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Chromosome Organization and Differential Banding in Endomitotic Nuclei of Nurse Cells of Calliphora erythrocephala (Diptera: Calliphoridae)

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Abstract

The structure of primary polytene chromosomes and general architecture of nurse cell nuclei was studied in Calliphora erythrocephala using various methods of differential chromosome banding(G-, R-, C-banding; Ag-, and DAPI staining), chromospecific DNA probes and fluorescence in situ hybridization. This analysis revealed differential compaction of particular chromosome regions. The localization of material of polytene chromosome 6 is retained after its rearrangement and the formation of the internal reticular structure of the nucleus. Polytene chromosomes of ovarian nurse cells were shown to have blocks of dense compact material; some of them were more intensely stained by AgNO3. The dynamics of the nucleolus formation was traces at all stages of chromosome polytenization in the C. erythrocephala nurse cells.

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Authors and Affiliations

  1. Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, 634050, Russia

    I. A. Wasserlauf, T. V. Ananina, M. F. Unger, N. N. Melnikova & V. N. Stegnii

  2. Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    T. V. Karamysheva & N. B. Rubtsov

Authors
  1. I. A. Wasserlauf
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  2. T. V. Ananina
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  3. M. F. Unger
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  4. T. V. Karamysheva
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  5. N. N. Melnikova
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  6. N. B. Rubtsov
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  7. V. N. Stegnii
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Wasserlauf, I.A., Ananina, T.V., Unger, M.F. et al. Chromosome Organization and Differential Banding in Endomitotic Nuclei of Nurse Cells of Calliphora erythrocephala (Diptera: Calliphoridae). Russian Journal of Genetics 39, 1004–1012 (2003). https://doi.org/10.1023/A:1025718916613

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