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High frequency adventitious shoot induction and plant regeneration from leaves of statice

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Abstract

An efficient regeneration system for large-scale propagation of statice (Limonium altaica cv. Emille) was developed using leaves from mature plants. Leaf segments (5×5 mm sections) were cultured on Murashige and Skoog's medium supplemented with N6-benzyladenine (BA) and thidiazuron (TDZ) individually and in combination with indole-3-acetic acid (IAA) and α-naphthaleneacetic acid (NAA). Prolific direct adventitious shoot regeneration occurred on most of the media. The best response in terms of frequency of shoot regeneration (99.5%) and number of shoots per explant (112 shoots per explant) was observed on medium supplemented with 2.85 μM IAA and 1.14 μM TDZ. The shoots rooted easily on half strength MS medium and MS medium with indole-3-butyric acid. In vitro propagated plants could be transferred to soil with survival rates of more than 95%.

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 316-763, Cheongju, Republic of Korea

    J.H. Jeong & K.Y. Paek

  2. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 316-763, Cheongju, Republic of Korea

    H.N. Murthy

  3. Department of Botany, Karnatak University, Dharwad –, 580 003, India

    H.N. Murthy

Authors
  1. J.H. Jeong
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  2. H.N. Murthy
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  3. K.Y. Paek
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Correspondence to K.Y. Paek.

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Jeong, J., Murthy, H. & Paek, K. High frequency adventitious shoot induction and plant regeneration from leaves of statice. Plant Cell, Tissue and Organ Culture 65, 123–128 (2001). https://doi.org/10.1023/A:1010697207189

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