Abstract
CHO-K1 and VERO cells have been grown on MicroHex™, a polystyrene-based microsupport with two-dimensional geometry and the consistency of their growth kinetics were established. These cells have been detached by exposure to buffered ethylenediaminetetraacetic acid (EDTA) followed by controlled mechanical shear to yield well-isolated cells suspended in EDTA. Neutralisation of the EDTA followed by restoration of the chemical composition of the growth medium causes detached CHO-K1 cells to display unaltered growth kinetics.
Similar content being viewed by others
References
Bio Directory (2002) Amersham Biosciences, pp. 53–54.
Chowdhury JR, Chowdhury NR, Demetrious AA & Wilson JM (1989) Use of microbeads for cell transplantation. In: Miller AOA (ed.) Advanced Research in Animal Cell Technology (pp. 315–317) Kluwer Academic Publishers, Dordrecht.
Jones G (1991) Measurement of Cell Adhesion in Suspension. In: Curtis ASG & Lackie JM (eds) Measuring Cell Adhesion (pp. 23–39) John Wiley & Sons, New york.
Kenda-Ropson N, Mention D, Motte V, Genlain M & Miller AOA (2002a) Microsupport with two-dimensional geometry (2D-MS). 3. In situ determination of the growth kinetics of anchorage-dependent cells by laser diffraction particle sizing (LDPS). Cytotechnology 37: 49–53.
Kenda-Ropson N, Lenglois S & Miller AOA (2002b) Microsupport with two-dimensional geometry (2D-MS). 4. Temperature-induced detachment of anchorage-dependent CHO-K1 cells from cryoresponsive MicroHexTM (CryoHex). Cytotechnology 39: 163–170.
Merten O-W, Dante J, Noguiez-Hellin P, Laune S, Klatzmann D, Salzmann J-L (1997) New process for cell detachment: use of heparin. In: Corrondo MJT, Griffiths JB Moreira JLP (eds) Animal cell Technology: From Vaccines to Genetic Medecine (pp. 343–348) Kluwer Academic Press, Dordrecht.
Merten O-W (1999) Cell detachment. In: Spier RE (ed.) The Encyclopedia of Cell Technology (pp. 351–365) John Wiley & Sons, New York.
Miller AOA, Menozzi FD & Dubois D (1989) Microbeads and anchorage-dependent eukaryotic cells: the beginning of a new era in biotechnology. In: Fiechter A (ed.) Advances in Biochemical Engineering/Biotechnology, Vol. 39 (pp. 73–95) Springer Verlag, Berlin.
Van Wezel AL (1967) Growth of cell-strains and primary cells on microcarriers in homogeneous culture. Nature 216: 64–65.
Rights and permissions
About this article
Cite this article
Lenglois, S., Moser, M. & Miller, A. Microsupport with Two-Dimensional Geometry (2D-MS). Cytotechnology 44, 47–54 (2004). https://doi.org/10.1023/B:CYTO.0000043403.20008.02
Issue Date:
DOI: https://doi.org/10.1023/B:CYTO.0000043403.20008.02