Abstract
Polyhedral oligomeric silsesquioxane (POSS) is a unique molecule that is composed of an inorganic silica core with eight organic functional arms, which can be used as a nucleus for covalent bonding to create star-shaped block copolymers with improved mechanical and biological properties. In this work, highly porous star-shaped POSS-polycaprolactone-polyurethane (POSS-PCL-PU) films were synthesized as scaffold biomaterials for tissue engineering. These films have an interesting morphology consisting of rough spherulites with filamentous structures spreading out from their centers; the unique nanotopography was shown to be suitable for cell growth. In vitro degradation was monitored for 52 weeks in terms of the weight loss and morphology changes of the films. The degradation profile exhibited a slow initial weight loss of <1% during the first 24 weeks, followed by a rapid weight loss of ~18% in the following 28 weeks. The films demonstrated excellent biocompatibility and cell-substrate affinity, with a high cell viability of >95% and rapid cell proliferation. The high porosity, unique surface nanotopography and excellent biocompatibility of the star-shaped POSS-PCL-PU film make it a great candidate as a tissue engineering scaffold biomaterial.
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Introduction
Tissue engineering has emerged as a promising treatment to improve or replace the biological functions of the damaged tissue or organs. Tissue engineering involves the use of a combination of cells, engineered materials and suitable biochemical factors for the growth of donor or patient cells in a three-dimensional scaffold that acts as a structural template upon which cells can adhere, proliferate and eventually form new tissues.1, 2 Ideally, scaffolds should be biocompatible to prevent inflammation or toxicity reactions in vivo, and they should also be highly porous to allow for the cellular infiltration and nutrition supply via diffusion, convection and directed fluid flow during the culture.3 It is advantageous when scaffolds have a structure similar to the extracellular matrix for cell guidance and cell–matrix interactions to facilitate neotissue development.4 It is also important to have controlled degradation rates to provide the required support for tissue formation, allowing degradation to start as neotissue forms. The products of degradation should be biocompatible to ensure that there is no toxicity.5
Block copolymers are attractive scaffold materials because their properties, such as porosity, biodegradability and mechanical strength, can be appropriately designed and specifically tuned to match the required tissue engineering application.6 Polyhedral oligomeric silsesquioxane (POSS) is composed of an inorganic silica cube formed by rigid Si–O bonds with eight organic functional arms7, 8, 9, 10, 11 that are reactive with other polymers via covalent bonding to form hybrid star-shaped block copolymers.12 POSS-based materials exhibit enhanced thermal and mechanical stability arising from the inorganic properties and structural features of the POSS core. In addition, these hybrid materials also offer great versatility and can be used in the development of advanced biomaterials.11 For example, POSS can be incorporated with polycaprolactone (PCL),13 polyurethane (PU),14 polycarbonate urethane urea15 and polycarbonate urethane16 for improved biological and mechanical properties to achieve better processability, increased strength, toughness and higher stability for tissue engineering applications.17 Oseni et al16 reported that the surface topography of POSS enabled nanoscale interactions of cells via a scaffold matrix, which increased the viability of chondrocytes on POSS-polycarbonate urethane scaffolds compared with PCL scaffolds. Knight et al18 reported that there was minimal weight loss for POSS-incorporated polyester PU films in the earlier stage, followed by a large mass loss at the later stage of in vitro degradation, and the hybrid films also exhibited excellent biocompatibility with no inflammatory responses. POSS-PU hybrids were reported19, 20, 21 to possess improved properties, such as higher thermal stability, higher oxidative resistance and increased mechanical strength.
In this study, we report the use of a star-shaped POSS-PCL-PU polymer as a scaffold material in tissue engineering applications. POSS was used as the starting inorganic core to introduce three-dimensionality and to improve the surface nanotopography. By incorporating organic groups in the robust core through extension with PCL and cross-linking with PU, the resulting unique inorganic–organic nanocomposite can achieve various functionalities, such as enhanced cell attachment, biodegradability and stability of the overall structure. The star-shaped POSS-PCL-PU films were investigated regarding their properties, such as porosity, hydrophobicity and surface topography. In addition, degradation studies and cytotoxicity and biocompatibility tests were performed to access the suitability of the films as tissue engineering scaffolds. The POSS-PCL-PU films exhibit a high porosity of >75% and intrinsic nanoscale features that enhance cell attachment and growth. The films exhibited negligible weight loss during the initial 24 weeks of degradation, followed by a large increase in the weight loss of 18% in the following 28 weeks. Most importantly, the POSS-PCL-PU films are highly biocompatible, as cell viability is still maintained at >95%, even after 48 h of incubation with the byproducts of degradation.
Materials and methods
Materials
POSS (POSS, Mw=1017.99) was purchased from Hybrid Plastics Inc. (Hattiesburg, MS, USA) and dried under vacuum at 60 °C for 24 h prior to use. ɛ-Caprolactone was purchased from Tokyo Chemical Industry (Tokyo, Japan) and distilled over calcium hydride under reduced pressure prior to use. PCL (Mw=80 000), allyl alcohol (⩾99%), hexamethylene diisocyanate (99%), stannous octoate (95%), dibutyltin dilaurate (95%) and fluorescein diacetate were obtained from Sigma-Aldrich (St Louis, MO, USA). alamarBlue cell viability assay was purchased from Life Technologies (Grand Island, NY, USA). Platinum divinyltetramethyldisiloxane was supplied by Aldrich and diluted to 2 mM solution in anhydrous toluene for use as a Karstedt’s catalyst. Toluene was freshly distilled over sodium benzophenone under nitrogen prior to use. The other chemicals were used as received without further purification.
Synthesis of star-shaped POSS-PCL
Hydroxyl terminated POSS (POSS-OH) was first synthesized with the use of allyl alcohol as the monomer and platinum divinyltetramethyldisiloxane as a Karstedt’s catalyst according to previous reports.18, 19, 20, 21 The proton nuclear magnetic spectrum of POSS-OH is shown in Supplementary Figure S1a. The disappearance of the Si–H proton resonance peak at 4.72 p.p.m in proton nuclear magnetic resonance indicated that all eight corners of the POSS core were substituted with the hydroxyl group. A batch of star-shaped POSS-PCL was then synthesized by using a 1:480 ratio of POSS-OH to ɛ-caprolactone. In a typical procedure, POSS-OH (0.1 g, 0.07 mmol), ɛ-caprolactone (3.84 g, 33.60 mmol) and stannous octoate (3.72 mg, 0.01 mmol) were mixed into a predried Schlenk flask followed by five cycles of freeze–pump–thaw degassing of the mixture under magnetic stirring. The flask was then sealed and immersed in a thermostat oil bath at 120 °C for 12 h. The crude product was dissolved in a minimum amount of chloroform and then precipitated in an excess amount of cold methanol. After repeating the precipitation process three times, the obtained product was dried in a vacuum oven overnight at 40 °C with an yield of >98%. The as-prepared POSS-PCL was characterized via measurement of the proton nuclear magnetic resonance spectrum, as shown in Supplementary Figure S1b. Proton nuclear magnetic spectrum (CDCl3, 400 MHz, p.p.m): 0.14 (Si(CH3)2CH2); 0.61 (Si(CH3)2CH2); 1.68 (Si(CH3)2CH2CH2CH2–PCL); 4.06 (O–CO–((CH2)4–CH2–)n); 3.65 (O–CO–(CH2)4–CH2–OH); 2.26–2.30 (O–CO–CH2–(CH2)4); 1.60–1.68 (O–CO–CH2CH2–(CH2)3); and 1.34–1.41 (O–CO– CH2CH2CH2CH2CH2). In our star-shaped POSS-PCL polymer, the integral ratio of –Si(CH3)2 to –Si(CH3)2CH2 is 5.9:2.0, which is close to the theoretical ratio of 6:2. There is a higher proportion of –CH2–OH compared with –Si(CH3)2CH2, with an integral ratio of 2.8:2.0, compared with the theoretical ratio of 2:2, suggesting the presence of unattached sole PCL chains in the star-shaped POSS-PCL. Nevertheless, the majority of the PCL chains were attached to the POSS core to achieve a star-shaped polymer, as shown by the sharp melting transition in differential scanning calorimetry thermogram,12 which is ascribed to the formation of a homogeneously cross-linked structure from the POSS core containing uniformly distributed polymer chains.
Preparation of star-shaped POSS-PCL-PU films and linear PCL-PU films
To synthesize star-shaped POSS-PCL-PU films, a stoichiometric amount of hexamethylene diisocyanate was added into a flask containing 5 g of as-purified POSS-PCL dissolved in 25 ml of freshly dried chloroform (the end group, –OH of POSS-PCL reacted with hexamethylene diisocyanate to form the –NHCO functional group of urethane). The mixed solution was stirred to react for 6 h at room temperature after adding dibutyltin dilaurate as the catalyst. The solution was then poured into a polytetrafluoroethylene mold, followed by placing the mold containing the solution into a nitrogen-filled oven at room temperature for 24 h prior to curing at 80 °C for 12 h. The resulting film was washed with chloroform to remove the materials that were not cross-linked, and then the washed film was dried in a vacuum oven at 80 °C to obtain star-shaped POSS-PCL-PU films. The samples were then cut into small square pieces with dimensions of 10 mm × 10 mm × 1 mm. As a comparative study, linear PCL-PU was also prepared by mixing PCL and hexamethylene diisocyanate at a stoichiometric ratio and stirring for 6 h at room temperature, followed by performing the same curing and washing process described above. The linear PCL-PU films were also cut into square pieces of the same dimensions as POSS-PCL-PU films.
Characterization
A JEOL 5600 scanning electron microscope (SEM) (JEOL, Tokyo, Japan) was used to characterize the surface morphology of the films. To further analyze the surface, atomic force microscopy (AFM) was performed using a Dimension ICON AFM (Bruker, Santa Barbara, CA, USA) operated in tapping mode at a scan frequency of 0.5 Hz, using standard point probes (Nanoworld, Neuchâtel, Switzerland). Hydrophobicity of the films was determined by measuring the contact angle using NRL C.A Goniometer, Model No.100-00 from Ramé-hart (Succasunna, NJ, USA). A drop of water was dropped onto the surface of the films, and contact angle was measured from both edges of the droplet. The average contact angle was determined from 10 readings.
Measurement of film porosity
The liquid displacement method was chosen to determine the porosity of the polymer films.22, 23 The criterion for the displacement liquid is that it should prevent shrinkage or swelling of the material, so that there is no change in the volume of material. In addition, the material should not dissolve in the displacement liquid. Distilled water was selected as the displacement liquid because it can easily enter the pores of the polymer sample and it does not cause changes in the size of the material. A measuring cylinder with 20 ml of the displacement liquid was weighed and denoted as M1. A piece of film of known weight (Ms) was transferred into the cylinder and sonicated (Elma Transsonic Digitals Ultrasonic bath (Hilsonic, Birkenhead, UK), 100% power) for 20 min to facilitate permeation of the liquid into the pores. After standing overnight, the liquid was topped up to the original level of 20 ml and weighed (M2). Subsequently, the polymer film was removed, and then the cylinder was weighed (M3). The porosity was calculated using (M2−M3−Ms)/(M1−M3).
Weight loss and morphology changes during in vitro degradation
The initial weights (Mi) of star-shaped POSS-PCL-PU and linear PCL-PU films with dimensions of 10 mm × 10 mm × 1 mm were measured. Each individual piece was placed into a vial containing 5 ml of phosphate buffered saline (PBS) buffer. The bottles were subsequently transferred into a water bath (Grant OLS200), with continuous shaking at 100 r.p.m at a temperature of 37 °C for 52 weeks. The samples were taken out at predetermined time points and rinsed with distilled water and then freeze-dried for 24 h before measuring their final weights (Mf). The percentage weight loss was calculated using (Mi−Mf)/Mi × 100%. The samples were sputter coated with gold (JEOL JFC-1200 fine coater) (JEOL) for 20 s and then viewed using SEM to observe the morphology changes due to degradation.
Cell viability
Five pieces of POSS-PCL-PU or PCL-PU films with a total weight of ~24 mg were incubated in 2 ml of PBS buffer at 37 °C for 8 weeks under shaking at 100 r.p.m. After removing the films, a solution at a concentration of 12.0 mg ml−1 was obtained and then further diluted into different concentrations (6.0, 3.0, 1.5 and 0.75 mg ml−1). Fibroblast cells (CCD-112 CoN) were seeded at a density of 2.0 × 104 cells cm−2 in 96-well plates (Corning) and then incubated with the above solutions for 48 h at 37 °C in a humidified atmosphere containing 5% CO2. After incubation, the viability of the cells was evaluated via alamarBlue (Life Technologies) reagent. The reducing environment of the viable cells converts the blue resazurin contents of alamarBlue (Life Technologies) reagent into highly fluorescent red resorufin, which can be measured using 570 nm excitation by a Tecan microplate reader. An average of five readings was taken for each concentration. The viability is calculated by the fluorescence emission values at 610 nm of the treated samples divided by that of the control cells without treatment and presented as a percentage viability of the control cells.
Confocal imaging
The films were washed with ethanol and double-distilled water three times before use in the cell culture. The fibroblast cells were first seeded on the POSS-PCL-PU and PCL-PU films and further incubated at 37 °C for 10 days. The culture medium was changed every alternate day. At the end of the incubation period, the cells were stained with 1 μg ml−1 of fluorescein diacetate for 20 min and then rinsed with PBS. Cell adhesion and proliferation were determined using a confocal microscope (FV1000 system, Olympus, Tokyo, Japan) equipped with a 20 × dry objective. Z-stack images of 1024 × 1024 pixels were acquired with 1.5-μm incremental steps over 0.0–15.0 μm in the z direction and processed using Olympus Fluoview (Olympus) and Imaris from Bitplane (Zurich, Switzerland). Imaris is an imaging software that allows for three-dimensional rendering and cross-sectional viewing of confocal images.
Results and Discussion
POSS-PCL was synthesized by reacting POSS-OH with ɛ-caprolactone extension, followed by a reaction with hexamethylene diisocyanate to form the star-shaped POSS-PCL-PU (Figure 1). By incorporating POSS in PCL-PU, which is a commonly used material in tissue engineering, the functionalities can be tailored to achieve the desired properties. To compare the differences in characteristics because of the incorporation of POSS in this nanocomposite, PCL-PU was selected as a control. Both star-shaped POSS-PCL-PU and linear PCL-PU films were characterized to determine their morphology, porosity and hydrophobicity. These films were also evaluated regarding their biodegradability, growth of cells and cytotoxicity of their degradation products. It was observed via SEM that star-shaped POSS-PCL-PU films presented tightly packed spherulites of 50–70 μm in size with some gaps in between (Figures 2a and b). These spherulites were rough with filamentous networks branching outwards in the radial direction from the center, revealing the three-dimensional structures introduced by the POSS core. The SEM analysis also revealed that the film morphology was homogenous, affirming that it is composed of mainly of POSS-PCL-PU. There was no noticeable amount of PCL-PU formed from the unattached PCL, which is postulated to be present in the mixture of POSS-PCL. In comparison, the linear PCL-PU films featured tightly packed spherulites of 40–50 μm in size (Figures 2d and e), which were two-dimensional and smooth. The regions around the peripheries of spherulites were porous, as highlighted by the red circles in Figure 2e. It was also observed via AFM that the POSS-PCL-PU films had a rough surface with a large number of nanosize peaks (Figure 2c), but the PCL-PU films did not exhibit these nanofeatures (Figure 2f). The unique morphology and nanorough surface of the star POSS-PCL-PU films observed via SEM and AFM can be attributed to the three-dimensional structure of the POSS core and the synergy of the hydrophobic moieties of POSS in combination with the PCL-PU morphology. The rough surface features of the POSS-PCL-PU films have favorable effects on cell response.16, 24
The porosity of the two types of films was characterized using the liquid displacement method. Star-shaped POSS-PCL-PU films possessed a higher porosity of 75.7±7.4% compared with the porosity of the linear PCL-PU films of 43.1±4.4%. The significantly higher porosity with greater interconnectivity of pores enables more efficient transport of nutrients in the scaffolds without compromising the mechanical stability. Their hydrophobicity was further assessed to verify the wettability of the films by using a contact angle goniometer. The star-shaped POSS-PCL-PU films were found to be slightly hydrophilic, with a contact angle of 89°, while the linear PCL-PU films were found to be hydrophobic, with a contact angle of 98°. The POSS moieties have weak polarity, because of its enclosed cubic silica structure, and are distinctly hydrophobic.8, 25 However, this hydrophobic nature of POSS was not observed in the star POSS-PCL-PU films. The reason for the slight hydrophilicity observed in the star-shaped POSS-PCL-PU films could be because of their higher porosity, thereby causing more water to be adsorbed at the surface and resulting in a decrease in the wettability of films, which further affects the cell adhesion. A similar finding was observed earlier in POSS-polycarbonate urethane foam matrices,16 and the increased hydrophilicity with higher POSS content was attributed to the increased porosity and pore size.
To evaluate the feasibility of using star-shaped POSS-PCL-PU as a promising scaffold material for tissue engineering, it is important to study their degradation profiles to determine the suitable bioapplications. Hence, degradation studies were performed on star-shaped POSS-PCL-PU and linear PCL-PU films over 52 weeks. Figure 3 shows the weight loss profiles of the two films in PBS buffer at 37 °C. For the POSS-PCL-PU films, although there was a negligible weight loss of <1% in the initial 24 weeks of degradation, substantial weight loss of ~9% was observed at 36 weeks, and a further 9% weight loss was observed at the end of 52 weeks. The slow initial rate of hydrolytic degradation can be explained by the hydrophobic nature and the shielding effect of the POSS moieties.26 The rate of degradation increased by many fold only after the POSS moieties were broken down. The degradation rate can be determined by varying POSS content; a higher POSS content corresponds to a slower degradation rates.27 Together with the mechanical integrity, the degradation rate of the POSS-PCL-PU films can be controlled to achieve a scaffold that degrades slowly during the initial period, followed by rapid degradation at the later phase after the growth of the cells. This behavior is desired because the scaffold’s structural integrity is required in the initial phase for cells to attach and proliferate. At the later phase, as the cells have grown to form tissues, the scaffold has fulfilled its intended function and should be quickly eliminated from body. In comparison, linear PCL-PU films exhibited a gradual weight loss over 52 weeks. The weight loss profile of the PCL-PU films was observed to be linear and consistent, while that of POSS-PCL-PU films was found to be biphasic because of the protective effect of the POSS cages during the initial phase. The overall weight loss of the PCL-PU films at the end of 52 weeks was low, at a~11%, which is because of the hydrophobicity and the semi-crystalline structure of the PCL backbone, resulting in slow degradation.28 However, PCL-PU copolymers were reported to be stiff and hydrophobic, causing compatibility issues with soft tissues, thereby limiting their use in tissue engineering applications.29
The morphology of the degraded films was also monitored using an SEM during the in vitro degradation for 52 weeks (Figure 4). The morphological changes of the star-shaped POSS-PCL-PU and the linear PCL-PU films were found to be minimal in the first 24 weeks. For the star-shaped POSS-PCL-PU film (Figures 4a and e), the surface of the spherulites became less smooth, and the gaps between spherulites disappeared at 4 weeks of degradation. After 4 weeks, more tiny particles appeared on the surface with increased time. For the linear PCL-PU film (Figures 4f and j), the surface of spherulites still remained smooth in the first 4 weeks, and then cracks were formed on the surface after week 24. At higher magnifications, smaller cracks were observed in the POSS-PCL-PU film (Figures 5a and b) and the PCL-PU film (Figures 5c and d) after the 24 weeks of degradation. The porous regions were found to become separate fibril strands in the PCL-PU film (Figure 5d). At the end of 52 weeks, the distinct shape of spherulites was no longer observed, whereas more and larger cracks were observed on the partially degraded film. During the later phase of the study, both films became more brittle and broke into tiny fragments easily during handling because of the reduced molecular weight of polymeric films after degradation.
After incubating 24 mg of POSS-PCL-PU or PCL-PU films in 2 ml of PBS at 37 °C for 8 weeks, the degraded products from the films were extracted into PBS and used for cytotoxicity analysis via alamarBlue (Life Technologies) reagent. The obtained solutions (12 mg ml−1) were further diluted into different concentrations (0.75, 1.5, 3.0 and 6.0 mg ml−1) and then incubated with fibroblast cells (CCD-112 CoN) at a density of 2.0 × 104 cells cm−2 in 96-well plates. The cell viability remained high at >95% after 48 h, demonstrating the negligible cytotoxicity of the side products of both films (Figure 6). Note that increasing concentrations of degradation products did not have any adverse impact on cell proliferation, and thus, the film is safe for use in biological applications.
Biocompatibility of the POSS-PCL-PU films was investigated by seeding CCD-112 CoN cells onto them and then incubating for 10 days before assessing the cell attachment and growth using confocal microscopy. After staining with fluorescein diacetate, the live fibroblast cells appeared as a green confluent monolayer on the slightly hydrophilic star-shaped POSS-PCL-PU film (Figures 7a and c). Optical sectioning along the z axis illustrates the growth at different focal planes and demonstrated that the fluorescein diacetate-stained green fibroblast cells were able to adhere and proliferate around and on the distinct spherulites features of the POSS-PCL-PU films (Figure 8 and Supplementary Figure S2). Imaris imaging software was used to further render three-dimensional visualization and cross-section views of the fibroblasts in the green channel (Figures 7d and e). The xz and yz view of the cross-sectional image demonstrated the growth of cells at different layers along the z axis. Another cell line, Mb231-GFP, was also observed to form a confluent layer on the star-shaped POSS-PCL-PU film (Supplementary Figure S3). However, it is the presence of POSS in PCL-PU that improves the cell-substrate affinity because the moderate hydrophilicity provides a good support for the cell adhesion,30 and the intrinsic roughness at sub-micrometer/nanometer scales enhances the contact guidance for cell interactions and growth.31 All these favorable factors (excellent biocompatibility, high porosity, moderate hydrophilicity and rough surface nanotopography) present in the star-shaped POSS-PCL-PU film created a very conducive environment for enhanced cell interactions, adhesion and proliferation. Future experiments will be focused on improving the bioactivity of the film for active interaction with the cellular components of the engineered tissues to facilitate and regulate their activities by including biological cues, such as cell-adhesive ligands, to enhance attachment or physical cues, such as topography, to influence cell morphology and alignment.
Conclusions
Star-shaped POSS-PCL-PU was synthesized and used to prepare highly porous films that exhibited controlled and slow initial degradation as biodegradable scaffold biomaterials for tissue engineering applications. For such applications, another important feature is the ability to control the degradation rate of the films to maintain their mechanical stability until the intended function is fulfilled before degrading into non-toxic byproducts. In addition, the film and its metabolic products exhibited excellent biocompatibility, with no adverse effects and hindrance on cell growth upon incubation with cells for 48 h. The inclusion of POSS in PCL-PU strengthens the cell-substrate affinity of the film by improving its hydrophilicity to provide a greater support for the cell adhesion and by increasing its intrinsic roughness at the sub-micrometer/nanometer level to enhance contact guidance for cell interactions and growth. The combination of organic and inorganic functionalities in the unique nanocomposite POSS-PCL-PU offers superior properties compared to traditional PCL-PU. In summary, the star-shaped POSS-PCL-PU film produced in this study, with its unique nanotopography, exhibits excellent biocompatibility, high porosity and controlled degradability, which favor cell adhesion and proliferation, thereby making it a great candidate as a scaffold biomaterial for use in tissue engineering.
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Acknowledgements
This study was supported by the Institute of Materials Research and Engineering (IMRE), the Agency of Science Technology and Research (A*STAR) in Singapore and the National University of Singapore. We thank Dr Yap Fung Ling from IMRE for her help with performing the AFM measurements and express our thanks to the Confocal Microscopy Unit, National University Health System.
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Teng, C., Mya, K., Win, K. et al. Star-shaped polyhedral oligomeric silsesquioxane-polycaprolactone-polyurethane as biomaterials for tissue engineering application. NPG Asia Mater 6, e142 (2014). https://doi.org/10.1038/am.2014.102
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DOI: https://doi.org/10.1038/am.2014.102
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