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Biolistic Transfer of the Gene uidA and Its Expression in Haploid Embryo-like Structures of Triticale (×Triticosecale Wittmack)

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Abstract

Haploid embryo-like structures (ELS) of triticale were obtained by in vitro androgenesis. These structures were bombarded with gold microparticles 1 µm in diameter coated with the plasmid pAHGUS, using the Dupont PDS He/1000 apparatus. Analysis was made of the influence of genotype, duration of ELS pre-culture, helium pressure and shooting distance on the transfer and expression of the gene uidA. No significant differences were seen between genotypes or duration of pre-culture; differences were found, however, with respect to helium pressure and shooting distance. The combination of 1100 psi helium and a 6 cm shooting distance led to the greatest mean number of uidA expression foci in the ELS (1.63), and the greatest mean number of ELS showing at least one focus (0.38). With a pressure of 1800 psi and a 9 cm distance, only 0.35 foci were obtained per ELS, and only 0.18 ELS had one or more foci. These results are being used as references in a program for obtaining double haploid and transgenic triticale plants.

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  1. Departamento de Biología Celular y Genética, Facultad de Biología, Universidad de Alcalá, Campus Universitario, 28871, Alcalá de Henares, Madrid, Spain

    Silvia Rubio, Nicolás Jouve & Juan M. González

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  1. Silvia Rubio
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  2. Nicolás Jouve
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  3. Juan M. González
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Correspondence to Juan M. González.

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Rubio, S., Jouve, N. & González, J.M. Biolistic Transfer of the Gene uidA and Its Expression in Haploid Embryo-like Structures of Triticale (×Triticosecale Wittmack). Plant Cell, Tissue and Organ Culture 77, 203–209 (2004). https://doi.org/10.1023/B:TICU.0000016827.02241.1f

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