Abstract
A simple viscometric method was used to quantify mucin-polymer bioadhesive bond strength. Viscosities of 15% (w/v) porcine gastric mucin dispersions in 0.1 N HC1 (pH 1) or 0.1 N acetate buffer (pH 5.5) were measured with a Brookfield viscometer in the absence (ηm) or presence (ηt) of selected neutral, anionic, and cationic polymers (0.1–2.5%, w/v). Viscosity components of bioadhesion (1%) were calculated from the equation, ηt = ηm + ηp + ηb, where ηp is the viscosity of corresponding pure polymer solution as measured by an Ostwald viscometer. The forces of bioadhesion (F) were calculated from the equation, F = ηbσ, where σ is the rate of shear/sec. ηb's and F's for polyelectrolytes, e.g., polyacrylic acid, cationic gelatin, and chitosan were always higher in acetate buffer than in HC1. Validity of the technique and the effect of ionic charge, polymer conformation, and rate of shear on ηb and F are discussed, as is a comparison of this method to other methods for evaluating bioadhesive materials.
Similar content being viewed by others
REFERENCES
N. A. Peppas and P. A. Buri. J. Control. Release 2:257–275 (1985).
J. Gu, J. R. Robinson, and S. Leung. CRC Crit. Rev. Ther. Drug Carrier Syst. 5(l):21–67 (1988).
J. D. Smart, I. W. Kellaway, and H. E. C. Worthington. J. Pharm. Pharmacol. 36:295–299 (1984).
H. W. Ch'ng, H. Park, P. Kelley, and J. R. Robinson. J. Pharm. Sci. 74:399–405 (1985).
S. H. S. Leung and J. R. Robinson. J. Control. Release 5:223–231 (1988).
M. Ishida, Y. Machida, N. Nambu, and T. Nagai. Chem. Pharm. Bull. 29:810–816 (1981).
S. Reich, W. Levy, A. Meshores, M. Blumental, J. W. Sheets, and E. P. Goldberg. J. Biomed. Mater. Res. 18:737–744 (1984).
G. P. Anderson, S. J. Bennett, and K. L. DeVries. Analysis and Testing of Adhesive Bonds, Academic Press, New York, 1977.
J. L. Chen and G. N. Cyr. In R. S. Manly (ed.), Adhesives in Biological Systems, Academic Press, New York, 1970, pp. 163–181.
H. J. Galla, W. Hartmann, U. Theilen, and E. Sackmann. J. Membr. Biol. 48:215–236 (1979).
K. Park and J. R. Robinson. Int. J. Pharm. 19:107–127 (1984).
A. G. Mikos and N. A. Peppas. In I. A. Chaudry and C. Thies (eds.), Proc. 13th Int. Symp. Control. Release Bioact. Mater., Controlled Release Society, Lincolnshire, IL, 1986, p. 97.
M. Bohdanecky and J. Kvar. In A. D. Jenkins (ed.), Viscosity of Polymer Solutions, Polymer Science Library 2, Elsevier Scientific, Amsterdam-Oxford-New York, 1982.
J. F. Forstner, I. Jabbal, B. P. Findlay, and G. G. Forstner. Mod. Probl. Paediat. 19:54–65 (1977).
S. J. List, B. P. Findlay, G. G. Forstner, and J. F. Forstner. Biochem. J. 175:565–571 (1978).
M. Saga, H. Hamada, R. M. Nakamura, V. Davajan, and S. Allerton. Sei Marianna Ika Diagaku Zasshi 7:146–150 (1979).
J. F. Bridges, J. F. Woodley, R. Duncan, and J. Kopecek. Int. J. Pharm. 44:213–223 (1988).
K. Park and J. R. Robinson. J. Control. Release 2:47–57 (1985).
A. Katchalsky. Biophys. J. 4:9–41 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hassan, E.E., Gallo, J.M. A Simple Rheological Method for the in Vitro Assessment of Mucin-Polymer Bioadhesive Bond Strength. Pharm Res 7, 491–495 (1990). https://doi.org/10.1023/A:1015812615635
Issue Date:
DOI: https://doi.org/10.1023/A:1015812615635