Callus production from protoplasts of Cyclamen persicum

Abstract

Cyclamen persicum (Mill.) cv. Sierra Rose protoplasts were isolated from adventitious shoots regenerated in vitro from petiole and leaf explants with yields of 1.3 × 106 protoplasts/g f.wt. of tissue. Protoplasts were embedded within agarose lenses bathed in modified KM8p medium supplemented with 1.0 mg l-1 NAA, 0.1 mg l-1 2,4-D and 0.5 mg l-1 BA. Cell division was observed after 4–5 days. After 6 weeks calli had grown out of the lenses which were transferred to modified MS medium for further callus growth. The most rapid callus growth was on medium containing 0.1 mg l-1 NAA and 10 mg l-1 TDZ.