Abstract
A simple and efficient method for high frequency somatic embryogenesis and plant regeneration from hypocotyl-derived cultures and suspension cultures of Gossypium klotzschianum Anderss, a wild, diploid species of cotton is described here. Embryogenic cultures were induced from hypocotyl sections on MSB medium with 0.9 μM 2,4-D and 2.32 μM kinetin. MSB medium containing 0.045 μM 2,4-D, 0.93 μM kinetin, 2.46 μM IBA promoted embryogenic culture proliferation and embryo development. Suspension cultures with 0.23 μM 2,4-D and 0.93 μM kinetin also produced many embryos. Somatic embryos cultured on MSB medium with PGRs produced secondary embryos, and embryos developed into normal plantlets on PGR-free MSB medium. Regenerated plantlets were transferred onto the quarter-strength MSB medium with 0.5% active charcoal to avoid recallusing. Hypocotyls were better than cotyledons for culture induction and plant regeneration. 2,4-D and kinetin were essential for culture induction and maintenance.
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References
Davidonis GH & Hamilton RH (1983) Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Sci. Lett. 32: 89–93
Finer JJ & Smith RH (1984) Initiation of callus and somatic embryos from explants of mature cotton (Gossypium klotzschianum Anderss.). Plant Cell Rep. 3: 41–43
Firoozabady E & Deboer DL (1993) Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsunum L.). In Vitro Cell. Dev. Biol. 29: 166–173
He DY & Li YZ (1998) Somatic embryogenesis and plant regeneration in Gossypium hirsutum L. cv. ZhongMian 12. Acta Agriculturae Nucleatae Sinica 12: 332–336
Price HJ & Smith RH (1979) Somatic embryogenesis in suspension cultures of Gossypium klotzschianum Anderss. Planta 145: 305–307
Shoemaker RC, Couche LJ & Galbraith DW (1986) Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep. 3: 178–181
Trolinder NL & Goodin JR (1987) Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep. 6: 231–234
Trolinder NL & Xhixian C (1989) Genotype specificity of the somatic embryogenesis response in cotton. Plant Cell Rep. 8: 133–136
Zhang BH & Li XL (1995) High frequency somatic embryogenesis and plant regeneration of elite cotton variety. Acta Agriculturae Boreall-occidentalis Sinica 4: 11–16
Zhang XL, Sun JZ & Liu JL (1991) Somatic embryogenesis and plant regeneration in upland cotton. Acta Genetic Sinica 18: 461–467
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Sun, Y., Zhang, X., Jin, S. et al. Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum). Plant Cell, Tissue and Organ Culture 75, 247–253 (2003). https://doi.org/10.1023/A:1025895631247
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DOI: https://doi.org/10.1023/A:1025895631247