Abstract
A major event of nervous system development1 is the migration of granule cell neurones, during the early postnatal development of the cerebellar cortex, from their germinating zone in the external granular layer to then final location in the internal granular layer. During migration, many granule cells are seen in direct cell-surface contact with processes of Bergmann glia, a subclass of astrocytes2. In the neurological mutant mouse weaver, however, migration of granule cells is impaired, probably due to a deficit in cell–cell interactions3–5. To gain insight into the cellular and molecular mechanisms involved in granule cell migration, we have used a modification of an in vitro assay system, previously described by Moonen et al.6, which displays migratory behaviour in small tissue explants during several days of suspension culture. The aim of this study was to investigate the process of granule cell migration by using antibodies directed against cell-surface components of developing neural cells. We report here that migration of 3H-thymidine-labelled granule cell neurones can be modified by Fab fragments of both mono- and polyclonal L1 antibodies, but not by Fab fragments of polyclonal antibodies prepared against mouse liver membranes, which also react with cerebellar cell surfaces.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cowan, W. M. Life Sci. Res. Rep. 24, 7–24 (1982).
Rakic, P. Life Sci. Res. Rep. 20, 25–38 (1982).
Rakic, P. & Sidman, R. L. J. comp. Neurol. 152, 103–132 (1973).
Rakic, P. & Sidman, R. L. J. comp. Neurol. 152, 133–162 (1973).
Sotelo, C. & Changeux, J.-P. Brain Res. 67, 519–526 (1974).
Moonen, G., Grau-Wagemans, M. P. & Selak, I. Nature 298, 753–755 (1982).
Rathjen, F. G. & Schachner, M. EMBO J. (in the press).
Miale, J. L. & Sidman, R. L. Expl Neurol. 4, 277–296 (1961).
Goridis, C., Joher, J. A., Hirsch, M. & Schachner, M. J. Neurochem. 31, 531–539 (1978).
Rohrer, H. & Schachner, M. Neurochemistry 35, 792–803 (1980).
Jorgensen, O. S., Delouvee, A., Thiery, J.-P. & Edelmann, G. M. FEBS Lett. 111, 39–42 (1980).
Hirn, M., Pierres, M., Deagostini-Bazin, H., Hirsch, M. & Goridis, C. Brain Res. 214, 433–439 (1981).
Brackenbury, R., Thiery, M.-P., Rutishauser, U. & Edelman, G. M. J. biol. Chem. 252, 6835–6840 (1977).
Hoffman, S. et al. J. biol. Chem. 257, 7720–7729 (1982).
Lemmon, V., Staros, E. B., Perry, H. E. & Gottlieb, D. I. Devl Brain Res. 3, 349–360 (1982).
Schnitzer, J. & Schachner, M. J. Immun. 1, 457–470 (1981).
Altman, J. J. comp. Neurol. 163, 427–448 (1975).
Caviness, V. S. & Rakic, P. A. Rev. Neurosci. 1, 297–326 (1978).
Fischer, G. Neurosci. Lett. 28, 325–329 (1982).
Goridis, C., Martin, J. & Schachner, M. Brain Res. Bull. 3, 45–52 (1978).
Willinger, M. & Schachner, M. Devl Biol. 74, 101–117 (1980).
Fujita, S. J. Cell Biol. 32, 277–287 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lindner, J., Rathjen, F. & Schachner, M. L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum. Nature 305, 427–430 (1983). https://doi.org/10.1038/305427a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/305427a0
- Springer Nature Limited
This article is cited by
-
The functional role of integrins during intra- and extravasation within the metastatic cascade
Molecular Cancer (2019)
-
Proteolytic cleavage of transmembrane cell adhesion molecule L1 by extracellular matrix molecule Reelin is important for mouse brain development
Scientific Reports (2017)
-
Small-molecule inhibitors of FGFR, integrins and FAK selectively decrease L1CAM-stimulated glioblastoma cell motility and proliferation
Cellular Oncology (2016)
-
Identification of novel mutations in L1CAM gene by a DHPLC-based assay
Genes & Genomics (2016)
-
Intercellular protein–protein interactions at synapses
Protein & Cell (2014)