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Assembly of the photosynthetic apparatus in embryos from Fucus serratus L

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Abstract

The assembly of the photosynthetic apparatus was studied during the first six days of development of Fucus serratus L. embryos. HPLC analysis revealed that oospheres and zygotes contain the same photosynthetic pigments (i.e., chlorophyll a, chlorophyll c, fucoxanthin, violaxanthin, and β-carotene) as fully developed thalli. Total pigment amount increased after fertilization, mainly due to an active synthesis of Chl a and fucoxanthin. Spectral modifications revealing the progressive integration of Chl a and Chl c in the photosynthetic units are described. In particular, a distinct emission at 705 nm, reflecting the accumulation of LHC I, was clearly detected. The emission bands at 705 nm and 725 nm were characterized by 77 K excitation fluorescence measurements. Their spectra differed by the presence of a large band at approximately 550 nm due to fucoxanthin in the excitation spectrum of F705 nm. Room temperature variable fluorescence was first observed 30 h after fertilization indicating a functional Photosystem II electron transfer at this developmental stage.

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Author notes

  1. Benoît Schoefs

    Present address: Phytobiologie Cellulaire, UMR-INRA/UB1088 – FRE-CNRS 2625 – Plante-Microbe-Environnement, Université de Bourgogne à Dijon, BP 47870, 21078, Dijon Cedex, France

Authors and Affiliations

  1. Cytophysiologie Végétale et Phycologie, UPRESA-CNRS 8013, Université de Lille 1, 659655, Villeneuve d'Ascq, France

    Morgane Lamote, Eva Darko & Yves Lemoine

  2. Laboratoire d'Ecologie et Phycologie, Université, Laval, Québec, G1K 7P4, Canada

    Morgane Lamote

  3. Ecole Normale Supérieure, CNRS – UMR 8543, Dynamique des Membranes Végétales, Complexes Protéines-Pigments, 46 rue d'Ulm, 75230, Paris Cedex 5, France

    Benoît Schoefs

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  1. Morgane Lamote
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  4. Yves Lemoine
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Correspondence to Benoît Schoefs.

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Lamote, M., Darko, E., Schoefs, B. et al. Assembly of the photosynthetic apparatus in embryos from Fucus serratus L. Photosynthesis Research 77, 45–52 (2003). https://doi.org/10.1023/A:1024999024157

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