Abstract
Humanα 1-acid glycoprotein (AGP) has been shown to modulate various cellular and humoral immune reactions in vitro. Using glycosidase-modified derivatives of AGP, the importance of its carbohydrate moiety with regard to these effects has been noted. In normal serum, three molecular AGP forms interacting differently with concanavalin A (Con A) are present. The ratio of these forms is often changed during various physiopathological conditions. In this study, we could show that differences exist between the three AGP forms with regard to their immunomodulatory effectiveness. At physiological concentrations, the Con A-nonreactive variant AGP-A induced a stronger inhibition of the anti-CD3 stimulated lymphocyte proliferation than the other forms. Interestingly, AGP-A was also found to be responsible for the stimulation of lymphocyte proliferation induced by low AGP concentrations in vitro. Both immunomodulatory effects of AGP were abrogated by desialylation of the glycoprotein. These results support an immunomodulatory role of AGP in conditions characterized by a changed ratio of the differently glycosylated AGP forms.
Similar content being viewed by others
References
Kushner, I. 1982.Ann. N.Y. Acad. Sci. 389:39.
Van Oss C. J., C. F. Gillman, P. M. Bronson, andJ. R. Border. 1974. Phagocytosis inhibiting properties of human serum alpha-1-acid glycoprotein.Immunol. Commun. 3:321.
Chiu, K. M., R. F. Mortensen, A. P. Osmand, andH. Gewurz. 1977.Immunology 32:997–1005.
Bennett, M., andK. Schmid. 1980.Proc. Natl. Acad. Sci. U.S.A. 77:6109–6113.
Cheresh, D. A., J. A. Distasio, C. L. Vogel, andD. M. Lopez. 1982.J. Natl. Cancer Inst. 68:779.
Cheresh, D. A., D. H. Haynes, andJ. A. Distasio. 1984.Immunology 51:541–548.
Singh, V. K., andH. H. Fudenberg. 1986/1987.Immunol. Lett. 14:9–13.
Durand, G. 1989.Progress Clin. Biol. Res. 300:247–265.
Singh, V. K. 1989.Progress Clin. Biol. Res. 300:283–286.
Schmid, K. 1975.α-Acid glycoproteinIn The Plasma Proteins. F. W. Ritman, editor. Academic Press, New York. 183–226.
Schmid, K., R. B. Nimberg, A. Kimura, H. Yamaguchi, andJ. P. Binette. 1977. B.B.A.492:291–302.
Bayard, B., andJ.-P. Kerckaert. 1980.Biochem. Biophys. Res. Commun. 95:777–784.
Wells, C., T. C. Bog-Hansen, E. H. Cooper, andM. R. Class. 1981.Clin. Chim. Acta 109:59–67.
Nicollet, L., J.-P. Lebreton, M. Fontaine, andM. Hiron. 1981.Biochim. Biophys. Acta 668:235–245.
Bierhuizen, M. F. A., M. de Wit, C. A. R. L. Govers, W. Ferwerda, C. Koeleman, O. Pos, andW. van Dijk. 1988.Eur. J. Biochem. 175:387–394.
Mackiewicz, A., R. Marcinkowska-Pieta, S. Ballou, S. Mackiewicz, andI. Kushner. 1987.Arthritis Rheum. 30:513.
Raynes, J. 1982Biomedicine (Paris) 36:77.
Hao, Y.-L., andM. Wickerhauser. 1973.Biochim. Biophys. Acta 322:99–108.
Tax, W., H. W. Willems, P. P. M. Reekers, P. J. A. Capel, andR. A. P. Koene. 1983.Nature 304:445.
Bog-Hansen, T. C., P. Prahl, andM. Lovenstein. 1978.J. Immunol. Methods 22:293.
Boyum, A. 1968.Scand. J. Clin. Lab. Invest. 21(Suppl. 97):77.
Pos, O., M. E.van der Stelt, G.-J.Wolbink, M.Nusten, and W.van Dijk. 1989. (submitted).
Gahmberg, C. G., andL. C. Andersson. 1978.J. Exp. Med. 148:507.
Le Jeune, P.-J., B. Mallet, C. Farnarier, andS. Kaplanski. 1989.Biochim. Biophys. Acta 990:122.
Schmid, K., H. Kaufmann, S. Isemura, F. Bauer, J. Emura, T. Motoyama, M. Ishiguro, andS. Nanno. 1973.Biochemistry 12:2711.
Van Wauwe, J. P., J. R. de Mey, andJ. G. Goossens. 1980.J. Immunol. 124:2708.
Bradford, M. M. 1976.Anal. Biochem. 72:428.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pos, O., Oostendorp, R.A.J., Van Der Stelt, M.E. et al. Con A-nonreactive human α1-acid glycoprotein (AGP) is more effective in modulation of lymphocyte proliferation than Con A-reactive AGP serum variants. Inflammation 14, 133–141 (1990). https://doi.org/10.1007/BF00917452
Issue Date:
DOI: https://doi.org/10.1007/BF00917452