Abstract
When different seedling explants, i.e. hypocotyl, epicotyl, cotyledonary node, root-shoot zone, cotyledon, leaves and roots from 7-day-old seedlings of neem were cultured on Murashige and Skoog's medium supplemented with 2 mg l−1 benzyladenine and 0.1 mg l−1indole-3-acetic acid, shoot buds were initiated from all the explants tested, with leaf explants producing the highest average number of shoots/explant. The regenerated shoots were further subcultured and later could be rooted on a medium supplemented with indole butyric acid (1 mg l−1) and complete plants could be obtained.
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Salvi, N.D., Singh, H., Tivarekar, S. et al. Plant regeneration from different explants of neem. Plant Cell, Tissue and Organ Culture 65, 159–162 (2001). https://doi.org/10.1023/A:1010672809141
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DOI: https://doi.org/10.1023/A:1010672809141