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Paracrine effects of a uterine agglutinin are mediated via the sialic acids present in the rat uterine endometrium

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Abstract

A 32 kDa estrogen-induced, sialic acid-specific agglutinin (P-SAS) was isolated from rat endometrium in its proestrus stage [1]. To investigate the functional importance of P-SAS in the uterine milieu, specific binding assays were carried out with 125I-labeled P-SAS and different cellular components of the uterus (epithelial, stromal and myometrial cells), that were isolated from different stages of the estrus cycle. The results indicate that although the protein is secreted from the epithelial cells in the estrogenic phase, it binds specifically to the stromal cells, especially to those isolated from the diestrus stage of the estrus cycle. The specific binding, however, is seen to decrease with the progression of pregnancy. Scatchard analysis performed with varying amounts of 125I-P-SAS in the presence of excess cold P-SAS revealed that the binding occurs with a Ka = 1.69 × 108 M-1. As P-SAS binds specifically to sialic acids on the stromal cell surface, further characterization of the sialic acid molecule to which P-SAS binds was carried out by gas liquid chromatography (GLC). The studies revealed that P-SAS preferentially binds to N-glycolylneuraminic acid, which is attached to the penultimate sugar of the stromal cell surface glycoprotein chain via α2,6 linkage. As P-SAS is further known to be mitogenic [2], the effect of P-SAS on cultured stromal cells was studied in vitro. The growth regulatory assays revealed that P-SAS induced 3H-thymidine uptake by stromal cells in culture. Thus, from the above observations, paracrine effects of P-SAS on the stromal cells and on the subsequent growth and development of the uterus can be assumed.

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References

  1. Chowdhury M, Sarkar M, Mandal C: Identification and isolation of an agglutinin from uterus of rats. Biochem Biophys Res Commun 130: 1301–1307, 1985

    Google Scholar 

  2. Bhattacharyya I, Mandal C, Chowdhury M: Functional heterogeneity of sialic acid binding agglutinins of rat uteri towards in vitro lymphocyte transformation. Am J Reprod Immunol 20: 81–86, 1989

    Google Scholar 

  3. Ivatt RJ: Role of glycoproteins during early mammalian embryogenesis. In: The Biology of Glycoproteins. Plenum Press, New York, 1984, pp 95–181

    Google Scholar 

  4. Boströ m H, Odelblad E: Autoradiographic observations on the uptake of S35 in the genital organs of the female rat and rabbit after injection of labelled sodium sulfate. Acta Endocrinol Copenhagen 10: 89–96, 1952

    Google Scholar 

  5. Walter I, Bavdek S: Lectin binding patterns of porcine oviduct mucosa and endometrium during the oestrous cycle. J Anat 190: 299–307, 1997

    Google Scholar 

  6. Dutt A, Tang JP, Carson DD: Estrogen preferentially stimulates lactosaminoglycan-containing oligosaccharide synthesis in mouse uteri. J Biol Chem 263: 2270–2279, 1988

    Google Scholar 

  7. Dutt A, Tang JP, Welply JK, Carson DD: Regulation of N-linked glycoprotein assembly in uteri by steroid hormones. Endocrinology 118: 661–673, 1986

    Google Scholar 

  8. Varki A: Sialic acids as ligands in recognition phenomena. FASEB J 11: 248–255, 1997

    Google Scholar 

  9. Kelm S, Schauer R: Sialic acids in molecular and cellular interactions. Int Rev Cytolog 175: 137–240, 1997

    Google Scholar 

  10. Jones CJP, Kimber SJ, Illingworth I, Aplin JD: Decidual sialylation showed species-specific differences in the pregnant mouse and rat. J Reprod Fertil 106: 241–250, 1996

    Google Scholar 

  11. Weitlauf HM: The biology of implantation. In: E. Knobil, J.D. Neill (eds). The Physiology of Reproduction. Raven Press, New York, 1994, pp 391–440

    Google Scholar 

  12. Tang B, Guller S, Gurpide E: Mechanism of human endometrial stromal cell decidualization. Ann NY Acad Sci 734: 19–25, 1994

    Google Scholar 

  13. Kimber SJ, Illingworth I, Glasser SR: Expression of carbohydrate antigens in the rat uterus during early pregnancy and after ovariectomy and steroid replacement. J Reprod Fertil 103: 75–87, 1995

    Google Scholar 

  14. Jones CJP, Aplin JD, Mulholland J, Glasser SR: Patterns of sialylation in differentiating rat decidual cells as revealed by lectin histochemistry. J Reprod Fertil 99: 635–645, 1993

    Google Scholar 

  15. Zhu ZM, Kojima N, Stroud MR, Hakomori SI, Fenderson BA: Monoclonal antibody directed to Ley oligosaccharide inhibits implantation in the mouse. Biol Reprod 52: 903–912, 1995

    Google Scholar 

  16. Nelson JD, Jato-Rodriguez JJ, Mookerjea S: Effect of ovarian hormones on glycosyltransferase activities in the endometrium of ovariectomized rats. Arch Biochem Biophys 169: 181–191, 1975

    Google Scholar 

  17. Banerjee M, Chowdhury M: Purification and characterization of a sperm-binding glycoprotein from human endometrium. Human Reprod 9: 1497–1504, 1994

    Google Scholar 

  18. Chakraborty I, Chowdhury M: Activation of epididymal sperm by sialic acid-binding (SAS) agglutinin of uterus in rats. Mol Androl 5: 59–68, 1993

    Google Scholar 

  19. Banerjee M, Chowdhury M: Induction of capacitation in human spermatozoa in vitro by an endometrial sialic acid-binding lectin. Human Reprod 10: 3147–3153, 1995

    Google Scholar 

  20. Chakraborty I, Mandal C, Chowdhury M: Modulation of sialic acidbinding proteins of rat uterus in response to changing hormonal milieu. Mol Cell Biochem 126: 7–86, 1993

    Google Scholar 

  21. Kohn J, Wilchek M: A new approach (cyanotransfer) for cyanogen bromide activation of sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives. Biochem Biophys Res Commun 107: 878–884, 1982

    Google Scholar 

  22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275, 1951

    Google Scholar 

  23. McCormack SA, Glasser SR: Differential response of individual cell types from immature rats treated with estradiol. Endocrinol 106: 1634–1649, 1980

    Google Scholar 

  24. Satyaswaroop PG, Bressler RS, de la Pena MM, Gurpide E: Isolation and culture of human endometrial glands. J Clin Endocrinol Metab 48: 639–641, 1979

    Google Scholar 

  25. Rajabi MR, Singh A: Cell origin and paracrine control of intestinal collagenase in the Guinea pig uterine cervix–Evidence for a low molecular weight epithelial cell-derived collagenase stimulator Biol Reprod 52: 516–523, 1995

    Google Scholar 

  26. Scatchard G: The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51: 650–672, 1949

    Google Scholar 

  27. Stockert RJ, Morell AG, Scheinberg IH: Mammalian hepatic lectin. Science 186: 365–366, 1974

    Google Scholar 

  28. Veh RW, Michelski J-C, Corfield AP, Sander M, Gies D, Schauer R: A new chromatographic system for the rapid analysis and preparation of colostrum sialyloligosaccharides. J Chromatogr 212: 313–322, 1981

    Google Scholar 

  29. Martensson E, Raal A, Svennerholm L: Sialic acids in blood serum. Biochim Biophys Acta 30:124–129, 1958

    Google Scholar 

  30. Aminoff D: Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J 81: 384–392, 1961

    Google Scholar 

  31. Warren L: The thiobarbituric acid assay of sialic acids. J Biol Chem 234: 1971–1975, 1959

    Google Scholar 

  32. Sloneker JH: Gas liquid chromatography of alditol acetates. In: R.L. Whistler, J.N. BeMiller (eds). Methods of Carbohydrate Chemistry, vol. 6. Academic Press, New York and London, 1972, pp 20–24

    Google Scholar 

  33. Charnock-Jones DS, Sharkey AM, Fenwick P, Smith SK: Leukaemia inhibitory factor mRNA concentration peaks in human endometrium at the time of implantation and the blastocyst contains mRNA for the receptor at this time. J Reprod Fertil 101: 421–426, 1994

    Google Scholar 

  34. Watnick AS, Russo RA: Survival of skin homografts in uteri of pregnant and progesterone-estrogen treated rats. Proc Soc Exp Biol Med 128: 1–4, 1968

    Google Scholar 

  35. Roberts GP: Inhibition of lymphocyte stimulation by bovine uterine proteins. J Reprod Fertil 50: 337–339, 1977

    Google Scholar 

  36. Sherblom AP, Smagula RM, Moody CE, Anderson GW: Immunosuppresion, sialic acid, and sialyl transferase of bovine serum as a function of progesterone concentration. J Reprod Fertil 74: 509–517, 1985

    Google Scholar 

  37. Shaw L, Schauer R: The biosynthesis of N-glycoloylneuraminic acid occurs by hydroxylation of the CMP-glycoside of N-acetylneuraminic acid. Biol Chem Hoppe-Seyler 369: 477–486, 1988

    Google Scholar 

  38. Schauer R, Stoll S, Reuter G: Differences in the amount of N-acetyland N-glycoloylneuraminic acid, as well as O-acylated sialic acids, of fetal and adult bovine tissues. Carb Res 213: 353–359, 1991

    Google Scholar 

  39. Traving C, Schauer R: Structure, function and metabolism of sialic acids. Cell Mol Life Sci 54: 1330–1349, 1998

    Google Scholar 

  40. Schauer R, Malykh YN, Krisch B, Gollub M, Shaw L: Biosynthesis and biology of N-glycolylneuraminic acid. In: Y. Inoue, Y.C. Lee, F.A. Troy II (eds). Sialobiology and Other Novel Forms of Glycosylation. Gakushin Publishing Co., Osaka, 1999, pp 17–27

    Google Scholar 

  41. Muchmore EA: Developmental sialic acid modifications in rat organs. Glycobiol 2: 337–343, 1992

    Google Scholar 

  42. Bouhours J-F, Bouhours D: Hydroxylation of CMP-Neu5Ac controls the expression of N-glycolylneuraminic acid in GM3 ganglioside of the small intenstine of inbred rats. J Biol Chem 264: 16992–16999, 1989

    Google Scholar 

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Authors and Affiliations

  1. Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta, 700 032, India

    U. Chatterji

  2. Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta, 700 032, India

    A.K. Sen & M. Chowdhury

  3. Biochemisches Institut, Christian-Albrechts-Universität, Olshausenstrasse 40, D-24098, Kiel, Germany

    R. Schauer

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  1. U. Chatterji
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  3. R. Schauer
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  4. M. Chowdhury
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Chatterji, U., Sen, A., Schauer, R. et al. Paracrine effects of a uterine agglutinin are mediated via the sialic acids present in the rat uterine endometrium. Mol Cell Biochem 215, 47–55 (2000). https://doi.org/10.1023/A:1026582715752

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