Skip to main content
SpringerLink
Log in

Interaction between Duodenase and α1-Proteinase Inhibitor

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

The interaction between duodenase, a newly recognized serine proteinase belonging to the small group of Janusfaced proteinases, and α1-proteinase inhibitor (α1-PI) from human serum was investigated. The stoichiometry of the inhibition was 1.2 mol/mol. The presence of a stable enzyme–inhibitor complex was shown by SDS-PAGE. The mechanism of interaction between duodenase and α1-PI was shown to be of the suicide type. The equilibrium and inhibition constants are 13 ± 3 nM and (1.9 ± 0.3)·105 M–1·sec–1, respectively. Based on the association rate constant of the enzyme–inhibitor complex and localization of duodenase and α1-PI in identical compartments, α1-PI is suggested to be a duodenase inhibitor in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Zamolodchikova, T. S., Sokolova, E. A., Alexandrov, S. L., Mikhaleva, I. I., Prudchenko, I. A., Morozov, I. A., Kononenko, N. V., Mirgorodskaya, O. A., Da, U., Larionova, N. I., Pozdnev, V. F., Ghosh, D., Duax, W. L., and Vorotyntseva, T. I. (1997) Eur. J. Biochem., 249, 612–621.

    Google Scholar 

  2. Zamolodchikova, T. S., Sokolova, E. A., Deshun, L., and Sadler, J. E. (2000) FEBS Lett., 466, 295–299.

    Google Scholar 

  3. Matshushita, M., Ichinose, M., and Yahagi, N. (1994) J. Biol. Chem., 269, 19976–19982.

    Google Scholar 

  4. Gladysheva, I. P., Zamolodchikova, T. S., Sokolova, E. A., and Larionova, N. I. (1999) Biochemistry(Moscow), 64, 1244–1249.

    Google Scholar 

  5. Travis, J., and Salvesen, G. (1983) Ann. Rev. Biochem., 52, 655–709.

    Google Scholar 

  6. Steinbuch, M. (1979) Coll. Ges. Biol. Chem., 30, 207–222.

    Google Scholar 

  7. Chase, T., and Shaw, E. (1967) Biochem. Biophys. Res. Commun., 269, 508–514.

    Google Scholar 

  8. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem., 193, 265–275.

    Google Scholar 

  9. Gladysheva, I. P., Balabushevich, N. G., Moroz, N. A., and Larionova, N. I. (2000) Biochemistry(Moscow), 65, 198–203.

    Google Scholar 

  10. Klesov, A. A., and Berezin, I. V. (1976) Enzymatic Kinetics: a Practical Guide[in Russian], MGU Publishers, Moscow, pp. 79, 168, 176.

  11. Duranton, J., Adam, C., and Bieth, J. G. (1998) Biochemistry, 37, 11239–11245.

    Google Scholar 

  12. Laemmli, U. K. (1970) Nature(London), 227, 680–685.

    Google Scholar 

  13. Cooperman, B. S., Stavridi, E., Nickbarg, E., Rescorla, E., Schechter, N. M., and Rubin, H. (1993) J. Biol. Chem., 268, 23616–23625.

    Google Scholar 

  14. Bieth, J. (1986) Regulation of Matrix Accumulation, Academic Press, New York, pp. 217–320.

  15. Berniger, R. W. (1986) J. Med. Exp. Clin., 16, 101–128.

    Google Scholar 

  16. Desrochers, P. E., Mookhtiar, K., van Wart, H. E., Hasty, K. A., and Weiss, S. J. (1992) J. Biol. Chem., 267, 5005–5012.

    Google Scholar 

  17. Mast, A. E., Enghild, J. J., Nagase, H., Suzuki, K., Pizzo, S. V., and Salvesen, G. (1991) J. Biol. Chem., 266, 15810–15816.

    Google Scholar 

  18. Schechter, N. M., Sprows, J. L., Schoenberger, O. L., Lazarus, G. S., Cooperman, B. S., and Rubin, H. (1989) J. Biol. Chem., 264, 21308–21315.

    Google Scholar 

  19. Salvesen, G., Farley, D., Shuman, J., Przybyla, A., Reilly, C., and Travis, J. (1987) Biochemistry, 26, 2289–2293.

    Google Scholar 

  20. Pletnev, V. Z., Zamolodchikova, T. S., Pangborn, W. A., and Duax, W. L. (2000) Proteins, 41, 8–16.

    Google Scholar 

  21. Pemberton, A. D., Belham, C. M., Huntley, J. F., Plevin, R., and Miller, R. P. (1997) Biochem. J., 323, 719–725.

    Google Scholar 

  22. Pemberton, A. D., Huntley, J. F., and Miller, R. P. (1997) Biochem. J., 321, 665–670.

    Google Scholar 

  23. Mistry, R., Snashall, P. D., Totty, N., Guz, A., and Telley, T. D. (1991) Biochem. J., 273, 685–690.

    Google Scholar 

  24. Saklatvala, J., and Wood, G. C. (1976) Protides Biol. Fluids, 23, 99–102.

    Google Scholar 

  25. Cichy, J., Potempa, J., and Travis, J. (1993) J. Biol. Chem., 272, 8250–8255.

    Google Scholar 

  26. Malik, A. K., and McGee, J. O. (1993) Malays. J. Pathol., 15, 151–154.

    Google Scholar 

  27. Geboes, K., Rutgeerts, P., Vantrappen, G., and Desmet, V. J. (1983) Hepatogastroenterology, 30, 24–26.

    Google Scholar 

  28. Kittas, C., Aroni, K., Matani, A., and Papadimitriou, C. S. (1982) Hepatogastroenterology, 29, 275–277.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia

    I. P. Gladysheva, N. A. Popykina & N. I. Larionova

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

    T. S. Zamolodchikova

Authors
  1. I. P. Gladysheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. A. Popykina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. S. Zamolodchikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. I. Larionova
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gladysheva, I.P., Popykina, N.A., Zamolodchikova, T.S. et al. Interaction between Duodenase and α1-Proteinase Inhibitor. Biochemistry (Moscow) 66, 682–687 (2001). https://doi.org/10.1023/A:1010267500296

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010267500296

Search

Navigation