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Genetic mapping of sequence-specific PCR-based markers on the short arm of the 1BL.1RS wheat-rye translocation

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Abstract

Wheat cultivars carrying the 1BL.1RStranslocation were crossed with newly synthesised octoploid triticale lines involving four rye genotypes having ο-secalin banding patterns different from each other and from that of the 1BL.1RS translocation. Homologous recombination was expected between the short arm of the 1R chromosomes of the rye genotypes and the 1RS arm of the 1BL.1RSwheat/rye translocation. Seven sequence-specific PCR-based markers:Xiag95, RMS13, Bmac0213, GPI, Xpsr960, 5Sand SCM9, and ο-secalinproteins were used to detect recombination events in the BC1F2 generation. Segregation analysis demonstrated that a barley SSR marker (Bmac0213) locus was present on the 1RS chromosome arm. Of 834plants tested in four different BC1F2 populations, 246individuals were found to carry recombined1BL.1RS translocation chromosomes. Genetic linkage analysis was performed on the eight markers in the four different mapping populations. The physical positions of the markers are discussed.

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Authors and Affiliations

  1. Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Brunszvik Str. 2, H-2462, Hungary

    E.D. Nagy & M. Molnár-Láng

  2. Institute for Agrobiotechnology, Tulln, Konrad Lorenz Str. 20, A-3430, Austria

    C. Eder & T. Lelley

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  1. E.D. Nagy
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  2. C. Eder
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  3. M. Molnár-Láng
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  4. T. Lelley
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Nagy, E., Eder, C., Molnár-Láng, M. et al. Genetic mapping of sequence-specific PCR-based markers on the short arm of the 1BL.1RS wheat-rye translocation. Euphytica 132, 243–249 (2003). https://doi.org/10.1023/A:1025002919746

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