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Selection of a Potent Bacillus licheniformis Strain Producing Thermostable Amylase

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Abstract

A highly potent strain of Bacillus licheniformis 103 that synthesized thermostable α-amylase with temperature and pH optima of 90–95°C and 6.0–8.5, respectively, was obtained by mutagenesis and selection. The composition of fermentation media and conditions for submerged cultivation of the producer were optimized. α-Amylase whose activity reached 260 U/ml was obtained in laboratory fermenters.

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Authors and Affiliations

  1. All-Russia Institute of Food Biotechnology, Russian Academy of Agricultural Sciences, Moscow, 109033, Russia

    N. V. Tsurikova, L. I. Nefedova & E. V. Kostyleva

  2. State Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545, Russia

    V. I. Zvenigorodskii, V. G. Yasinovskii & T. A. Voeikova

  3. Department of Chemistry, Moscow State University, Moscow, 119899, Russia

    A. P. Sinitsyn

Authors
  1. N. V. Tsurikova
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  2. L. I. Nefedova
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  3. E. V. Kostyleva
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  4. V. I. Zvenigorodskii
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  5. V. G. Yasinovskii
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  6. T. A. Voeikova
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  7. A. P. Sinitsyn
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Tsurikova, N.V., Nefedova, L.I., Kostyleva, E.V. et al. Selection of a Potent Bacillus licheniformis Strain Producing Thermostable Amylase. Applied Biochemistry and Microbiology 38, 427–432 (2002). https://doi.org/10.1023/A:1019960216770

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  • DOI: https://doi.org/10.1023/A:1019960216770

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