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Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus

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Abstract

A clue to the molecular mechanism of neoplastic transformation was provided by the finding of a near identity in amino-acid sequence between the platelet-derived growth factor (PDGF) B-chain and a region in the transforming protein, p28sis, of simian sarcoma virus (SSV)1–4, an agent that causes sarcomas and gliomas in experimental animals5,6. This finding infers a direct link between the molecular biology of normal mitogenesis and oncogenesis since it suggests that the transforming activity of SSV is caused by a growth factor. Although PDGF agonist activity has been isolated from conditioned medium of SSV-transformed cells7–10, it is not clear whether infection of responsive cells by SSV leads solely to autocrine stimulation of growth by a secreted PDGF-like factor or whether other, possibly intracellular, activities of p28sis or its processed products contribute to the transformation9,11,12. To distinguish between these possibilities, we have studied the effect of anti-PDGF antibodies on acute SSV-transformation, and report here that these antibodies inhibit both proliferation and SSV-induced morphological changes in human diploid fibroblasts.

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References

  1. Waterfield, M. D. et al. Nature 304, 35–39 (1983).

    Article  ADS  CAS  Google Scholar 

  2. Doolittle, R. F. et al. Science 221, 275–277 (1983).

    Article  ADS  CAS  Google Scholar 

  3. Johnsson, A. et al. EMBO J. 3, 921–928 (1984).

    Article  CAS  Google Scholar 

  4. Devare, S. G., Reddy, E. P., Law, J. D., Robbins, K. C. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 80, 731–735 (1983).

    Article  ADS  CAS  Google Scholar 

  5. Deinhardt, F. et al. J. med. Primatol. 1, 29–50 (1972).

    Article  CAS  Google Scholar 

  6. Johnson, L. Jr et al. Abstr. Proc. Am. Ass. Cancer Res. 16, 119 (1975).

    Google Scholar 

  7. Bowen-Pope, D. F., Vogel, A. & Ross, R. Proc. natn. Acad. Sci. U.S.A. 81, 2396–2400 (1984).

    Article  ADS  CAS  Google Scholar 

  8. Owen, A. J., Pantazis, P. & Antoniades, H. N. Science 225, 54–56 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Huang, J. S., Huang, S. S. & Deuel, T. F. Cell 39, 79–87 (1984).

    Article  CAS  Google Scholar 

  10. Johnsson, A., Betsholtz, C., Von der Helm, K., Heldin, C.-H. & Westermark, B. Proc. natn. Acad. Sci. U.S.A. 82, 1721–1725 (1985).

    Article  ADS  CAS  Google Scholar 

  11. Robbins, K. C., Antoniades, H. N., Devare, S. G., Hunkapiller, M. W. & Aaronson, S. A. Nature 305, 605–608 (1983).

    Article  ADS  CAS  Google Scholar 

  12. Deuel, T. F., Huang, J. S., Huang, S. S., Stroobant, P. & Waterfield, M. D. Science 221, 1348–1350 (1983).

    Article  ADS  CAS  Google Scholar 

  13. Ponten, J. Virol. Monogr. 8, 25–27 (1971).

    Google Scholar 

  14. Stoker, M. G. P. Nature 246, 200–203 (1973).

    Article  ADS  CAS  Google Scholar 

  15. Heldin, C.-H., Westermark, B. & Wasteson, Å. Expl Cell Res. 136, 255–261 (1981).

    Article  CAS  Google Scholar 

  16. Betsholtz, C. & Westermark, B. J. Cell. Physiol. 118, 203–210 (1984).

    Article  CAS  Google Scholar 

  17. Johnsson, A., Heldin, C.-H., Westermark, B. & Wasteson, Å. Biochem. biophys. Res. Commun. 104, 66–74 (1982).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medical and Physiological Chemistry, University of Uppsala, S-751 23, Uppsala, Sweden

    Ann Johnsson & Carl-Henrik Heldin

  2. Department of Pathology, University of Uppsala, S-751 23, Uppsala, Sweden

    Christer Betsholtz & Bengt Westermark

Authors
  1. Ann Johnsson
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  2. Christer Betsholtz
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  3. Carl-Henrik Heldin
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  4. Bengt Westermark
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Johnsson, A., Betsholtz, C., Heldin, CH. et al. Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus. Nature 317, 438–440 (1985). https://doi.org/10.1038/317438a0

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  • DOI: https://doi.org/10.1038/317438a0

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