Abstract
Creation of well-controlled two dimensional cell culture systems continues to be important for the advancement of basic cell biology investigations as well as innovative platforms for drug development, toxicity testing, and diagnostic assays. These cell culture systems have become more advanced in order to provide the appropriate biomechanical and biochemical cues that better mimic the milieu of in vivo conditions. Additionally the platforms themselves can aid in the measurement of cell/cell and cell/substrate mechanical interactions through the use of microscopy techniques. Here we review state-of-the art cell culture systems that involve a range of different substrates, protein micropatterning, and markers for measurement of strains and tractions, including those developed by our labs. We will evaluate the advantages and disadvantages of several culture systems with a focus on those used with stem cell-derived cell types and provide examples of the quantitative measures that can be made with techniques such as traction force microscopy, digital image correlation, and scanning gradient Fourier transform analysis.
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References
Zhang, B.Y., Xiao, Y., Hsieh, A., Thavandiran, N., Radisic, M.: Micro- and nanotechnology in cardiovascular tissue engineering. Nanotechnology. 22, 494003 (2011)
Almany, L., Seliktar, D.: Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures. Biomaterials. 26, 2467–2477 (2005)
Ralphe, J.C., de Lange, W.J.: 3D engineered cardiac tissue models of human heart disease: learning more from our mice. Trends Cardiovasc. Med. 23, 27–32 (2013)
Bray, M.A., Sheehy, S.P., Parker, K.K.: Sarcomere alignment is regulated by myocyte shape. Cell Motil. Cytoskeleton. 65, 641–651 (2008)
Ribeiro, A.J., Ang, Y.S., Fu, J.D., Rivas, R.N., Mohamed, T.M., Higgs, G.C., et al.: Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness. Proc. Natl. Acad. Sci. U. S. A. 112, 12705–12710 (2015)
Discher, D.E., Mooney, D.J., Zandstra, P.W.: Growth factors, matrices, and forces combine and control stem cells. Science. 324, 1673–1677 (2009)
Salick, M.R., Napiwocki, B.N., Sha, J., Knight, G.T., Chindhy, S.A., Kamp, T.J., et al.: Micropattern width dependent sarcomere development in human ESC-derived cardiomyocytes. Biomaterials. 35, 4454–4464 (2014)
Napiwocki, B.N., Salick, M.R., Ashton, R.S., Crone, W.C.: Controlling hESC-CM cell morphology on patterned substrates over a range of stiffness. In: Mechanics of Biological Systems and Materials, vol. 6, pp. 161–168. Springer, Cham (2017)
Napiwocki, B.N., Salick, M.R., Ashton, R.S., Crone, W.C.: Polydimethylsiloxane lanes enhance sarcomere Organization in Human ESC-derived Cardiomyocytes. In: Tekalur, S.A., Zavattieri, P., Korach, C.S. (eds.) Mechanics of Biological Systems and Materials, vol. 6, pp. 105–111. Springer, New York (2016)
Notbohm, J., Banerjee, S., Utuje, K.J., Gweon, B., Jang, H., Park, Y., et al.: Cellular contraction and polarization drive collective cellular motion. Biophys. J. 110, 2729–2738 (2016)
Knight, G., Sha, J., Ashton, R.: Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology. Chem. Commun. 51, 5238–5241 (2015)
Dembo, M., Wang, Y.L.: Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys. J. 76, 2307–2316 (1999)
Butler, J.P., Tolic-Norrelykke, I.M., Fabry, B., Fredberg, J.J.: Traction fields, moments, and strain energy that cells exert on their surroundings. Am. J. Phys. Cell Phys. 282, C595–C605 (2002)
Salick, M.R., Napiwocki, B.N., Knight, G.T., Ashton, R.S., Crone, W.C.: The scanning gradient Fourier transform (SGFT) method for assessing sarcomere organization, alignment, and heterogeneity, In preparation
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Napiwocki, B.N., Stempien, A., Notbohm, J., Ashton, R.S., Crone, W. (2018). Two-Dimensional Culture Systems to Investigate Mechanical Interactions of the Cell. In: Zavattieri, P., Minary, M., Grady, M., Dannemann, K., Crone, W. (eds) Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics, Volume 4. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-319-63552-1_6
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DOI: https://doi.org/10.1007/978-3-319-63552-1_6
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