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Cloning A Streptomyces clavuligerus Genetic Locus Involved in Clavulanic Acid Biosynthesis

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Abstract

The multi-copy plasmid vector pIJ702 has been used to clone Streptomyces clavuligerus chromosomal DNA fragments into a clavulanic acid non-producing mutant strain. Two DNA segments, which restored the ability to synthesise clavulanic acid, were shown to overlap one another and share a common sequence of about 2 kb. A preliminary restriction map of the region of the chromosome involved has been constructed.

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Author notes

  1. Christopher R. Bailey and David J. Winstanley: Beecham Pharmaceuticals Research Division, Brockham Park, Betchworth, Surrey, RH3 7AJ, England

  2. Michael J. Butler: Pfizer Limited, Ramsgate Road, Sandwich, Kent, CT13 9NJ, England

  3. Robert T. Rowlands: Panlab Genetics, Inc., 110-110th Avenue N.E., Suite 704, Bellevue, WA 98004, USA

Authors and Affiliations

  1. Beecham Pharmaceuticals UK Division, Clarendon Road, Worthing, West Sussex, BN14 8QH, England

    Ian D. Normansell

Authors
  1. Christopher R. Bailey
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  2. Michael J. Butler
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  3. Ian D. Normansell
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  4. Robert T. Rowlands
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  5. David J. Winstanley
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Bailey, C., Butler, M., Normansell, I. et al. Cloning A Streptomyces clavuligerus Genetic Locus Involved in Clavulanic Acid Biosynthesis. Nat Biotechnol 2, 808–811 (1984). https://doi.org/10.1038/nbt0984-808

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  • DOI: https://doi.org/10.1038/nbt0984-808

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