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Effect of Smoke, Charred Wood, and Nitrogenous Compounds on Seed Germination of Ten Species from Woodland in Central-Western Spain

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Abstract

The effect of smoke, charred wood, and nitrogenous compounds on germination was tested on 10 species of the Cistaceae, Poaceae, Fabaceae, and Asteraceae, from fire-prone, shrubby woodlands in central-western Spain. Dry seeds were exposed to smoke, by watering with distilled water–charred wood suspensions, or NaNO2, KNO3, NH4Cl, and NH4NO3. Smoke enhanced germination in 9 of 10 of the species. In species of Poaceae, germination was stimulated by 20 min of smoke exposure. In Asteraceae and Fabaceae species, 10 min of smoke exposure was the most effective treatment for enhancing germination. Three species—Cistus ladanifer, Cistus crispus, and Cistus monspeliensis—had a positive response to 20 min of smoke exposure; germination of Cistus salviifolius L. was also enhanced after 10 min. The effect of charred wood was variable, with no consistent germination pattern within the families. Trifolium angustifolium and Retama sphaerocarpa showed no stimulation of germination under most of the charred wood concentrations. Similarly, germination of Senecio jacobea under the charred wood treatment did not surpass that of the control. NaNO2 promoted seed germination in Dactylis glomerata (10 mM), Cistus ladanifer (1, 10, and 25 mM), and Cistus crispus (1 and 10 mM). KNO3 enhanced germination in Dactylis glomerata (1 and 25 mM), Dittrichia viscosa (10 and 25 mM), C. ladanifer (1, 10, and 25 mM), Cistus crispus (1 and 25 mM), and C. salviifolius aud C. monspeliensis (25 mM). NH4Cl induced germination of Dactylis glomerata and Dittrichia viscosa (1 mM), and Cistus species germinated best in 25 mM of this salt. NH4NO3 induced germination only in Cistus species. Holcus lanatus had the highest level of germination regardless of treatment.

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References

  • Añorbe-Urmeneta, M., Gómez-Gutiérrez, J. M., Pérez-Fernández, M. A., and Fernández-Santos, B. 1990. Influencia de la temperatura sobre la germinación de semillas de Cytisus multiflorus (L'Hér) Sweet y Cytisus oromediterraneus Riv. Mar. Stud. Oecol. 7:85-100.

    Google Scholar 

  • Aronne, G. and Mazzoleni, S. 1989. The effects of heat exposure on seeds of Cistus incanus L. and Cistus monspeliensis. L. G. Bot. Ital. 123:282-289.

    Google Scholar 

  • Auld, T. D. 1986. Population dynamics of the Acacia suaveolens (Sm.) Willd: fire and the transition to seedlings. Aust. J. Ecol. 11:373-385.

    Google Scholar 

  • Baldwin, I. T., Staszak-Kozinski, L., and Davidson, R. 1994. Up in smoke: I. Smoke-derived germination cues for the postfire annual Nicotiana attenuata Torr. ex. Watson. J. Chem. Ecol. 20:2345-2371.

    Google Scholar 

  • Baskin, J. M. and Baskin, C. C. 1989. Physiology of dormancy and germination in relation to seed bank ecology, pp. 53-66, in M. A. Leck, V. T. Thomas, and R. L. Simpson (Eds.). Ecology of Soil Seed Banks. Academic Press, San Diego, California.

    Google Scholar 

  • Baxter, B. J. M., Van Staden, J., Ganger, J. E., and Brown, N. A. C. 1994. Plant-derived smoke and smoke extracts stimulate seed germination of the fire climax grass, Themeda triandra. Environ. Exp. Bot. 34:217-223.

    Google Scholar 

  • Bell, D. T., Plummer, J. A., and Taylor, S. K. 1993. Seed germination ecology in southwestern Western Australia. Bot. Rev. 76:24-73.

    Google Scholar 

  • Bell, D. T., King, L. A., and Plummer, J. 1999. Ecophysiological effects of light quality and nitrate on seed germination in species from Western Australia. Aust. J. Ecol. 24:2-10.

    Google Scholar 

  • Bradstock, R. A. and Auld, T. D. 1995. Soil temperatures during experimental bushfires in relation to fire intensity: consequences for legume germination and fire management in south-eastern Australia. J. Appl. Ecol. 32:76-84.

    Google Scholar 

  • Christensen, N. L. and Muller, C. H. 1975. Effects of fire on factors controlling plant growth in Adenostoma chaparral. Ecol. Monogr. 45:29-55.

    Google Scholar 

  • Craston, H. J., Kern, A. J., Gerhardt, S. A., and Dyer, W. E. 1996. Wound-induced ethylene and germination of embryos excised from dormant Avena fatua L. caryopses. Int. J. Plant Sci. 157:153-158.

    Google Scholar 

  • Cochrane, A., Brown, K., Meeson, N., and Harding, C. 1999. The germination requirements of Hemigenia exilis (Lamiaceae)—seed plug removal and gibberellic acid as a successful technique to break dormancy in an arid zone shrub from Western Australia. CALM Sci. 3:21-30.

    Google Scholar 

  • De Lange, J. H., and Boucher, C. 1990. Autoecological studies on Audouinia capitata (Bruniaceae). I. Plant-derived smoke as a seed germination cue. S. Afr. J. Bot. 56:700-703.

    Google Scholar 

  • Devesa, J. A. 1995. Vegetación y flora de extremadura. Universitas Editorial. Badajoz, Spain.

    Google Scholar 

  • Dixon, K. W., Roche, S., and Pate, J. S. 1995. The promotive effect of smoke derived from burnt native vegetation on seed germination of Western Australian plants. Oecologia 101:185-192.

    Google Scholar 

  • Egerton-Warburton, L. M. 1998. A smoke-induced alteration of the sub-testa cuticule in seeds of the post-fire recruiter Emmenanthe penduliflora Benth. (Hydrophyllaceae). J. Exp. Bot. 49:1317-1327.

    Google Scholar 

  • Enright, N. J., Goldblum, D., Ata, P., and Ashton, D. H. 1997. The independent effects of heat, smoke and ash on emergence of seedlings from the soil seed bank of a heathy Eucalyptus woodland in Grampians (Gariwerd) National Park, Western Victoria. Aust. J. Ecol. 2:81-88.

    Google Scholar 

  • Fernández-Santos, B. and Gómez-Gutiérrez, J. M. 1994. Changes in Cytisus balansae populations after fire. J. Veg. Sci. 5:463-472.

    Google Scholar 

  • Ferrandis, P., Herranz, J. M., and Martínez-Sánchez, J. J. 1999. Effect of fire on hard-coated Cistaceae seed banks and its influence on techniques for quantifying seed banks. Plant Ecol. 144:103-114.

    Google Scholar 

  • Franco-Vizcaíno, E. and Sosa-Ramírez, J. 1997. Soil properties and nutrient relations in burned and unburned Mediterranean-climate shrublands of Baja California, Mexico. Acta Oecol. 18:503-517.

    Google Scholar 

  • Gómez-Gutiérrez, J. M. and Pérez-Fernández, M. A. 1996. The “dehesas,” silvopastoral systems in semiarid Mediterranean regions with poor soils, seasonal climate and extensive utilization, pp. 55-71, in M. Etienne (ed.). Western European Silvopastoral Systems. INRA, Paris.

    Google Scholar 

  • Gónzález-Rabanal, F. and Casal, M. 1995. Effects of high temperatures and ash on germination of ten species from gorse shrubland. Vegetatio 116:123-131.

    Google Scholar 

  • Hsiao, A. I., Mcintyre, G. I., and Hanes, J. A. 1983. Seed dormancy in Avena fatua. I. Induction of germination by mechanical injury. Bot. Gaz. 144:217-222.

    Google Scholar 

  • Humphreys, F. R. and Craig, F. G. 1981. Effects of fire on soil chemical, structural and hydrological properties, pp. 177-200, in A. M. Gill, R. H. Groves, and I. R. Noble (Eds.). Fire and the Australian Biota. Australian Academy of Science, Canberra.

    Google Scholar 

  • ISTA (International Seed Testing Association). 1993. International Rules for Seed Testing. Proc. Int. Seed Test. Assoc. 21(Suppl.):160-186.

    Google Scholar 

  • Keeley, J. E. 1987. Role of fire in seed germination of woody taxa in California chaparral. Ecology 68:434-443.

    Google Scholar 

  • Keeley, J. E. 1991. Seed germination and life history syndromes in California chaparral. Bot. Rev. 57:81-116.

    Google Scholar 

  • Keeley, J. E. and Fotheringham, C. J. 1997. Trace gas emissions and smoke-induced seed germination. Science 276:1248-1250.

    Google Scholar 

  • Keeley, J. E. and Fotheringham, C. J. 1998. Mechanisms of smoke-induced seed germination in a post-fire chaparral annual. J. Ecol. 86:27-36.

    Google Scholar 

  • Kruger, F. J. and Bigalke, R. C. 1984. Fire in fynbos, pp. ? in P. de V. Booysen and N. M. Tainton (Eds.). Ecological Effects of Fire in South African Ecosystems. Springer-Verlag, Berlin pp. 12-29.

    Google Scholar 

  • Linington, S., Mkhonta, D., Pritchard, H. W., and Terry, J. 1994. A provisional germination testing scheme for seed of the compositae, pp. 223-235, in P. D. S. Caligari and D. J. N. Hind (Eds.). Compositae: Biology and Utilization. Proceedings of the International Compositae Conference, Kew. Royal Botanic Gardens, Kew, United Kingdom.

    Google Scholar 

  • López González, G. 1995. La guía INCAFO de los árboles y arbustos de la Península Ibérica. Guías Verdes Incafo, 5 ed. Jardín Botánico, CSIC, Madrid.

    Google Scholar 

  • LymanO. R. 1998. An Introduction to Statistical Methods and Data Analysis, 4th ed. Duxbury Press, United State, pp. 12-19.

    Google Scholar 

  • Malcolm, A. and Williams, J. E. 1996. Fire regimes and biodiversity: the effects of fragmentation of southeastern Australian eucalypt forests by urbanisation, agriculture and pine plantations. For. Ecol. Manage. 85:261-278.

    Google Scholar 

  • Matlack, G. R., Gibson, D. J., and Good, R. E. 1993. Regeneration of the shrub Gaylussacia baccata and associated species after long intensity-fire in an Atlantic coastal plain forest. Am. J. Bot. 80:119-126.

    Google Scholar 

  • Mayer, A. M. and Poljakoff-Mayber, A. 1989. The Germination of Seeds. Pergamon Press, Oxford.

    Google Scholar 

  • Pérez-Fernández, M. A., Gómez Gutiérrez, J. M., and Martín Berrocoso, A. M. 1996. Comportamiento germinativo de especies de matorral autóctono, sembrados en suelos de diferentes características. ITEA 7:386-395.

    Google Scholar 

  • Pérez-Fernández, M. A., Lamont, B. B., Marwick, A. L., and Lamont, W. G. 2000. Germination of seven exotic weeds and seven native species in south-western Australia under steady and fluctuating water supply. Acta Oecol. 21:323-336.

    Google Scholar 

  • Pierce, S. M., Esner, K., and Cowling, R. 1995. Smoke-induced germination of succulents (Mesembryanthemaceae) from fire-prone and fire-free habitats in South Africa. Oecologia 102:520-522.

    Google Scholar 

  • Plummer, J. A., Mcchesney, C. J., and Bell, D. T. 1997. Germination in photosensitive seeds: phytochrome stimulates metabolism of gibberellins A19 and A20 to GA1. Aust. J. Plant Physiol. 24:389-394.

    Google Scholar 

  • Pons, T. L. 1989. Breaking of seed dormancy by nitrate as a gap detection mechanism. Ann. Bot. 63:139-143.

    Google Scholar 

  • Portlock, C. C., Shea, S. R., Majer, J. D., and Bell, D. T. 1990. Stimulation of germination of Acacia pulchella: laboratory basis for forest management options. J. Appl. Ecol. 27:319-324.

    Google Scholar 

  • Pugnaire, F. I. and Lozano, J. 1997. Effects of soil disturbance, fire and litter accumulation on the establishment of Cistus clusii seedlings. Plant Ecol. 131:207-213.

    Google Scholar 

  • Read, T. R., Bellairs, S. M., Mulligan, D. R., and Lamb, D. 2000. Smoke and heat effects on soil seed bank germination for the reestablishment of a native forest community in New South Wales. Aust. Ecol. 25:48-57.

    Google Scholar 

  • Roche, S., Dixon, K. W., and Pate, J. 1998. For everything a season: smoke-induced seed germination and seedling recruitment in a Western Australian Banksia woodland. Aust. J. Ecol. 23:111-120.

    Google Scholar 

  • Sanders, D., Hansen, U. P., and Slayman, C. L. 1981. Role of the plasma membrane proton pump in pH regulation in non-animal cells. Proc. Natl. Acad. Sci. USA 78:5903-5907.

    Google Scholar 

  • Schatral, A. 1996. Dormancy in seeds of Hibbertia hypericoides (Dilleniaceae). Aust. J. Bot. 44:213-222.

    Google Scholar 

  • Taylor, J. L. S. and Van Staden, J. 1996. Root initiation in Vigna radiata (L.) Wilczek hypocotyl cuttings is stimulated by smoke-derived extractas. Plant Growth Regul. 18:165-168.

    Google Scholar 

  • Thanos, C. A. and Rundel, P. W. 1995. Fire-followers in chaparral: nitrogenous compounds trigger seed germination. J. Ecol. 83:207-216.

    Google Scholar 

  • Thanos, C. A. and Georghiou, K., Kadis, C., and Pantazi, C. 1992. Cistaceae: a family with hard seeds. Isr. J. Bot. 41:251-263.

    Google Scholar 

  • Trabaud, L. 1984. Postfire plant community dynamics in Mediterranean basin pp. in J. M. Moreno and W. C. Oechel, (Eds.). The Role of Fire in Mediterranean-Type Ecosystems. Springer-Verlag, New York. pp. 101-116.

    Google Scholar 

  • Tutin, T. G., Heywood, V. H. Burges, N. A., Moore, D. M., Valentine, D. H., Walters, S. M., and Wedd, D. A. pp. 1964-1980 in Flora Europea. Cambridge University Press, Cambridge.

  • Valbuena, L., Tárrega, R., and Luis, E. 1992. Influence of heat on seed germination of Cistus laurifolius and Cistus ladanifer. Int. J. Wildl. Fire 2:15-20.

    Google Scholar 

  • Voesenek, L. A. C. J. and Blom, C. W. P. M. 1996. Plants and hormones: An ecophysiological view on timing and plasticity. J. Ecol. 84:111-119.

    Google Scholar 

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  1. Ecology Department, University of Extremadura, Avenida de Elvas s/n, 06071, Badajoz, Spain

    M. A. Pérez-Fernández & S. Rodríguez-Echeverría

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  1. M. A. Pérez-Fernández
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Correspondence to M. A. Pérez-Fernández.

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Pérez-Fernández, M.A., Rodríguez-Echeverría, S. Effect of Smoke, Charred Wood, and Nitrogenous Compounds on Seed Germination of Ten Species from Woodland in Central-Western Spain. J Chem Ecol 29, 237–251 (2003). https://doi.org/10.1023/A:1021997118146

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