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Distinct glycoforms of human α1-acid glycoprotein have comparable synthesis rates: a [13C]valine-labelling study in healthy humans

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Abstract

Various α1-acid glycoprotein (AGP) glycoforms are present in plasma differing in extent of branching and/or fucosylation of their 5 N-linked glycans, as well as in concentration. It is assumed that hepatic synthesis determines the relative occurrence of the AGP-glycoforms in plasma, but experimental evidence is lacking. In this study, we have investigated the contribution of fractional synthesis rates to the plasma concentration of AGP-glycoforms that differed in relative occurrence in healthy human plasma. During a [13C]valine infusion, AGP was isolated from the plasma of healthy volunteers. Four AGP-glycoforms, differing strongly in plasma concentration were obtained by sequential affinity chromatography over concanavalin-A- and Aleuria aurantia-agarose columns. The incorporation of the [13C]valine tracer into the AGP-glycoforms was measured by gas chromatography combustion isotope ratio mass spectrometry. The mean fractional synthesis rates of the four AGP-glycoforms did not differ significantly between each other as well between individuals. The results indicated a renewal of about 15%/day of the plasma pools of each of the AGP-glycoforms. This is in support to the assumption that the differences in plasma concentration of the AGP-glycoforms are a reflection of the state of the hepatic glycosylation process. Published in 2004.

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Authors and Affiliations

  1. Glycoimmunology Group, Department of Molecular Cell Biology & Immunology, VU Medical Center, Amsterdam, The Netherlands

    Dennis C.W. Poland & Willem van Dijk

  2. Metabolic Laboratory, Department of Clinical Chemistry, VU Medical Center, Amsterdam, The Netherlands

    Willem Kulik, Marcella M. Hallemeesch, Cornelis Jakobs & Kees de Meer

Authors
  1. Dennis C.W. Poland
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  2. Willem Kulik
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  3. Willem van Dijk
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  4. Marcella M. Hallemeesch
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  5. Cornelis Jakobs
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  6. Kees de Meer
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Correspondence to Willem van Dijk.

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Poland, D.C., Kulik, W., van Dijk, W. et al. Distinct glycoforms of human α1-acid glycoprotein have comparable synthesis rates: a [13C]valine-labelling study in healthy humans. Glycoconj J 20, 99–105 (2003). https://doi.org/10.1023/B:GLYC.0000018584.66712.2c

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  • DOI: https://doi.org/10.1023/B:GLYC.0000018584.66712.2c

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