Abstract
An efficient and reproducible plant regeneration system has been developed from seed-derived callus cultures of finger millet. Seeds of finger millet were cultured on Murashige and Skoog (MS) media supplemented with different concentrations of 2, 4-Dichlorophenoxyacetic acid (0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg l−1) and Benzyl amino purine (0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg l−1), to obtain organogenic calli. Highest callus induction frequency was obtained on MS media supplemented with 1.5 mg l−1 2, 4-Dichlorophenoxyacetic acid, and 1.5 mg l−1 of Benzyl amino purine. Varying degree of shoot regeneration, ranging from 0 to 64.2 %, was recorded at different concentrations of 2, 4-Dichlorophenoxyacetic acid (0.0 and 0.5 mg l−1) and Benzyl amino purine (0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg l−1) tested. Optimal multiple shoot induction response was recorded on MS media supplemented with 3.0 mg l−1 of Benzyl amino purine. Different concentrations of Naphthalene acetic acid (0.0, 1.0, 2.0 and 3.0 mg l−1) and Benzyl amino purine (0 and 1.0 mg l−1) were tested to induce root initiation response. Interestingly, optimum root induction was observed on MS basal media devoid of phyto-hormones, after 10 days of sub-culturing. Regenerated plants were transferred to pots containing coco-peat and maintained under controlled conditions for 3 weeks. Young plantlets were then transferred to green house and grown to maturity. Regenerated plants flowered after 65–70 days of transfer and seeds from the regenerated plants were collected at maturity.
Similar content being viewed by others
References
Bhatt D, Negi M, Sharma P, Saxena SC, Dobriyal AK, Arora S (2011) Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution. Physiol Mol Bio Plants 17:347–353
Borlaug NE (2002) Feeding a world of 10 billion people: The miracle ahead. In Vitro Cell Dev Biol Plant 38:221–228
Ceasar SA, Ignacimuthu S (2008) Efficient somatic embryogenesis and plant regeneration from shoot apex explants of different Indian genotypes of finger millet (Eleusine coracana Gaertn.). In Vitro Cell Dev Biol Plant 44:427–435
Kothari SL, Chandra N (1995) Advances in tissue culture and genetic transformation of cereals. J Indian Bot Soc 74:323–342
Kothari SL, Agarwal K, Kumar S (2004) Inorganic nutrient manipulation for highly improved in vitro plant Regeneration in finger millet–Eleusine coracana (l.) Gaertn. In vitro Cell Dev Biol Plant 40:515–519
Kothari SL, Kumar S, Vishnoi RK, Kothari A, Watanabe KN (2005) Applications of biotechnology for improvement of millet crops: review of progress and future prospects. Plant Biotechnol 22:81–88
Kumar S, Agarwal K, Kothari SL (2001) In vitro induction and enlargement of apical domes and formation of multiple shoots in finger millet, Eleusine coracana (L.) Gaertn and crowfoot grass, Eleusine indica (L.). Gaertn Curr Sci 81:1482–1485
Latha MA, Dasvantha RV, Venkateswara RK (2005) Production of transgenic plants resistant to leaf blast disease in finger millet (Eleusine coracana (L.) Gaertn.). Plant Sci 169:657–667
Malleshi NG, Desikachar HSR (1986) Studies on comparative malting characteristics of some tropical cereals and millets. J Inst Brew 92:174–176
Morrish F, Vasil V, Vasil IK (1987) Developmental morphogenesis and genetic manipulation in tissue and cell cultures of the Gramineae. Adv Genet 24:431–499
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioaasays with tobacco tissue cultures. Physiol Plant 15:473–497
Rangan TS (1976) Growth and plantlet regeneration in tissue culture of some Indian millets: Papsalum scorbiculatum (L.); Eleusine coracina (L.) Gaertn. and Pennisetum typhoideum Pers. Pflawzenphysiol 78:208–216
Sivadas P, Kothari SL, Chandra N (1990) High frequency embryoid and plantlet formation from tissue culture of the finger millet- Eleusine coracana (L). Gaertn Plant Cell Rep 9:93–96
Thiru AN, Mohan Ram HY (1990) Tissue culture studies in Ragi Eleusine coracana (L.) Gaertn. Plant Cell Rep 9:93–96
Wakhlu AK, Bhau B (2000) Callus formation and plant regeneration from tubercules of Coryphanta elephantidens (Lem.) Lem. In Vitro Cell. Dev Biol Plant 36:211–214
Acknowledgments
We are thankful to the Department of Biotechnology, Government of India, for funding this work under the Program Mode Support in Agricultural Biotechnology initiative.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pande, A., Dosad, S., Chawla, H.S. et al. In-vitro organogenesis and plant regeneration from seed-derived callus cultures of finger millet (Eleusine coracana). Braz. J. Bot 38, 19–23 (2015). https://doi.org/10.1007/s40415-014-0102-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40415-014-0102-1