Abstract
The nitric oxide precursor L-arginine (ARG) has been shown to influence intestinal morphology and intestinal absorptive function. The purpose of the present study was to determine the effect of enteral ARG supplementation on structural intestinal adaptation, cell proliferation, and apoptosis in a rat model of short bowel syndrome (SBS). Thirty male Sprague-Dawley rats were divided into three experimental groups: Sham rats underwent bowel transection, SBS rats underwent 75% small bowel resection, and SBS-ARG rats underwent bowel resection and were treated with ARG given in the drinking water (2%). Parameters of intestinal adaptation, enterocyte proliferation and enterocyte apoptosis were determined on day 14 following operation. We have demonstrated that SBS-ARG animals had a lower jejunal and ileal mucosal weight, jejunal mucosal DNA and protein, ileal mucosal protein, jejunal villus height, jejunal and ileal crypt depth, and enterocyte proliferation index and a greater enterocyte apoptosis compared to SBS untreated animals. We conclude that in a rat model of SBS enteral L-arginine inhibits structural intestinal adaptation. Possible mechanism for this effect may be decreased cell proliferation and increased cell apoptosis.
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Beale RJ, Bryg DJ, Bihari DJ: Immunonutrition in the critically ill: a systematic review of clinical outcome. Crit Care Med 27:2799-2805, 1999
O'Flaherty L, Bouchier-Hayes DJ. Immunonurition and surgical practice: Proc Nutr Soc 58:831-837, 1999
Evoy D, Lieberman MD, Fahey TJ, Daly JM: Immunonutrition: the role of arginine. Nutrition 14:611-617, 1998
Chung HT, Pae HO, Choi BM, Billiar TR, Kim YM: Nitric oxide as a bioregulator of apoptosis. Biochem Biophys Res Commun 282:1075-1079, 2001
Welters CFM, Dejong CHC, Deutz NEP, Heinemang E: Effects of parenteral arginine supplementation on the intestinal adaptative response after massive small bowel resection in the rat. J Surg Res 85:259-266, 1999
Dowling RH, Booth CC: Structural and functional changes following small intestinal resection in the rats. Clin Sci 32:139-149, 1967
Chomszynski P: A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Bio Techniques 15:532-536, 1993
Kvietys PR: Intestinal physiology relevant to short-bowel syndrome. Eur J Pediatr Surg 9:196-199, 1999
Booth IW, Lander AD: Short bowel syndrome. Bailliere's Clin Gastroenterol 12:739-772, 1998
O'Brien DP, Nelson LA, Huang FS, Warner BW: Intestinal adaptation: structure, function, and regulation. Sem in Pediatr Surg 10:55-64, 2001
Vanderhoof JA, Young RJ: Enteral nutrition in short bowel syndrome. Sem in Pediatr Surg 10:65-71, 2001
Wu G, Morris SM: Arginine metabolism: nitric oxide and beyond. Biochem J 336:1-17, 1998
Cynober L, Le Boucher J, Vasson MP: Arginine metabolism in mammals. Nutr Biochem 6:402-413, 1995
Wapnir RA, Wingertzahn MA, Teichberg S: L-Arginine in low concentration improves rat intestinal water and sodium absorption from oral rehydration solutions. Gut 40:602-607, 1997
Thomas S, Anup R, Susama P, Balasubramanian KA: Nitric oxide prevents intestinal mitochondrial dysfunction induced by surgical stress. Br J Surg 88:393-399, 2001
Sukumar P, Loo A, Magur E, Nandi J, Oler A, Levine RA: Dietary supplementation of nucleotides and arginine promotes healing of small bowel ulcers in experimental ulcerative ileitis. Dig Dis Sci 42:1530-1536, 1997
Gurbuz AT, Kunzelman J, Ratzer EE: Supplemental dietary argininenaccelerates intestinal mucosal regeneration and enhances bacterial clearance following radiation enteritis in rats. J Surg. Res 74:149-154, 1998
Schleiffer R, Raul F: Prophylactic adminstration of L-arginine improves the intestinal barrier function after mesenteric ischemia. Gut 39:194-198, 1996
Hebiguchi T, Kato T, Yoshino H, Mizuno M, Koyama K: The effect of arginine supplementation on growth hormone release and intestinal mucosal growth after massive small bowel resection in growing rats. J Pediatr Surg 32:1149-1153, 1997
Czernicow B, Nsi-Emvo E, Galluser M, Gosse F, Raul F: Enteral supplementation with ornithine alpha ketaglutarate improves the early adaptive response to resection. Gut 40:67-72, 1997
Sukhotnik I, Krausz MM, Sabo E, Resnick M, Hirsh M, Mannheim D, Shiloni E: Endotoxemia inhibits intestinal adaptation in a rat model of short bowel syndrome. Shock (in press)
Sukhotnik I, Khateeb K, Krausz MM, Sabo E, Siplovich L, Coran AG, Shiloni E: Sandostatin impairs post-resection intestinal adaptation in a rat model of short bowel syndrome. Dig Dis Sci 47:2095-2102, 2002
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Sukhotnik, I., Lerner, A., Sabo, E. et al. Effects of Enteral Arginine Supplementation on the Structural Intestinal Adaptation in a Rat Model of Short Bowel Syndrome. Dig Dis Sci 48, 1346–1351 (2003). https://doi.org/10.1023/A:1024167428092
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DOI: https://doi.org/10.1023/A:1024167428092