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Influence of Media Components and pH on Somatic Embryo Induction in Three Genotypes of Soybean

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Abstract

The influence of media components on the initiation of somatic embryogenesis in three genotypes of soybean was investigated. The following genotypes were used: Iroquois, Macon, and Savoy. Media modifications included sucrose concentration, type and concentration of auxin at two pH levels, and pH level independently. Immature cotyledons were used as the source of explant. Cotyledons were placed on a medium containing MS salts, B5 vitamins, sucrose, and auxin. Gelrite (0.2%) was used as the solidifying agent. Sucrose concentrations of 1, 2, 3, 4.5, or 6% were used. The auxins used included 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthaleneacetic acid (NAA) with each at concentrations of 45.2, 90.4, 135.7, 180.9, and 226.2 μM. The pH of each the media was adjusted to either 5.7 or 7.0 with 1 N NaOH. In an additional experiment, the effect of the two pH levels, 5.7 and 7.0, was investigated independently. Overall, the frequency of somatic embryogenesis significantly varied among the different genotypes used in this study, with Iroquois showing the highest response. Frequency of somatic embryogenesis also varied in response to the different treatments used, including sucrose and auxin. The highest initiation (91.7%) and mean number of somatic embryos per responding explant (14.9) of Iroquois was observed in a medium containing 2% sucrose. The highest initiation (97.1%) and mean number of somatic embryos per responding explant (19.5) was observed in Iroquois on 135.7 μM 2,4-D and Savoy on 135.7 μM 2,4-D, respectively, for the auxin by pH level experiment. No significant differences were observed among the two pH treatments used.

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Authors and Affiliations

  1. Department of Natural Resources and Environmental Sciences, University of Illinois, 1201 W. Gregory, Urbana, IL, 61801, USA

    Nicolle Hofmann & Schuyler S. Korban

  2. USDA-ARS and Department of Crop Science, University of Illinois, 1201 W. Gregory, Urbana, IL, 61801, USA

    Randall L. Nelson

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  1. Nicolle Hofmann
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  2. Randall L. Nelson
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  3. Schuyler S. Korban
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Correspondence to Schuyler S. Korban.

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Hofmann, N., Nelson, R.L. & Korban, S.S. Influence of Media Components and pH on Somatic Embryo Induction in Three Genotypes of Soybean. Plant Cell, Tissue and Organ Culture 77, 157–163 (2004). https://doi.org/10.1023/B:TICU.0000016819.74630.59

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  • DOI: https://doi.org/10.1023/B:TICU.0000016819.74630.59

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