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Effects of monocytic products, recombinant interleukin-1, and recombinant interleukin-6 on glycosylation of α1acid glycoprotein

Studies with primary human hepatocyte cultures and rats

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Abstract

Changes in the carbohydrate moieties of acute-phase glycoproteins (APGPs) often accompany the increase in their secretion by the liver during inflammation. In this study, we investigated whether factors known to regulate APGP gene expression are also involved in the altered glycosylation. For this purpose, the glycosylation pattern ofα 1,-acid glycoprotein (AGP) as secreted by human hepatocytes, cultured in the presence and absence of dexamethasone and monokines, was studied by crossed affino-(concanavalin A) immunoelectrophoresis (CAIE). The monokines rIL-1 and rIL-6, in the presence of dexamethasone, both stimulated AGP secretion and caused a change in glycosylation towards an increased Con A reactivity, including the appearance of two strongly reactive forms (D and E) normally not present. Dexamethasone alone did not influence either process. When tested in vivo in rats, rIL-6 also induced an increased presence of Con A-reactive forms of AGP in serum. In conclusion, the changes in secretion and glycosylation of AGP as seen during inflammation seem to be mediated by the same factor(s).

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References

  1. Kushner, I. 1982. The phenomenon of the acute-phase response.Ann. N. Y. Acad. Sci. 389:39.

    Google Scholar 

  2. Koj, A. 1985. Biological function of acute-phase proteins.In The Acute-Phase Response to Injury and Infection. A. H. Gordon and A. Koj, editors. Elsevier, Amsterdam. 139.

    Google Scholar 

  3. Nicollet, I., J. P. Lebreton, M. Fontaine, andM. Hiron. 1981. Evidence for alpha-1-acid glycoprotein populations of different pI values after concanavalin A affinity chromatography. Study of their evolution during inflammation in man.Biochim. Biophys. Acta 668:235.

    Google Scholar 

  4. Koj, A., A. Dubin, H. Kasperczak, J. Bereta, andA. H. Gordon. 1982. Changes in the blood level and affinity to concanavalin A of rat plasma glycoproteins during acute inflammation and hepatoma growth.Biochem. J. 206:545.

    Google Scholar 

  5. Mackiewicz, A., R. Marcinkowska-Pieta, S. Ballou, S. Mackiewicz, andI. Kushner. 1987. Microheterogeneity of alpha-1-acid glycoprotein in detection of intercurrent infection in systemic lupus erythematosus.Arthritis Rheum. 30:513.

    Google Scholar 

  6. Mackiewicz, A., T. Pawlowski, A. Mackiewicz-Pawlowska, K. Wiktorowicz, andS. Mackiewicz. 1987. Microheterogeneous forms of alpha-1-acid glycoprotein as indicators of rheumatoid arthritis activity.Clin. Chim. Acta 163:185.

    Google Scholar 

  7. Bayard, B., andJ.-P. Kerckaert. 1980. Evidence for the carbohydrate chains in individual glycoprotein molecular variants.Biophys. Biochem. Res. Commun. 95:777.

    Google Scholar 

  8. Wells, C., T. C. Bøg-Hansen, E. H. Cooper, andM. R. Class. 1981. The use of Concanavalin A crossed associated variations in alpha-1-acid glycoprotein.Clin. Chim. Acta 109:59.

    Google Scholar 

  9. Van Gool, J., W. Boers, M. Sala andN. C. J. J. Ladiges. 1984. Glucocorticoids and catecholamins as mediators of acute-phase proteins, especially ratα-macrofoetoprotein.Biochem. J. 220:125.

    Google Scholar 

  10. Baumann, H., C. Richards, andJ. Gauldie. 1987. Interaction among hepatocyte-stimulating factors, interleukin-1 and glucocorticoids for regulation of acute phase plasma proteins in human hepatoma (HepG2) cells.J. Immunol. 139:4122.

    Google Scholar 

  11. Darlington, G. J., D. R. Wilson, andL. Lachman. 1986. Monocyte-conditioned medium, interleukin-1 and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro.J. Cell. Biol. 103:787.

    Google Scholar 

  12. Dinarello, C. A. 1984. Interleukin-1.Rev. Infect. Dis. 6:51.

    Google Scholar 

  13. Baumann, H., C. L. Firestone, T. L. Burgess, K. W. Gross, K. R. Yamamoto, andW. A. Held. 1983. Dexamethasone regulation ofα 1-acid glycoprotein and other acute-phase reactants in rat liver and hepatoma cells.J. Biol. Chem. 258:563.

    Google Scholar 

  14. Baumann, H., G. P. Jahreis, D. N. Sauder, andA. Koj. 1984. Human keratinocytes and monocytes release factors which regulate the synthesis of major acute phase plasma proteins in hepatic cells from man, rat and mouse.J. Biol. Chem. 259:7331.

    Google Scholar 

  15. Koj, A., J. Gauldie, E. Regoeczi, D. N. Sauder, andG. D. Sweeney. 1984. The acutephase response of cultured rat hepatocytes: System characterization and the effect of human cytokines.Biochem. J. 224:505.

    Google Scholar 

  16. Aarden, L. A., E. De Groot, O. L. Schaap, andP. M. Lansdorp. 1987. Production of hybridoma growth factor by human monocytes.Eur. J. Immunol. 17:1411.

    Google Scholar 

  17. Brakenhoff, J. P. J., E. De Groot, R. F. Evers, H. Pannekoek, andL. A. Aarden. 1987. Molecular cloning and expression of hybridoma growth factor in Escherichia coli.J. Immunol. 139:4116.

    Google Scholar 

  18. Gauldie, J., C. Richards, D. Harnish, P. Lansdorp, andH. Baumann. 1987. Interferonβ2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells.Proc. Natl. Acad. Sci. U.S.A. 84:7251.

    Google Scholar 

  19. Haegeman, J. C., J. Content, G. Volckaert, R. Derijnck, J. Tavernier, andW. Fiers. 1986. Structural analysis of the sequence coding for an inducible 26-kDa protein in human fibroblasts.Eur. J. Biochem. 159:625.

    Google Scholar 

  20. Zilberstein, A., R. Ruggeri, J. H. Korn, andW. Revel. 1986. Structure and expression of cDNA and genes for human interferon-beta-2, a distinct species, inducible by growth stimulatory cytokines.EMBO J. 5:2529.

    Google Scholar 

  21. Hirano, T., K. Yasukawa, H. Harada, T. Tage, W. Yasuo, T. Matsuda, S. Kishiwamure, K. Nakajima, K. Koyama, A. Iwamatsu, S. Tsunasawa, F. Sakiyama, H. Matsui, Y. Takahara, T. Taniguchi, andT. Kishimoto. 1986. Complementary DNA for a novel human interleukin (BSF-2) that induces B-lymphocytes to produce immunoglobulin.Nature 324:73.

    Google Scholar 

  22. Poupart, P., P. Vanden Beele, S. Gayphas, J. Van Snick, G. Haegeman, V. Kruys, W. Fiers, andJ. Content. 1987. B cell growth differentiating activity of recombinant human 26kD protein (BSF-2, HuIFN-2, HPGF).EMBO J. 6:1219.

    Google Scholar 

  23. Le, J., andJ. Vilcek. 1987. Tumor necrosis factor and interleukin 1: Cytokines with multiple overlapping biological activities.Lab. Invest. 56:234.

    Google Scholar 

  24. Baumann, H., V. Onorato, J. Gauldie, andG. P. Jahreis. 1987. Distinct sets of acutephase plasma proteins are stimulated by separate human hepatocyte-stimulating factors and monokines in rat hepatoma cells.J. Biol. Chem. 262:9756.

    Google Scholar 

  25. Baumann, H., R. E. Hill, D. N. Sauder andG. P. Jahreis. 1986. Regulation of major acute-phase plasma proteins by hepatocyte-stimulating factors of human squamous carcinoma cells.J. Cell. Biol. 102:370.

    Google Scholar 

  26. Gauldie, J., D. N. Sauder, K. P. W. J. McAdam, andC. A. Dinarello. 1987. Purified interleukin-1 (II-1) from human monocytes stimulates acute-phase protein synthesis by rodent hepatocytes in vitro.Immunology 60:203.

    Google Scholar 

  27. Koj, A., A. Kurdowska, D. Magielska-Zero, H. Rokita, J. D. Sipe, J. M. Dayer, andS. Demczu. 1987. Limited effects of recombinant human and murine II-1 and TNF on the production of acute-phase proteins by cultured rat hepatocytes.Biochem. Int. 14:553.

    Google Scholar 

  28. Helle, M., J. P. J. Brakenhoff, E. R. De Groot, andL. A. Aarden. 1988. Interleukin-6 is involved in interleukin-1-induced activities.Eur. J. Immunol. 18:957.

    Google Scholar 

  29. Pos, O., W. van Dijk, N. C. J. J. Ladiges, C. Linthorst, M. Sala, D. van Tiel, andW. Boers. 1988. Glycosylation of four acute-phase glycoproteins secreted by rat liver cells in vivo and in vitro: Effects of inflammation and dexamethasone.Eur. J. Cell. Biol. 46:121.

    Google Scholar 

  30. Moshage., H. J., J. A. M. Janssen, J. H. Franssen, J. C. M. Hafkenscheid, andS. H. Yap. 1987. Study on the molecular mechanism of the decreased liver synthesis of albumin in inflammation.J. Clin. Invest. 79:1635.

    Google Scholar 

  31. Mackiewicz, A., M. K. Ganapahti, D. Schultz, andI. Kushner. 1987. Monokines regulate glycosylation of acute-phase proteins.J. Exp. Med. 166:253.

    Google Scholar 

  32. Bierhuizen, M. F. A., M. De Wit, C. A. R. L. Govers, W. Ferwerda, C. Koeleman, O. Pos, andW. Van Dijk. 1988. Glycosylation of three molecular forms of human α1-acid glycoprotein having different interactions with concanavalin A: Variations in the occurrence of bi-, tri and tetraantennary glycans and the degree of sialylation.Eur. J. Biochem. 175:387.

    Google Scholar 

  33. Ritchie, D. G., andG. M. Fuller. 1981. An in vitro bioassay for leukocytic endogeneous mediators using cultured rat hepatocytes.Inflammation 5:275.

    Google Scholar 

  34. Moshage, H. J., P. J. M.Rijntjes, J. C. M.Hafkenscheid, H. M. J.Roelofs, and S. H.Yap. 1988. Primary culture of human hepatocytes on homologous extracellular matrix: Influence of monocytic products on albumin synthesis.J. Hepatol. (in press).

  35. Bøg-Hansen, T. C., P. Prahl, andM. Lovenstein. 1978. Set of analytical electrophoresis experiments to predict results of affinity Chromatographic and separations. Fractionation of allergens from cows hair and human dander.J. Immunol. Methods 22:293.

    Google Scholar 

  36. Raynes, I. 1982. Variations in the relative proportions of microheterogeneous forms of plasma glycoproteins in pregnancy and disease.Biomedicine (Paris) 36:77.

    Google Scholar 

  37. Rijntjes, P. J. M., H. J. Moshage, P. J. L. Van Gemert, R. De Waal, andS. H. Yap. 1986. Cryopreservation of adult human hepatocytes.J. Hepatol. 3:7.

    Google Scholar 

  38. Gery, I., R. K. Gershon, andB. H. Waksman. 1972. Potentiation of the T lymphocyte response to mitogens. I the responding cell.J. Exp. Med. 136:128.

    Google Scholar 

  39. Baumann, H., andL. E. Maquat. 1986. Localization of DNA sequences involved in dexamethasone-dependent expression of the ratα 1,-acid glycoprotein gene.Mol. Cell. Biol. 6:2551.

    Google Scholar 

  40. Prowse, K. R. andH. Baumann. 1988. Hepatocyte-factor,β2-interferon, and interleukin-1 enhance expression of ratα 1-acid glycoprotein gene via a distal upstream regulatory region.Mol. Cell. Biol. 8:42.

    Google Scholar 

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Authors and Affiliations

  1. Department of Medical Chemistry, Faculty of Medicine, Free University, P.O. Box 7161, 1007, MC, Amsterdam

    Odette Pos, Jeske P. M. Snieders & Willem Van Dijk

  2. Division of Gastrointestinal and Liver Diseases, Department of Medicine, St. Radboud University Hospital, Nijmegen

    Han J. Moshage & Sing H. Yap

  3. Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, Amsterdam

    Lucien A. Aarden

  4. Laboratory of Experimental Medicine, Academic Medical Centre, University of Amsterdam, Amsterdam

    Jacobus Van Gool, Willem Boers & Astrid M. Brugman

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  1. Odette Pos
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  2. Han J. Moshage
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Pos, O., Moshage, H.J., Yap, S.H. et al. Effects of monocytic products, recombinant interleukin-1, and recombinant interleukin-6 on glycosylation of α1acid glycoprotein. Inflammation 13, 415–427 (1989). https://doi.org/10.1007/BF00914925

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