Abstract
Rutacridone, alkaloid from plant Ruta graveolens was used for the histochemical staining of Hippeastrum hybridum pollen. The rutacridone fluorescence (excited by UV light at 360–380 nm) has been studied with Karnaukhov-constructed microspectrofluorimeters. Nucleus and DNA were stained by this dye in cells and fluoresced by green light with maxima of 530 and 555 nm, respectively. Development of the pollen was analyzed with this dye.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
E. Kranz and T. Dresselhaus (1996) Trends in Plant Sci. 1, 82–89.
E. Kranz (2001) in S. S. Bhojwani and W. Y. Soh (Eds.) Current Trends in the Embryology of Angiosperms, Kluwer, Dordrecht, pp 143–166.
A. Baumert, I. N. Kuzovkina, G. Krauss, M. Hieke, and D. Grogewer (1982) Plant Cell Rep. 1, 168–171.
V. N. Karnaukhov (1978) Lumenescent Spectral Analysis of Cells, Nauka, Moskow.
V. N. Karnaukhov, V. A. Yashin, A. P Kazantsev, N. A Karnaukhova, and V. I. Kulakov (1987) Tsitologia (Cytology, Russia) 29, 113–116.
V.V. Roshchina, E.V. Melnikova, and L.V. Kovaleva (1997) Russian J. Plant Physiol. 44, 45–53
V. V. Roshchina and E. V. Melnikova (2001) Russian Plant Physiol. 48(1), 89–99.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roshchina, V.V. Rutacridone as a Fluorescent Dye for the Study of Pollen. Journal of Fluorescence 12, 241–243 (2002). https://doi.org/10.1023/A:1016821119573
Issue Date:
DOI: https://doi.org/10.1023/A:1016821119573