Abstract
Hairy root cultures of Artemisia annua L were cultivated for 30 days under either white, red, blue, yellow or green light. Red light at 660 nm gave the highest biomass of hairy roots (5.73 g dry wt cells l−1 medium) and artemisinin content (31 mg arteminsinin g−1 dry cells) which were, respectively, 17% and 67% higher than those obtained under white light.
Similar content being viewed by others
References
Cai GQ, Li GZ, Ye HC, Li GF (1995) Hairy root culture of Artemisia annua L by plasmid transformation and biosynthesis of artemisinin. Chin. J. Biotech. 11: 315-320.
Dubois M,Gilles KA,Hamilton JK,Revers PA,Smith F (1956) Colorimetric methods for the determination of sugar and related substances. Anal. Chem. 28: 350-357.
Liu CZ,Wang YC,Kang XZ,Ouyang F (1999) Investigation of light, temperature and cultivated modes on growth and artemisinin synthesis of Artemisia annua L shoots. Acta Phytophy. Sin. 25: 105-109.
Loveys BR,Wareing PF (1971) The red light controlled production of gibberellin in etiolated wheat leaves. Planta 98: 109-116.
Mohr H,Schopfer P (1995) Photomorphogenesis. Plant Physiology. Berlin: Springer-Verlag, pp. 345-369.
Shen XK,Yan KD,Luo ZY (1983) Determination of artemisinin content by ultraviolet meter. Chin. Pharm. Anal. 3: 24-26.
Weathers PJ,Cheetham RD,Follansbee E,Teoh K (1997) Artemisinin production by transformed roots of Artemisia annua L. Biotechnol. Lett. 19: 927-929.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, Y., Zhang, H., Zhao, B. et al. Improved growth of Artemisia annua L hairy roots and artemisinin production under red light conditions. Biotechnology Letters 23, 1971–1973 (2001). https://doi.org/10.1023/A:1013786332363
Issue Date:
DOI: https://doi.org/10.1023/A:1013786332363