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Radioimmunochemical studies for development of a solid-phase radioimmunoassay system using coated beads for the assessment of human chorionic gonadotropin

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Abstract

A method was developed for immobilization of prepared human chorionic gonadotropin (β-hCG) antisera on fresh plastic beads through a covalent immune bridge. Such covalent immune-coated antisera on plastic beads were used in the development of a noncentrifugation radioimmunoassay. 125I-β-hCG was prepared using different oxidizing agents. The solid-phase RIA system developed was found to well compete with the conventional method.

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Authors and Affiliations

  1. Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, P.O. Box 13959, Cairo, Egypt

    Kh. M. Sallam, A. S. A. El-Bayoumy & N. L. Mehany

Authors
  1. Kh. M. Sallam
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  2. A. S. A. El-Bayoumy
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  3. N. L. Mehany
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Corresponding author

Correspondence to A. S. A. El-Bayoumy.

Additional information

Published in Russian in Radiokhimiya, 2017, Vol. 59, No. 2, pp. 159–164.

The text was submitted by the authors in English.

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Sallam, K.M., El-Bayoumy, A.S.A. & Mehany, N.L. Radioimmunochemical studies for development of a solid-phase radioimmunoassay system using coated beads for the assessment of human chorionic gonadotropin. Radiochemistry 59, 181–187 (2017). https://doi.org/10.1134/S1066362217020126

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  • DOI: https://doi.org/10.1134/S1066362217020126

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