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Effects of Photoperiod and Plant Developmental Stage on NADH Dehydrogenase and Photosystem Activities of Isolated Chloroplasts

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Biologia Plantarum

Abstract

Barley (Hordeum vulgare L. cv. Hassan) leaves were used to study the effects of developmental stage and photoperiod on the NAD(P)H-ferricyanide oxidoreductase (NAD(P)H-FeCNR) and on the photosystem (PS) 1 and 2 activities of isolated chloroplasts. From day 6 to day 12, both the PS 1 and NADH-FeCNR activities decreased while NADPH-FeCNR activity remained almost unchanged. Methyl jasmonate had no significant effect on the NAD(P)H-FeCNR activity changes. In 6- to 7- and 14- to 15-d-old plants, the NADH-FeCNR activity was higher during the photoperiod than during the dark period and, in the 14- to 15-d-old plants, the PS 1 activity increased during photoperiod in the same way, but to a lesser extent. The PS 1 activity of plants during a dark-accelerated senescence was low. The simultaneous changes in chloroplast PS 1 and NADH-FeCNR activities support the role proposed for the chloroplast NADH dehydrogenase complex in the cyclic electron transport.

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Authors and Affiliations

  1. Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, E-30100, Murcia, Spain e-mail

    J. Cuello & M.J. Quiles

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  1. J. Cuello
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  2. M.J. Quiles
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Correspondence to J. Cuello.

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Cuello, J., Quiles, M. Effects of Photoperiod and Plant Developmental Stage on NADH Dehydrogenase and Photosystem Activities of Isolated Chloroplasts. Biologia Plantarum 43, 393–398 (2000). https://doi.org/10.1023/A:1026790227572

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