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Preparation and Microstructural Characterization of Natural Hydroxyapatite Extracted from Animal Bones

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Abstract

An attempt has been made to optimize the heating treatment temperature of a fresh bovine bone sample using thermogravimetric and differential thermal analysis (TGA/DTA) between room temperature and 1000°C in atmospheric air. It has been shown that the organic matter and carbonate have been totally removed from the washed bone powder after calcining at 800°C. To investigate the effect of the residence time of the thermal treatment at 800°C on the crystallinity and the morphology of the hydroxyapatite (HA) samples, FTIR-ATR produced crystalline indices (CIs) calculated for 1 h, 6 h, 12 h, 24 h and 48 h as holding times are compared to those obtained through XRD. Scanning electron microscopy (SEM) and energy-dispersive x-ray (EDX) analyses were also performed. The CI result shows that, quantitatively speaking, the values obtained by these two techniques were not comparable although the general trends were similar. From SEM pictures and the corresponding EDX results, the samples subjected to calcination at 800°C had relatively the same sized spherical morphology and the same Ca/P ratios close to the standard one (≈ 1.67) regardless of the preparative calcining time.

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Acknowledgements

The Ministry of Higher Education and Scientific Research of Tunisia supported this study financially. The authors thank Mrs. Hayet Werda for proof-reading, correcting and improving the English of the manuscript.

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  1. Laboratory of Advanced Materials, National School of Engineering, Box 1173, 3038, Sfax, Tunisia

    Aymen Ayadi & Jamel Bouaziz

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  1. Aymen Ayadi
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Ayadi, A., Bouaziz, J. Preparation and Microstructural Characterization of Natural Hydroxyapatite Extracted from Animal Bones. JOM 74, 3119–3132 (2022). https://doi.org/10.1007/s11837-022-05364-3

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