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Secondary screening of transconjugant clones mediated by a lytic bacteriophage

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Biotechnology Techniques

Abstract

D.D.G.M. and A.L.Z each contributed to 50%.

Due to the lack of suitable chromosomal markers, antibiotics could not be used to select transconjugant clones arising from matings between two Pseudomonas strains. As an alternative, a method based on the specificity of a lytic bacteriophage for the recipient strain was used successfully. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Laboratoire de Technologie Enzymatique, UPRES A CNRS 6022, Université de Technologie de Compiègne, Centre de Recherches de Royallieu, Compiègne-Cedex, France

    Denis D.G. Mater, Annabelle L. Zgoda, Jean-Noél Barbotin & Daniel Thomas

Authors
  1. Denis D.G. Mater
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  2. Annabelle L. Zgoda
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  3. Jean-Noél Barbotin
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  4. Daniel Thomas
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Mater, D.D., Zgoda, A.L., Barbotin, JN. et al. Secondary screening of transconjugant clones mediated by a lytic bacteriophage. Biotechnology Techniques 12, 811–814 (1998). https://doi.org/10.1023/A:1008876920817

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  • DOI: https://doi.org/10.1023/A:1008876920817

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