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Localization of Thiamine-Binding Protein in Synaptosomes from the Rat Brain

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Abstract

Using preparations of synaptosomes and subsynaptosomal fractions from the rat brain, we studied the localization of thiamine-binding protein (TBP) in the subcellular structures of the neurons. In addition, we studied the distribution in synaptosomes of two types of activity typical of TBP (thiamine triphosphatase and thiamine-binding activities), as well as the effects of factors destroying the plasma membrane of synaptosomes on binding of [14C]thiamine with the latter. We found that the thiamine-associated activity of TBP was the highest in fractions of the synaptic vesicles and plasma membranes. Hydrolysis of thiamine triphosphate was also most active in these structures. Our results allow us to conclude that TBP is localized mostly in the synaptic vesicles and plasma membranes of synaptosomes.

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REFERENCES

  1. Yu. M. Parkhomenko, Z. S. Protasova, and G. V. Donchenko, “Neuroactivity of thiamine: facts and hypotheses,” Ukr. Biokhim. Zh., 68, No. 2, 3-15 (1996).

    Google Scholar 

  2. H. Haas, “Thiamine and the brain,” Ann. Rev. Nutr., 8, 483-515 (1988).

    Google Scholar 

  3. V. A. Postoenko, Yu. M. Parkhomenko, A. I. Vovk, et al., “Pu-rification and some properties of synaptosomal thiamine-bind-ing protein from the rat brain,” Biokhimiya, 52, No. 11, 1792-1797 (1987).

    Google Scholar 

  4. V. A. Postoenko, Yu. M. Parkhomenko, and G. V. Donchen-ko, “Characteristics of synaptosomal thiamine-binding pro tein from the rat brain,” Ukr. Biokhim. Zh., 59, No. 6, 9-14 (1987).

    Google Scholar 

  5. V. A. Postoenko, Yu. M. Parkhomenko, and G. V. Donchenko, “Study of amino acid contents and nonprotein components of synaptosomal thiamine-binding protein from the rat brain,” Neirokhimiya, 9, No. 1, 29-34 (1990).

    Google Scholar 

  6. .J. Abita, R. Chicheporiticke, and H. Schwetz, “Effect of neuro-toxins (veratridine, sea anemone toxin, tetrodotoxin) on transmitter accumulation and release by nerve terminals in vitro,” Biochem. J., 16, No. 9, 1838-1869 (1977).

    Google Scholar 

  7. S. W. Henn and H. Fritz, “The identification of subcellular fractions of the central nervous system,” in: Handbook of Neurochemistry, Vol. 2, Experimental Neurochemistry, Plenum Press, New York, London (1982), pp. 147-162.

    Google Scholar 

  8. Kwok-Mind Chan, D. Delfart, and D. Kurt, “A direct colori-metric assay for Ca 2+-stimulated ATPase activity,” Analyt. Bio-chem., 157, No. 2, 375-380 (1986).

    Google Scholar 

  9. R. L. Barchi and P. E. Brown, “Thiamine triphosphatase in the ner-vous system,” Biochem. Biophys. Acta, 255, No. 1, 402-409 (1972).

    Google Scholar 

  10. R. L. Barchi and R. O. Viale, “Membrane-associated thiamine triphosphatase. II. Activation by divalent cations, J. Biol. Chem., 251, No. 1, 193-197 (1976).

    Google Scholar 

  11. H. K. Penttinen and L. Uotila, “The relation of the soluble thiamine triphosphatase activity of various rat tissues to non-specific phosphatases,” Med. Biol., 59, No. 3, 177-184 (1981).

    Google Scholar 

  12. L. Bettendorff, C. Michel-Cahay, and Ch. Grandfils, “Thd membrane-associated thiamine phos-phatase in the electric organ of Electrophorus electricus,” J. Neurochem., 49, No. 2, 495-502 (1987).

    Google Scholar 

  13. Sh.-ich. Sano, Y. Matsuda, and H. Nakagawa, “Type B nucleo-side-diphosphatase of rat brain: purification and properties of an enzyme with high thiamine pyrophosphatase activity,” Mem. Inst. Protein Res. Osaka Univ., 31, 125-133 (1989).

    Google Scholar 

  14. W. Ovtscharoff and St. Manolov, “Cytochemical localization of adenosine triphosphatase and thiamine pyrophosphatase in the synapses of rat's cerebral cortex,” Acta Histochem., 60, No. 2, 329-338 (1977).

    Google Scholar 

  15. J. A. Evans, “Thiamine pyrophosphatase localization in neuronal àxons and terminals,” J. Histochem. Cytochem., 30, No. 5, 489-490 (1982).

    Google Scholar 

  16. A. Duggleby, “A nonlinear regression program for small com-puters,” Analyt. Biochem., 110, No. 1, 9-18 (1981).

    Google Scholar 

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Authors and Affiliations

  1. National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry, Kyiv, Ukraine

    Yu. M. Parkhomenko, Z. S. Protasova, O. R. Yanchiy, K. Khosla & G. V. Donchenko

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  1. Yu. M. Parkhomenko
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  2. Z. S. Protasova
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  3. O. R. Yanchiy
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  4. K. Khosla
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  5. G. V. Donchenko
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Parkhomenko, Y.M., Protasova, Z.S., Yanchiy, O.R. et al. Localization of Thiamine-Binding Protein in Synaptosomes from the Rat Brain. Neurophysiology 33, 135–139 (2001). https://doi.org/10.1023/A:1012840417285

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