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Electroporation Increases DNA Synthesis in Cultured Plant Protoplasts

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Abstract

DNA synthesis increased significantly in isolated Prunus and Solanumprotoplasts electroporated at 250V cm−1 for 87 μsec and 750V cm−1 for 29 μsec respectively. This increase correlates with an earlier onset of division of protoplast-derived cells in culture. Inhibition of cell wall synthesis did not affect electro-enhancement of DNA synthesis. Electrostimulation of DNA synthesis coupled with enhanced division may have applications to protoplast systems and plant genetic engineering in general.

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, Nottingham, NG7 2RD, U.K.

    Michael R. Davey, Bernard J. Mulligan & John B. Power

  2. Centre Nacional de Recursos Geneticos e Biotecnologia (EMBRAPA), Brasilia, Brazil

    Elibio L. Rech

  3. Centro de Ecofisiologia Vegetal (CONICET), Buenos Aires, Argentina

    Sergio J. Ochatt

  4. Post Graduate Department of Botany, Utkal University, Bhubaneshwar, India

    Pradeep K. Chand

Authors
  1. Elibio L. Rech
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  2. Sergio J. Ochatt
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  3. Pradeep K. Chand
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  4. Michael R. Davey
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  5. Bernard J. Mulligan
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  6. John B. Power
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Rech, E., Ochatt, S., Chand, P. et al. Electroporation Increases DNA Synthesis in Cultured Plant Protoplasts. Nat Biotechnol 6, 1091–1093 (1988). https://doi.org/10.1038/nbt0988-1091

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  • DOI: https://doi.org/10.1038/nbt0988-1091

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