Abstract
Leptin, an adipose tissue-derived of gene product, is important in energy metabolism. However, the role of leptin in the metabolism of lipids is still not clear in humans. The purpose of this study was to evaluate the association of plasma leptin concentrations and lipid profiles among school children in Taiwan. After multistage sampling of 85 junior high schools in Taipei, we randomly selected 1264 children (617 boys and 647 girls) aged 12–16 years for this study. We measured the anthropometric variables, lifestyle factors and biochemical parameters among these children. Anthropometric measurements included body height (BH) and weight (BW) and we calculated body mass index (BMI) as the ratio of the BW to the square of the BH, expressed in kg/m2. Plasma leptin levels were measured by radioimmunoassay. We also measured lipid profiles including serum total cholesterol (CHOL), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), apolipoprotein-A1 (Apo-A1), apolipoprotein-B (Apo-B), and lipoprotein(a) (Lp(a)) levels, and calculated low density lipoprotein-cholesterol (LDL-C) levels and CHOL to HDL-C ratio (TCHR). Girls had higher leptin, CHOL, TG, HDL-C, (LDL-C), Apo-A1, Apo-B, and Lp(a) levels and lower BMI than boys did. Plasma leptin concentrations were significantly positively correlated with TG, LDL-C, and Apo-B, but negatively with HDL-C and Apo-A1 in both the genders. Children with higher plasma leptin levels (>75th percentiles) have significantly higher TG, HDL-C, LDL-C, TCHR, and Apo-B than those with relatively lower leptin levels. In multivariate regression analyses, the association between plasma leptin level and lipid profiles (such as CHOL, TG, and Apo-B) were still significant (p < 0.05) even after adjusting for BMI among boys. However, this association became attenuated and insignificant among girls. Finally, in the model that included the standard covariates, plasma leptin was the most predictive of CHOL, TG and Apo-B levels among those school children in Taiwan. Our results suggest that plasma leptin and BMI were independently associated with the lipids and lipoprotein profiles among Taiwanese Children. In both genders, children in the top 25% of the leptin distribution have more adverse lipid and lipoprotein profiles.
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Castelli WP, Garrison RJ, Wilson PW, et al. Incidence of coronary heart disease and lipoprotein cholesterol levels: The Framingham Study. JAMA 1986; 256: 2835–2838.
Manninen V, Tenkanen L, Koskinen P, et al. Joint e.ects of serum triglyceride and LDL cholesterol and HDL cholesterol concentrations on coronary heart disease risk in the Helsinki Heart Study. Implications for treatment. Circulation 1992; 85: 37–45.
Assmann G, Schulte H, Von Eckardstein A. Hypertriglyceridemia and elevated lipoprotein (a) are risk factors for major coronary events in middle-aged men. Am J Cardiol 1996; 7: 1179–1184.
Kikuchi DA, Srinivasan SR, Harsha DW, Webber LS, Sellers TA, Berenson GS. Relation of serum lipoprotein lipids and apolipoproteins to obesity in children: The Bogalusa Heart Study. Prev Med 1992; 21: 177–190.
Rabkin SW, Chen Y, Leiter L, Liu L, Reeder BA. Risk factor correlates of body mass index. Can Med Assoc J 1997; 157(Suppl 1): s26–s31.
Zhang Y, Proenca R, Maffel M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature 1994; 372: 425–432.
Pelleymounter MA, Cullen MJ, Baker MB, et al. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 1995; 269: 540–543.
Auwerx J, Staels B. Leptin. Lancet 1998; 351: 737–742.
Friedman JM. Leptin, leptin receptors, and the control of body weight. Nutr Rev 1998; 56: S38–S46.
Haynes WG, Morgan DA, Walsh SA, Sivitz WI, Mark AL. Cardiovascular consequences of obesity: Role of leptin. Clin Exp Pharmacol Physiol 1998; 25: 65–69.
Friedman JM, Halaas JL. Leptin and the regulation of body weight in mammals. Nature 1998; 395: 763–770.
Considine RV, Sinha MK, Heiman ML, et al. Serum immunoreactive leptin concentrations in normal-weight and obese humans. N Engl J Med 1996; 34: 292–295.
Maffei M, Halaas J, Ravussin E, et al. Leptin levels in human and rodent: Measurement of plasma leptin and Ob RNA in obese and weight-reduced subjects. Nat Med 1995; 1: 1155–1161.
Chu NF, Rimm EB, Wang DJ, Liou HS, Shieh SM. Relationship between anthropometric variable and lipid levels among school children: The Taipei Children Heart Study. Int J Obes 1998; 22: 66–72.
Chu NF, Rimm EB, Wang DJ, Liou HS, Shieh SM. Clustering of cardiovascular disease risk factors among obese schoolchildren: The Taipei Children Heart Study. Am J Clin Nutr 1998; 67: 1141–1146.
Richmond W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem 1973; 19: 1350–1356.
Stavropoulos WS, Crouch RD. A new calorimetric procedure for the determination of serum triglyceride. Clin Chem 1974; 20: 857.
Wamick GR, Benderson J, Albers JJ. Dextran sulfate-Mg precipitation procedure for quanti.cation of high density lipoprotein-cholesterol. Clin Chem 1982; 28: 1379–1388.
Friedewald WT, Levy R, Fredrickson DS. Estimation of the concentration of low density lipoprotein-cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499–502.
Marcovina SM, Albers JJ, Dati F, Ledue TB, Ritchie RF. International federation of clinical chemistry standardization projects for measurements of apolipoprotein A-1 and B. Clin Chem 1991; 37: 1676–1682.
Ma Z, Gingerich RL, Santiago JV, Klein S, Smith CH, Landt M. Radioimmunoassay of leptin in human plasma. Clin Chem 1996; 42: 942–946.
Strong JP, Eggen DA, Oalmann MC. Pathology and epidemiology of atherosclerosis. J Am Diet Assoc 1973; 62: 262–268.
Holman RL, McGill HC, Strong JP, et al. The natural history of atherosclerosis: The early aortic lesions as seen in New Orleans in the middle of the 20th century. Am J Pathol 1958; 34: 209–235.
Porkka KV, Viikari JS, Akerblom HK. Tracking of serum HDL-cholesterol and other lipids in children and adolescents: The Cardiovascular Risk in Young Finns Study. Prev Med 1991; 20: 713–724.
Webber LS, Srinivasan SR, Wattigney WA, Berenson GS. Tracking of serum lipids and lipoproteins from childhood to adulthood: The Bogalusa Heart Study. Am J Epidemiol 1991; 133: 884–899.
Myers L, Coughlin SS, Webber LS, Srinivasan SR, Berenson GS. Prediction of adult cardiovascular multifactorial risk status from childhood risk factor levels: The Bogalusa Heart Study. Am J Epidemiol 1995; 142: 918–924.
Smoak CG, Burke GL, Webber LS, Harsha DW, Srinivasan SR, Berenson GS. Relation of obesity to clustering of cardiovascular disease risk factors in children and young adults. The Bogalusa Heart Study. Am J Epidemiol 1987; 125: 364–372.
Hassink SG, Sheslow DV, de Lancey E, Opentanova I, Considine RV, Caro JF. Serum leptin in children with obesity: Relationship to gender and development. Pediatrics 1996; 98: 201–203.
Clayton PE, Gill MS, Hall CM, Tillmann V, Whatmore AJ, Price DA. Serum leptin through childhood and adolescence. Clin Endocrinol 1997; 46: 727–733.
Ellis KJ, Nicolson MY. Leptin levels and body fatness in children: Effects of gender, ethnicity, and sexual development. Pediatr Res 1997; 42: 484–488.
Blum WF, Englaro P, Hanitsch S, et al. Plasma leptin levels in healthy children and adolescents: Dependence on body mass index, body fat mass, gender, pubertal stage, and testosterone. J Clin Endocrinol Metab 1997; 82: 2904–2910.
Falorni A, Bini V, Molinari D, et al. Leptin serum levels in normal weight and obese children and adolescents: Relationship with age, sex, pubertal development, body mass index and insulin. Int J Obes 1997; 21: 881–890.
Zimmet P, Hodge A, Nicolson M, et al. Serum leptin concentration, obesity, and insulin resistance in Western Samoans: Cross sectional study. Br Med J 1996; 313: 965–969.
Mantzoros CS, Moschos S, Avramopoulos I, et al. Leptin concentrations in relation to body mass index and the tumor necrosis factor-a system in humans. J Clin Endocrinol Metab 1997; 82: 3408–3413.
Vettor R, De Pergola G, Pagano C, et al. Gender differences in serum leptin in obese people: Relationships with testosterone, body fat distribution and insulin sensitivity. Eur J Clin Invest 1997; 27: 1016–1024.
Leyva F, Godsland IF, Ghatei M, et al. Hyperleptinemia as a component of a metabolic syndrome of cardiovascular risk. Arterioscler Thromb Vasc Biol 1998; 18: 928–933.
Liuzzi A, Savia G, Tagliaferri M, et al. Serum leptin concentration in moderate and severe obesity: Relationship with clinical, anthropometric and metabolic factors. Int J Obes Relat Metab Disord 1999; 23: 1066–1073.
Lopez-Soriano J, Carbo N, Lopez-Soriano FJ, Argiles JM. Short-term e.ects of leptin on lipid metabolism in the rat. FEBS 1998; 431: 371–374.
Suga A, Hirano T, Inoue S, et al. Plasma leptin levels and triglyceride secretion rates in VMH-lesioned obese rates: A role of adiposity. Am J Physiol 1999; 276: E650–E657.
Bryson JM, Phuyal JL, Swan V, Caterson ID. Leptin has acute effects on glucose and lipid metabolism in both lean and gold thioglucose-obese mice. Am J Physiol 1999; 277: E417–E422.
Rainwater DL, Comuzzie AG, VandeBerg JL, Mahaney MC, Blangero J. Serum leptin levels are independently correlated with two measures of HDL. Atherosclerosis 1997; 132: 237–243.
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Wu, DM., Shen, MH. & Chu, NF. Relationship between plasma leptin levels and lipid profiles among school children in Taiwan – The Taipei Children Heart Study. Eur J Epidemiol 17, 911–916 (2001). https://doi.org/10.1023/A:1016280427032
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DOI: https://doi.org/10.1023/A:1016280427032