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Analysis of Heat Shock Proteins and Thermotolerance in a Thermoresistant Strain of Drosophila melanogaster

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Abstract

Here we studied the response to heat shock in a desert D. melanogasterstrain TT capable of living and propagating at 32°C and the standard Oregon R strain. The TT strain proved to be more resistant to extreme temperatures. On the other hand, the observed high thermotolerance of the strain was not accompanied by a higher level of HSP70 synthesis. Conversely, reliably smaller amounts of HSP70 were synthesized in the TT strain as compared to Oregon R under all shock temperatures except the critical one (39.5°C). Differences in both the structure of HSP70genes and the pattern of all heat shock proteins have been observed between the studied strains. The role of the heat shock system in the adaptation to hyperthermia is discussed.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 119991, Russia

    V. B. Molodtsov, D. G. Garbuz & O. G. Zatsepina

  2. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142292, Russia

    V. V. Velikodvorskaya & M. B. Evgen'ev

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  1. V. B. Molodtsov
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  2. V. V. Velikodvorskaya
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  3. D. G. Garbuz
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  4. O. G. Zatsepina
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  5. M. B. Evgen'ev
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Molodtsov, V.B., Velikodvorskaya, V.V., Garbuz, D.G. et al. Analysis of Heat Shock Proteins and Thermotolerance in a Thermoresistant Strain of Drosophila melanogaster. Biology Bulletin 28, 439–448 (2001). https://doi.org/10.1023/A:1016783908571

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