disorders, who improved with a luteolin-containing dietary formulation, show reduced serum levels of TNF and IL-6. Transl. Psychiatry 5, e647 (2015).
Vargas, D. L., Nascimbene, C., Krishnan, C., Zimmerman, A. W. & Pardo, C. A. Neuroglial activation and neuroinflammation in the brain of patients with autism. Ann. Neurol. 57, 67–81 (2005).
Li, X. et al. Elevated immune response in the brain of autistic patients. J. Neuroimmunol. 207, 111–116 (2009).
Zolezzi, J. M. & Inestrosa, N. C. Wnt/TLR dialog in neuroinflammation, relevance in alzheimer’s disease. Front. Immunol. 8, 187 (2017).
Marchetti, B. & Pluchino, S. Wnt your brain be inflamed? Yes, it Wnt! Trends Mol. Med. 19, 144–156 (2013).
Halleskog, C. et al. WNT signaling in activated microglia is proinflammatory. GLIA 59, 119–131 (2011).
Hödar, C. et al. Genome-wide identification of new Wnt/β-catenin target genes in the human genome using CART method. BMC Genom. 11, 1 (2010).
Wexler, E. M. et al. Genome-wide analysis of a Wnt1-regulated transcriptional network implicates neurodegenerative pathways. Sci. Signal. 4, ra65 (2011).
Wisniewska, M. B., Nagalski, A., Dabrowski, M., Misztal, K. & Kuznicki, J. Novel β-catenin target genes identified in thalamic neurons encode modulators of neuronal excitability. BMC Genom. 13, 635 (2012).
Perez-Palma, E. et al. Early transcriptional changes induced by Wnt/β-catenin signaling in hippocampal neurons. Neural Plast. 2016, 4672841 (2016).
Maretto, S. et al. Mapping Wnt/β-catenin signaling during mouse development and in colorectal tumors. Proc. Natl Acad. Sci. USA 100, 3299–3304 (2003).
Harrison-Uy, S. J. & Pleasure, S. J. Wnt signaling and forebrain development. Cold Spring Harb. Perspect. Biol. 4, a008094 (2012).
Chen, J., Park, C. S. & Tang, S. J. Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation. J. Biol. Chem. 281, 11910–11916 (2006).
Cerpa, W. et al. Wnt-7a modulates the synaptic vesicle cycle and synaptic transmission in hippocampal neurons. J. Biol. Chem. 283, 5918–5927 (2008).
Avila, M. E. et al. Canonical Wnt3a modulates intracellular calcium and enhances excitatory neurotransmission in hippocampal neurons. J. Biol. Chem. 285, 18939–18947 (2010).
Sharma, K. et al. High-throughput genetic screen for synaptogenic factors: identification of LRP6 as critical for excitatory synapse development. Cell Rep. 5, 1330–1341 (2013).
De Ferrari, G. V. et al. Activation of Wnt signaling rescues neurodegeneration and behavioral impairments induced by β-amyloid fibrils. Mol. Psychiatry 8, 195–208 (2003).
Frederiksen, K., Jat, P. S., Valtz, N., Levy, D. & McKay, R. Immortalization of precursor cells from the mammalian CNS. Neuron 1, 439–448 (1988).
Morimoto, B. H. & Koshland, D. E. Jr. Induction and expression of long- and short-term neurosecretory potentiation in a neural cell line. Neuron 5, 875–880 (1990).
De Ferrari, G. V. et al. Common genetic variation within the low-density lipoprotein receptor-related protein 6 and late-onset Alzheimer’s disease. Proc. Natl Acad. Sci. USA 104, 9434–9439 (2007).
Vanderweyde, T. et al. Interaction of tau with the RNA-binding protein TIA1 regulates tau pathophysiology and toxicity. Cell Rep. 15, 1455–1466 (2016).
Willert, K. et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423, 448–452 (2003).
Kishida, S., Yamamoto, H. & Kikuchi, A. Wnt-3a and Dvl induce neurite retraction by activating Rho- associated kinase. Mol. Cell Biol. 24, 4487–4501 (2004).
Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671–675 (2012).
Nunez, F., Bravo, S., Cruzat, F., Montecino, M. & De Ferrari, G. V. Wnt/β-catenin signaling enhances cyclooxygenase-2 (COX2) transcriptional activity in gastric cancer cells. PLoS ONE 6, e18562 (2011).
Ugarte, G. D. et al. Wnt signaling induces transcription, spatial proximity, and translocation of fusion gene partners in human hematopoietic cells. Blood 126, 1785–1789 (2015).
Alarcon, M. A. et al. A novel functional low-density lipoprotein receptor-related protein 6 gene alternative splice variant is associated with Alzheimer’s disease. Neurobiol. Aging 34, e1709–1718 (2013).
Biechele, T. L. & Moon, R. T. Assaying β-catenin/TCF transcription with β-catenin/TCF transcription-based reporter constructs. Methods Mol. Biol. 468, 99–110 (2008).
Korinek, V. et al. Constitutive transcriptional activation by a β-catenin-Tcf complex in APC-/- colon carcinoma. Science 275, 1784–1787 (1997).
Klein, P. S. & Melton, D. A. A molecular mechanism for the effect of lithium on development. Proc. Natl Acad. Sci. USA 93, 8455–8459 (1996).
Lian, X. et al. Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling. Stem Cell Rep. 3, 804–816 (2014).
Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676–682 (2012).
Sage, D. et al. DeconvolutionLab2: an open- source software for deconvolution microscopy. Methods 115, 28–41 (2017).
Lazo, O. M. et al. BDNF regulates Rab11- mediated recycling endosome dynamics to induce dendritic branching. J. Neurosci. 33, 6112–6122 (2013).
Yochum, G. S. et al. Serial analysis of chromatin occupancy identifies β-catenin target genes in colorectal carcinoma cells. Proc. Natl Acad. Sci. USA 104, 3324–3329 (2007).
Hatzis, P. et al. Genome-wide pattern of TCF7L2/TCF4 chromatin occupancy in colorectal cancer cells. Mol. Cell. Biol. 28, 2732–2744 (2008).
Bottomly, D., Kyler, S. L., McWeeney, S. K. & Yochum, G. S. Identification of β-catenin binding regions in colon cancer cells using ChIP-Seq. Nucleic Acids Res. 38, 5735–5745 (2010).
Ovcharenko, I., Nobrega, M. A., Loots, G. G. & Stubbs, L. ECR Browser: a tool for visualizing and accessing data from comparisons of multiple vertebrate genomes. Nucleic Acids Res. 32, W280–286 (2004).
Medina, M. A. et al. Alternative RUNX1 promoter regulation by Wnt/β-catenin signaling in leukemia cells and human hematopoietic progenitors. J. Cell. Physiol. 231, 1460–1467 (2016).
He, T.-C. et al. Identification of c-MYC as a target of the APC pathway. Science 281, 1509–1512 (1998).
Hutsler, J. J. & Zhang, H. Increased dendritic spine densities on cortical projection neurons in autism spectrum disorders. Brain. Res. 1309, 83–94 (2010).
Tang, G. et al. Loss of mTOR- dependent macroautophagy causes autistic-like synaptic pruning deficits. Neuron 83, 1131–1143 (2014).
Zoghbi, H. Y. & Bear, M. F. Synaptic dysfunction in neurodevelopmental disorders associated with autism and intellectual disabilities. Cold Spring Harb. Perspect. Biol. 4, a009886 (2012).
Gujral, T. S. & MacBeath, G. A system-wide investigation of the dynamics of Wnt signaling reveals novel phases of transcriptional regulation. PLoS ONE 5, e10024 (2010).
Tiwari, S. K. et al. Curcumin-loaded nanoparticles potently induce adult neurogenesis and reverse cognitive deficits in Alzheimer’s disease model via canonical Wnt/β-catenin pathway. ACS Nano 8, 76–103 (2014).
Bhandari, R. & Kuhad, A. Neuropsychopharmacotherapeutic efficacy of curcumin in experimental paradigm of autism spectrum disorders. Life. Sci. 141, 156–169 (2015).
Al-Askar, M., Bhat, R. S., Selim, M., Al-Ayadhi, L. & El-Ansary, A. Postnatal treatment using curcumin supplements to amend the damage in VPA-induced rodent models of autism. BMC Complement. Altern. Med. 17, 259 (2017).
Lopresti, A. L. Curcumin for neuropsychiatric disorders: a review of in vitro, animal and human studies. J. Psychopharmacol. 31, 287–302 (2017).
Lie, D.-C. et al. Wnt signalling regulates adult hippocampal neurogenesis. Nature 437, 1370–1375 (2005).
Hollis, E. R. II & Zou, Y. Expression of the Wnt signaling system in central nervous system axon guidance and regeneration. Front Mol. Neurosci. 5, 5 (2012).
Takeichi, M. & Abe, K. Synaptic contact dynamics controlled by cadherin and catenins. Trends Cell. Biol. 15, 216–221 (2005).
Ahmad-Annuar, A. et al. Signaling across the synapse: a role for Wnt and Dishevelled in presynaptic assembly and neurotransmitter release. J. Cell. Biol. 174, 127–139 (2006).
Ciani, L. et al. Wnt7a signaling promotes dendritic spine growth and synaptic strength through Ca2+/Calmodulin-dependent protein kinase II. Proc. Natl Acad. Sci. USA 108, 10732–10737 (2011).
Gao, X., Arlotta, P., Macklis, J. D. & Chen, J. Conditional knock-out of β-catenin in postnatal-born dentate gyrus granule neurons results in dendritic malformation. J. Neurosci. 27, 14317–14325 (2007).
Huang, C. W. et al. Conditional knockout of breast carcinoma amplified sequence 2 (BCAS2) in mouse forebrain causes dendritic malformation via β-catenin. Sci. Rep. 6, 34927 (2016).
Yu, X. & Malenka, R. C. Beta-catenin is critical for dendritic morphogenesis. Nat. Neurosci. 6, 1169–1177 (2003).
De Roo, M., Klauser, P., Mendez, P., Poglia, L. & Muller, D. Activity-dependent PSD formation and stabilization of newly formed spines in hippocampal slice cultures. Cereb. Cortex. 18, 151–161 (2008).
Guo, W., Ceolin, L., Collins, K. A., Perroy, J. & Huber, K. M. Elevated CaMKIIalpha and hyperphosphorylation of homer mediate circuit dysfunction in a fragile X syndrome mouse model. Cell Rep. 13, 2297–2311 (2015).
Stephenson, J. R. et al. A novel human CAMK2A mutation disrupts dendritic morphology and synaptic transmission, and causes ASD-related behaviors. J. Neurosci. 37, 2216–2233 (2017).
Gibson, E. M. et al. Neuronal activity promotes oligodendrogenesis and adaptive myelination in the mammalian brain. Science 344, 1252304 (2014).
Venkatesh, H. S. et al. Neuronal activity promotes glioma growth through neuroligin-3 secretion. Cell 161, 803–816 (2015).
Rubenstein, J. L. & Merzenich, M. M. Model of autism: increased ratio of excitation/inhibition in key neural systems. Genes. Brain. Behav. 2, 255–267 (2003).
Nelson, S. B. & Valakh, V. Excitatory/inhibitory balance and circuit homeostasis in autism spectrum disorders. Neuron 87, 684–698 (2015).
Acknowledgements
We are grateful to Felipe Nuñez (PhD), Marleen Duivenvoorden (MD) and James Motta for preliminary experiments. This study was supported by FONDECYT grants from CONICYT- Chile 1140353 to G.V.D., 1150816 to A.E.R., 3150612 to M.F.V. and 3130509 to G.D.U.
Author information
Authors and Affiliations
Contributions
M.A.M., G.D.U., and G.V.D. conceived the project and designed the experiments. M.A.M., V.M.A., M.O.C., D.A.V., M.E.A., M.F.V., and G.D.U. performed experiments or analyzed the data. C.O. and A.E.R. analyzed the data. M.A.M., M.O.C., G.D.U., and G.V.D. wrote the paper.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Medina, M.A., Andrade, V.M., Caracci, M.O. et al. Wnt/β-catenin signaling stimulates the expression and synaptic clustering of the autism-associated Neuroligin 3 gene. Transl Psychiatry 8, 45 (2018). https://doi.org/10.1038/s41398-018-0093-y
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41398-018-0093-y
- Springer Nature Limited
This article is cited by
-
Neurodevelopmental delay in children exposed to maternal SARS-CoV-2 in-utero
Scientific Reports (2024)
-
Neuroligin 2 governs synaptic morphology and function through RACK1-cofilin signaling in Drosophila
Communications Biology (2023)
-
Impaired cecal motility and secretion alongside expansion of gut-associated lymphoid tissue in the Nlgn3R451C mouse model of autism
Scientific Reports (2023)
-
A perspective on molecular signalling dysfunction, its clinical relevance and therapeutics in autism spectrum disorder
Experimental Brain Research (2022)
-
Overlapping Molecular Pathways Leading to Autism Spectrum Disorders, Fragile X Syndrome, and Targeted Treatments
Neurotherapeutics (2021)