Skip to main content
SpringerLink
Log in

The processing and characterization of animal-derived bone to yield materials with biomedical applications. Part II: milled bone powders, reprecipitated hydroxyapatite and the potential uses of these materials

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Further studies on the processing and use of animal-bone-derived calcium phosphate materials in biomedical applications are presented. Bone powders sourced either from the direct crushing and milling of bovine, ovine and cervine bone or after being subjected to defatting and acid digestion/NaOH reprecipitation and sodium hypochlorite hydrogen peroxide treatment of animal bones were characterized using Fourier transform infra-red (FTIR) spectroscopy, 13C solid state magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy, atomic absorption (AA) and inductively coupled plasma (ICP) spectrometric techniques. Bone powders were trialled for their potential use as a substrate for phosphine coupling and enzyme immobilization as well as a feedstock powder for plasma spraying on titanium metal substrates. Results indicated that enzyme immobilization by phosphine coupling could be successfully achieved on milled cervine bone with the immobilized enzyme retaining some activity. It was found that the presence of impurities normally carried down with the processing of the bone materials (viz., fat and collagen) played an important role in influencing the adsorbency and reactivity of the powders. Plasma spraying studies using reprecipitated bovine-derived powders produced highly adherent coatings on titanium metal, the composition of which was mostly hydroxyapatite(Ca10(PO4)6(OH)2) with low levels of α-tricalcium phosphate (α-Ca3(PO4)2) and tetracalcium phosphate (Ca4P2O9) also detected. In general, animal derived calcium phosphate materials constitute a potentially cheaper source of calcium phosphate materials for biomedical applications and make use of a largely under-utilized resource from abattoir wastes. © 2000 Kluwer Academic Publishers

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. SAVARINO, S. STEA, D. GRANCHI, M. E. DONATI, M. CERVELLATI, A. MORONI, G. PAGANETTO and A. PIZZOFERRATO, J. Mater. Sci: Mater. Med. 9 (1998) 109.

    Google Scholar 

  2. P. FRAYSSINET, E. ASIMUS, A. AUTEFAGE and J. FAGES, ibid. 6 (1995) 473.

    Google Scholar 

  3. B. F. YETER-DAL, V. GROSS, and T. W. TURNEY in “Ceramics, adding the value, Volume 2” Proceedings of the International Ceramic Conference, Australia, 1992, (CSIRO Publications, Melbourne, 1992) p. 617.

    Google Scholar 

  4. Composition of Ceramic Hydroxylapatite for Surgical Implants, ASTEMF 1185-88.

  5. G. S. JOHNSON, M. R. MUCALO and M. A. LORIER, J. Mater. Sci: Mater. Med., in press.

  6. AOAC International 16th edn (Association of Official Analytical Chemists), 2 (1995) Ch. 39, p. 7.

  7. F. C. COCHRANE, H. H. PETACH and W. HENDERSON, Enzyme and Microbial Technology, 18 (1996) 373.

    Google Scholar 

  8. United States Pharmacopoeia (USP) Twenty First Revision (1985), United States Pharmacopoeial Convention Inc., Maryland, p. 1104.

  9. S. KANO, A. YAMAZAKI, R. OTSUKA, M. OHGAKI, S. NAKAMURA, M. AKAO and H. AOKI, Apatite Volume 1, Proceedings of the First International Symposium on Apatite, Mishima, Japan, July 1991 (Japanese Association of Apatite Science, March, 1992) 91.

  10. Powder Diffraction Files, Pattern numbers: 4-0777 (CaO), 5-0628 (NaCl), 25-1137 (Ca4P2O9) and 9-348 (a-Ca3(PO4)2) Joint Committee on Powder Diffraction Standards, Philadelphia, 1974.

  11. W. KEMP in “NMR in Chemistry, A Multinuclear Introduction”, (Macmillan Education Ltd, London, 1986) p. 217.

    Google Scholar 

  12. E. D. WEIL in “Handbook of Organophosphorus Chemistry” edited by R. Engel (Dekker, New York, 1992) Ch. 14.

    Google Scholar 

  13. W. HENDERSON, G. M. OLSEN and L. S. BONNINGTON, J. Chem. Soc. Chem. Commun. (1994) 1863.

  14. H. H. PETACH, W. HENDERSON and G. M. OLSEN, ibid. (1994) 2181.

  15. W. HENDERSON, H. H. PETACH and L. S. BONNINGTON, Eur. Poly J. 31 (1994) 981.

    Google Scholar 

  16. W. HENDERSON, Chemistry Department, University of Waikato, Private Communication.

  17. J. EVERSE, C. L. GINZBURGH and N. O. KAPLAN in “Methods of Biochemical Analysis” edited by C. Suelter, (Wiley, New York, 1981) p. 135.

    Google Scholar 

  18. R. MCPHERSON, N. GANE and T. J. BASTOW, J. Mater. Sci: Mater. Med. 6 (1995) 327.

    Google Scholar 

  19. L. HENCH and J. WILSON in “An Introduction to Bioceramics, Advanced Series in Ceramics-Vol. 1”, (World Scientific, Singapore, 1993) p. 154, 199.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemistry Department, University of Waikato, Private Bag 3105, Hamilton, New Zealand

    G. S. Johnson

  2. Chemistry Department, University of Waikato, Private Bag 3105, Hamilton, New Zealand

    M. R. Mucalo

  3. MIRINZ Food Technology and Research Ltd, P.O. Box 617, Hamilton, New Zealand

    M. A. Lorier

  4. Institut fuer Verbundwerkstoffe, Chemnitz Technical University, Erfenschalger Str. 73, D-09125, Chemnitz, Germany

    U. Gieland & H. Mucha

Authors
  1. G. S. Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. R. Mucalo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. A. Lorier
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. U. Gieland
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Mucha
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Johnson, G.S., Mucalo, M.R., Lorier, M.A. et al. The processing and characterization of animal-derived bone to yield materials with biomedical applications. Part II: milled bone powders, reprecipitated hydroxyapatite and the potential uses of these materials. Journal of Materials Science: Materials in Medicine 11, 725–741 (2000). https://doi.org/10.1023/A:1008979929632

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008979929632

Keywords

Search

Navigation