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Development of shoots and roots in anther-derived callus of Azadirachta indica A. Juss.—a medicinal tree

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Abstract

Callus originated in microsporangial wall layers and connective tissues of anthers containing uninucleate microspores on Nitsch's or Murashige and Skoog's medium supplemented with growth regulators. A higher percentage of cultures (43) produced callus on Nitsch's medium containing 10 μM indole-3-acetic acid + 1 μM 6-benzyladenine. After 13–15 weeks, green nodular structures and prominent roots developed in 25% of the cultures on Murashige and Skoog's medium + 10 μM α-naphthaleneacetic acid + 1 μM kinetin. Multiple shoots were induced in this anther-derived callus when subcultured on Murashige and Skoog's medium augmented with 4.44 μM 6-benzyladenine + 0.53 μM α-naphthaleneacetic acid along with 18.75 μM polyvinylpyrrolidone. The excised shoots formed roots after subculturing on Murashige and Skoog's medium + 4.90 μM indole-3-butyric acid + 18.75 μM polyvinylpyrrolidone, thus developing complete plantlets. Examination of callusing anthers also revealed two- to multi-celled pollen masses with intact exine.

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Abbreviations

BA:

6-benzyladenine

CW:

coconut water

2,4-d :

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

HCl:

hydrochloric acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KMnO4 :

potassium permanganate

MS:

Murashige & Skoog's medium

NAA:

α-naphthaleneacetic acid

NB:

Nitsch's medium

PVP:

polyvinylpyrrolidone

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Authors and Affiliations

  1. Department of Botany, University of Delhi, 110007, Delhi, India

    Virendra K. Gautam & Shrish C. Gupta

  2. Department of Botany, Daulat Ram College, University of Delhi, 110007, Delhi, India

    Kanan Nanda

Authors
  1. Virendra K. Gautam
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  2. Kanan Nanda
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  3. Shrish C. Gupta
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Gautam, V.K., Nanda, K. & Gupta, S.C. Development of shoots and roots in anther-derived callus of Azadirachta indica A. Juss.—a medicinal tree. Plant Cell Tiss Organ Cult 34, 13–18 (1993). https://doi.org/10.1007/BF00048458

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  • DOI: https://doi.org/10.1007/BF00048458

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