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Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B

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Abstract

The reduced content of photoreceptors, such as phytochromes, can decrease the efficiency of photosynthesis and activity of the photosystem II (PSII). For the confirmation of this hypothesis, the effect of deficiency in both phytochromes (Phy) A and B (double mutant, DM) in 7–27-day-old Arabidopsis thaliana plants on the photosynthetic activity was studied in absence and presence of UV-A radiation as a stress factor. The DM with reduced content of apoproteins of PhyA and PhyB and wild type (WT) plants with were grown in white and red light (WL and RL, respectively) of high (130 μmol quanta m−2 s−1) and low (40 μmol quanta m−2 s−1) intensity. For DM and WT grown in WL, no notable difference in the photochemical activity of PSII was observed. However, the resistance of the photosynthetic apparatus (PA) to UV-A and the rate of photosynthesis under light saturation were lower in the DM compared to those in the WT. Growth in RL, when the photoreceptors of blue light—cryptochromes—are inactive, resulted in the significant decrease of the photochemical activity of PSII in DM compared to that in WT including amounts of QB-non-reducing complexes of PSII and noticeable enhancement of thermal dissipation of absorbed light energy. In addition, marked distortion of the thylakoid membrane structure was observed for DM grown in RL. It is suggested that not only PhyA and PhyB but also cryptochromes are necessary for normal functioning of the PA and formation of the mechanisms of its resistance to UV-radiation.

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Abbreviations

Chl:

Chlorophyll

Phy:

Phytochrome

RL:

Red light

FRL:

Far-red light

PSII:

Photosystem II

PA:

Photosynthetic apparatus

WT:

Wild type

DM:

phytochrome A, phytochrome B mutant

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Acknowledgments

This work was supported by grants from the Russian Foundation for Basic Research (Nos: 15-04-01199 and 14-04-01549), and by the Molecular and Cell Biology Programs from the Russian Academy of Sciences. The authors acknowledge support by the German Research Foundation DFG (cluster of excellence “Unifying Concepts in Catalysis”) and the COST MP1205 framework.

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, Moscow Region, 142290, Russia

    Vladimir D. Kreslavski, Anatoly A. Kosobryukhov, Galina N. Shirshikova, Aleksandra Yu Khudyakova & Suleyman I. Allakhverdiev

  2. Controlled Photobiosynthesis Laboratory, Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia

    Vladimir D. Kreslavski, Aleksandra Yu Khudyakova & Suleyman I. Allakhverdiev

  3. Technical University of Berlin, Institute of Chemistry, Max-Volmer-Laboratory of Biophysical Chemistry, Straße des 17. Juni 135, 10623, Berlin, Germany

    Franz-Josef Schmitt

  4. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pushchino, Moscow Region, 142290, Russia

    Galina A. Semenova

  5. Department of Plant Physiology, Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, Moscow, 119991, Russia

    Suleyman I. Allakhverdiev

  6. Bionanotechnology Laboratory, Institute of Molecular Biology and Biotechnology, Azerbaijan National Academy of Sciences, Matbuat Avenue 2a, Baku, 1073, Azerbaijan

    Suleyman I. Allakhverdiev

Authors
  1. Vladimir D. Kreslavski
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  2. Anatoly A. Kosobryukhov
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  3. Franz-Josef Schmitt
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  4. Galina A. Semenova
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  5. Galina N. Shirshikova
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  6. Aleksandra Yu Khudyakova
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  7. Suleyman I. Allakhverdiev
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Correspondence to Vladimir D. Kreslavski or Suleyman I. Allakhverdiev.

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Kreslavski, V.D., Kosobryukhov, A.A., Schmitt, FJ. et al. Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B. Protoplasma 254, 1283–1293 (2017). https://doi.org/10.1007/s00709-016-1020-9

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