Abstract
The objective of this study was to explore the molecular mechanisms of adhesion of endothelial cells (ECs) to implant grades of titanium alloy (Ti) and stainless steel (SS), compared to tissue culture polystyrene (PS). The idea is that promotion of EC adhesion to implant surfaces during the initial stages of healing may be critical in the formation of a capillary bed intimately associated with the implant surface. Ultimately this could be expected in turn to promote bone formation close to the surface and a more stable implant/bone interface. Surfaces were coated with either peak 1 fibrinogen γAγA, fibrinogen Fr I-9, fibrinogen fragment D1, fibronectin, vitronectin, or fetal calf serum and then post-coated with bovine serum albumin (BSA) to block non-specific cell adhesion. Surfaces with BSA alone and no other protein coating were also evaluated. Fibronectin coating maximized cell adhesion on all three surfaces, and adhesion was highest on PS. BSA blocked cell adhesion to PS (and most adhesion to SS) much better than to Ti. These results provide evidence that BSA adsorption on the metal surface is unable to effectively block the adhesion of the cells to the Ti. These data may provide a basis for understanding in vivo observations that soft tissue becomes attached to a Ti surface more rapidly and with more bone formation than to SS. Evidence is also presented that αvβ3 plays an important role in adhesion of ECs to the Ti surface. These experiments also provide preliminary data which may reflect some of the features of initial EC adhesion to metal implants. ©2000 Kluwer Academic Publishers
Similar content being viewed by others
References
K. D. CHESMEL, C. C. CLARK, C. T. BRIGHTON and J. BLACK, J. Biomed. Mater. Res. 29 (1995) 1101.
G. GRONOWICZ and M. B. MCCARTHY, in “Transactions of the 41st Annual Meeting Orthopaedic Research Society”, Orlando, FL, Orthopaedic Research Society, Richard A. Brand (ed.), February, 1995, p. 782.
G. GRONOWICZ and M. B. MCCARTHY, J. Orthop. Res. 14 (1996) 878.
S. KAWAGUCHI and U. TOSHIMITSU, ibid. 11 (1993) 386.
R. KORNU, W. J. MALONEY, M. A. KELLY and R. L. SMITH, ibid. 14 (1996) 871.
R. K. SINHA, F. MORRIS, S. A. SHAH and R. S. TUAN, Clin. Orthop. 305 (1994) 258.
R. K. SINHA and R. S. TUAN in “Transactions of the 41st Annual Meeting Orthopaedic Research Society”, Orlando, FL, Orthopaedic Research Society, Richard A. Brand (ed.), February, 1995, p. 139.
S. VERRIER, R. BAREILLE, A. ROVIRA, M. DARD and J. AMEDEE, 12th Europ. Conf. on Biomaterials, Portu, Portugal, European Society for Biomaterials, M.A. Barbosa (Chairman), (1995) 190.
K. D. CHESMEL and J. BLACK, J. Biomed. Mater. Res. 29 (1995) 1089.
V. H. PEREZ-LUNA, T. A. HORBETT and B. D. RATNER, ibid. 28 (1994) 1111.
A. KEITH, in “Proceedings of the Royal Society of Medicine (Section of Surgery)” T. Watts Eden and Hugh Thursfield (eds.), (Longmans, Green and Co., Ltd., Paternoster Row, London) 21 (1927) 301.
J. TRUETA, J. Bone J. Res. 45 (1963) 402.
W. J. CLIFF, Philos. Trans. R. Soc. London. (Biol. Sci.) 246 (1963) 305.
L. OSBORN, Cell 62 (1990) 3.
J. S. POBER and R. S. COTRAN, Transplantation 50 (1990) 537.
Idem., Lab. Invest. 64 (1991) 301.
N. AL-SAFFAR and P. A. REVELL, Brit. J. Rheumatol. 33 (1994) 309.
W. A. JIRANEK, M. MACHADO, M. JASTY, D. JEVSEVAR, H. J. WOLFE, S. R. GOLDRING and M. J. GOLDBERG, J. Bone Joint Surg. [Am] 75A (1993) 863.
N. AL-SAFFAR, Y. KADOYA and P. REVELL, J. Mater. Sci: Mater. Med. 5 (1994) 813.
R. A. SMITH, M. W. MOSESSON, M. M. ROONEY, S. T. LORD, A. U. DANIELS and T. K. GARTNER, J. Biol. Chem. 272 (1997) 22080.
T. J. PODOR, S. A. CURRIDEN and D. J. LOSKUTOFF, in “Endothelial Cells” edited by U. Ryan, (CRC Press, Boca Raton, 1988) Vol I, pp. 127–148.
M.-C. CHANG, B.-R. WANG and T. F. HUANG, Thromb. Haemost. 74 (1995) 764.
C. S. CHEN and J. HAWIGER, Blood 77 (1991) 2200.
L. R. LANGUINO, J. PLESCIA, A. DUPERRAY, A. A. BRIAN, E. F. PLOW, J. E. GELTOSKY and D. C. ALTIERI, Cell 73 (1993) 1423.
D. L. MOORADIAN, P. TRESCONY, K. KEENEY and L. T. FURCHT, J. Surg. Res. 53 (1992) 74.
S. M. ALBELDA, M. DAISE, E. M. LEVIN and C. A. BUCK, J. Clin. Invest. 83 (1989) 1992.
D. A. LAUFFENBURGER and A. F. HORWITZ, Cell 84 (1996) 359.
M. CHRISTOFIDOU-SOLOMIDOU, M. BRIDGES, G. F. MURPHY, S. M. ALBELDA and H. M. DELISSER, Am. J. Path. 151 (1997) 975.
R. A. F. CLARK and R. B. COLVIN, in “Wound repair in plasma fibronectin structure and function”, J. McDonagh (ed.), (Marcel Dekker, Inc., New York, 1985) pp. 197–243.
B. FELDING-HABERMANN and D. A. CHERESH, Curr. Opin. Cell Biol. 5 (1993) 864.
C. T. BRIGHTON and S. M. ALBELDA, J. Orthop. Res. 10 (1992) 766.
M. W. MOSESSON, D. K. GALANAKIS and J. F. FINLAYSON, J. Biol. Chem. 249 (1974) 4656.
D. A. CHERESH, Proc. Natl. Acad. Sci. USA 84 (1987) 6471.
J. S. BENNETT, S. J. SHATTIL, J. W. POWER and T. K. GARTNER, J. Biol. Chem. 263 (1988) 12948.
C. R. HOWLETT, M. D. M. EVANS, W. R. WALSH, G. JOHNSON and J. G. STEELE, Biomater. 15 (1994) 213.
S. M. TAUBENFELD, Y. SONG, D. SHENG, E. L. BALL and G. R. MATSUEDA, Thromb. Heamost. 74 (1995) 923.
D. A. CHERESH, S. A. BERLINER, V. VICENTE and Z. M. RUGGERI, Cell 58 (1989) 945.
L. JENNINGS, D. R. PHILLIPS and W. S. WALKER, Blood 65 (1985) 1112.
Z. WERB, P. M. TREMBLE, O. BEHRENDTSEN, E. CROWLEY and C. H. DAMSKY, J. Cell Biol. 109 (1989) 877.
J. HAPGOOD, T. A. LIBERMANN, I. LAX, Y. YARDEN, A. B. SCHREIBER, Z. NAOR and J. SCHLESSINGER, Proc. Natl. Acad. Sci. 80 (1983) 6451.
E. J. PODESTA, A. R. SOLANO, R. ATTAR, M. L. SANCHEZ and L. M. Y. VEDIA, ibid. 80 (1983) 3986.
Y. SCHECHTER, L. HERNAEZ, J. SCHLESSINGER and P. CUATRECASAS, Nature 278 (1979) 835.
M. A. SOOS, R. M. O'BRIEN, N. P. J. BRINDLE, J. M. STIGTER, A. K. OKAMOTO, J. WHITTAKER and K. SIDDLE, Proc. Natl. Acad. Sci. 86 (1989) 5217.
A. L. FRELINGER III, X. DU, E. F. PLOW and M. H. GINSBERG, J. Biol. Chem. 266 (1991) 17106.
M.-M. HUANG, L. LIPFERT, M. CUNNINGHAM, J. S. BRUGGE, M. H. GINSBERG and S. J. SHATTIL, J. Cell Biol. 122 (1993) 473.
J. O. GALANTE, J. LEMONS, M. SPECTOR, P. D. WILSON and T. M. WRIGHT, J. Orthop. Res. 9 (1991) 760.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Smith, R.A., Mosesson, M.W., Daniels, A.U. et al. Adhesion of microvascular endothelial cells to metallic implant surfaces. Journal of Materials Science: Materials in Medicine 11, 279–285 (2000). https://doi.org/10.1023/A:1008953112683
Issue Date:
DOI: https://doi.org/10.1023/A:1008953112683