Abstract
A polygalacturonase was purified from the thermophilic fungus, Thermomyces lanuginosus to apparent homogeneity by ultrafiltration, acetone precipitation and ion-exchange chromatography. The enzyme was maximally active at pH 5.5 and 60 °C. The apparent KM with potassium pectate was 0.67 mg/ml and the Vmax was 7.2 × 105 μmol/min/mg protein. The apparent molecular weight of the enzyme was 59 kDa and it contained approximately 10% carbohydrate. The enzyme was completely stable at room temperature (32 ± 3 °C) and retained about 50% activity at 50 °C for 6 h. The zymogram of the purified enzyme revealed two activity bands, one of which was a major one. Polyclonal antibodies raised against the enzyme did not show any immunological relatedness with other mesophilic polygalacturonases.
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Kumar, S.S., Palanivelu, P. Purification and characterization of an extracellular polygalacturonase from the thermophilic fungus, Thermomyces lanuginosus. World Journal of Microbiology and Biotechnology 15, 643–646 (1999). https://doi.org/10.1023/A:1008998523125
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DOI: https://doi.org/10.1023/A:1008998523125